Partner: Jakub Tworkiewicz

Ostatnie publikacje
1.Adamowicz J., Pokrywczyńska M., Tworkiewicz J., Kowalczyk T., van Breda S.V., Tyloch D., Kloskowski T., Bodnar M., Skopińska-Wiśniewska J., Marszałek A., Frontczak-Baniewicz M.M., Kowalewski T.A., Drewa T., New Amniotic Membrane Based Biocomposite for Future Application in Reconstructive Urology, PLOS ONE, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0146012, Vol.11, No.1, pp.e0146012-1-20, 2016


Due to the capacity of the amniotic membrane (Am) to support re-epithelisation and inhibit scar formation, Am has a potential to become a considerable asset for reconstructive urology i.e., reconstruction of ureters and urethrae. The application of Am in reconstructive urology is limited due to a poor mechanical characteristic. Am reinforcement with electrospun nanofibers offers a new strategy to improve Am mechanical resistance, without affecting its unique bioactivity profile. This study evaluated biocomposite material composed of Am and nanofibers as a graft for urinary bladder augmentation in a rat model.

Material and Methods
Sandwich-structured biocomposite material was constructed from frozen Am and covered on both sides with two-layered membranes prepared from electrospun poly-(L-lactide-co-E-caprolactone) (PLCL). Wistar rats underwent hemicystectomy and bladder augmentation with the biocomposite material.

Immunohistohemical analysis (hematoxylin and eosin [H&E], anti-smoothelin and Masson’s trichrome staining [TRI]) revealed effective regeneration of the urothelial and smooth muscle layers. Anti-smoothelin staining confirmed the presence of contractile smooth muscle within a new bladder wall. Sandwich-structured biocomposite graft material was designed to regenerate the urinary bladder wall, fulfilling the requirements for normal bladder tension, contraction, elasticity and compliance. Mechanical evaluation of regenerated bladder wall conducted based on Young’s elastic modulus reflected changes in the histological remodeling of the augmented part of the bladder. The structure of the biocomposite material made it possible to deliver an intact Am to the area for regeneration. An unmodified Am surface supported regeneration of the urinary bladder wall and the PLCL membranes did not disturb the regeneration process.

Am reinforcement with electrospun nanofibers offers a new strategy to improve Am mechanical resistance without affecting its unique bioactivity profile.

Słowa kluczowe:

Bladder, Smooth muscles, Muscle regeneration, Bionanotechnology, Renal system, Urothelium, Urology, Nanomaterials

Afiliacje autorów:

Adamowicz J.-Nicolaus Copernicus University (PL)
Pokrywczyńska M.-other affiliation
Frontczak-Baniewicz M.M.-Mossakowski Medical Research Centre, Polish Academy of Sciences (PL)
Kowalewski T.A.-IPPT PAN
Drewa T.-Nicolaus Copernicus University (PL)
Tworkiewicz J.-other affiliation
Kowalczyk T.-IPPT PAN
van Breda S.V.-University of Pretoria (ZA)
Tyloch D.-other affiliation
Kloskowski T.-other affiliation
Bodnar M.-Nicolaus Copernicus University (PL)
Skopińska-Wiśniewska J.-other affiliation
Marszałek A.-Nicolaus Copernicus University (PL)