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Polish Academy of Sciences

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Monika Dolega

Université Grenoble Alpes (FR)

Recent publications
1.  Brun-Cosme-Bruny M., Pernet L., Błoński S., Zaremba D., Fraboulet S., Dolega M.E., Microfluidic system for in vitro epithelial folding and calcium waves induction, STAR Protocols, ISSN: 2666-1667, DOI: 10.1016/j.xpro.2022.101683, Vol.3, No.4, pp.101683-101683, 2022

Abstract:
Epithelial folding is a fundamental process where initially flat monolayers transform into functional 3D structures. This protocol details fabrication steps for a polycarbonate microfluidic platform which enables triggering epithelial folds that recapitulate stereotypical cell shape changes and folding-associated mechanical stresses. We describe the steps for cell seeding to form a monolayer on the chip, and subsequent approach to trigger calcium waves in the epithelial monolayer through local epithelial deformation. Lastly, we outline quantitative analysis steps of the epithelial response.

Affiliations:
Brun-Cosme-Bruny M. - other affiliation
Pernet L. - other affiliation
Błoński S. - IPPT PAN
Zaremba D. - IPPT PAN
Fraboulet S. - other affiliation
Dolega M.E. - Université Grenoble Alpes (FR)
2.  Błoński S., Aureille J., Badawi S., Zaremba D., Pernet L., Grichine A., Fraboulet S., Korczyk P.M., Recho P., Guilluy Ch., Dolega M.E., Direction of epithelial folding defines impact of mechanical forces on epithelial state, Developmental Cell, ISSN: 1534-5807, DOI: 10.1016/j.devcel.2021.11.008, Vol.56, pp.3222-3234, 2021

Abstract:
Cell shape dynamics during development is tightly regulated and coordinated with cell fate determination. Triggered by an interplay between biochemical and mechanical signals, epithelia form complex tissues by undergoing coordinated cell shape changes, but how such spatiotemporal coordination is controlled remains an open question. To dissect biochemical signaling from purely mechanical cues, we developed a microfluidic system that experimentally triggers epithelial folding to recapitulate stereotypic deformations observed in vivo. Using this system, we observe that the apical or basal direction of folding results in strikingly different mechanical states at the fold boundary, where the balance between tissue tension and torque (arising from the imposed curvature) controls the spread of folding-induced calcium waves at a short timescale and induces spatial patterns of gene expression at longer timescales. Our work uncovers that folding-associated gradients of cell shape and their resulting mechanical stresses direct spatially distinct biochemical responses within the monolayer.

Keywords:
epithelial morphogenesis, epithelial folding, tension, calcium waves, microfluidics, RNAseq

Affiliations:
Błoński S. - IPPT PAN
Aureille J. - other affiliation
Badawi S. - other affiliation
Zaremba D. - IPPT PAN
Pernet L. - other affiliation
Grichine A. - other affiliation
Fraboulet S. - other affiliation
Korczyk P.M. - IPPT PAN
Recho P. - other affiliation
Guilluy Ch. - other affiliation
Dolega M.E. - Université Grenoble Alpes (FR)
3.  Korczyk P.M., Dolega M.E., Jakieła S., Jankowski P., Makulska S., Garstecki P., Scaling up the throughput of synthesis and extraction in droplet microfluidic reactors, Journal of Flow Chemistry, ISSN: 2062-249X, DOI: 10.1556/JFC-D-14-00038, Vol.5, No.2, pp.110-118, 2015

Abstract:
Conducting reactions in droplets in microfluidic chips offers several highly attractive characteristics, among others, increased yield and selectivity of chemical syntheses. The use of droplet microfluidic systems in synthetic chemistry is, however, hampered by the intrinsically small throughput of micrometric channels. Here, we verify experimentally the potential to increase throughput via an increase of the scale of the channels.We use the results of these experiments characterizing the processes of (1) generation of droplets, (2) mixing in droplets, (3) inter-phase extraction, and (4) the yield of synthesis of pyrrole, to postulate a number of guidelines for scaling up the throughput of microfluidic droplet systems. In particular, we suggest the rules for maximizing the throughput via an increase of the size of the channels and via parallelization to optimize the throughput of synthesis against the cost of fabrication of the chips and against the kinetic requirements of specific reactions.

Keywords:
flow chemistry, microfluidics, synthesis, emulsions, droplets

Affiliations:
Korczyk P.M. - IPPT PAN
Dolega M.E. - Université Grenoble Alpes (FR)
Jakieła S. - Institute of Physical Chemistry, Polish Academy of Sciences (PL)
Jankowski P. - Institute of Physical Chemistry, Polish Academy of Sciences (PL)
Makulska S. - Institute of Physical Chemistry, Polish Academy of Sciences (PL)
Garstecki P. - Institute of Physical Chemistry, Polish Academy of Sciences (PL)

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