Sylwia Pawłowska, Ph.D., Eng.

Department of Biosystems and Soft Matter (ZBiMM)
Division of Modelling in Biology and Medicine (PMBM)
position: assistant professor
telephone: (+48) 22 826 12 81 ext.: 330
room: 320
e-mail: spaw

Doctoral thesis
2018-12-11Dynamics of nano objects suspended in liquids: experimental analysis  (IBIB PAN)
supervisor -- Prof. Tomasz Kowalewski, Ph.D., Dr. Habil., IPPT PAN
supervisor -- Prof. Dr. Filippo Pierini, IPPT PAN
1262
 
Recent publications
1.Pierini F., Nakielski P., Urbanek O., Pawłowska S., Lanzi M., De Sio L., Kowalewski T.A., Polymer-Based Nanomaterials for Photothermal Therapy: From Light-Responsive to Multifunctional Nanoplatforms for Synergistically Combined Technologies, BIOMACROMOLECULES, ISSN: 1525-7797, DOI: 10.1021/acs.biomac.8b01138, Vol.19, No.11, pp.4147-4167, 2018
Abstract:

Materials for the treatment of cancer have been studied comprehensively over the past few decades. Among the various kinds of biomaterials, polymer-based nanomaterials represent one of the most interesting research directions in nanomedicine because their controlled synthesis and tailored designs make it possible to obtain nanostructures with biomimetic features and outstanding biocompatibility. Understanding the chemical and physical mechanisms behind the cascading stimuli-responsiveness of smart polymers is fundamental for the design of multifunctional nanomaterials to be used as photothermal agents for targeted polytherapy. In this review, we offer an in-depth overview of the recent advances in polymer nanomaterials for photothermal therapy, describing the features of three different types of polymer-based nanomaterials. In each case, we systematically show the relevant benefits, highlighting the strategies for developing light-controlled multifunctional nanoplatforms that are responsive in a cascade manner and addressing the open issues by means of an inclusive state-of-the-art review. Moreover, we face further challenges and provide new perspectives for future strategies for developing novel polymeric nanomaterials for photothermally assisted therapies.

Affiliations:
Pierini F.-IPPT PAN
Nakielski P.-IPPT PAN
Urbanek O.-IPPT PAN
Pawłowska S.-IPPT PAN
Lanzi M.-University of Bologna (IT)
De Sio L.-Sapienza University of Rome (IT)
Kowalewski T.A.-IPPT PAN
2.Pawłowska S., Kowalewski T.A., Pierini F., Fibrous polymer nanomaterials for biomedical applications and their transport by fluids: an overview, SOFT MATTER, ISSN: 1744-683X, DOI: 10.1039/C8SM01269E, Vol.14, pp.8421-8444, 2018
Abstract:

Over the past few decades, there has been strong interest in the development of new micro- and nanomaterials for biomedical applications. Their use in the form of capsules, particles or filaments suspended in body fluids is associated with conformational changes and hydrodynamic interactions responsible for their transport. The dynamics of fibres or other long objects in Poiseuille flow is one of the fundamental problems in a variety of biomedical contexts, such as mobility of proteins, dynamics of DNA or other biological polymers, cell movement, tissue engineering, and drug delivery. In this review, we discuss several important applications of micro and nanoobjects in this field and try to understand the problems of their transport in flow resulting from material-environment interactions in typical, crowded, and complex biological fluids. Our aim is to elucidate the relationship between the nano- and microscopic structures of elongated polymer particles and their flow properties, thus opening the possibility to design nanoobjects that can be efficiently transported by body fluids for targeted drug release or local tissue regeneration.

Affiliations:
Pawłowska S.-IPPT PAN
Kowalewski T.A.-IPPT PAN
Pierini F.-IPPT PAN
3.Pierini F., Lanzi M., Nakielski P., Pawłowska S., Urbanek O., Zembrzycki K., Kowalewski T.A., Single-Material Organic Solar Cells Based on Electrospun Fullerene-Grafted Polythiophene Nanofibers, Macromolecules, ISSN: 0024-9297, DOI: 10.1021/acs.macromol.7b00857, Vol.50, No.13, pp.4972-4981, 2017
Abstract:

Highly efficient single-material organic solar cells (SMOCs) based on fullerene-grafted polythiophenes were fabricated by incorporating electrospun one-dimensional (1D) nanostructures obtained from polymer chain stretching. Poly(3-alkylthiophene) chains were chemically tailored in order to reduce the side effects of charge recombination which severely affected SMOC photovoltaic performance. This enabled us to synthesize a donor–acceptor conjugated copolymer with high solubility, molecular weight, regioregularity, and fullerene content. We investigated the correlations among the active layer hierarchical structure given by the inclusion of electrospun nanofibers and the solar cell photovoltaic properties. The results indicated that SMOC efficiency can be strongly increased by optimizing the supramolecular and nanoscale structure of the active layer, while achieving the highest reported efficiency value (PCE = 5.58%). The enhanced performance may be attributed to well-packed and properly oriented polymer chains. Overall, our work demonstrates that the active material structure optimization obtained by including electrospun nanofibers plays a pivotal role in the development of efficient SMOCs and suggests an interesting perspective for the improvement of copolymer-based photovoltaic device performance using an alternative pathway.

Affiliations:
Pierini F.-IPPT PAN
Lanzi M.-University of Bologna (IT)
Nakielski P.-IPPT PAN
Pawłowska S.-IPPT PAN
Urbanek O.-IPPT PAN
Zembrzycki K.-IPPT PAN
Kowalewski T.A.-IPPT PAN
4.Pawłowska S., Nakielski P., Pierini F., Piechocka I.K., Zembrzycki K., Kowalewski T.A., Lateral migration of electrospun hydrogel nanofilaments in an oscillatory flow, PLOS ONE, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0187815, Vol.12, No.11, pp.1-21, 2017
Abstract:

The recent progress in bioengineering has created great interest in the dynamics and manipulation of long, deformable macromolecules interacting with fluid flow. We report experimental data on the cross-flow migration, bending, and buckling of extremely deformable hydrogel nanofilaments conveyed by an oscillatory flow into a microchannel. The changes in migration velocity and filament orientation are related to the flow velocity and the filament’s initial position, deformation, and length. The observed migration dynamics of hydrogel filaments qualitatively confirms the validity of the previously developed worm-like bead-chain hydrodynamic model. The experimental data collected may help to verify the role of hydrodynamic interactions in molecular simulations of long molecular chains dynamics.

Affiliations:
Pawłowska S.-IPPT PAN
Nakielski P.-IPPT PAN
Pierini F.-IPPT PAN
Piechocka I.K.-IPPT PAN
Zembrzycki K.-IPPT PAN
Kowalewski T.A.-IPPT PAN
5.Pierini F., Lanzi M., Nakielski P., Pawłowska S., Zembrzycki K., Kowalewski T.A., Electrospun poly(3-hexylthiophene)/poly(ethylene oxide)/graphene oxide composite nanofibers: effects of graphene oxide reduction, Polymers for Advanced Technologies, ISSN: 1042-7147, DOI: 10.1002/pat.3816, Vol.27, No.11, pp.1465-1475, 2016
Abstract:

In this article, we report on the production by electrospinning of P3HT/PEO, P3HT/PEO/GO, and P3HT/PEO/rGO nanofibers in which the filler is homogeneously dispersed and parallel oriented along the fibers axis. The effect of nanofillers' presence inside nanofibers and GO reduction was studied, in order to reveal the influence of the new hierarchical structure on the electrical conductivity and mechanical properties. An in-depth characterization of the purity and regioregularity of the starting P3HT as well as the morphology and chemical structure of GO and rGO was carried out. The morphology of the electrospun nanofibers was examined by both scanning and transmission electron microscopy. The fibrous nanocomposites are also characterized by differential scanning calorimetry to investigate their chemical structure and polymer chains arrangements. Finally, the electrical conductivity of the electrospun fibers and the elastic modulus of the single fibers are evaluated using a four-point probe method and atomic force microscopy nanoindentation, respectively. The electrospun materials crystallinity as well as the elastic modulus increase with the addition of the nanofillers while the electrical conductivity is positively influenced by the GO reduction.

Keywords:

electrospun composite nanofibers, poly(3-hexylthiophene), graphene oxide, electrical conductivity, mechanical properties

Affiliations:
Pierini F.-IPPT PAN
Lanzi M.-University of Bologna (IT)
Nakielski P.-IPPT PAN
Pawłowska S.-IPPT PAN
Zembrzycki K.-IPPT PAN
Kowalewski T.A.-IPPT PAN
6.Pierini F., Zembrzycki K., Nakielski P., Pawłowska S., Kowalewski T.A., Atomic force microscopy combined with optical tweezers (AFM/OT), MEASUREMENT SCIENCE AND TECHNOLOGY, ISSN: 0957-0233, DOI: 10.1088/0957-0233/27/2/025904, Vol.27, pp.025904-1-11, 2016
Abstract:

The role of mechanical properties is essential to understand molecular, biological materials, and nanostructures dynamics and interaction processes. Atomic force microscopy (AFM) is the most commonly used method of direct force evaluation, but due to its technical limitations this single probe technique is unable to detect forces with femtonewton resolution. In this paper we present the development of a combined atomic force microscopy and optical tweezers (AFM/OT) instrument. The focused laser beam, on which optical tweezers are based, provides us with the ability to manipulate small dielectric objects and to use it as a high spatial and temporal resolution displacement and force sensor in the same AFM scanning zone. We demonstrate the possibility to develop a combined instrument with high potential in nanomechanics, molecules manipulation and biological studies. AFM/OT equipment is described and characterized by studying the ability to trap dielectric objects and quantifying the detectable and applicable forces. Finally, optical tweezers calibration methods and instrument applications are given.

Keywords:

optical trap, nanomanipulation, nanomechanics, femtonewton forces

Affiliations:
Pierini F.-IPPT PAN
Zembrzycki K.-IPPT PAN
Nakielski P.-IPPT PAN
Pawłowska S.-IPPT PAN
Kowalewski T.A.-IPPT PAN
7.Nakielski P., Pawłowska S., Pierini F., Liwińska W., Hejduk P., Zembrzycki K., Zabost E., Kowalewski T.A., Hydrogel nanofilaments via core-shell electrospinning, PLOS ONE, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0129816, Vol.10, No.6, pp.e0129816-1-16, 2015
Abstract:

Recent biomedical hydrogels applications require the development of nanostructures with controlled diameter and adjustable mechanical properties. Here we present a technique for the production of flexible nanofilaments to be used as drug carriers or in microfluidics, with deformability and elasticity resembling those of long DNA chains. The fabrication method is based on the core-shell electrospinning technique with core solution polymerisation post electrospinning. Produced from the nanofibers highly deformable hydrogel nanofilaments are characterised by their Brownian motion and bending dynamics. The evaluated mechanical properties are compared with AFM nanoindentation tests.

Correction: Hydrogel Nanofilaments via Core-Shell Electrospinning, Nakielski P., Pawłowska S., Pierini F., Liwińska W., Hejduk P., Zembrzycki K., Zabost E., Kowalewski T.A., PLOS ONE, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0133458, Vol.10, No.7, pp.e0133458-1-2, 2015

Keywords:

Gels, Nanomaterials, Atomic force microscopy, Polymerization, Bending, Mass diffusivity, Mechanical properties, Hydrodynamics

Affiliations:
Nakielski P.-IPPT PAN
Pawłowska S.-IPPT PAN
Pierini F.-IPPT PAN
Liwińska W.-other affiliation
Hejduk P.-other affiliation
Zembrzycki K.-IPPT PAN
Zabost E.-University of Warsaw (PL)
Kowalewski T.A.-IPPT PAN
8.Samborski A., Jankowski P., Węgrzyn J., Michalski J.A., Pawłowska S., Jakieła S., Garstecki P., Blood diagnostics using sedimentation to extract plasma on a fully integrated point-of-care microfluidic system, Engineering in Life Sciences, ISSN: 1618-0240, DOI: 10.1002/elsc.201400077, Vol.15, No.3, pp.333-339, 2015
Abstract:

Blood is the richest source of diagnostic information. The growing interest in point-of-care analytics prompted several attempts to extract plasma from whole blood in simple diagnostic devices. The simplest method of separation is sedimentation. Here we show the first microfluidic system that uses sedimentation to extract plasma from undiluted blood and integrates execution of liquid assays on the extracted material. We present a microfluidic chip that accepts a small sample (27 μL) of whole blood, separates up to 6 μL of plasma, and uses metered volumes of plasma and of reagent (2-chloro-4-nitrophenyl-α-maltotrioside, CNP-G3) for a liquid enzymatic assay. With a custom designed channel, the system separates blood by sedimentation within few minutes of accepting the sample, mixes it with the reagent, and quantifies spectrophotometrically the product of the enzymatic reaction. As a model demonstration, we show a quantitative enzymatic α-amylase assay that is routinely used in diagnosis of pancreas diseases. The paper reports the design and characterization of the microfluidic device and the results of tests on clinically collected blood samples. The results obtained with the microfluidic system compare well to a reference bench-top analyzer.

Affiliations:
Samborski A.-other affiliation
Jankowski P.-Institute of Physical Chemistry, Polish Academy of Sciences (PL)
Węgrzyn J.-other affiliation
Michalski J.A.-Warsaw University of Technology (PL)
Pawłowska S.-IPPT PAN
Jakieła S.-Institute of Physical Chemistry, Polish Academy of Sciences (PL)
Garstecki P.-Institute of Physical Chemistry, Polish Academy of Sciences (PL)

List of recent monographs
1.
578
Pawłowska S., DIFFUSION AND MIGRATION OF NANO PARTICLES AND FILAMENTS SUSPENDED IN OSCILLATING FLOW, IPPT Reports on Fundamental Technological Research, 2, pp.1-106, 2018
2.
471
Zembrzycki K., Pawłowska S., Nakielski P., Pierini F., Development of a hybrid Atomic Force microscope and Optical Tweezers apparatus, IPPT Reports on Fundamental Technological Research, 2, pp.1-58, 2016

Conference papers
1.Zembrzycki K., Kowalewski T.A., Pawłowska S., Chrzanowska-Giżyńska J., Nowak M., Walczak M., Pierini F., Atomic force microscopy combined with optical tweezers (AFM/OT): characterization of micro and nanomaterial interactions, SPIE Optics + Photonics, 2018-08-21/08-23, San Diego (US), No.10723, pp.1072323-1-6, 2018
Abstract:

Materials containing suspended micro- or nanomaterials are used extensively in multiple fields of research and industry. In order to understand the behavior of nanomaterials suspended in a liquid, the knowledge of particle stability and mobility is fundamental. For this reason, it is necessary to know the nanoscale solid-solid interaction and the hydrodynamic properties of the particles. In the presented research we used a hybrid Atomic Force Microscope coupled with Optical Tweezers system to measure the femtonewton scale interaction forces acting between single particles and the walls of a microchannel at different separation distances and environmental conditions. We show an important improvement in a typical detection system that increases the signal to noise ratio for more accurate position detection at very low separation distances.

Keywords:

Optical Tweezers, Atomic Force Microscopy, particle-wall interaction, colloid stability

Affiliations:
Zembrzycki K.-IPPT PAN
Kowalewski T.A.-IPPT PAN
Pawłowska S.-IPPT PAN
Chrzanowska-Giżyńska J.-IPPT PAN
Nowak M.-IPPT PAN
Walczak M.-IPPT PAN
Pierini F.-IPPT PAN
2.Kowalewski T.A., Nakielski P., Pierini F., Zembrzycki K., Pawłowska S., Micro and nano fluid mechanics, CMM, 3rd Polish Congress of Mechanics and 21st International Conference on Computer Methods in Mechanics , 2015-09-08/09-11, Gdańsk (PL), pp.27-34, 2016
Abstract:

The current task of contemporary fluid mechanics evidently moves from modeling large scale turbulence to lower, molecular scale limit, where assumption of a continuous and deterministic description becomes questionable again. Once the scaling length of flow becomes comparable with structure dimensions, transport phenomena are strongly modulated by molecular interactions and its proper interpretation needs involvement of deeper physics. New experimental tools largely help in understanding transport phenomena at nanoscales. In the following review we give few examples of problems appealing for new theoretical and numerical models embracing continuous flow modeling with molecular scale phenomena.

Affiliations:
Kowalewski T.A.-IPPT PAN
Nakielski P.-IPPT PAN
Pierini F.-IPPT PAN
Zembrzycki K.-IPPT PAN
Pawłowska S.-IPPT PAN
3.Pawłowska S., Highly deformable nanofilaments in flow, JOURNAL OF PHYSICS: CONFERENCE SERIES, ISSN: 1742-6588, DOI: 10.1088/1742-6596/760/1/012022, Vol.760, pp.1-10, 2016
Abstract:

Experimental analysis of hydrogel nanofilaments conveyed by flow is conducted to help in understanding physical phenomena responsible for transport properties and shape deformations of long bio-objects, like DNA or proteins. Investigated hydrogel nanofilaments exhibit typical macromolecules-like behavior, as spontaneous conformational changes and cross-flow migration. Results of the experiments indicate critical role of thermal fluctuations behavior of single filaments.

Affiliations:
Pawłowska S.-IPPT PAN

Conference abstracts
1.Pawłowska S., Zembrzycki K., Kowalewski T.A., Pierini F., Micro and nano-object interaction analysis with femtonewton resolution by Atomic Force Microscopy and Optical Tweezers hybrid system, AFM Bio Med Conference, 2019-09-02/09-06, Munster (DE), pp.65-65, 2019
Abstract:

Gaining knowledge of the solid-solid interactions and hydrodynamic and mechanical properties is crucial for understanding the processes and dynamics of molecular interactions, biological and nano- structures and also to find their future applications.
Atomic force microscopy (AFM) is a versatile technique for nanoscale imaging purposes and for quantify analysis of force at the nanonewton scale. Unfortunately, due to technical limitations and restrictions related to the mechanical properties of cantilevers, this technique cannot detect small forces on the femtonewton scale and analyse the stiffness of very soft materials such as biological tissues or hydrogels. AFM is also use to manipulate materials, however, AFM-based manipulation systems are slow and imprecise. To distinguish, Optical Tweezers (OT) are scientific instruments that can trap small particles and manipulate nano- and micro-materials with much higher precision. The AFM / OT hybrid system is a high-resolution imaging instrument with a lower force limit of detection. It is capable of non-invasively manipulating of nanomaterials, single molecules and living cells, measuring forces with femtonewton accuracy, detecting motion with nanometer (10-9 m) precision and to manipulate objects, but also to obtain images directly in the same sample. The combination of AFM with Optical Tweezers will provide significant advances in biophysical research and in the study of the mechanical properties of nanomaterials [1]. In our system we combine Optical Tweezers with commercial AFM to create an instrument capable of working in hybrid mode. It allows simultaneous manipulation of biological systems of greater complexity and the analysis of their properties. Performed by us, experiments showed that AFM/OT system is a unique technique for visualization of the analysed materials, trapping single micro-objects and measure the interactions (in the range of femtonewton) between single particles. The results obtained by AFM/OT confirm that this equipment is a very useful technique also for determination the mechanical properties of very soft materials (e.g. hydrogels) [2].

Affiliations:
Pawłowska S.-IPPT PAN
Zembrzycki K.-IPPT PAN
Kowalewski T.A.-IPPT PAN
Pierini F.-IPPT PAN
2.Nakielski P., Urbanek O., Pawłowska S., Kowalewski T.A., Pierini F., Externally triggered on-demand drug release from stimuli-responsive hydrogel-based electrospun nanofibers and their composites, Electrospin 2019, 6th International Conference on Electrospinning 2019 , 2019-06-19/06-21, Shanghai (CN), pp.63-63, 2019
Abstract:

Pulsatile drug delivery systems are gaining a lot of interest because of their numerous advantages, especially when compared to conventional pharmaceutical dosage forms [1]. These materials are time- and site-specific drug delivery systems which can minimize deleterious side effects of conventional drug administration systems. Nevertheless, the delivery systems that are of particular interest are the ones with reversible on-off switching capability, because they allow the delivery of therapeutic agents at the proper time after a predetermined lag time. Among the polymers used for biomedical applications, hydrogels are a class of materials of particular significance, because they can provide spatial and temporal control over the release of various types of drugs. Stimuli-responsive hydrogels can release drugs on-demand with a fast release rate through different mechanisms. The effectiveness of this process can be maximized using nanostructured materials with a large surface-area-to-volume ratio such as electrospun nanofibers. Current challenges in the development of hydrogel electrospun fibrous nanomaterials lie in the lack of spinnability of pure hydrogel precursor solutions. Addressing this issue, we firstly designed a new core-shell nanofibrous material in which the poly(N-isopropylacrylamide)-derivative hydrogel is confined within a shell of a spinnable polymer (Figure 1a). Alternatively, we developed a scaffold material in which electrospun nanofibers loaded with different bioactive molecules where surrounded by a stimuli-responsive hydrogel (Figure 1b). Morphological and chemical characterization as well as drug release studies were carried out to confirm the material’s ability to supply different doses of drugs on demand and to study the release mechanism.

Affiliations:
Nakielski P.-IPPT PAN
Urbanek O.-IPPT PAN
Pawłowska S.-IPPT PAN
Kowalewski T.A.-IPPT PAN
Pierini F.-IPPT PAN
3.Nakielski P., Pawłowska S., Urbanek-Świderska O., Woźniak-Jezierska K., Barczewska M., Maksymowicz W., Injectable scaffolds for tissue engineering, ISSCR 2019, International Society for Stem Cell Research Annual Meeting, 2019-06-26/06-29, Los Angeles (US), pp.277-277, 2019
Abstract:

Intervertebral disc diseases are a significant medical problem affecting many people around the world. In Poland, the statistics of the Social Insurance Institution (Medical Abuse in 2016) indicate that low back pains and other intervertebral disc diseases constitute 17% of the total number of days of sick leave. In connection with the above, current work describes design of a composite scaffold as a carrier in cell therapy, which will contribute to the regeneration of the intervertebral disc, including the increase of its height. Our composite scaffold include nanofibers that were prepared with the use of the electrospinning method. This method is a simple but powerful technique for fabricating desirable nano- and microfibers by using a high potential electric field. Human Mesenchymal stem cells (MSCs) were cultured on the scaffold from poly(L-lactide). Proliferation kits and fluorescence microscopy were used to asses cells’ viability and adherence to the nanofibers’ surface. hMSCs were efficiently cultured on the nanofibrous scaffold. Cells could be readily detected in porous structure of the scaffold after 7 and 14 days of culture. Viability and proliferation kits proved that the material is not toxic. Drug release from nanofibrous material of model growth factor was conducted with pharmacopeia protocols. Drug release of the 14 kDa growth factor was achieved for 14 days without burst release. Nanofibrous biomaterials prove their advances in many tissue engineering applications. Adjustable porosity of the scaffold and the biocompability of biomaterial make it perfect candidate for cells’ scaffold in many medical procedures and also as a drug release carrier. With the use of single nanofibers, such biomaterials can also be readily used in minimally invasive procedures to regenerate IVD.

Keywords:

nanofibers, IVD, MSC

Affiliations:
Nakielski P.-IPPT PAN
Pawłowska S.-IPPT PAN
Urbanek-Świderska O.-IPPT PAN
Woźniak-Jezierska K.-University of Warmia and Mazury in Olsztyn (PL)
Barczewska M.-University of Warmia and Mazury in Olsztyn (PL)
Maksymowicz W.-University of Warmia and Mazury in Olsztyn (PL)
4.Nakielski P., De Sio L., Buda R., Guglielmelli A., Pawlowska S., Urbanek O., Kowalewski T.A., Pierini F., Photo-responsive PNIPAM-Gold Nanorods Hydrogel For Biomedical Applications, NOMA2019, The 14th Mediterranean Workshop and Topical Meeting, 2019-06-02/06-08, Cetraro (IT), pp.80-80, 2019
Abstract:

Stimuli-responsive drug delivery systems are gaining a lot of interest due to their numerous advantages, especially when compared to conventional pharmaceutical dosage forms. One of the examples is photo stimulation that together with nanometer size agents, having high absorption in the near-infrared region, generate heat due to the interaction with light. Stimuli-responsive hydrogels with gold nanorods (AuNRs), that are used as photothermal converters, can aid in releasing drugs on-demand with a fast release rate through different mechanisms. Here we report an easy method for preparing AuNRs encapsulated in a poly(N-isopropylacrylamide) (PNIPAm) hydrogel that release water-soluble drugs due to photo stimulation. PNIPAm-AuNRs demonstrated remote, pulsatile drug release and ex vivo action after irradiation using a NIR laser. Morphological and chemical characterization as well as drug release studies were carried out to confirm the material’s ability to supply different doses of drugs on demand and to study the release mechanism. By combining the photothermal property of AuNRs and thermal-responsive effect of PNIPAm, the hydrogel shows fast thermal/photoresponse, high heating rate, high structural integrity and increased drug release due to phase change mechanism.

Keywords:

drug delivery systems, nanofibers

Affiliations:
Nakielski P.-IPPT PAN
De Sio L.-Sapienza University of Rome (IT)
Buda R.-Institute of Physical Chemistry, Polish Academy of Sciences (PL)
Guglielmelli A.-University of Calabria (IT)
Pawlowska S.-IPPT PAN
Urbanek O.-IPPT PAN
Kowalewski T.A.-IPPT PAN
Pierini F.-IPPT PAN
5.Pierini F., Lanzi M., Nakielski P., Pawłowska S., Urbanek O., Kowalewski T.A., Light-matter interaction in electrospun nanofibers: novel conjugated polymer-based one-dimensional nanostructures for organic solar cell applications, NOMA2019, The 14th Mediterranean Workshop and Topical Meeting, 2019-06-02/06-08, Cetraro (IT), pp.55-55, 2019
Abstract:

Single-material organic solar cells (SMOCs) based on fullerene-grafted polythiophenes are considered promising devices for organic solar cells (OSCs). The main efforts in this field focus on the chemical tailoring of polymer molecules to reduce the side effects of charge recombination. These advances have made it possible to obtain a power conversion efficiency (PCE) close to conventional bulk heterojunction (BHJ) cells. So far, however, SMOCs still show inadequate efficiencies due to ineffective charge transport.
Here we show how SMOC efficiency can be strongly increased by optimizing the supramolecular and nanoscale structure of the active layer, while achieving the highest reported efficiency value (PCE = 5.58%) [1]. The enhanced performance may be attributed to well-packed and properly oriented polymer chains. The hierarchical structure is given by the incorporation of electrospun one-dimensional nanostructures obtained from polymer chain stretching. Our results suggest that the active material optimization obtained by the use of electrospun nanofibers plays a key role in the development of efficient SMOCs.

Affiliations:
Pierini F.-IPPT PAN
Lanzi M.-University of Bologna (IT)
Nakielski P.-IPPT PAN
Pawłowska S.-IPPT PAN
Urbanek O.-IPPT PAN
Kowalewski T.A.-IPPT PAN
6.Pawłowska S., Pierini F., Kowalewski T.A., Highly flexible filaments in an oscillatory microchannel flow, EFMC12, 12th European Fluid Mechanics Conference, 2018-09-09/09-13, Wiedeń (AT), pp.1, 2018
7.Pierini F., Lanzi M., Nakielski P., Pawłowska S., Urbanek O., Kowalewski T.A., Electrospinning of polythiophene with pendant fullerene nanofibers for single-material organic solar cells, Electrospin2018 International Conference, 2018-01-16/01-18, Stellenbosch (ZA), pp.19-20, 2018
8.Pawłowska S., Nakielski P., Pierini F., Electrospun polyacrylamide hydrogel nanofibers: from nanocarriers to stimuli responsive nanomaterials, Electrospin2018 International Conference, 2018-01-16/01-18, Stellenbosch (ZA), pp.39, 2018
9.Pawłowska S., Pierini F., Nakielski P., Piechocka I., Zembrzycki K., Kowalewski T.A., Hydrogel nanofilaments in oscillatory microchannel flow, ISFV 18, 18th International Symposium on Flow Visualization, 2018-06-26/06-29, Zurich (CH), pp.233-235, 2018
10.Pierini F., Nakielski P., Pawłowska S., Piechocka I., Zembrzycki K., Kowalewski T.A., Development and applications of atomic force microscopy combined with optical tweezers (AFM/OT), AFM BioMed, 8th AFM BioMed Conference, 2017-09-04/09-08, Kraków (PL), pp.103, 2017
Abstract:

Atomic force microscopy (AFM) is an evolution of scanning tunnelling microscopy that immediately gained popularity thanks to its ability to analyse nanomaterials. Initially, AFM was developed for nanomaterials imaging purposes, however the development of new features made it the most commonly used tool for studying the biophysical properties of biological samples. On the other hand, atomic force microscopy has limited use for examining sub-piconewton forces. Few techniques have been developed to measure forces below the AFM limit of detection. Among them, optical tweezers (OT) stand out for their high resolution, flexibility, and because they make it possible to accurately manipulate biological samples and carry out biophysics experiments without side effects thanks to their non-invasive properties.
The combination of AFM with other techniques in the last decades has significantly extended its capability. The improvement of the AFM force resolution by developing a hybrid double probe instrument based on the combination of AFM and OT has great potential in cell or molecular biology. [1]
We outline principles of atomic force microscopy combined with optical tweezers (AFM/OT) developed by our team underlying the techniques applied during the design, building and instrument use stages. We describe the experimental procedure for calibration of the system and we prove the achievement of a higher resolution (force: 10 fN – spatial: 0.1 nm – temporal: 10 ns) than the stand alone AFM.
We show the use of the hybrid equipment in a number of different biophysics experiments performed employing both AFM and OT probes. The presented studies include the demonstration of simultaneous high-precision nanomanipulation and imaging, the evaluation of single biomolecule mechanical properties and the single cell membrane activation and probing. Finally, we show the further potential applications of our AFM/OT.

Keywords:

AFM, Optical Tweezers

Affiliations:
Pierini F.-IPPT PAN
Nakielski P.-IPPT PAN
Pawłowska S.-IPPT PAN
Piechocka I.-IPPT PAN
Zembrzycki K.-IPPT PAN
Kowalewski T.A.-IPPT PAN
11.Pawłowska S., Nakielski P., Pierini F., Zembrzycki K., Piechocka I.K., Kowalewski T.A., Tumbling, rotating and coiling of nanofilaments in an oscillating microchannel flow, BioNano6, Biomolecules and Nanostructures 6, 2017-05-10/05-14, Podlesice (PL), No.41E, pp.60, 2017
12.Pawłowska S., Pierini F., Nakielski P., Piechocka I., Zembrzycki K., Kowalewski T.A., Lateral Migration of Highly Deformable Nanofilaments Conveyed by Oscillatory Flow, CNM, 5th Conference on Nano- and Micromechanics, 2017-07-04/07-06, Wrocław (PL), pp.29-31, 2017
Keywords:

thermal fluctuations, lateral migration, flexible filaments

Affiliations:
Pawłowska S.-IPPT PAN
Pierini F.-IPPT PAN
Nakielski P.-IPPT PAN
Piechocka I.-IPPT PAN
Zembrzycki K.-IPPT PAN
Kowalewski T.A.-IPPT PAN
13.Pawłowska S., Kowalewski T.A., Lateral migration of solid spheroidal nanoparticles and highly deformable hydrogel nanofilaments under the influence of oscillatory flow, ExFM2017, Experiments in Fluid Mechanics, 2017-10-23/10-24, Warszawa (PL), pp.1-2, 2017
14.Pawłowska S., Nakielski P., Pierini F., Zembrzycki K., Kowalewski T.A., Mobility of highly deformable nanofilaments, ICTAM XXIV, 24th International Congress of Theoretical and Applied Mechanics, 2016-08-21/08-26, Montréal (CA), pp.1196-1197, 2016
Abstract:

Mobility of hydrogel nanofilaments suspended in liquid is investigated to gain basic knowledge on hydrodynamic interactions biased by Brownian fluctuations. Typical for long macromolecules effects like spontaneous conformational changes and cross-flow migration are observed and evaluated. The collected experimental data can be used to validate assumptions present in numerical models describing intercellular transport of long biomolecules.

Keywords:

persistence length, macromolecules, electrospinning, DNA, Brownian motion

Affiliations:
Pawłowska S.-IPPT PAN
Nakielski P.-IPPT PAN
Pierini F.-IPPT PAN
Zembrzycki K.-IPPT PAN
Kowalewski T.A.-IPPT PAN
15.Pierini F., Nakielski P., Pawłowska S., Zembrzycki K., Kowalewski T.A., Particles double layer evaluation by atomic force microscopy - optical tweezers, ICTAM XXIV, 24th International Congress of Theoretical and Applied Mechanics, 2016-08-21/08-26, Montréal (CA), pp.1204-1205, 2016
Abstract:

Atomic force microscopy (AFM) is the most commonly used method of direct force evaluation, but due to its technical limitations this single probe technique is unable to detect forces with femtonewton resolution. We present the development of a combined atomic force microscopy and optical tweezers (AFM/OT) instrument. The optical tweezers system provides us the ability to manipulate small dielectric objects and to use it as a high spatial and temporal resolution displacement and force sensor in the same AFM scanning zone. We demonstrate the possibility to develop a combined instrument with high potential in nanomechanics, molecules manipulation and biologic al studies. The presented study is aimed to quantify the interaction forces between two single polystyrene particles in the femtonewton scale by using the developed AFM/OT equipment.

Keywords:

optical trap, nanomanipulation, femtonewtons

Affiliations:
Pierini F.-IPPT PAN
Nakielski P.-IPPT PAN
Pawłowska S.-IPPT PAN
Zembrzycki K.-IPPT PAN
Kowalewski T.A.-IPPT PAN
16.Pawłowska S., Nakielski P., Pierini F., Zembrzycki K., Kowalewski T.A., Highly Deformable Hydrogel Nanofilaments in Poiseuille Flow, MNF 2016, 5th Micro and Nano Flows Conference, 2016-09-11/09-14, Milan (IT), pp.50, 2016
Keywords:

Hydrogel Nanofilaments, Bending Dynamics, Poiseuille Flow, Electrospinning

Affiliations:
Pawłowska S.-IPPT PAN
Nakielski P.-IPPT PAN
Pierini F.-IPPT PAN
Zembrzycki K.-IPPT PAN
Kowalewski T.A.-IPPT PAN
17.Pierini F., Zembrzycki K., Nakielski P., Pawłowska S., Kowalewski T.A., Nanomanipulating and sensing single particles interactions with combined atomic force microscopy optical tweezers (AFM/OT), MNF 2016, 5th Micro and Nano Flows Conference, 2016-09-11/09-14, Milan (IT), pp.40-41, 2016
Keywords:

Atomic force microscopy/optical tweezers, Nanomanipulation, Single particles analysis, Interaction force measurement, DLVO theory

Affiliations:
Pierini F.-IPPT PAN
Zembrzycki K.-IPPT PAN
Nakielski P.-IPPT PAN
Pawłowska S.-IPPT PAN
Kowalewski T.A.-IPPT PAN
18.Pawłowska S., Highly deformable nanofilaments in flow, XXII Fluid Mechanics Conference, 2016-09-11/09-14, Słok k/Bełchatowa (PL), pp.79-80, 2016
19.Nakielski P., Pawłowska S., Pierini F., Hejduk P., Zembrzycki K., Kowalewski T.A., Novel hydrogel nanofilaments based on electrospun core-shell fibers, Europhysics Conference Biomolecules and Nanostructures 5, 2015-05-13/05-17, Jaroszowice (PL), Vol.39C, pp.101, 2015
Keywords:

nanofilaments, hydrogel, long molecules flexibility

Affiliations:
Nakielski P.-IPPT PAN
Pawłowska S.-IPPT PAN
Pierini F.-IPPT PAN
Hejduk P.-other affiliation
Zembrzycki K.-IPPT PAN
Kowalewski T.A.-IPPT PAN
20.Pawłowska S., Nakielski P., Pierini F., Zembrzycki K., Kowalewski T.A., Mobility of nanofilaments, Experiments in Fluid Mechanics 2015, 2015-10-26/10-27, Warszawa (PL), pp.1, 2015
Abstract:

We propose a microscale experimental model in form of highly deformable nanofilaments, which permits for precise optical measurements and to evaluate hydrodynamic interactions (mobility). The conducted research includes determination of the mechanical properties of elastic hydrogel nanofilaments obtained by electrospinning that can serve as experimental benchmark to validate theoretical and numerical models describing dynamics of long biological molecules (e.g. proteins, DNA). Nanofilaments mechanical properties are determined by studying their dynamic bending. in shear flow and deformations due to the thermal fluctuations (Brownian motion). These results are compared with AFM nanoindentation measurements. Data obtained from this research project will be a base to crea te biocompatible nanoobjects that can become tools for the regeneration of tissue (e.g. neural tissue).

Keywords:

Biocompatible nanoobjects, highly deformable nanofilaments, regeneration of tissue

Affiliations:
Pawłowska S.-IPPT PAN
Nakielski P.-IPPT PAN
Pierini F.-IPPT PAN
Zembrzycki K.-IPPT PAN
Kowalewski T.A.-IPPT PAN
21.Nakielski P., Pawłowska S., Pierini F., Hejduk P., Zembrzycki K., Kowalewski T.A., Processing and mechanical properties relationships in hydrogel nanofilamets for biological application, ICMCSF, International Conference on Mechanics of Complex Solids and Fluids, 2015-05-17/05-22, Lille (FR), pp.1, 2015
Keywords:

Nanofilaments, hydrogel filaments, nanofibres, long nanoobjects deformability

Affiliations:
Nakielski P.-IPPT PAN
Pawłowska S.-IPPT PAN
Pierini F.-IPPT PAN
Hejduk P.-other affiliation
Zembrzycki K.-IPPT PAN
Kowalewski T.A.-IPPT PAN
22.Pierini F., Nakielski P., Pawłowska S., Zembrzycki K., Kowalewski T.A., Hydrogel nanofilaments via core-shell electrospinning, NanoItaly, Nanotechnology meeting forum for research and market, 2015-09-21/09-24, Roma (IT), pp.1, 2015
Abstract:

Controlled drug delivery systems are used to improve the conventional administration of drugs. One of the main challenges is to synthesize materials able to find a defined target and to release drugs in a controlled manner [1]. Several research tasks have been focused on developing ideal drug delivery systems made by hydrogel due to their unique properties [2]. The present study is based on the idea that soft and flexible nanomaterials can easily travel in crowed environments of body fluids and biological tissues. Modification of their mechanical properties obtained by changing of the cross-linker amount may give us the possibility to tune the material rigidity according to desired application. Here, we describe a novel method based on coaxial electrospinning for obtaining highly flexible hydrogel nanofilaments able to transport and release dedicated molecules. Two different types of hydrogels (poly(N,Nisopropyl acrylamide) and polyacrylamide) with three polymer/cross-linker ratios were produced and deeply studied. The nanofilaments morphology was characterized and the release of bovine serum albumin as a function of time was quantified. Mechanical properties of highly deformable hydrogel nanofilaments were evaluated by bending dynamics and Brownian motion observation techniques. The calculated mechanical properties were compared with data obtained by nanoindention. The results highlight the crucial role of morphology and stiffness on mobility of nanofilaments colloid systems. The information gained are fundamental to design nanoobjects with well-defined chemical and physical behaviour.

Keywords:

Nanofilaments, electrospinning, core-shell method, hydrogel

Affiliations:
Pierini F.-IPPT PAN
Nakielski P.-IPPT PAN
Pawłowska S.-IPPT PAN
Zembrzycki K.-IPPT PAN
Kowalewski T.A.-IPPT PAN
23.Kowalewski T.A., Nakielski P., Pierini F., Zembrzycki K., Pawłowska S., Nanoscale challenges of fluid mechanics, PCM-CMM-2015, 3rd Polish Congress of Mechanics and 21st Computer Methods in Mechanics, 2015-09-08/09-11, Gdańsk (PL), pp.11-16, 2015
Abstract:

In this talk we would like to tackle general question of contemporary fluid dynamics, how far its assumption of a continuous, smooth medium remains useful when size and time scales start to approach molecular ones. The question is not trivial and seems to depend on several additional factors usually minored. For example, when full Navier-Stokes equations are replaced by their linear approximation we are loosing basic characteristics of convective motion, and still we use such approach. Once our fluid becomes granular matter with its own internal properties, proper interpretation of flow interactions with other molecular structures probably needs deeper physics. But still we try to convert such problem to the classical macro/micro scale description. Hence a general question arises, how small does a fluid have to be before it is not a fluid anymore?

Keywords:

microfluidics, nanofluids, Brownian motion, nanofilaments

Affiliations:
Kowalewski T.A.-IPPT PAN
Nakielski P.-IPPT PAN
Pierini F.-IPPT PAN
Zembrzycki K.-IPPT PAN
Pawłowska S.-IPPT PAN
24.Pawłowska S., Nakielski P., Hejduk P., Pierini F., Zembrzycki K., Kowalewski T.A., Brownian motion of nanofibers, KKNM, 4th National Conference on Nano- and Micromechanics, 2014-07-08/07-10, Wrocław (PL), pp.162-163, 2014
Keywords:

electrospinning, flexible nanorods, Brownian motion

Affiliations:
Pawłowska S.-IPPT PAN
Nakielski P.-IPPT PAN
Hejduk P.-other affiliation
Pierini F.-IPPT PAN
Zembrzycki K.-IPPT PAN
Kowalewski T.A.-IPPT PAN
25.Pierini F., Hejduk P., Nakielski P., Pawłowska S., Zembrzycki K., Kowalewski T.A., Study of surface interaction forces in polystyrene colloidal nanoparticles systems, XXI FMC, XXI Fluid Mechanics Conference, 2014-06-15/06-18, Kraków (PL), pp.115, 2014
Keywords:

Nanoparticles, polystyrene beads, surface properties, atomic force microscopy, hydrodynamic properties

Affiliations:
Pierini F.-IPPT PAN
Hejduk P.-other affiliation
Nakielski P.-IPPT PAN
Pawłowska S.-IPPT PAN
Zembrzycki K.-IPPT PAN
Kowalewski T.A.-IPPT PAN
26.Pawłowska S., Hejduk P., Nakielski P., Pierini F., Zembrzycki K., Kowalewski T.A., Analysis of nanoparticles hydrodynamic diameters in Brownian motion, XXI FMC, XXI Fluid Mechanics Conference, 2014-06-15/06-18, Kraków (PL), pp.116, 2014
Keywords:

Nanoparticles, Brownian motion, hydrodynamic diameter

Affiliations:
Pawłowska S.-IPPT PAN
Hejduk P.-other affiliation
Nakielski P.-IPPT PAN
Pierini F.-IPPT PAN
Zembrzycki K.-IPPT PAN
Kowalewski T.A.-IPPT PAN