Sylwia Pawłowska, M.Sc., Eng.

Department of Biosystems and Soft Matter (ZBiMM)
Division of Modelling in Biology and Medicine (PMBM)
position: lab technician
telephone: (+48) 22 826 12 81 ext.: 330
room: 320
e-mail: spaw

Doctoral thesis
2018-12-11Dynamics of nano objects suspended in liquids: experimental analysis  (IBIB PAN)
supervisor -- Prof. Tomasz Kowalewski, Ph.D., Dr. Habil., IPPT PAN
supervisor -- Prof. Dr. Filippo Pierini, IPPT PAN
1262 
Recent publications
1.Pierini F., Nakielski P., Urbanek O., Pawłowska S., Lanzi M., De Sio L., Kowalewski T.A., Polymer-Based Nanomaterials for Photothermal Therapy: From Light-Responsive to Multifunctional Nanoplatforms for Synergistically Combined Technologies, BIOMACROMOLECULES, ISSN: 1525-7797, DOI: 10.1021/acs.biomac.8b01138, Vol.19, No.11, pp.4147-4167, 2018
Abstract:

Materials for the treatment of cancer have been studied comprehensively over the past few decades. Among the various kinds of biomaterials, polymer-based nanomaterials represent one of the most interesting research directions in nanomedicine because their controlled synthesis and tailored designs make it possible to obtain nanostructures with biomimetic features and outstanding biocompatibility. Understanding the chemical and physical mechanisms behind the cascading stimuli-responsiveness of smart polymers is fundamental for the design of multifunctional nanomaterials to be used as photothermal agents for targeted polytherapy. In this review, we offer an in-depth overview of the recent advances in polymer nanomaterials for photothermal therapy, describing the features of three different types of polymer-based nanomaterials. In each case, we systematically show the relevant benefits, highlighting the strategies for developing light-controlled multifunctional nanoplatforms that are responsive in a cascade manner and addressing the open issues by means of an inclusive state-of-the-art review. Moreover, we face further challenges and provide new perspectives for future strategies for developing novel polymeric nanomaterials for photothermally assisted therapies.

Affiliations:
Pierini F.-IPPT PAN
Nakielski P.-IPPT PAN
Urbanek O.-IPPT PAN
Pawłowska S.-IPPT PAN
Lanzi M.-University of Bologna (IT)
De Sio L.-Sapienza University of Rome (IT)
Kowalewski T.A.-IPPT PAN
2.Pawłowska S., Kowalewski T.A., Pierini F., Fibrous polymer nanomaterials for biomedical applications and their transport by fluids: an overview, SOFT MATTER, ISSN: 1744-683X, DOI: 10.1039/C8SM01269E, Vol.14, pp.8421-8444, 2018
Abstract:

Over the past few decades, there has been strong interest in the development of new micro- and nanomaterials for biomedical applications. Their use in the form of capsules, particles or filaments suspended in body fluids is associated with conformational changes and hydrodynamic interactions responsible for their transport. The dynamics of fibres or other long objects in Poiseuille flow is one of the fundamental problems in a variety of biomedical contexts, such as mobility of proteins, dynamics of DNA or other biological polymers, cell movement, tissue engineering, and drug delivery. In this review, we discuss several important applications of micro and nanoobjects in this field and try to understand the problems of their transport in flow resulting from material-environment interactions in typical, crowded, and complex biological fluids. Our aim is to elucidate the relationship between the nano- and microscopic structures of elongated polymer particles and their flow properties, thus opening the possibility to design nanoobjects that can be efficiently transported by body fluids for targeted drug release or local tissue regeneration.

Affiliations:
Pawłowska S.-IPPT PAN
Kowalewski T.A.-IPPT PAN
Pierini F.-IPPT PAN
3.Pierini F., Lanzi M., Nakielski P., Pawłowska S., Urbanek O., Zembrzycki K., Kowalewski T.A., Single-Material Organic Solar Cells Based on Electrospun Fullerene-Grafted Polythiophene Nanofibers, Macromolecules, ISSN: 0024-9297, DOI: 10.1021/acs.macromol.7b00857, Vol.50, No.13, pp.4972-4981, 2017
Abstract:

Highly efficient single-material organic solar cells (SMOCs) based on fullerene-grafted polythiophenes were fabricated by incorporating electrospun one-dimensional (1D) nanostructures obtained from polymer chain stretching. Poly(3-alkylthiophene) chains were chemically tailored in order to reduce the side effects of charge recombination which severely affected SMOC photovoltaic performance. This enabled us to synthesize a donor–acceptor conjugated copolymer with high solubility, molecular weight, regioregularity, and fullerene content. We investigated the correlations among the active layer hierarchical structure given by the inclusion of electrospun nanofibers and the solar cell photovoltaic properties. The results indicated that SMOC efficiency can be strongly increased by optimizing the supramolecular and nanoscale structure of the active layer, while achieving the highest reported efficiency value (PCE = 5.58%). The enhanced performance may be attributed to well-packed and properly oriented polymer chains. Overall, our work demonstrates that the active material structure optimization obtained by including electrospun nanofibers plays a pivotal role in the development of efficient SMOCs and suggests an interesting perspective for the improvement of copolymer-based photovoltaic device performance using an alternative pathway.

Affiliations:
Pierini F.-IPPT PAN
Lanzi M.-University of Bologna (IT)
Nakielski P.-IPPT PAN
Pawłowska S.-IPPT PAN
Urbanek O.-IPPT PAN
Zembrzycki K.-IPPT PAN
Kowalewski T.A.-IPPT PAN
4.Pawłowska S., Nakielski P., Pierini F., Piechocka I.K., Zembrzycki K., Kowalewski T.A., Lateral migration of electrospun hydrogel nanofilaments in an oscillatory flow, PLOS ONE, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0187815, Vol.12, No.11, pp.1-21, 2017
Abstract:

The recent progress in bioengineering has created great interest in the dynamics and manipulation of long, deformable macromolecules interacting with fluid flow. We report experimental data on the cross-flow migration, bending, and buckling of extremely deformable hydrogel nanofilaments conveyed by an oscillatory flow into a microchannel. The changes in migration velocity and filament orientation are related to the flow velocity and the filament’s initial position, deformation, and length. The observed migration dynamics of hydrogel filaments qualitatively confirms the validity of the previously developed worm-like bead-chain hydrodynamic model. The experimental data collected may help to verify the role of hydrodynamic interactions in molecular simulations of long molecular chains dynamics.

Affiliations:
Pawłowska S.-IPPT PAN
Nakielski P.-IPPT PAN
Pierini F.-IPPT PAN
Piechocka I.K.-IPPT PAN
Zembrzycki K.-IPPT PAN
Kowalewski T.A.-IPPT PAN
5.Pierini F., Lanzi M., Nakielski P., Pawłowska S., Zembrzycki K., Kowalewski T.A., Electrospun poly(3-hexylthiophene)/poly(ethylene oxide)/graphene oxide composite nanofibers: effects of graphene oxide reduction, Polymers for Advanced Technologies, ISSN: 1042-7147, DOI: 10.1002/pat.3816, Vol.27, No.11, pp.1465-1475, 2016
Abstract:

In this article, we report on the production by electrospinning of P3HT/PEO, P3HT/PEO/GO, and P3HT/PEO/rGO nanofibers in which the filler is homogeneously dispersed and parallel oriented along the fibers axis. The effect of nanofillers' presence inside nanofibers and GO reduction was studied, in order to reveal the influence of the new hierarchical structure on the electrical conductivity and mechanical properties. An in-depth characterization of the purity and regioregularity of the starting P3HT as well as the morphology and chemical structure of GO and rGO was carried out. The morphology of the electrospun nanofibers was examined by both scanning and transmission electron microscopy. The fibrous nanocomposites are also characterized by differential scanning calorimetry to investigate their chemical structure and polymer chains arrangements. Finally, the electrical conductivity of the electrospun fibers and the elastic modulus of the single fibers are evaluated using a four-point probe method and atomic force microscopy nanoindentation, respectively. The electrospun materials crystallinity as well as the elastic modulus increase with the addition of the nanofillers while the electrical conductivity is positively influenced by the GO reduction.

Keywords:

electrospun composite nanofibers, poly(3-hexylthiophene), graphene oxide, electrical conductivity, mechanical properties

Affiliations:
Pierini F.-IPPT PAN
Lanzi M.-University of Bologna (IT)
Nakielski P.-IPPT PAN
Pawłowska S.-IPPT PAN
Zembrzycki K.-IPPT PAN
Kowalewski T.A.-IPPT PAN
6.Pierini F., Zembrzycki K., Nakielski P., Pawłowska S., Kowalewski T.A., Atomic force microscopy combined with optical tweezers (AFM/OT), MEASUREMENT SCIENCE AND TECHNOLOGY, ISSN: 0957-0233, DOI: 10.1088/0957-0233/27/2/025904, Vol.27, pp.025904-1-11, 2016
Abstract:

The role of mechanical properties is essential to understand molecular, biological materials, and nanostructures dynamics and interaction processes. Atomic force microscopy (AFM) is the most commonly used method of direct force evaluation, but due to its technical limitations this single probe technique is unable to detect forces with femtonewton resolution. In this paper we present the development of a combined atomic force microscopy and optical tweezers (AFM/OT) instrument. The focused laser beam, on which optical tweezers are based, provides us with the ability to manipulate small dielectric objects and to use it as a high spatial and temporal resolution displacement and force sensor in the same AFM scanning zone. We demonstrate the possibility to develop a combined instrument with high potential in nanomechanics, molecules manipulation and biological studies. AFM/OT equipment is described and characterized by studying the ability to trap dielectric objects and quantifying the detectable and applicable forces. Finally, optical tweezers calibration methods and instrument applications are given.

Keywords:

optical trap, nanomanipulation, nanomechanics, femtonewton forces

Affiliations:
Pierini F.-IPPT PAN
Zembrzycki K.-IPPT PAN
Nakielski P.-IPPT PAN
Pawłowska S.-IPPT PAN
Kowalewski T.A.-IPPT PAN
7.Nakielski P., Pawłowska S., Pierini F., Liwińska W., Hejduk P., Zembrzycki K., Zabost E., Kowalewski T.A., Hydrogel nanofilaments via core-shell electrospinning, PLOS ONE, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0129816, Vol.10, No.6, pp.e0129816-1-16, 2015
Abstract:

Recent biomedical hydrogels applications require the development of nanostructures with controlled diameter and adjustable mechanical properties. Here we present a technique for the production of flexible nanofilaments to be used as drug carriers or in microfluidics, with deformability and elasticity resembling those of long DNA chains. The fabrication method is based on the core-shell electrospinning technique with core solution polymerisation post electrospinning. Produced from the nanofibers highly deformable hydrogel nanofilaments are characterised by their Brownian motion and bending dynamics. The evaluated mechanical properties are compared with AFM nanoindentation tests.

Correction: Hydrogel Nanofilaments via Core-Shell Electrospinning, Nakielski P., Pawłowska S., Pierini F., Liwińska W., Hejduk P., Zembrzycki K., Zabost E., Kowalewski T.A., PLOS ONE, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0133458, Vol.10, No.7, pp.e0133458-1-2, 2015

Keywords:

Gels, Nanomaterials, Atomic force microscopy, Polymerization, Bending, Mass diffusivity, Mechanical properties, Hydrodynamics

Affiliations:
Nakielski P.-IPPT PAN
Pawłowska S.-IPPT PAN
Pierini F.-IPPT PAN
Liwińska W.-other affiliation
Hejduk P.-other affiliation
Zembrzycki K.-IPPT PAN
Zabost E.-University of Warsaw (PL)
Kowalewski T.A.-IPPT PAN
8.Samborski A., Jankowski P., Węgrzyn J., Michalski J.A., Pawłowska S., Jakieła S., Garstecki P., Blood diagnostics using sedimentation to extract plasma on a fully integrated point-of-care microfluidic system, Engineering in Life Sciences, ISSN: 1618-0240, DOI: 10.1002/elsc.201400077, Vol.15, No.3, pp.333-339, 2015
Abstract:

Blood is the richest source of diagnostic information. The growing interest in point-of-care analytics prompted several attempts to extract plasma from whole blood in simple diagnostic devices. The simplest method of separation is sedimentation. Here we show the first microfluidic system that uses sedimentation to extract plasma from undiluted blood and integrates execution of liquid assays on the extracted material. We present a microfluidic chip that accepts a small sample (27 μL) of whole blood, separates up to 6 μL of plasma, and uses metered volumes of plasma and of reagent (2-chloro-4-nitrophenyl-α-maltotrioside, CNP-G3) for a liquid enzymatic assay. With a custom designed channel, the system separates blood by sedimentation within few minutes of accepting the sample, mixes it with the reagent, and quantifies spectrophotometrically the product of the enzymatic reaction. As a model demonstration, we show a quantitative enzymatic α-amylase assay that is routinely used in diagnosis of pancreas diseases. The paper reports the design and characterization of the microfluidic device and the results of tests on clinically collected blood samples. The results obtained with the microfluidic system compare well to a reference bench-top analyzer.

Affiliations:
Samborski A.-other affiliation
Jankowski P.-Institute of Physical Chemistry, Polish Academy of Sciences (PL)
Węgrzyn J.-other affiliation
Michalski J.A.-Warsaw University of Technology (PL)
Pawłowska S.-IPPT PAN
Jakieła S.-Institute of Physical Chemistry, Polish Academy of Sciences (PL)
Garstecki P.-Institute of Physical Chemistry, Polish Academy of Sciences (PL)

List of recent monographs
1.
578
Pawłowska S., DIFFUSION AND MIGRATION OF NANO PARTICLES AND FILAMENTS SUSPENDED IN OSCILLATING FLOW, IPPT Reports on Fundamental Technological Research, 2, pp.1-106, 2018
2.
471
Zembrzycki K., Pawłowska S., Nakielski P., Pierini F., Development of a hybrid Atomic Force microscope and Optical Tweezers apparatus, IPPT Reports on Fundamental Technological Research, 2, pp.1-58, 2016

Conference papers
1.Kowalewski T.A., Nakielski P., Pierini F., Zembrzycki K., Pawłowska S., Micro and nano fluid mechanics, CMM, 3rd Polish Congress of Mechanics and 21st International Conference on Computer Methods in Mechanics , 2015-09-08/09-11, Gdańsk (PL), pp.27-34, 2016
Abstract:

The current task of contemporary fluid mechanics evidently moves from modeling large scale turbulence to lower, molecular scale limit, where assumption of a continuous and deterministic description becomes questionable again. Once the scaling length of flow becomes comparable with structure dimensions, transport phenomena are strongly modulated by molecular interactions and its proper interpretation needs involvement of deeper physics. New experimental tools largely help in understanding transport phenomena at nanoscales. In the following review we give few examples of problems appealing for new theoretical and numerical models embracing continuous flow modeling with molecular scale phenomena.

Affiliations:
Kowalewski T.A.-IPPT PAN
Nakielski P.-IPPT PAN
Pierini F.-IPPT PAN
Zembrzycki K.-IPPT PAN
Pawłowska S.-IPPT PAN
2.Pawłowska S., Highly deformable nanofilaments in flow, JOURNAL OF PHYSICS: CONFERENCE SERIES, ISSN: 1742-6588, DOI: 10.1088/1742-6596/760/1/012022, Vol.760, pp.1-10, 2016
Abstract:

Experimental analysis of hydrogel nanofilaments conveyed by flow is conducted to help in understanding physical phenomena responsible for transport properties and shape deformations of long bio-objects, like DNA or proteins. Investigated hydrogel nanofilaments exhibit typical macromolecules-like behavior, as spontaneous conformational changes and cross-flow migration. Results of the experiments indicate critical role of thermal fluctuations behavior of single filaments.

Affiliations:
Pawłowska S.-IPPT PAN

Conference abstracts
1.Pawłowska S., Pierini F., Kowalewski T.A., Highly flexible filaments in an oscillatory microchannel flow, EFMC12, 12th European Fluid Mechanics Conference, 2018-09-09/09-13, Wiedeń (AT), pp.1, 2018
2.Pierini F., Lanzi M., Nakielski P., Pawłowska S., Urbanek O., Kowalewski T.A., Electrospinning of polythiophene with pendant fullerene nanofibers for single-material organic solar cells, Electrospin2018 International Conference, 2018-01-16/01-18, Stellenbosch (ZA), pp.19-20, 2018
3.Pawłowska S., Nakielski P., Pierini F., Electrospun polyacrylamide hydrogel nanofibers: from nanocarriers to stimuli responsive nanomaterials, Electrospin2018 International Conference, 2018-01-16/01-18, Stellenbosch (ZA), pp.39, 2018
4.Pawłowska S., Pierini F., Nakielski P., Piechocka I., Zembrzycki K., Kowalewski T.A., Hydrogel nanofilaments in oscillatory microchannel flow, ISFV 18, 18th International Symposium on Flow Visualization, 2018-06-26/06-29, Zurich (CH), pp.233-235, 2018
5.Pierini F., Nakielski P., Pawłowska S., Piechocka I., Zembrzycki K., Kowalewski T.A., Development and applications of atomic force microscopy combined with optical tweezers (AFM/OT), AFM BioMed, 8th AFM BioMed Conference, 2017-09-04/09-08, Kraków (PL), pp.103, 2017
Abstract:

Atomic force microscopy (AFM) is an evolution of scanning tunnelling microscopy that immediately gained popularity thanks to its ability to analyse nanomaterials. Initially, AFM was developed for nanomaterials imaging purposes, however the development of new features made it the most commonly used tool for studying the biophysical properties of biological samples. On the other hand, atomic force microscopy has limited use for examining sub-piconewton forces. Few techniques have been developed to measure forces below the AFM limit of detection. Among them, optical tweezers (OT) stand out for their high resolution, flexibility, and because they make it possible to accurately manipulate biological samples and carry out biophysics experiments without side effects thanks to their non-invasive properties.
The combination of AFM with other techniques in the last decades has significantly extended its capability. The improvement of the AFM force resolution by developing a hybrid double probe instrument based on the combination of AFM and OT has great potential in cell or molecular biology. [1]
We outline principles of atomic force microscopy combined with optical tweezers (AFM/OT) developed by our team underlying the techniques applied during the design, building and instrument use stages. We describe the experimental procedure for calibration of the system and we prove the achievement of a higher resolution (force: 10 fN – spatial: 0.1 nm – temporal: 10 ns) than the stand alone AFM.
We show the use of the hybrid equipment in a number of different biophysics experiments performed employing both AFM and OT probes. The presented studies include the demonstration of simultaneous high-precision nanomanipulation and imaging, the evaluation of single biomolecule mechanical properties and the single cell membrane activation and probing. Finally, we show the further potential applications of our AFM/OT.

Keywords:

AFM, Optical Tweezers

Affiliations:
Pierini F.-IPPT PAN
Nakielski P.-IPPT PAN
Pawłowska S.-IPPT PAN
Piechocka I.-IPPT PAN
Zembrzycki K.-IPPT PAN
Kowalewski T.A.-IPPT PAN
6.Pawłowska S., Nakielski P., Pierini F., Zembrzycki K., Piechocka I.K., Kowalewski T.A., Tumbling, rotating and coiling of nanofilaments in an oscillating microchannel flow, BioNano6, Biomolecules and Nanostructures 6, 2017-05-10/05-14, Podlesice (PL), No.41E, pp.60, 2017
7.Pawłowska S., Pierini F., Nakielski P., Piechocka I., Zembrzycki K., Kowalewski T.A., Lateral Migration of Highly Deformable Nanofilaments Conveyed by Oscillatory Flow, CNM, 5th Conference on Nano- and Micromechanics, 2017-07-04/07-06, Wrocław (PL), pp.29-31, 2017
Keywords:

thermal fluctuations, lateral migration, flexible filaments

Affiliations:
Pawłowska S.-IPPT PAN
Pierini F.-IPPT PAN
Nakielski P.-IPPT PAN
Piechocka I.-IPPT PAN
Zembrzycki K.-IPPT PAN
Kowalewski T.A.-IPPT PAN
8.Pawłowska S., Kowalewski T.A., Lateral migration of solid spheroidal nanoparticles and highly deformable hydrogel nanofilaments under the influence of oscillatory flow, ExFM2017, Experiments in Fluid Mechanics, 2017-10-23/10-24, Warszawa (PL), pp.1-2, 2017
9.Pawłowska S., Nakielski P., Pierini F., Zembrzycki K., Kowalewski T.A., Mobility of highly deformable nanofilaments, ICTAM XXIV, 24th International Congress of Theoretical and Applied Mechanics, 2016-08-21/08-26, Montréal (CA), pp.1196-1197, 2016
Abstract:

Mobility of hydrogel nanofilaments suspended in liquid is investigated to gain basic knowledge on hydrodynamic interactions biased by Brownian fluctuations. Typical for long macromolecules effects like spontaneous conformational changes and cross-flow migration are observed and evaluated. The collected experimental data can be used to validate assumptions present in numerical models describing intercellular transport of long biomolecules.

Keywords:

persistence length, macromolecules, electrospinning, DNA, Brownian motion

Affiliations:
Pawłowska S.-IPPT PAN
Nakielski P.-IPPT PAN
Pierini F.-IPPT PAN
Zembrzycki K.-IPPT PAN
Kowalewski T.A.-IPPT PAN
10.Pierini F., Nakielski P., Pawłowska S., Zembrzycki K., Kowalewski T.A., Particles double layer evaluation by atomic force microscopy - optical tweezers, ICTAM XXIV, 24th International Congress of Theoretical and Applied Mechanics, 2016-08-21/08-26, Montréal (CA), pp.1204-1205, 2016
Abstract:

Atomic force microscopy (AFM) is the most commonly used method of direct force evaluation, but due to its technical limitations this single probe technique is unable to detect forces with femtonewton resolution. We present the development of a combined atomic force microscopy and optical tweezers (AFM/OT) instrument. The optical tweezers system provides us the ability to manipulate small dielectric objects and to use it as a high spatial and temporal resolution displacement and force sensor in the same AFM scanning zone. We demonstrate the possibility to develop a combined instrument with high potential in nanomechanics, molecules manipulation and biologic al studies. The presented study is aimed to quantify the interaction forces between two single polystyrene particles in the femtonewton scale by using the developed AFM/OT equipment.

Keywords:

optical trap, nanomanipulation, femtonewtons

Affiliations:
Pierini F.-IPPT PAN
Nakielski P.-IPPT PAN
Pawłowska S.-IPPT PAN
Zembrzycki K.-IPPT PAN
Kowalewski T.A.-IPPT PAN
11.Pawłowska S., Nakielski P., Pierini F., Zembrzycki K., Kowalewski T.A., Highly Deformable Hydrogel Nanofilaments in Poiseuille Flow, MNF 2016, 5th Micro and Nano Flows Conference, 2016-09-11/09-14, Milan (IT), pp.50, 2016
Keywords:

Hydrogel Nanofilaments, Bending Dynamics, Poiseuille Flow, Electrospinning

Affiliations:
Pawłowska S.-IPPT PAN
Nakielski P.-IPPT PAN
Pierini F.-IPPT PAN
Zembrzycki K.-IPPT PAN
Kowalewski T.A.-IPPT PAN
12.Pierini F., Zembrzycki K., Nakielski P., Pawłowska S., Kowalewski T.A., Nanomanipulating and sensing single particles interactions with combined atomic force microscopy optical tweezers (AFM/OT), MNF 2016, 5th Micro and Nano Flows Conference, 2016-09-11/09-14, Milan (IT), pp.40-41, 2016
Keywords:

Atomic force microscopy/optical tweezers, Nanomanipulation, Single particles analysis, Interaction force measurement, DLVO theory

Affiliations:
Pierini F.-IPPT PAN
Zembrzycki K.-IPPT PAN
Nakielski P.-IPPT PAN
Pawłowska S.-IPPT PAN
Kowalewski T.A.-IPPT PAN
13.Pawłowska S., Highly deformable nanofilaments in flow, XXII Fluid Mechanics Conference, 2016-09-11/09-14, Słok k/Bełchatowa (PL), pp.79-80, 2016
14.Nakielski P., Pawłowska S., Pierini F., Hejduk P., Zembrzycki K., Kowalewski T.A., Novel hydrogel nanofilaments based on electrospun core-shell fibers, Europhysics Conference Biomolecules and Nanostructures 5, 2015-05-13/05-17, Jaroszowice (PL), Vol.39C, pp.101, 2015
Keywords:

nanofilaments, hydrogel, long molecules flexibility

Affiliations:
Nakielski P.-IPPT PAN
Pawłowska S.-IPPT PAN
Pierini F.-IPPT PAN
Hejduk P.-other affiliation
Zembrzycki K.-IPPT PAN
Kowalewski T.A.-IPPT PAN
15.Pawłowska S., Nakielski P., Pierini F., Zembrzycki K., Kowalewski T.A., Mobility of nanofilaments, Experiments in Fluid Mechanics 2015, 2015-10-26/10-27, Warszawa (PL), pp.1, 2015
Abstract:

We propose a microscale experimental model in form of highly deformable nanofilaments, which permits for precise optical measurements and to evaluate hydrodynamic interactions (mobility). The conducted research includes determination of the mechanical properties of elastic hydrogel nanofilaments obtained by electrospinning that can serve as experimental benchmark to validate theoretical and numerical models describing dynamics of long biological molecules (e.g. proteins, DNA). Nanofilaments mechanical properties are determined by studying their dynamic bending. in shear flow and deformations due to the thermal fluctuations (Brownian motion). These results are compared with AFM nanoindentation measurements. Data obtained from this research project will be a base to crea te biocompatible nanoobjects that can become tools for the regeneration of tissue (e.g. neural tissue).

Keywords:

Biocompatible nanoobjects, highly deformable nanofilaments, regeneration of tissue

Affiliations:
Pawłowska S.-IPPT PAN
Nakielski P.-IPPT PAN
Pierini F.-IPPT PAN
Zembrzycki K.-IPPT PAN
Kowalewski T.A.-IPPT PAN
16.Nakielski P., Pawłowska S., Pierini F., Hejduk P., Zembrzycki K., Kowalewski T.A., Processing and mechanical properties relationships in hydrogel nanofilamets for biological application, ICMCSF, International Conference on Mechanics of Complex Solids and Fluids, 2015-05-17/05-22, Lille (FR), pp.1, 2015
Keywords:

Nanofilaments, hydrogel filaments, nanofibres, long nanoobjects deformability

Affiliations:
Nakielski P.-IPPT PAN
Pawłowska S.-IPPT PAN
Pierini F.-IPPT PAN
Hejduk P.-other affiliation
Zembrzycki K.-IPPT PAN
Kowalewski T.A.-IPPT PAN
17.Pierini F., Nakielski P., Pawłowska S., Zembrzycki K., Kowalewski T.A., Hydrogel nanofilaments via core-shell electrospinning, NanoItaly, Nanotechnology meeting forum for research and market, 2015-09-21/09-24, Roma (IT), pp.1, 2015
Abstract:

Controlled drug delivery systems are used to improve the conventional administration of drugs. One of the main challenges is to synthesize materials able to find a defined target and to release drugs in a controlled manner [1]. Several research tasks have been focused on developing ideal drug delivery systems made by hydrogel due to their unique properties [2]. The present study is based on the idea that soft and flexible nanomaterials can easily travel in crowed environments of body fluids and biological tissues. Modification of their mechanical properties obtained by changing of the cross-linker amount may give us the possibility to tune the material rigidity according to desired application. Here, we describe a novel method based on coaxial electrospinning for obtaining highly flexible hydrogel nanofilaments able to transport and release dedicated molecules. Two different types of hydrogels (poly(N,Nisopropyl acrylamide) and polyacrylamide) with three polymer/cross-linker ratios were produced and deeply studied. The nanofilaments morphology was characterized and the release of bovine serum albumin as a function of time was quantified. Mechanical properties of highly deformable hydrogel nanofilaments were evaluated by bending dynamics and Brownian motion observation techniques. The calculated mechanical properties were compared with data obtained by nanoindention. The results highlight the crucial role of morphology and stiffness on mobility of nanofilaments colloid systems. The information gained are fundamental to design nanoobjects with well-defined chemical and physical behaviour.

Keywords:

Nanofilaments, electrospinning, core-shell method, hydrogel

Affiliations:
Pierini F.-IPPT PAN
Nakielski P.-IPPT PAN
Pawłowska S.-IPPT PAN
Zembrzycki K.-IPPT PAN
Kowalewski T.A.-IPPT PAN
18.Kowalewski T.A., Nakielski P., Pierini F., Zembrzycki K., Pawłowska S., Nanoscale challenges of fluid mechanics, PCM-CMM-2015, 3rd Polish Congress of Mechanics and 21st Computer Methods in Mechanics, 2015-09-08/09-11, Gdańsk (PL), pp.11-16, 2015
Abstract:

In this talk we would like to tackle general question of contemporary fluid dynamics, how far its assumption of a continuous, smooth medium remains useful when size and time scales start to approach molecular ones. The question is not trivial and seems to depend on several additional factors usually minored. For example, when full Navier-Stokes equations are replaced by their linear approximation we are loosing basic characteristics of convective motion, and still we use such approach. Once our fluid becomes granular matter with its own internal properties, proper interpretation of flow interactions with other molecular structures probably needs deeper physics. But still we try to convert such problem to the classical macro/micro scale description. Hence a general question arises, how small does a fluid have to be before it is not a fluid anymore?

Keywords:

microfluidics, nanofluids, Brownian motion, nanofilaments

Affiliations:
Kowalewski T.A.-IPPT PAN
Nakielski P.-IPPT PAN
Pierini F.-IPPT PAN
Zembrzycki K.-IPPT PAN
Pawłowska S.-IPPT PAN
19.Pawłowska S., Nakielski P., Hejduk P., Pierini F., Zembrzycki K., Kowalewski T.A., Brownian motion of nanofibers, KKNM, 4th National Conference on Nano- and Micromechanics, 2014-07-08/07-10, Wrocław (PL), pp.162-163, 2014
Keywords:

electrospinning, flexible nanorods, Brownian motion

Affiliations:
Pawłowska S.-IPPT PAN
Nakielski P.-IPPT PAN
Hejduk P.-other affiliation
Pierini F.-IPPT PAN
Zembrzycki K.-IPPT PAN
Kowalewski T.A.-IPPT PAN
20.Pierini F., Hejduk P., Nakielski P., Pawłowska S., Zembrzycki K., Kowalewski T.A., Study of surface interaction forces in polystyrene colloidal nanoparticles systems, XXI FMC, XXI Fluid Mechanics Conference, 2014-06-15/06-18, Kraków (PL), pp.115, 2014
Keywords:

Nanoparticles, polystyrene beads, surface properties, atomic force microscopy, hydrodynamic properties

Affiliations:
Pierini F.-IPPT PAN
Hejduk P.-other affiliation
Nakielski P.-IPPT PAN
Pawłowska S.-IPPT PAN
Zembrzycki K.-IPPT PAN
Kowalewski T.A.-IPPT PAN
21.Pawłowska S., Hejduk P., Nakielski P., Pierini F., Zembrzycki K., Kowalewski T.A., Analysis of nanoparticles hydrodynamic diameters in Brownian motion, XXI FMC, XXI Fluid Mechanics Conference, 2014-06-15/06-18, Kraków (PL), pp.116, 2014
Keywords:

Nanoparticles, Brownian motion, hydrodynamic diameter

Affiliations:
Pawłowska S.-IPPT PAN
Hejduk P.-other affiliation
Nakielski P.-IPPT PAN
Pierini F.-IPPT PAN
Zembrzycki K.-IPPT PAN
Kowalewski T.A.-IPPT PAN