dr hab. inż. Wojciech Święszkowski


Promotor prac doktorskich
1.2016-12-09Witecka Agnieszka  
(PW)
Improvement of biocompatibility of magnesium alloys AZ91 and ZM21 by surface modification 

Ostatnie publikacje
1.Górecka Ż., Idaszek J., Kołbuk D., Choińska E., Chlanda A., Święszkowski W., The effect of diameter of fibre on formation of hydrogen bonds and mechanical properties of 3D-printed PCL, Materials Science and Engineering C-Materials for Biological Applications, ISSN: 0928-4931, DOI: 10.1016/j.msec.2020.111072, Vol.114, pp.111072-1-11, 2020

Streszczenie:

Fused Deposition Modelling (FDM) technique has been widely utilized in fabrication of 3D porous scaffolds for tissue engineering (TE) applications. Surprisingly, although there are many publications devoted to the architectural features of the 3D scaffolds fabricated by the FDM, none of them give us evident information about the impact of the diameter of the fibres on material properties. Therefore, the aim of this study was to investigate, for the first time, the effect of the diameter of 3D-printed PCL fibres on variations in their microstructure and resulting mechanical behaviour. The fibres made of poly(ε-caprolactone) (PCL) were extruded through commonly used types of nozzles (inner diameter ranging from 0.18 mm to 1.07 mm) by means of FDM technique. Static tensile test and atomic force microscopy working in force spectroscopy mode revealed strong decrease in the Young's modulus and yield strength with increasing fibre diameter in the investigated range. To explain this phenomenon, we conducted differential scanning calorimetry, wide-angle X-ray-scattering, Fourier-transform infrared spectroscopy, infrared and polarized light microscopy imaging. The obtained results clearly showed that the most prominent effect on the obtained microstructures and mechanical properties had different cooling and shear rates during fabrication process causing changes in supramolecular interactions of PCL. The observed fibre size-dependent formation of hydrogen bonds affected the crystalline structure and its stability. Summarising, this study clearly demonstrates that the diameter of 3D-printed fibres has a strong effect on obtained microstructure and mechanical properties, therefore should be taken into consideration during design of the 3D TE scaffolds.

Słowa kluczowe:

fused deposition modelling, polycaprolactone, mechanical properties, hydrogen bonds, microstructure

Afiliacje autorów:

Górecka Ż.-Warsaw University of Technology (PL)
Idaszek J.-other affiliation
Kołbuk D.-IPPT PAN
Choińska E.-Warsaw University of Technology (PL)
Chlanda A.-Warsaw University of Technology (PL)
Święszkowski W.-IPPT PAN
140p.
2.Rinoldi C., Costantini M., Kijeńska-Gawrońska E., Testa S., Fornetti E., Heljak M., Ćwiklińska M., Buda R., Baldi J., Cannata S., Guzowski J., Gargioli C., Khademhosseini A., Święszkowski W., Tendon tissue engineering: effects of mechanical and biochemical stimulation on stem cell alignment on cell‐laden hydrogel yarns, ADVANCED HEALTHCARE MATERIALS, ISSN: 2192-2659, DOI: 10.1002/adhm.201801218, Vol.8, No.7, pp.1801218-1-10, 2019

Streszczenie:

Fiber-based approaches hold great promise for tendon tissue engineering enabling the possibility of manufacturing aligned hydrogel filaments that can guide collagen fiber orientation, thereby providing a biomimetic micro-environment for cell attachment, orientation, migration, and proliferation. In this study, a 3D system composed of cell-laden, highly aligned hydrogel yarns is designed and obtained via wet spinning in order to reproduce the morphology and structure of tendon fascicles. A bioink composed of alginate and gelatin methacryloyl (GelMA) is optimized for spinning and loaded with human bone morrow mesenchymal stem cells (hBM-MSCs). The produced scaffolds are subjected to mechanical stretching to recapitulate the strains occurring in native tendon tissue. Stem cell differentiation is promoted by addition of bone morphogenetic protein 12 (BMP-12) in the culture medium. The aligned orientation of the fibers combined with mechanical stimulation results in highly preferential longitudinal cell orientation and demonstrates enhanced collagen type I and III expression. Additionally, the combination of biochemical and mechanical stimulations promotes the expression of specific tenogenic markers, signatures of efficient cell differentiation towards tendon. The obtained results suggest that the proposed 3D cell-laden aligned system can be used for engineering of scaffolds for tendon regeneration.

Słowa kluczowe:

hydrogel fibers, static mechanical stretching, stem cell alignment, tenogenic differentiation, wet spinning

Afiliacje autorów:

Rinoldi C.-other affiliation
Costantini M.-Sapienza University of Rome (IT)
Kijeńska-Gawrońska E.-Warsaw University of Technology (PL)
Testa S.-Tor Vergata Rome University (IT)
Fornetti E.-Tor Vergata Rome University (IT)
Heljak M.-Warsaw University of Technology (PL)
Ćwiklińska M.-Institute of Physical Chemistry, Polish Academy of Sciences (PL)
Buda R.-Institute of Physical Chemistry, Polish Academy of Sciences (PL)
Baldi J.-Tor Vergata Rome University (IT)
Cannata S.-Tor Vergata Rome University (IT)
Guzowski J.-Institute of Physical Chemistry, Polish Academy of Sciences (PL)
Gargioli C.-Tor Vergata Rome University (IT)
Khademhosseini A.-Massachusetts Institute of Technology (US)
Święszkowski W.-other affiliation
140p.
3.Rinoldi C., Fallahi A., Yazdi I.K., Paras J.C., Kijeńska-Gawrońska E., Trujillo-de Santiago G., Tuoheti A., Demarchi D., Annabi N., Khademhosseini A., Święszkowski W., Tamayol A., Mechanical and biochemical stimulation of 3D multilayered scaffolds for tendon tissue engineering, ACS BIOMATERIALS SCIENCE & ENGINEERING, ISSN: 2373-9878, DOI: 10.1021/acsbiomaterials.8b01647, Vol.5, No.6, pp.2953-2964, 2019

Streszczenie:

Tendon injuries are frequent and occur in the elderly, young, and athletic populations. The inadequate number of donors combined with many challenges associated with autografts, allografts, xenografts, and prosthetic devices have added to the value of engineering biological substitutes, which can be implanted to repair the damaged tendons. Electrospun scaffolds have the potential to mimic the native tissue structure along with desired mechanical properties and, thus, have attracted noticeable attention. In order to improve the biological responses of these fibrous structures, we designed and fabricated 3D multilayered composite scaffolds, where an electrospun nanofibrous substrate was coated with a thin layer of cell-laden hydrogel. The whole construct composition was optimized to achieve adequate mechanical and physical properties as well as cell viability and proliferation. Mesenchymal stem cells (MSCs) were differentiated by the addition of bone morphogenetic protein 12 (BMP-12). To mimic the natural function of tendons, the cell-laden scaffolds were mechanically stimulated using a custom-built bioreactor. The synergistic effect of mechanical and biochemical stimulation was observed in terms of enhanced cell viability, proliferation, alignment, and tenogenic differentiation. The results suggested that the proposed constructs can be used for engineering functional tendons.

Słowa kluczowe:

tendon tissue engineering, composite scaffolds, nanofibrous materials, mechanical stimulation, stem cell differentiation

Afiliacje autorów:

Rinoldi C.-other affiliation
Fallahi A.-Paul Scherrer Institut (CH)
Yazdi I.K.-Massachusetts Institute of Technology (US)
Paras J.C.-Massachusetts Institute of Technology (US)
Kijeńska-Gawrońska E.-Warsaw University of Technology (PL)
Trujillo-de Santiago G.-Massachusetts Institute of Technology (US)
Tuoheti A.-Politecnico di Torino (IT)
Demarchi D.-Politecnico di Torino (IT)
Annabi N.-Massachusetts Institute of Technology (US)
Khademhosseini A.-Massachusetts Institute of Technology (US)
Święszkowski W.-other affiliation
Tamayol A.-Massachusetts Institute of Technology (US)
140p.
4.Sardelli L., Pacheco D.P., Zorzetto L., Rinoldi C., Święszkowski W., Petrini P., Engineering biological gradients, Journal of Applied Biomaterials & Functional Materials, ISSN: 2280-8000, DOI: 10.1177/2280800019829023, Vol.17, No.1, pp.2280800019829023-1-15, 2019

Streszczenie:

Biological gradients profoundly influence many cellular activities, such as adhesion, migration, and differentiation, which are the key to biological processes, such as inflammation, remodeling, and tissue regeneration. Thus, engineered structures containing bioinspired gradients can not only support a better understanding of these phenomena, but also guide and improve the current limits of regenerative medicine. In this review, we outline the challenges behind the engineering of devices containing chemical-physical and biomolecular gradients, classifying them according to gradient-making methods and the finalities of the systems. Different manufacturing processes can generate gradients in either in-vitro systems or scaffolds, which are suitable tools for the study of cellular behavior and for regenerative medicine; within these, rapid prototyping techniques may have a huge impact on the controlled production of gradients. The parallel need to develop characterization techniques is addressed, underlining advantages and weaknesses in the analysis of both chemical and physical gradients.

Słowa kluczowe:

graded scaffolds, rapid prototyping, bioinspired, microfluidic, gradient characterization, cartilage, bone

Afiliacje autorów:

Sardelli L.-Politecnico di Milano (IT)
Pacheco D.P.-Politecnico di Milano (IT)
Zorzetto L.-University of Liège (BE)
Rinoldi C.-other affiliation
Święszkowski W.-other affiliation
Petrini P.-Politecnico di Milano (IT)
40p.
5.Saghazadeh S., Rinoldi C., Schot M., Kashaf S.S., Sharifi F., Jalilian E., Nuutila K., Giatsidis G., Mostafalu P., Derakhshandeh H., Yue K., Święszkowski W., Memic A., Tamayol A., Khademhosseini A., Drug delivery systems and materials for wound healing applications, Advanced Drug Delivery Reviews, ISSN: 0169-409X, DOI: 10.1016/j.addr.2018.04.008, Vol.127, pp.138-166, 2018

Streszczenie:

Chronic, non-healing wounds place a significant burden on patients and healthcare systems, resulting in impaired mobility, limb amputation, or even death. Chronic wounds result from a disruption in the highly orchestrated cascade of events involved in wound closure. Significant advances in our understanding of the pathophysiology of chronic wounds have resulted in the development of drugs designed to target different aspects of the impaired processes. However, the hostility of the wound environment rich in degradative enzymes and its elevated pH, combined with differences in the time scales of different physiological processes involved in tissue regeneration require the use of effective drug delivery systems. In this review, we will first discuss the pathophysiology of chronic wounds and then the materials used for engineering drug delivery systems. Different passive and active drug delivery systems used in wound care will be reviewed. In addition, the architecture of the delivery platform and its ability to modulate drug delivery are discussed. Emerging technologies and the opportunities for engineering more effective wound care devices are also highlighted.

Słowa kluczowe:

Wound healing, Drug delivery, Transdermal delivery, Microtechnologies, Nanotechnologies

Afiliacje autorów:

Saghazadeh S.-Massachusetts Institute of Technology (US)
Rinoldi C.-other affiliation
Schot M.-Massachusetts Institute of Technology (US)
Kashaf S.S.-Massachusetts Institute of Technology (US)
Sharifi F.-Massachusetts Institute of Technology (US)
Jalilian E.-Massachusetts Institute of Technology (US)
Nuutila K.-Brigham and Women's Hospital (US)
Giatsidis G.-Brigham and Women's Hospital (US)
Mostafalu P.-Massachusetts Institute of Technology (US)
Derakhshandeh H.-University of Nebraska (US)
Yue K.-Massachusetts Institute of Technology (US)
Święszkowski W.-other affiliation
Memic A.-King Abdulaziz University (SA)
Tamayol A.-Massachusetts Institute of Technology (US)
Khademhosseini A.-Massachusetts Institute of Technology (US)
50p.
6.Costantini M., Guzowski J., Żuk P.J., Mozetic P., De Panfilis S., Jaroszewicz J., Heljak M., Massimi M., Pierron M., Trombetta M., Dentini M., Święszkowski W., Rainer A., Garstecki P., Barbetta A., Electric Field Assisted Microfluidic Platform for Generation of Tailorable Porous Microbeads as Cell Carriers for Tissue Engineering, Advanced Functional Materials, ISSN: 1616-301X, DOI: 10.1002/adfm.201800874, Vol.28, pp.1800874-1-13, 2018

Streszczenie:

Injection of cell‐laden scaffolds in the form of mesoscopic particles directly to the site of treatment is one of the most promising approaches to tissue regeneration. Here, a novel and highly efficient method is presented for preparation of porous microbeads of tailorable dimensions (in the range ≈300–1500 mm) and with a uniform and fully interconnected internal porous texture. The method starts with generation of a monodisperse oil‐in‐water emulsion inside a flow‐focusing microfluidic device. This emulsion is later broken‐up, with the use of electric field, into mesoscopic double droplets, that in turn serve as a template for the porous microbeads. By tuning the amplitude and frequency of the electric pulses, the template droplets and the resulting porous bead scaffolds are precisely produced. Furthermore, a model of pulsed electrodripping is proposed that predicts the size of the template droplets as a function of the applied voltage. To prove the potential of the porous microbeads as cell carries, they are tested with human mesenchymal stem cells and hepatic cells, with their viability and degree of microbead colonization being monitored. Finally, the presented porous microbeads are benchmarked against conventional microparticles with nonhomogenous internal texture, revealing their superior performance.

Afiliacje autorów:

Costantini M.-Sapienza University of Rome (IT)
Guzowski J.-Institute of Physical Chemistry, Polish Academy of Sciences (PL)
Żuk P.J.-IPPT PAN
Mozetic P.-Università Campus Bio-Medico di Roma (IT)
De Panfilis S.-Sapienza Istituto Italiano di Tecnologia (IT)
Jaroszewicz J.-other affiliation
Heljak M.-Warsaw University of Technology (PL)
Massimi M.-University of L’Aquila (IT)
Pierron M.-Telecom Physique Strasbourg (FR)
Trombetta M.-Università Campus Bio-Medico di Roma (IT)
Dentini M.-Sapienza University of Rome (IT)
Święszkowski W.-other affiliation
Rainer A.-Università Campus Bio-Medico di Roma (IT)
Garstecki P.-Institute of Physical Chemistry, Polish Academy of Sciences (PL)
Barbetta A.-Sapienza University of Rome (IT)
45p.
7.Sajkiewicz P., Heljak M.K., Gradys A., Choińska E., Rumiński S., Jaroszewicz T., Bissenik I., Święszkowski W., Degradation and related changes in supermolecular structure of poly(caprolactone) in vivo conditions, Polymer Degradation and Stability, ISSN: 0141-3910, DOI: 10.1016/j.polymdegradstab.2018.09.023, Vol.157, pp.70-79, 2018

Streszczenie:

The degradation in vivo and its effect on the supermolecular structure of poly(caprolactone) was examined. Poly(caprolactone) (PCL) samples were prepared in the form of porous scaffolds implanted into rat calvarial defects. The degradation was investigated by means of gel permeation chromatography, wide angle X-ray scattering (WAXS), scanning electron microscopy (SEM) and differential scanning calorimetry (DSC). The study showed that the observed decrease of PCL crystallinity during degradation is accompanied by reduction of crystal size and/or perfection. The observed phenomenon could be explained by the presence of the high content of the low mobile fraction of investigated polymer, consisting not only almost 50% of crystal fraction but also most probably relatively high fraction of s.c. rigid amorphous fraction (RAF). Considering the type of structure characterized by the dominance of low mobile fraction, it is expected that the degradation will mainly concern these fractions, which in turn will lead to a decrease in the degree of crystallinity as well as crystal size and/or perfection.

Słowa kluczowe:

PCL degradation, In-vivo conditions, Crystallinity, Rigid amorphous fraction

Afiliacje autorów:

Sajkiewicz P.-IPPT PAN
Heljak M.K.-Warsaw University of Technology (PL)
Gradys A.-IPPT PAN
Choińska E.-Warsaw University of Technology (PL)
Rumiński S.-Medical University of Warsaw (PL)
Jaroszewicz T.-Warsaw University of Technology (PL)
Bissenik I.-Warsaw University of Life Sciences (PL)
Święszkowski W.-other affiliation
35p.
8.Urbanek O., Pierini F., Choińska E., Sajkiewicz P., Bil M., Święszkowski W., Effect of hydroxyapatite nanoparticles addition on structure properties of poly(L-lactide-co-glycolide) after gamma sterilization, Polymer Composites, ISSN: 0272-8397, DOI: 10.1002/pc.24028, Vol.39, No.4, pp.1023-1031, 2018

Streszczenie:

Physical and chemical factors resulting from the sterilization methods may affect the structure and properties of the materials which undergo this procedure. Poly(l-lactide-co-glicolide) (PLGA) is commonly used for medical applications, but, due to its inadequate mechanical properties, it is not recommended for load-bearing applications. One of the methods for improving PLGA mechanical properties is addition of hydroxyapatite nanoparticles (nHAp). The aim of this study was to evaluate the effect of nanoparticles addition on PLGA structure and properties after gamma radiation. According to our results, reduction of the molecular mass caused by gamma radiation was lower for PLGA with nHAp addition. Differential scanning calorimetry (DSC) analysis indicates an increase of crystallinity caused both by nHAp and gamma radiation. The first phenomenon can be explained by heteronucleation, while the second one is most probably related to higher molecular mobility of degrading polymer. Moreover, addition of nanoparticles increases thermal stability and affects the Young's modulus changes after gamma radiation.

Afiliacje autorów:

Urbanek O.-IPPT PAN
Pierini F.-IPPT PAN
Choińska E.-Warsaw University of Technology (PL)
Sajkiewicz P.-IPPT PAN
Bil M.-Warsaw University of Technology (PL)
Święszkowski W.-other affiliation
30p.
9.Chlanda A., Kijeńska E., Rinoldi C., Tarnowski M., Wierzchoń T., Święszkowski W., Structure and physico-mechanical properties of low temperature plasma treated electrospun nanofibrous scaffolds examined with atomic force microscopy, Micron, ISSN: 0968-4328, DOI: 10.1016/j.micron.2018.01.012, Vol.107, pp.79-84, 2018

Streszczenie:

Electrospun nanofibrous scaffolds are willingly used in tissue engineering applications due to their tunable mechanical, chemical and physical properties. Additionally, their complex openworked architecture is similar to the native extracellular matrix of living tissue. After implantation such scaffolds should provide sufficient mechanical support for cells. Moreover, it is of crucial importance to ensure sterility and hydrophilicity of the scaffold. For this purpose, a low temperature surface plasma treatment can be applied. In this paper, we report physico-mechanical evaluation of stiffness and adhesive properties of electrospun mats after their exposition to low temperature plasma. Complex morphological and mechanical studies performed with an atomic force microscope were followed by scanning electron microscope imaging and a wettability assessment. The results suggest that plasma treatment can be a useful method for the modification of the surface of polymeric scaffolds in a desirable manner. Plasma treatment improves wettability of the polymeric mats without changing their morphology.

Słowa kluczowe:

Atomic force microscopy, Surface modification, Electrospun fibers, RF plasma treatment, Tissue engineering, Nanomaterial

Afiliacje autorów:

Chlanda A.-Warsaw University of Technology (PL)
Kijeńska E.-Warsaw University of Technology (PL)
Rinoldi C.-other affiliation
Tarnowski M.-Warsaw University of Technology (PL)
Wierzchoń T.-Warsaw University of Technology (PL)
Święszkowski W.-other affiliation
30p.
10.Rinoldi C., Kijeńska E., Chlanda A., Choińska E., Khenoussi N., Tamayol A., Khademhosseini A., Święszkowski W., Nanobead-on-string composites for tendon tissue engineering, JOURNAL OF MATERIALS CHEMISTRY B , ISSN: 2050-7518, DOI: 10.1039/c8tb00246k, Vol.6, No.19, pp.3116-3127, 2018

Streszczenie:

Tissue engineering holds great potential in the production of functional substitutes to restore, maintain or improve the functionality in defective or lost tissues. So far, a great variety of techniques and approaches for fabrication of scaffolds have been developed and evaluated, allowing researchers to tailor precisely the morphological, chemical and mechanical features of the final constructs. Electrospinning of biocompatible and biodegradable polymers is a popular method for producing homogeneous nanofibrous structures, which might reproduce the nanosized organization of the tendons. Moreover, composite scaffolds obtained by incorporating nanoparticles within electrospun fibers have been lately explored in order to enhance the properties and the functionalities of the pristine polymeric constructs. The present study is focused on the design and fabrication of biocompatible electrospun nanocomposite fibrous scaffolds for tendon regeneration. A mixture of poly(amide 6) and poly(caprolactone) is electrospun to generate constructs with mechanical properties comparable to that of native tendons. To improve the biological activity of the constructs and modify their topography, wettability, stiffness and degradation rate, we incorporated silica particles into the electrospun substrates. The use of nanosize silica particles enables us to form bead-on-fiber topography, allowing the better exposure of ceramic particles to better profit their beneficial characteristics. In vitro biocompatibility studies using L929 fibroblasts demonstrated that the presence of 20 wt% of silica nanoparticles in the engineered scaffolds enhanced cell spreading and proliferation as well as extracellular matrix deposition. The results reveal that the electrospun nanocomposite scaffold represents an interesting candidate for tendon tissue engineering.

Afiliacje autorów:

Rinoldi C.-other affiliation
Kijeńska E.-Warsaw University of Technology (PL)
Chlanda A.-Warsaw University of Technology (PL)
Choińska E.-Warsaw University of Technology (PL)
Khenoussi N.-Université de Haute Alsace (FR)
Tamayol A.-Massachusetts Institute of Technology (US)
Khademhosseini A.-Massachusetts Institute of Technology (US)
Święszkowski W.-other affiliation
11.Nasajpour A., Ansari S., Rinoldi C., Rad A.S., Aghaloo T., Shin S.R., Mishra Y.K., Adelung R., Święszkowski W., Annabi N., Khademhosseini A., Moshaverinia A., Tamayol A., A Multifunctional Polymeric Periodontal Membrane with Osteogenic and Antibacterial Characteristics, Advanced Functional Materials, ISSN: 1616-301X, DOI: 10.1002/adfm.201703437, Vol.28, No.3, pp.1703437-1-8, 2017

Streszczenie:

Periodontitis is a prevalent chronic, destructive inflammatory disease affecting tooth‐supporting tissues in humans. Guided tissue regeneration strategies are widely utilized for periodontal tissue regeneration generally by using a periodontal membrane. The main role of these membranes is to establish a mechanical barrier that prevents the apical migration of the gingival epithelium and hence allowing the growth of periodontal ligament and bone tissue to selectively repopulate the root surface. Currently available membranes have limited bioactivity and regeneration potential. To address such challenges, an osteoconductive, antibacterial, and flexible poly(caprolactone) (PCL) composite membrane containing zinc oxide (ZnO) nanoparticles is developed. The membranes are fabricated through electrospinning of PCL and ZnO particles. The physical properties, mechanical characteristics, and in vitro degradation of the engineered membrane are studied in detail. Also, the osteoconductivity and antibacterial properties of the developed membrane are analyzed in vitro. Moreover, the functionality of the membrane is evaluated with a rat periodontal defect model. The results confirmed that the engineered membrane exerts both osteoconductive and antibacterial properties, demonstrating its great potential for periodontal tissue engineering.

Słowa kluczowe:

electrospinning, guided tissue regeneration, osteoconductive, periodontal regeneration, zinc oxide

Afiliacje autorów:

Nasajpour A.-Massachusetts Institute of Technology (US)
Ansari S.-University of California (US)
Rinoldi C.-other affiliation
Rad A.S.-Massachusetts Institute of Technology (US)
Aghaloo T.-University of California (US)
Shin S.R.-Massachusetts Institute of Technology (US)
Mishra Y.K.-Kiel University (DE)
Adelung R.-Kiel University (DE)
Święszkowski W.-other affiliation
Annabi N.-Massachusetts Institute of Technology (US)
Khademhosseini A.-Massachusetts Institute of Technology (US)
Moshaverinia A.-University of California (US)
Tamayol A.-Massachusetts Institute of Technology (US)
45p.
12.Witecka A., Yamamoto A., Święszkowski W., Influence of SaOS-2 cells on corrosion behavior of cast Mg-2.0Zn0.98Mn magnesium alloy, COLLOIDS AND SURFACES B-BIOINTERFACES, ISSN: 0927-7765, DOI: 10.1016/j.colsurfb.2016.10.041, Vol.150, pp.288-296, 2017

Streszczenie:

In this research, the effect of the presence of living cells (SaOS-2) on in vitro degradation of Mg-2.0Zn-0.98Mn (ZM21) magnesium alloy was examined by two methods simple immersion/cell culture tests and electrochemical measurements (electrochemical impedance spectroscopy and potentiodynamic polarization) under cell culture conditions. In immersion/cell culture tests, when SaOS-2 cells were cultured on ZM21 samples, pH of cell culture medium decreased, therefore weight loss and Mg2+ ion release from the samples increased. Electrochemical measurements revealed the presence of living cells increased corrosion rate (Icorr) and decreased polarization resistance (Rp) after 48 h of incubation. This acceleration of ZM21 corrosion can mainly be attributed to the decrease of medium pH due to cellular metabolic activities.

Słowa kluczowe:

biodegradable metals, biomaterials, electrochemical impedance spectroscopy, immersion, cell culture condition

Afiliacje autorów:

Witecka A.-other affiliation
Yamamoto A.-National Institute for Materials Science (JP)
Święszkowski W.-other affiliation
35p.
13.Celikkin N., Rinoldi C., Costantini M., Trombetta M., Rainer A., Święszkowski W., Naturally derived proteins and glycosaminoglycan scaffolds for tissue engineering applications, Materials Science and Engineering C-Materials for Biological Applications, ISSN: 0928-4931, DOI: 10.1016/j.msec.2017.04.016, Vol.78, pp.1277-1299, 2017

Streszczenie:

Tissue engineering (TE) aims to mimic the complex environment where organogenesis takes place using advanced materials to recapitulate the tissue niche. Cells, three-dimensional scaffolds and signaling factors are the three main and essential components of TE. Over the years, materials and processes have become more and more sophisticated, allowing researchers to precisely tailor the final chemical, mechanical, structural and biological features of the designed scaffolds. In this review, we will pose the attention on two specific classes of naturally derived polymers: fibrous proteins and glycosaminoglycans (GAGs). These materials hold great promise for advances in the field of regenerative medicine as i) they generally undergo a fast remodeling in vivo favoring neovascularization and functional cells organization and ii) they elicit a negligible immune reaction preventing severe inflammatory response, both representing critical requirements for a successful integration of engineered scaffolds with the host tissue. We will discuss the recent achievements attained in the field of regenerative medicine by using proteins and GAGs, their merits and disadvantages and the ongoing challenges to move the current concepts to practical clinical application.

Słowa kluczowe:

Natural polymers, Hydrogel scaffolds, Glycosaminoglycans (GAGs), Fibrous proteins, Regenerative medicine

Afiliacje autorów:

Celikkin N.-Warsaw University of Technology (PL)
Rinoldi C.-other affiliation
Costantini M.-Sapienza University of Rome (IT)
Trombetta M.-Università Campus Bio-Medico di Roma (IT)
Rainer A.-Università Campus Bio-Medico di Roma (IT)
Święszkowski W.-other affiliation
30p.
14.Witecka A., Yamamoto A., Idaszek J., Chlanda A., Święszkowski W., Influence of biodegradable polymer coatings on corrosion, cytocompatibility and cell functionality of Mg-2.0Zn-0.98Mn magnesium alloy, COLLOIDS AND SURFACES B-BIOINTERFACES, ISSN: 0927-7765, DOI: 10.1016/j.colsurfb.2016.04.021, Vol.144, pp.284-292, 2016

Streszczenie:

Four kinds of biodegradable polymers were employed to prepare bioresorbable coatings on Mg-2.0Zn-0.98Mn (ZM21) alloy to understand the relationship between polymer characteristics, protective effects on substrate corrosion, cytocompatibility and cell functionality. Poly-l-lactide (PLLA), poly(3-hydroxybutyrate) (PHB), poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) or poly(lactic-co-glycolic) acid (PLGA) was spin-coated on ZM21, obtaining a smooth, non-porous coating less than 0.5 μm in thickness. Polymer coating characterization, a degradation study, and biocompatibility evaluations were performed. After 4 w of immersion into cell culture medium, degradation of PLGA and PLLA coatings were confirmed by ATR-FTIR observation. The coatings of PLLA, PHB and PHBV, which have lower water permeability and slower degradation than PLGA, provide better suppression of initial ZM21 degradation and faster promotion of human osteosarcoma cell growth and differentiation.

Słowa kluczowe:

Biodegradable metal, Magnesium alloy, Biodegradable polymer, SaOS-2 differentiation, Calcification

Afiliacje autorów:

Witecka A.-other affiliation
Yamamoto A.-National Institute for Materials Science (JP)
Idaszek J.-other affiliation
Chlanda A.-Warsaw University of Technology (PL)
Święszkowski W.-other affiliation
35p.
15.Witecka A., Bogucka A., Yamamoto A., Máthis K., Krajňák T., Jaroszewicz J., Święszkowski W., In vitro degradation of ZM21 magnesium alloy in simulated body fluids, Materials Science and Engineering C-Materials for Biological Applications, ISSN: 0928-4931, DOI: 10.1016/j.msec.2016.04.019, Vol.65, pp.59-69, 2016

Streszczenie:

In vitro degradation behavior of squeeze cast (CAST) and equal channel angular pressed (ECAP) ZM21 magnesium alloy (2.0 wt% Zn-0.98 wt% Mn) was studied using immersion tests up to 4 w in three different biological environments. Hanks' Balanced Salt Solution (Hanks), Earle's Balanced Salt Solution (Earle) and Eagle minimum essential medium supplemented with 10% (v/v) fetal bovine serum (E-MEM + 10% FBS) were used to investigate the effect of carbonate buffer system, organic compounds and material processing on the degradation behavior of the ZM21 alloy samples. Corrosion rate of the samples was evaluated by their Mg2 + ion release, weight loss and volume loss. In the first 24 h, the corrosion rate sequence of the CAST samples was as following: Hanks > E-MEM + 10% FBS > Earle. However, in longer immersion periods, the corrosion rate sequence was Earle > E-MEM + 10% FBS ≥ Hanks. Strong buffering effect provided by carbonate buffer system helped to maintain the pH avoiding drastic increase of the corrosion rate of ZM21 in the initial stage of immersion. Organic compounds also contributed to maintain the pH of the fluid. Moreover, they adsorbed on the sample surface and formed an additional barrier on the insoluble salt layer, which was effective to retard the corrosion of CAST samples. In case of ECAP, however, this effect was overcome by the occurrence of strong localized corrosion due to the lower pH of the medium. Corrosion of ECAP samples was much greater than that of CAST, especially in Hanks, due to higher sensitivity of ECAP to localized corrosion and the presence of Cl−.

The present work demonstrates the importance of using an appropriate solution for a reliable estimation of the degradation rate of Mg-base degradable implants in biological environments, and concludes that the most appropriate solution for this purpose is E-MEM + 10% FBS, which has the closest chemical composition to human blood plasma.

Słowa kluczowe:

ZM21 magnesium alloy, ECAP, Simulated body fluids, In vitro degradationBicomponent nanofibers, Biodegradation, Biopolymer

Afiliacje autorów:

Witecka A.-other affiliation
Bogucka A.-Warsaw University of Technology (PL)
Yamamoto A.-National Institute for Materials Science (JP)
Máthis K.-Charles University in Prague (CZ)
Krajňák T.-Charles University in Prague (CZ)
Jaroszewicz J.-other affiliation
Święszkowski W.-other affiliation
30p.
16.Enayati M.S., Behzad T., Sajkiewicz P., Bagheri R., Ghasemi‑Mobarakeh L., Kuśnieruk S., Rogowska-Tylman J., Pahlevanneshan Z., Choińska E., Święszkowski W., Fabrication and characterization of electrospun bionanocomposites of poly (vinyl alcohol)/ nanohydroxyapatite/cellulose nanofibers, International Journal of Polymeric Materials and Polymeric Biomaterials, ISSN: 0091-4037, DOI: 10.1080/00914037.2016.1157798, Vol.65, No.13, pp.660-674, 2016

Streszczenie:

The aim of the present study was preparation, optimization, and systematic characterization of electrospun bionanocomposite fibers based on polyvinyl alcohol (PVA) as matrix and nanohydroxy apatite (nHAp) and cellulose nanofibers (CNF) as nanoreinforcements. The presence of nHAp and nHAp-CNF affected the morphology of electrospun mats and reduced fiber diameter, particularly at a higher content of nanofillers. The obtained results of FTIR, DSC, and WAXS proved the crystallinity reduction of electrospun nancomposites. Both nHAp and nHAp-CNF addition led to a significant increase of Young modulus with the highest stiffness for nanocomposite fibers at 10 wt% of nHAp and 3 wt% of CNF.

Słowa kluczowe:

Bionanocomposite, cellulose nanofibers, electrospinning, nanohydroxy apatite

Afiliacje autorów:

Enayati M.S.-Isfahan University of Technology (IR)
Behzad T.-Isfahan University of Technology (IR)
Sajkiewicz P.-IPPT PAN
Bagheri R.-Isfahan University of Technology (IR)
Ghasemi‑Mobarakeh L.-Isfahan University of Technology (IR)
Kuśnieruk S.-other affiliation
Rogowska-Tylman J.-other affiliation
Pahlevanneshan Z.-Payame Noor University (IR)
Choińska E.-Warsaw University of Technology (PL)
Święszkowski W.-other affiliation
20p.
17.Witecka A., Yamamoto A., Święszkowski W., Influence of SaOS-2 cells on corrosion behaviour of cast ZM21 magnesium alloy, European Cells and Materials, ISSN: 1473-2262, Vol.28, No.Suppl. 3, pp.71, 201440p.
18.Figurska M., Święszkowski W., Telega J.J., Influence of wear debris on behaviour and biomechanical properties of bone-implant interface, Russian Journal of Biomechanics, ISSN: 1812-5123, Vol.9, No.2, pp.20-32, 2005

Streszczenie:

The aim of this paper is to describe the effects of wear particles, generated during articulation of the bearing surfaces of the total joint prosthesis on bone-implant interface. Submicron particles migrate into effective joint space and stimulate cells present in the fibrous tissue to release molecular signal. Cytokines activate the osteoclasts and in consequence bone loss may result. It weakens the bone-implant fixation and may cause aseptic loosening of the prosthesis.

Słowa kluczowe:

wear debris, bone–implant interface, total joint replacemtnt

Afiliacje autorów:

Figurska M.-IPPT PAN
Święszkowski W.-other affiliation
Telega J.J.-IPPT PAN

Lista rozdziałów w ostatnich monografiach
1.
625
Costantini M., Testa S., Rinoldi C., Celikkin N., Idaszek J., Colosi C., Gargioli C., Święszkowski W., Barbetta A., Biomaterials Science Series, Biofabrication and 3D Tissue Modeling, rozdział: 3D Tissue Modelling of Skeletal Muscle Tissue, Royal Society of Chemistry, Edited by Dong-Woo Cho, 3, pp.184-215, 2019
2.
134
Święszkowski W., Figurska M., Berse H.E.N., Kurzydłowski K.J., Biomaterials in the orthopaedic practice, ABIOMED Lecture Notes 5, rozdział: In vivo degradation and wear of biomaterials in total joint replacements, IPPT PAN, ABIOMED (Warszawa), Lekszycki T., Małdyk P. (Eds.), 5, pp.97-115, 2005

Prace konferencyjne
1.Witecka A., Yamamoto A., Święszkowski W., Improvement of cytocompatibility of magnesium alloy zm21 by surface modification, TMS 2014, The Magnesium Technology Symposium, 2014-02-16/02-20, San Diego (US), DOI: 10.1002/9781118888179.ch71, pp.375-380, 2014

Streszczenie:

Application of a biodegradable polymer coating is one of the methods to improve the initial corrosion resistance and cytocompatibility of magnesium (Mg) alloys. However, bulging of the coating film during long term immersion has been reported. Therefore, improvement of interface strength between the coating and the substrate surface is a key for the success of this method. Combination of surface modification [silanization with 3- (glycidyloxypropyl) triethoxysilane (GPTES)] and biodegradable polymer coating [poly-L-lactide (PLLA)] were applied to a Mg- 2.0Zn-0.98Mn (ZM21) cast alloy. Results of a cell proliferation assay show that PLLA and GPTES+PLLA coating successfully improved cell growth during 7 days of incubation and suppressed Mg2+ release after 4 days of incubation. The silanization process had no impact on suppression of corrosion. Calcification was observed on all samples after 1 week of incubation with calcification medium, but the calcified area was much larger on the GPTES+PLLA coated sample than on the uncoated sample.

Słowa kluczowe:

biodegradable metal, ZM21, cytocompatibility, silane-coupling, PLLA

Afiliacje autorów:

Witecka A.-other affiliation
Yamamoto A.-National Institute for Materials Science (JP)
Święszkowski W.-IPPT PAN
2.Secomski W., Nowicki A., Święszkowski W., Controlled ultrasonic destruction of the polycaprolactone shell microcapsules based on resonance scattering theory, 10th Polish - Japanese Seminar on Biomedical Engineering - "New Trends in Biomedical and Clinical Engineering", 2009-09-14/09-16, Warszawa (PL), pp.80-84, 2010

Streszczenie:

The use of the ultrasonically destructible microcapsules as local drug delivery systems continues to grow. Microbubble destruction requires correct ultrasonic frequency equal to its resonance. This frequency depends on the bubble size and polymer shell stiffness. Measurements of the ultrasonic signal, backscattered from microspheres gives practical information of the bubble resonance and nonlinearity.
In experiment, the backscattered power spectrum of measured sample was recorded by an ultrasonic scanner. Radio frequency (RF) data was recorded at 2.0 – 6.6 MHz. The mean particle diameter in the measured sample was 21 μm. The resonance frequency, measured under the microscope, was 0.60 MHz for 43 μm diameter microsphere. The sample volume was 10cm³ and the mean quantity of scatterers was 6•103/cm³.
The simulated power spectrum of the ultrasonic backscattered signal was calculated from the resonance scattering theory for the gas bubbles surrounded by elastic shell.
In conclusion, the measured spectra matched those calculated from the theory. The use of the ultrasonic scanner with RF data output and the high sensitivity, wide bandwidth ultrasonic transducer allows to measure the backscattered signal from the very small quantity of resonance scatterers with satisfactory results at 40 dB signal to noise ratio.

Słowa kluczowe:

ultrasound, microcapsules, controlled drug delivery, backscattering

Afiliacje autorów:

Secomski W.-IPPT PAN
Nowicki A.-IPPT PAN
Święszkowski W.-other affiliation

Abstrakty konferencyjne
1.Witecka A., Yamamoto A., Święszkowski W., Basista M., Influence of polymer film concentration on cytocompatibility and corrosion suppression of ZM21 magnesium alloy, 7th KMM-VIN Industrial Workshop: Biomaterials: Key Technologies for Better Healthcare, 2017-09-27/09-28, Erlangen (DE), pp.19-19, 2017
2.Kukla D., Staszczak M., Pieczyska E.A., Heljak M., Szlązak K., Święszkowski W., Cristea M., Tobushi H., Hayashi S., Evaluation of the properties of polymeric foams with shape memory under load, PCM-CMM 2015, 3rd Polish Congress of Mechanics and 21st Computer Methods in Mechanics, 2015-09-08/09-11, Gdańsk (PL), pp.143-144, 2015

Streszczenie:

The paper presents the results of experimental investigation on polymer foam with shape memory properties. The research is focused on characterization of the microstructure of the foam and understanding the mechanisms of deformation under static and dynamic loading. Up till now, selected experimental techniques have been applied. Dynamic Mechanical Analysis (DMA) allows determining the extent of the value of the glass transition temperature under different load conditions, which also reveals the transformation temperature range for the SMP foam. Scanning electron microscopy (SEM) shows the foam microstructure in various scales, while X-ray tomography gave 3D microstructure results presenting in addition mechanism of the cells deformation and changes in their geometry under 30 % and 50% strain. BOSE system enables obtaining the results on dynamic loading.

Słowa kluczowe:

Shape memory polymer foam, Dynamic mechanical analysis, Glass transition temperature, X-ray tomography

Afiliacje autorów:

Kukla D.-IPPT PAN
Staszczak M.-IPPT PAN
Pieczyska E.A.-IPPT PAN
Heljak M.-Warsaw University of Technology (PL)
Szlązak K.-other affiliation
Święszkowski W.-other affiliation
Cristea M.-Petru Poni Institute of Macromolecular Chemistry (RO)
Tobushi H.-Aichi Institute of Technology (JP)
Hayashi S.-SMP Technologies Inc. (JP)
3.Urbanek O., Bil M., Święszkowski W., The effect of hydroxyapatite nanoparticles addition on crystallinity and surface properties of bioresorbable nanocomposites after gamma sterylization treatment, 4th Summer Symposium on Nanomaterials and Their Application to Biology and Medicine, 2014-06-15/06-18, Poznań (PL), pp.1, 2014

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pdf
423442
2017-11-13
BUP 11/19
2019-05-20
Wysocki B., Żrodowski Ł., Chmielewska A., Święszkowski W., Supeł A., Kurzydłowski K., Czarnecka K.
Sposób wytwarzania addytywnego trójwymiarowych obiektów
PL, Politechnika Warszawska
233190
WUP 09/19
2019-09-30