Partner: Ł. Buchholz

Nicolaus Copernicus University (PL)

Ostatnie publikacje
1.Pokrywczyńska M., Jundziłł A., Adamowicz J., Kowalczyk T., Warda K., Rasmus M., Buchholz Ł., Krzyżanowska S., Nakielski P., Chmielewski T., Bodnar M., Marszałek A., Dębski R., Frontczak-Baniewicz M.M., Mikułowski G., Nowacki M., Kowalewski T.A., Drewa T., Is the Poly (L- Lactide- Co– Caprolactone) Nanofibrous Membrane Suitable for Urinary Bladder Regeneration?, PLOS ONE, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0105295, Vol.9, No.8, pp.105295-1-12, 2014


The purpose of this study was to compare: a new five-layered poly (L–lactide–co–caprolactone) (PLC) membrane and small intestinal submucosa (SIS) as a control in rat urinary bladder wall regeneration. The five-layered poly (L–lactide–co–caprolactone) membrane was prepared by an electrospinning process. Adipose tissue was harvested from five 8-week old male Wistar rats. Adipose derived stem cells (ADSCs) were seeded in a density of 3×106 cells/cm2 onto PLC membrane and SIS scaffolds, and cultured for 5-7 days in the stem cell culture medium. Twenty male Wistar rats were randomly divided into five equal groups. Augmentation cystoplasty was performed in a previously created dome defect. Groups: (I) PLC+ 3×106ADSCs; (II) SIS+ 3×106ADSCs; (III) PLC; (IV) SIS; (V) control. Cystography was performed after three months. The reconstructed urinary bladders were evaluated in H&E and Masson's trichrome staining. Regeneration of all components of the normal urinary bladder wall was observed in bladders augmented with cell-seeded SIS matrices. The urinary bladders augmented with SIS matrices without cells showed fibrosis and graft contraction. Bladder augmentation with the PLC membrane led to numerous undesirable events including: bladder wall perforation, fistula or diverticula formation, and incorporation of the reconstructed wall into the bladder lumen. The new five-layered poly (L–lactide–co–caprolactone) membrane possesses poorer potential for regenerating the urinary bladder wall compared with SIS scaffold.

Słowa kluczowe:

urinary bladder regeneration, electrospinning

Afiliacje autorów:

Pokrywczyńska M.-other affiliation
Jundziłł A.-other affiliation
Bodnar M.-Nicolaus Copernicus University (PL)
Marszałek A.-Nicolaus Copernicus University (PL)
Dębski R.-Nicolaus Copernicus University (PL)
Frontczak-Baniewicz M.M.-Mossakowski Medical Research Centre, Polish Academy of Sciences (PL)
Mikułowski G.-IPPT PAN
Nowacki M.-other affiliation
Kowalewski T.A.-IPPT PAN
Drewa T.-Nicolaus Copernicus University (PL)
Adamowicz J.-Nicolaus Copernicus University (PL)
Kowalczyk T.-IPPT PAN
Warda K.-other affiliation
Rasmus M.-Nicolaus Copernicus University (PL)
Buchholz Ł.-Nicolaus Copernicus University (PL)
Krzyżanowska S.-other affiliation
Nakielski P.-IPPT PAN
Chmielewski T.-other affiliation