Partner: Agnieszka Gadomska-Gajadhur

Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)

Recent publications
1.Denis P., Wrzecionek M., Gadomska‐Gajadhur A., Sajkiewicz P., Poly(Glycerol Sebacate)–Poly(l-Lactide) Nonwovens. Towards Attractive Electrospun Material for Tissue Engineering, Polymers, ISSN: 2073-4360, DOI: 10.3390/polym11122113, Vol.11, pp.2113-1-30, 2019
Abstract:

Two types of poly(glycerol sebacate) (PGS) prepolymers were synthesized and electrospun with poly(l-lactic acid) (PLA), resulting in bicomponent nonwovens. The obtained materials were pre-heated in a vacuum, at different times, to crosslink PGS and investigate morphological and structural dependencies in that polymeric, electrospun system. As both PGS and PLA are sensitive to pre-heating (crosslinking) conditions, research concerns both components. More interest is focused on the properties of PGS, considering further research for mechanical properties and subsequent experiments with PGS synthesis. Electrospinning of PGS blended with PLA does not bring difficulties, but obtaining elastomeric properties of nonwovens is problematic. Even though PGS has many potential advantages over other polyesters when soft tissue engineering is considered, its full utilization via the electrospinning process is much harder in practice. Further investigations are ongoing, especially with the promising PGS prepolymer with a higher esterification degree and its variations.

Keywords:

electrospinning, degradable polymers, synthesis, structure, crosslinking

Affiliations:
Denis P.-IPPT PAN
Wrzecionek M.-other affiliation
Gadomska‐Gajadhur A.-Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)
Sajkiewicz P.-IPPT PAN
2.Kruk A., Gadomska-Gajadhur A., Rykaczewska I., Dulnik J., Ruśkowski P., Synoradzki L., Influence of liquid pore precursors on morphology and mechanical properties of 3D scaffolds obtained by dry inversion phase method, Journal of Biomedical Materials Research Part B: Applied Biomaterials, ISSN: 1552-4973, DOI: 10.1002/jbm.b.34200, Vol.107, No.4, pp.1079-1087, 2019
Abstract:

Polyester 3D scaffolds were obtained by dry inversion phase method. The influence of a polymer and liquid pore precursor type on the 3D scaffolds morphology, porosity and mechanical properties was tested. Polymers and precursors forming a porous structure were identified. It was found that 3D scaffolds having the most preferable structure for cell cultures were obtained from polylactide with the addition of n‐butanol as the liquid pore precursor. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res B Part B, 2018.

Keywords:

liquid pore precursors, mechanical properties, dry inversion phase method, 3D scaffolds

Affiliations:
Kruk A.-Warsaw University of Technology (PL)
Gadomska-Gajadhur A.-Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)
Rykaczewska I.-Warsaw University of Technology (PL)
Dulnik J.-IPPT PAN
Ruśkowski P.-Warsaw University of Technology (PL)
Synoradzki L.-Warsaw University of Technology (PL)
3.Budnicka M., Szymaniak M., Kołbuk D., Ruśkowski P., Gadomska-Gajadhur A., Biomineralization of poly-l-lactide spongy bone scaffolds obtained by freeze-extraction method, Journal of Biomedical Materials Research Part B: Applied Biomaterials, ISSN: 1552-4973, DOI: 10.1002/jbm.b.34441, pp.1-12, 2019
Abstract:

Implants in the form of polymer scaffolds are commonly used to regenerate bone tissue after traumas or tooth extractions. However, few implant formation methods enable building polymer scaffolds allowing to reconstruct larger bone losses without immune response. Spacious, porous poly-l-lactide implants with considerable volume were obtained using the phase inversion method with the freeze-extraction variant. The calcium phosphate (CaP) coating was deposited on implant surfaces with the biomimetic method to improve the implant's osteoconductivity. The substitues morphology was characterized-porosity, size and shape of pores; mechanical properties, mass absorbability of implants before and after mineralization. The characteristics were provided with scanning electron microscopy (SEM), static compression test and hydrostatic weighing, respectively. The presence of CaPs in the entire volume of the implant was confirmed with SEM and infrared spectroscopy with Fourier transform (FTIR). The biocompatibility of scaffolds was confirmed with in vitro quantitative test and microscopic observations. The obtained results show that the implants can be used in tissue engineering as a vehicle of platelet-rich plasma to regenerate critical spongy bone losses.

Keywords:

bone graft, calcium phosphate(s), cell culture

Affiliations:
Budnicka M.-other affiliation
Szymaniak M.-other affiliation
Kołbuk D.-IPPT PAN
Ruśkowski P.-Warsaw University of Technology (PL)
Gadomska-Gajadhur A.-Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)
4.Kruk A., Gadomska-Gajadhur A., Dulnik J., Rykaczewska I., Ruśkowski P., Sebai A., Synoradzki L., Ocena właściwości użytkowych rusztowań komórkowych o strukturze gąbczastej oraz wzrostu na nich fibroblastów, POLIMERY, ISSN: 0032-2725, DOI: 10.14314/polimery.2018.4.3, Vol.63, No.4, pp.18-22, 2018
Abstract:

Zbadano wpływ dodatku ciekłych prekursorów porów na morfologię, porowatość i właściwości mechaniczne polilaktydowych rusztowań komórkowych. Rusztowania otrzymano metodą mokrej inwersji faz w wariancie freeze extraction. Oceniono cytotoksyczność wybranych rusztowań w stosunku do fibroblastów mysich oraz ich przydatność do hodowli komórkowych. Wykazano, że dodatek prekursora porów dopolilaktydu korzystnie zmienia morfologię wytworzonych rusztowań, jednocześnie pogarszając ich wytrzymałość mechaniczną. Stwierdzono, że polilaktydowe rusztowania komórkowe z powodzeniem mogą być wykorzystywane do hodowli komórkowych.

Keywords:

usztowania komórkowe, polilaktyd, hodowle komórkowe, fibroblasty

Affiliations:
Kruk A.-Warsaw University of Technology (PL)
Gadomska-Gajadhur A.-Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)
Dulnik J.-IPPT PAN
Rykaczewska I.-Warsaw University of Technology (PL)
Ruśkowski P.-Warsaw University of Technology (PL)
Sebai A.-Wroclaw University of Science and Technology (PL)
Synoradzki L.-Warsaw University of Technology (PL)

Conference papers
1.Kruk A., Gadomska-Gajadhur A., Ruśkowski P., Chwojnowski A., Dulnik J., Synoradzki L., Preparation of biodegradable semi-permeable membranes as 3D scaffolds for cell cultures, Desalination and Water Treatment, ISSN: 1944-3994, DOI: 10.5004/dwt.2017.11415, Vol.64, pp.317-323, 2017
Abstract:

Results of the preparation of semi-permeable membranes made of biodegradable polymers membranes were presented. Among known polyesters, polylactide was selected for research. The membranes were obtained using wet phase inversion method. The influence of polyvinylpyrrolidone and polymeric nano-non-wovens as pores precursors on the structure of obtained membranes was analysed. It was shown, that utilisation of polymeric nano-non-wovens enabled preparation of semi-permeable membranes, which could be used as wide-pore 3D-type cellular scaffolds.

Keywords:

Biodegradable polymers membranes, Biodegradable polyesters, Porous three-dimensional scaffolds, Inversion phase method

Affiliations:
Kruk A.-Warsaw University of Technology (PL)
Gadomska-Gajadhur A.-Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)
Ruśkowski P.-Warsaw University of Technology (PL)
Chwojnowski A.-Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)
Dulnik J.-IPPT PAN
Synoradzki L.-Warsaw University of Technology (PL)

Conference abstracts
1.Denis P., Sajkiewicz P.Ł., Wrzecionek M., Gadomska-Gajadhur A., Poly(glycerol sebacate) – Poly(L-Lactide) nonwovens. Towards attractive electrospun material for tissue engineering, TERMIS EU 2019, TERMIS European Chapter Meeting 2019, Tissue Engineering Therapies: From Concept to Clinical Translation & Commercialisation, 2019-05-27/05-31, Rodos (GR), pp.1621-1621, 2019
2.Jeznach O., Wrzecionek M., Ruskowski P., Gadomska-Gajdhur A., Kołbuk D., Effect of poly(glycerol succinate) addition on properties of PLA electrospun fibres, ESB2018, 29th European Conference on Biomaterials, 2018-09-09/09-13, Maastricht (NL), pp.1084-1085, 2018
3.Kruk A., Gadomska-Gajadhur A., Ruśkowski P., Chwojnowski A., Dulnik J., Synoradzki L., Preparation of biodegradable semi-permeable membranes as 3D scaffolds for cell cultures, MEMPEP 2016, XI-th Conference on Membranes and Membrane Processes in Environmental Protection, 2016-06-15/06-18, Zakopane (PL), pp.24, 2016

Patents
Filing No./Date
Filing Publication
Autor(s)
Title
Protection Area, Applicant Name
Patent Number
Date of Grant
pdf
430802
2019-08-02
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Gadomska-Gajadhur A., Budnicka M., Ruśkowski P., Kołbuk‐Konieczny D.
Sposób otrzymywania polilaktydowego substytutu kości gąbczastej
PL, Politechnika Warszawska
-
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-
426830
2018-08-28
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Gadomska-Gajadhur A., Ruśkowski P., Synoradzki L., Wrzecionek M., Kołbuk-Konieczny D., Jeznach O., Budnicka M., Szymaniak M.
Sposób otrzymywania poli(bursztynianiu glicerolu)
PL, Politechnika Warszawska
-
-
-
414353
2015-10-13
BUP 09/17
2017-04-24
Chwojnowski A., Łukowska E., Wojciechowski C., Gadomska-Gajadhur A., Kruk A., Ruśkowski P., Synoradzki L., Denis P., Dulnik J., Sajkiewicz P.
Sposób otrzymywania szerokoporowatego, poliestrowego rusztowania komórkowego
PL, Instytut Biocybernetyki i Inżynierii Biomedycznej im. Macieja Nałęcza PAN, Politechnika Warszawska, Instytut Podstawowych Problemów Techniki PAN
228884
WUP 05/18
2018-05-30