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Recent publications
1.  Białobrzeska W., Firganek D., Czerkies M., Lipniacki T., Skwarecka M., Dziąbowska K., Cebula Z., Malinowska N., Bigus D., Bięga E., Pyrć K., Pala K., Żołędowska S., Nidzworski D., Electrochemical immunosensors based on screen-printed gold and glassy carbon electrodes: comparison of performance for respiratory syncytial virus detection, Biosensors, ISSN: 2079-6374, DOI: 10.3390/bios10110175, Vol.10, No.11, pp.175-1-13, 2020

Abstract:
This paper presents the development and comparison of label-free electrochemical immunosensors based on screen-printed gold and glassy carbon (GC) disc electrodes for efficient and rapid detection of respiratory syncytial virus (RSV). Briefly, the antibody specific to the F protein of RSV was successfully immobilized on modified electrodes. Antibody coupling on the Au surface was conducted via 4-aminothiophenol (4-ATP) and glutaraldehyde (GA). The GC surface was modified with poly-L-lysine (PLL) for direct anti-RSV conjugation after EDC/NHS (1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-Hydroxysuccinimide) activation. Electrochemical characterizations of the immunosensors were carried out by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). GC-based immunosensors show a dynamic range of antigen detection from 1.0 × 10^5 PFU/mL to 1.5×10^7 PFU/mL, more than 1.0 × 10^5 PFU/mL to 1.0 × 10^7 PFU/mL for the Au-based sensor. However, the GC platform is less sensitive and shows a higher detection limit (LOD) for RSV. The limit of detection of the Au immunosensor is 1.1 × 10^3 PFU/mL, three orders of magnitude lower than 2.85 × 10^6 PFU/mL for GC. Thus, the Au-based immunosensor has better analytical performance for virus detection than a carbon-based platform due to high sensitivity and very low RSV detection, obtained with good reproducibility.

Keywords:
respiratory syncytial virus, cyclic voltammetry, electrochemical impedance spectroscopy, sensor, gold electrode, glassy carbon

Affiliations:
Białobrzeska W. - other affiliation
Firganek D. - other affiliation
Czerkies M. - IPPT PAN
Lipniacki T. - IPPT PAN
Skwarecka M. - other affiliation
Dziąbowska K. - other affiliation
Cebula Z. - other affiliation
Malinowska N. - other affiliation
Bigus D. - other affiliation
Bięga E. - other affiliation
Pyrć K. - other affiliation
Pala K. - other affiliation
Żołędowska S. - other affiliation
Nidzworski D. - other affiliation

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