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Recent publications
1.  Pokrywczyńska M., Jundziłł A., Adamowicz J., Kowalczyk T., Warda K., Rasmus M., Buchholz Ł., Krzyżanowska S., Nakielski P., Chmielewski T., Bodnar M., Marszałek A., Dębski R., Frontczak-Baniewicz M.M., Mikułowski G., Nowacki M., Kowalewski T.A., Drewa T., Is the Poly (L- Lactide- Co– Caprolactone) Nanofibrous Membrane Suitable for Urinary Bladder Regeneration?, PLOS ONE, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0105295, Vol.9, No.8, pp.105295-1-12, 2014

Abstract:
The purpose of this study was to compare: a new five-layered poly (L–lactide–co–caprolactone) (PLC) membrane and small intestinal submucosa (SIS) as a control in rat urinary bladder wall regeneration. The five-layered poly (L–lactide–co–caprolactone) membrane was prepared by an electrospinning process. Adipose tissue was harvested from five 8-week old male Wistar rats. Adipose derived stem cells (ADSCs) were seeded in a density of 3×106 cells/cm2 onto PLC membrane and SIS scaffolds, and cultured for 5-7 days in the stem cell culture medium. Twenty male Wistar rats were randomly divided into five equal groups. Augmentation cystoplasty was performed in a previously created dome defect. Groups: (I) PLC+ 3×106ADSCs; (II) SIS+ 3×106ADSCs; (III) PLC; (IV) SIS; (V) control. Cystography was performed after three months. The reconstructed urinary bladders were evaluated in H&E and Masson's trichrome staining. Regeneration of all components of the normal urinary bladder wall was observed in bladders augmented with cell-seeded SIS matrices. The urinary bladders augmented with SIS matrices without cells showed fibrosis and graft contraction. Bladder augmentation with the PLC membrane led to numerous undesirable events including: bladder wall perforation, fistula or diverticula formation, and incorporation of the reconstructed wall into the bladder lumen. The new five-layered poly (L–lactide–co–caprolactone) membrane possesses poorer potential for regenerating the urinary bladder wall compared with SIS scaffold.

Keywords:
urinary bladder regeneration, electrospinning

Affiliations:
Pokrywczyńska M. - other affiliation
Jundziłł A. - other affiliation
Adamowicz J. - Nicolaus Copernicus University (PL)
Kowalczyk T. - IPPT PAN
Warda K. - other affiliation
Rasmus M. - Nicolaus Copernicus University (PL)
Buchholz Ł. - Nicolaus Copernicus University (PL)
Krzyżanowska S. - other affiliation
Nakielski P. - IPPT PAN
Chmielewski T. - IPPT PAN
Bodnar M. - Nicolaus Copernicus University (PL)
Marszałek A. - Nicolaus Copernicus University (PL)
Dębski R. - Nicolaus Copernicus University (PL)
Frontczak-Baniewicz M.M. - Mossakowski Medical Research Centre, Polish Academy of Sciences (PL)
Mikułowski G. - IPPT PAN
Nowacki M. - other affiliation
Kowalewski T.A. - IPPT PAN
Drewa T. - Nicolaus Copernicus University (PL)

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