Partner: Mirosław Zając

Warsaw University of Life Sciences (PL)

Ostatnie publikacje
1.Skłodowska K., Dębski P.R., Michalski J.A., Korczyk P.M., Dolata M., Zając M., Jakiela S., Simultaneous Measurement of Viscosity and Optical Density of Bacterial Growth and Death in a Microdroplet, Micromachines, ISSN: 2072-666X, DOI: 10.3390/mi9050251, Vol.9, No.5, pp.1-13, 2018

Streszczenie:

Herein, we describe a novel method for the assessment of droplet viscosity moving inside microfluidic channels. The method allows for the monitoring of the rate of the continuous growth of bacterial culture. It is based on the analysis of the hydrodynamic resistance of a droplet that is present in a microfluidic channel, which affects its motion. As a result, we were able to observe and quantify the change in the viscosity of the dispersed phase that is caused by the increasing population of interacting bacteria inside a size-limited system. The technique allows for finding the correlation between the viscosity of the medium with a bacterial culture and its optical density. These features, together with the high precision of the measurement, make our viscometer a promising tool for various experiments in the field of analytical chemistry and microbiology, where the rigorous control of the conditions of the reaction and the monitoring of the size of bacterial culture are vital.

Słowa kluczowe:

droplet microfluidics, cell growth, viscosity, rheology, Escherichia coli

Afiliacje autorów:

Skłodowska K.-Warsaw University of Life Sciences (PL)
Dębski P.R.-Warsaw University of Life Sciences (PL)
Michalski J.A.-Warsaw University of Technology (PL)
Korczyk P.M.-IPPT PAN
Dolata M.-Warsaw University of Life Sciences (PL)
Zając M.-Warsaw University of Life Sciences (PL)
Jakiela S.-Institute of Physical Chemistry, Polish Academy of Sciences (PL)
30p.