Institute of Fundamental Technological Research
Polish Academy of Sciences

Staff

Chiara Rinoldi, PhD

Department of Biosystems and Soft Matter (ZBiMM)
Division of Modelling in Biology and Medicine (PMBM)
position: Assistant Professor
telephone: (+48) 22 826 12 81 ext.: 330
room: 320
e-mail:
ORCID: 0000-0002-4028-375X

Doctoral thesis
2020-09-08 Spun fiber-based scaffolds for tendon tissue engineering  (Politechnika Warszawska)
supervisor -- Wojciech Święszkowski, PhD, DSc, PW
co-supervisor -- Ewa Kijeńska-Gawrońska, PhD, PW
 

Recent publications
1.  Ziai Y., Zargarian Seyed S., Rinoldi C., Nakielski P., Sola A., Lanzi M., Truong Yen B., Pierini F., Conducting polymer-based nanostructured materials for brain–machine interfaces, WIREs Nanomedicine and Nanobiotechnology, ISSN: 1939-0041, DOI: 10.1002/wnan.1895, pp.e1895-1-33, 2023

Abstract:
As scientists discovered that raw neurological signals could translate into bioelectric information, brain–machine interfaces (BMI) for experimental and clinical studies have experienced massive growth. Developing suitable materials for bioelectronic devices to be used for real-time recording and data digitalizing has three important necessitates which should be covered. Biocompatibility, electrical conductivity, and having mechanical properties similar to soft brain tissue to decrease mechanical mismatch should be adopted for all materials. In this review, inorganic nanoparticles and intrinsically conducting polymers are discussed to impart electrical conductivity to systems, where soft materials such as hydrogels can offer reliable mechanical properties and a biocompatible substrate. Interpenetrating hydrogel networks offer more mechanical stability and provide a path for incorporating polymers with desired properties into one strong network. Promising fabrication methods, like electrospinning and additive manufacturing, allow scientists to customize designs for each application and reach the maximum potential for the system. In the near future, it is desired to fabricate biohybrid conducting polymer-based interfaces loaded with cells, giving the opportunity for simultaneous stimulation and regeneration. Developing multi-modal BMIs, Using artificial intelligence and machine learning to design advanced materials are among the future goals for this field.

Keywords:
3D printing, brain–machine interface, conductive hydrogels, electrospinning, neural recording

Affiliations:
Ziai Y. - IPPT PAN
Zargarian Seyed S. - IPPT PAN
Rinoldi C. - IPPT PAN
Nakielski P. - IPPT PAN
Sola A. - other affiliation
Lanzi M. - University of Bologna (IT)
Truong Yen B. - other affiliation
Pierini F. - IPPT PAN
2.  Rybak D., Su Y., Li Y., Ding B., Lv X., Li Z., Yeh Y., Nakielski P., Rinoldi C., Pierini F., Dodda J. M., Evolution of nanostructured skin patches towards multifunctional wearable platforms for biomedical applications, NANOSCALE, ISSN: 2040-3364, DOI: 10.1039/D3NR00807J, pp.1-40, 2023

Abstract:
Recent advances in the field of skin patches have promoted the development of wearable and implantable bioelectronics for long-term, continuous healthcare management and targeted therapy. However, the design of electronic skin (e-skin) patches with stretchable components is still challenging and requires an in-depth understanding of the skin-attachable substrate layer, functional biomaterials and advanced self-powered electronics. In this comprehensive review, we present the evolution of skin patches from functional nanostructured materials to multi-functional and stimuli-responsive patches towards flexible substrates and emerging biomaterials for e-skin patches, including the material selection, structure design and promising applications. Stretchable sensors and self-powered e-skin patches are also discussed, ranging from electrical stimulation for clinical procedures to continuous health monitoring and integrated systems for comprehensive healthcare management. Moreover, an integrated energy harvester with bioelectronics enables the fabrication of self-powered electronic skin patches, which can effectively solve the energy supply and overcome the drawbacks induced by bulky battery-driven devices. However, to realize the full potential offered by these advancements, several challenges must be addressed for next-generation e-skin patches. Finally, future opportunities and positive outlooks are presented on the future directions of bioelectronics. It is believed that innovative material design, structure engineering, and in-depth study of fundamental principles can foster the rapid evolution of electronic skin patches, and eventually enable self-powered close-looped bioelectronic systems to benefit mankind.

Affiliations:
Rybak D. - IPPT PAN
Su Y. - other affiliation
Li Y. - other affiliation
Ding B. - Donghua University (CN)
Lv X. - other affiliation
Li Z. - other affiliation
Yeh Y. - other affiliation
Nakielski P. - IPPT PAN
Rinoldi C. - IPPT PAN
Pierini F. - IPPT PAN
Dodda J. M. - other affiliation
3.  Haghighat Bayan Mohammad A., Dias Yasmin J., Rinoldi C., Nakielski P., Rybak D., Truong Yen B., Yarin A., Pierini F., Near-infrared light activated core-shell electrospun nanofibers decorated with photoactive plasmonic nanoparticles for on-demand smart drug delivery applications, Journal of Polymer Science, ISSN: 2642-4169, DOI: 10.1002/pol.20220747, pp.1-13, 2023

Abstract:
Over the last few years, traditional drug delivery systems (DDSs) have been transformed into smart DDSs. Recent advancements in biomedical nanotech-nology resulted in introducing stimuli-responsiveness to drug vehicles. Nano-
platforms can enhance drug release efficacy while reducing the side effects of drugs by taking advantage of the responses to specific internal or external stim-uli. In this study, we developed an electrospun nanofibrous photo-responsive DDSs. The photo-responsivity of the platform enables on-demand elevated drug release. Furthermore, it can provide a sustained release profile and pre-vent burst release and high concentrations of drugs. A coaxial electrospinning setup paired with an electrospraying technique is used to fabricate core-shell PVA-PLGA nanofibers decorated with plasmonic nanoparticles. The fabricated
nanofibers have a hydrophilic PVA and Rhodamine-B (RhB) core, while the shell is hydrophobic PLGA decorated with gold nanorods (Au NRs). The presence of plasmonic nanoparticles enables the platform to twice the amount of drug release besides exhibiting a long-term release. Investigations into the photo-responsive release mechanism demonstrate the system's potential as a “smart” drug delivery platform.

Keywords:
electrospun core-shell nanofibers,NIR-light activation,on-demand drug release,plasmonic nanoparticles,stimuli-responsive nanomaterials

Affiliations:
Haghighat Bayan Mohammad A. - IPPT PAN
Dias Yasmin J. - other affiliation
Rinoldi C. - IPPT PAN
Nakielski P. - IPPT PAN
Rybak D. - IPPT PAN
Truong Yen B. - other affiliation
Yarin A. - Technion-Israel Institute of Technology (IL)
Pierini F. - IPPT PAN
4.  Rinoldi C., Ziai Y., Zargarian Seyed S., Nakielski P., Zembrzycki K., Haghighat Bayan Mohammad A., Zakrzewska A., Fiorelli R., Lanzi M., Kostrzewska-Księżyk A., Czajkowski R., Kublik E., Kaczmarek L., Pierini F., In Vivo Chronic Brain Cortex Signal Recording Based on a Soft Conductive Hydrogel Biointerface, ACS Applied Materials and Interfaces, ISSN: 1944-8244, DOI: 10.1021/acsami.2c17025, pp.A-N, 2022

Abstract:
In neuroscience, the acquisition of neural signals from the brain cortex is crucial to analyze brain processes, detect neurological disorders, and offer therapeutic brain–computer interfaces. The design of neural interfaces conformable to the brain tissue is one of today’s major challenges since the insufficient biocompatibility of those systems provokes a fibrotic encapsulation response, leading to an inaccurate signal recording and tissue damage precluding long-term/permanent implants. The design and production of a novel soft neural biointerface made of polyacrylamide hydrogels loaded with plasmonic silver nanocubes are reported herein. Hydrogels are surrounded by a silicon-based template as a supporting element for guaranteeing an intimate neural-hydrogel contact while making possible stable recordings from specific sites in the brain cortex. The nanostructured hydrogels show superior electroconductivity while mimicking the mechanical characteristics of the brain tissue. Furthermore, in vitro biological tests performed by culturing neural progenitor cells demonstrate the biocompatibility of hydrogels along with neuronal differentiation. In vivo chronic neuroinflammation tests on a mouse model show no adverse immune response toward the nanostructured hydrogel-based neural interface. Additionally, electrocorticography acquisitions indicate that the proposed platform permits long-term efficient recordings of neural signals, revealing the suitability of the system as a chronic neural biointerface.

Keywords:
brain−machine interface,conductive hydrogels,nanostructured biomaterials,in vitro and in vivo biocompatibility,long-term neural recording

Affiliations:
Rinoldi C. - IPPT PAN
Ziai Y. - IPPT PAN
Zargarian Seyed S. - IPPT PAN
Nakielski P. - IPPT PAN
Zembrzycki K. - IPPT PAN
Haghighat Bayan Mohammad A. - IPPT PAN
Zakrzewska A. - IPPT PAN
Fiorelli R. - IPPT PAN
Lanzi M. - University of Bologna (IT)
Kostrzewska-Księżyk A. - other affiliation
Czajkowski R. - other affiliation
Kublik E. - other affiliation
Kaczmarek L. - other affiliation
Pierini F. - IPPT PAN
5.  Nakielski P., Rinoldi C., Pruchniewski M., Pawłowska S., Gazińska M., Strojny B., Rybak D., Jezierska-Woźniak K., Urbanek O., Denis P., Sinderewicz E., Czelejewska W., Staszkiewicz-Chodor J., Grodzik M., Ziai Y., Barczewska M., Maksymowicz W., Pierini F., Laser-assisted fabrication of injectable nanofibrous cell carriers, Small, ISSN: 1613-6810, DOI: 10.1002/smll.202104971, Vol.18, No.2, pp.2104971-1-18, 2022

Abstract:
The use of injectable biomaterials for cell delivery is a rapidly expanding field which may revolutionize the medical treatments by making them less invasive. However, creating desirable cell carriers poses significant challenges to the clinical implementation of cell-based therapeutics. At the same time, no method has been developed to produce injectable microscaffolds (MSs) from electrospun materials. Here the fabrication of injectable electrospun nanofibers is reported on, which retain their fibrous structure to mimic the extracellular matrix. The laser-assisted micro-scaffold fabrication has produced tens of thousands of MSs in a short time. An efficient attachment of cells to the surface and their proliferation is observed, creating cell-populated MSs. The cytocompatibility assays proved their biocompatibility, safety, and potential as cell carriers. Ex vivo results with the use of bone and cartilage tissues proved that NaOH hydrolyzed and chitosan functionalized MSs are compatible with living tissues and readily populated with cells. Injectability studies of MSs showed a high injectability rate, while at the same time, the force needed to eject the load is no higher than 25 N. In the future, the produced MSs may be studied more in-depth as cell carriers in minimally invasive cell therapies and 3D bioprinting applications.

Affiliations:
Nakielski P. - IPPT PAN
Rinoldi C. - IPPT PAN
Pruchniewski M. - other affiliation
Pawłowska S. - IPPT PAN
Gazińska M. - other affiliation
Strojny B. - other affiliation
Rybak D. - IPPT PAN
Jezierska-Woźniak K. - other affiliation
Urbanek O. - IPPT PAN
Denis P. - IPPT PAN
Sinderewicz E. - other affiliation
Czelejewska W. - other affiliation
Staszkiewicz-Chodor J. - other affiliation
Grodzik M. - other affiliation
Ziai Y. - IPPT PAN
Barczewska M. - University of Warmia and Mazury in Olsztyn (PL)
Maksymowicz W. - University of Warmia and Mazury in Olsztyn (PL)
Pierini F. - IPPT PAN
6.  Paradiso Alessia ., Volpi M., Rinoldi C., Celikkin N., Contessi Negrini N., Bilgen M., Dallera G., Pierini F., Costantini M., Święszkowski Wojciech ., Farè S., In vitro functional models for human liver diseases and drug screening: beyond animal testing, Biomaterials Science, ISSN: 2047-4849, DOI: 10.1039/d1bm01872h, pp.1-28, 2022
7.  Ziai Y., Petronella F., Rinoldi C., Nakielski P., Zakrzewska A., Kowalewski T.A., Augustyniak W., Li X., Calogero A., Sabała I., Ding B., De Sio L., Pierini F., Chameleon-inspired multifunctional plasmonic nanoplatforms for biosensing applications, NPG Asia Materials, ISSN: 1884-4049, DOI: 10.1038/s41427-022-00365-9, Vol.14, pp.18-1-17, 2022

Abstract:
One of the most fascinating areas in the field of smart biopolymers is biomolecule sensing. Accordingly, multifunctional biomimetic, biocompatible, and stimuli-responsive materials based on hydrogels have attracted much interest. Within this framework, the design of nanostructured materials that do not require any external energy source is beneficial for developing a platform for sensing glucose in body fluids. In this article, we report the realization and application of an innovative platform consisting of two outer layers of a nanocomposite plasmonic hydrogel plus one inner layer of electrospun mat fabricated by electrospinning, where the outer layers exploit photoinitiated free radical polymerization, obtaining a compact and stable device. Inspired by the exceptional features of chameleon skin, plasmonic silver nanocubes are embedded into a poly(N-isopropylacrylamide)-based hydrogel network to obtain enhanced thermoresponsive and antibacterial properties. The introduction of an electrospun mat creates a compatible environment for the homogeneous hydrogel coating while imparting excellent mechanical and structural properties to the final system. Chemical, morphological, and optical characterizations were performed to investigate the structure of the layers and the multifunctional platform. The synergetic effect of the nanostructured system’s photothermal responsivity and antibacterial properties was evaluated. The sensing features associated with the optical properties of silver nanocubes revealed that the proposed multifunctional system is a promising candidate for glucose-sensing applications.

Affiliations:
Ziai Y. - IPPT PAN
Petronella F. - other affiliation
Rinoldi C. - IPPT PAN
Nakielski P. - IPPT PAN
Zakrzewska A. - IPPT PAN
Kowalewski T.A. - IPPT PAN
Augustyniak W. - Mossakowski Medical Research Centre, Polish Academy of Sciences (PL)
Li X. - Donghua University (CN)
Calogero A. - Sapienza University of Rome (IT)
Sabała I. - Mossakowski Medical Research Centre, Polish Academy of Sciences (PL)
Ding B. - Donghua University (CN)
De Sio L. - Sapienza University of Rome (IT)
Pierini F. - IPPT PAN
8.  Quint J.P., Samandari M., Abbasi L., Mollocana E., Rinoldi C., Mostafavic A., Tamayol A., Nanoengineered myogenic scaffolds for skeletal muscle tissue engineering, NANOSCALE, ISSN: 2040-3364, DOI: 10.1039/D1NR06143G, Vol.14, pp.797-814, 2022

Abstract:
Extreme loss of skeletal muscle overwhelms the natural regenerative capability of the body, results in permanent disability and substantial economic burden. Current surgical techniques result in poor healing, secondary injury to the autograft donor site, and incomplete recuperation of muscle function. Most current tissue engineering and regenerative strategies fail to create an adequate mechanical and biological environment that enables cell infiltration, proliferation, and myogenic differentiation. In this study, we present a nanoengineered skeletal muscle scaffold based on functionalized gelatin methacrylate (GelMA) hydrogel, optimized for muscle progenitors’ proliferation and differentiation. The scaffold was capable of controlling the release of insulin-like growth factor 1 (IGF-1), an important myogenic growth factor, by utilizing the electrostatic interactions with LAPONITE® nanoclays (NCs). Physiologically relevant levels of IGF-1 were maintained during a controlled release over two weeks. The NC was able to retain 50% of the released IGF-1 within the hydrogel niche, significantly improving cellular proliferation and differentiation compared to control hydrogels. IGF-1 supplemented medium controls required 44% more IGF-1 than the comparable NC hydrogel composites. The nanofunctionalized scaffold is a viable option for the treatment of extreme muscle injuries and offers scalable benefits for translational interventions and the growing field of clean meat production.

Affiliations:
Quint J.P. - University of Connecticut (US)
Samandari M. - University of Connecticut (US)
Abbasi L. - The City College of New York (US)
Mollocana E. - University of Nebraska (US)
Rinoldi C. - IPPT PAN
Mostafavic A. - University of Nebraska (US)
Tamayol A. - Massachusetts Institute of Technology (US)
9.  Liguori A., Pandini S., Rinoldi C., Zaccheroni N., Pierini F., Focarete M.L., Gualandi C., Thermoactive smart electrospun nanofibers, Macromolecular Rapid Communications, ISSN: 1022-1336, DOI: 10.1002/marc.202100694, Vol.43, No.5, pp.2100694-1-35, 2022

Abstract:
The recent burst of research on smart materials is a clear evidence of the growing interest of the scientific community, industry, and society in the field. The exploitation of the great potential of stimuli-responsive materials for sensing, actuation, logic, and control applications is favored and supported by new manufacturing technologies, such as electrospinning, that allows to endow smart materials with micro- and nanostructuration, thus opening up additional and unprecedented prospects. In this wide and lively scenario, this article systematically reviews the current advances in the development of thermoactive electrospun fibers and textiles, sorting them, according to their response to the thermal stimulus. Hence, several platforms including thermoresponsive systems, shape memory polymers, thermo-optically responsive systems, phase change materials, thermoelectric materials, and pyroelectric materials, are described and critically discussed. The difference in active species and outputs of the aforementioned categories is highlighted, evidencing the transversal nature of temperature stimulus. Moreover, the potential of novel thermoactive materials are pointed out, revealing how their development could take to utmost interesting achievements.

Keywords:
electrospinning, phase change materials, pyroelectric materials, shape memory polymers, thermoelectric materials, thermo-optically responsive materials, thermoresponsive materials

Affiliations:
Liguori A. - University of Bologna (IT)
Pandini S. - University of Brescia (IT)
Rinoldi C. - IPPT PAN
Zaccheroni N. - University of Bologna (IT)
Pierini F. - IPPT PAN
Focarete M.L. - University of Bologna (IT)
Gualandi C. - University of Bologna (IT)
10.  Ziai Y., Rinoldi C., Nakielski P., De Sio L., Pierini F., Smart plasmonic hydrogels based on gold and silver nanoparticles for biosensing application, Current Opinion in Biomedical Engineering, ISSN: 2468-4511, DOI: 10.1016/j.cobme.2022.100413, Vol.24, pp.100413-1-8, 2022

Abstract:
The importance of having a fast, accurate, and reusable track for detection has led to an increase investigation in the field of biosensing. Optical biosensing using plasmonic nanoparticles, such as gold and silver, introduces localized surface plasmon resonance (LSPR) sensors. LSPR biosensors are progressive in their sensing precision and detection limit. Also, the possibility to tune the sensing range by varying the size and shape of the particles has made them extremely useful. Hydrogels being hydrophilic 3D networks can be beneficial when used as matrices, because of a more efficient biorecognition. Stimuli-responsive hydrogels can be great candidates, as their response to a stimulus can increase recognition and detection. This article highlights recent advances in combining hydrogels as a matrix and plasmonic nanoparticles as sensing elements. The end capability and diversity of these novel biosensors in different applications in the near future are discussed.

Keywords:
Smart materials, Plasmonic hydrogel, Biosensing

Affiliations:
Ziai Y. - IPPT PAN
Rinoldi C. - IPPT PAN
Nakielski P. - IPPT PAN
De Sio L. - Sapienza University of Rome (IT)
Pierini F. - IPPT PAN
11.  Rinoldi C., Kijeńska-Gawrońska E., Khademhosseini A., Tamayol A., Swieszkowski W., Fibrous systems as potential solutions for tendon and ligament repair, healing, and regeneration, ADVANCED HEALTHCARE MATERIALS, ISSN: 2192-2659, DOI: 10.1002/adhm.202001305, Vol.10, No.7, pp.2001305 - 1-26, 2021
12.  Rinoldi C., Lanzi M., Fiorelli R., Nakielski P., Zembrzycki K., Kowalewski T., Urbanek O., Jezierska-Woźniak K., Maksymowicz W., Camposeo A., Bilewicz R., Pisignano D., Sanai N., Pierini F., Pierini F., Three-dimensional printable conductive semi-interpenetrating polymer network hydrogel for neural tissue applications, BIOMACROMOLECULES, ISSN: 1525-7797, DOI: 10.1021/acs.biomac.1c00524, Vol.22, No.7, pp.3084-3098, 2021

Abstract:
Intrinsically conducting polymers (ICPs) are widely used to fabricate biomaterials; their application in neural tissue engineering, however, is severely limited because of their hydrophobicity and insufficient mechanical properties. For these reasons, soft conductive polymer hydrogels (CPHs) are recently developed, resulting in a water-based system with tissue-like mechanical, biological, and electrical properties. The strategy of incorporating ICPs as a conductive component into CPHs is recently explored by synthesizing the hydrogel around ICP chains, thus forming a semi-interpenetrating polymer network (semi-IPN). In this work, a novel conductive semi-IPN hydrogel is designed and synthesized. The hybrid hydrogel is based on a poly(N-isopropylacrylamide-co-N-isopropylmethacrylamide) hydrogel where polythiophene is introduced as an ICP to provide the system with good electrical properties. The fabrication of the hybrid hydrogel in an aqueous medium is made possible by modifying and synthesizing the monomers of polythiophene to ensure water solubility. The morphological, chemical, thermal, electrical, electrochemical, and mechanical properties of semi-IPNs were fully investigated. Additionally, the biological response of neural progenitor cells and mesenchymal stem cells in contact with the conductive semi-IPN was evaluated in terms of neural differentiation and proliferation. Lastly, the potential of the hydrogel solution as a 3D printing ink was evaluated through the 3D laser printing method. The presented results revealed that the proposed 3D printable conductive semi-IPN system is a good candidate as a scaffold for neural tissue applications.

Affiliations:
Rinoldi C. - IPPT PAN
Lanzi M. - University of Bologna (IT)
Fiorelli R. - other affiliation
Nakielski P. - IPPT PAN
Zembrzycki K. - IPPT PAN
Kowalewski T. - IPPT PAN
Grippo V. - other affiliation
Urbanek O. - IPPT PAN
Jezierska-Woźniak K. - other affiliation
Maksymowicz W. - University of Warmia and Mazury in Olsztyn (PL)
Camposeo A. - other affiliation
Bilewicz R. - other affiliation
Pisignano D. - other affiliation
Sanai N. - other affiliation
Pierini F. - IPPT PAN
13.  Rinoldi C., Zargarian S.S., Nakielski P., Li X., Liguori A., Petronella F., Presutti D., Wang Q., Costantini M., De Sio L., Gualandi C., Ding B., Pierini F., Nanotechnology-assisted RNA delivery: from nucleic acid therapeutics to COVID-19 vaccines, Small Methods, ISSN: 2366-9608, DOI: 10.1002/smtd.202100402, Vol.5, No.9, pp.2100402-1-49, 2021

Abstract:
In recent years, the main quest of science has been the pioneering of the groundbreaking biomedical strategies needed for achieving a personalized medicine. Ribonucleic acids (RNAs) are outstanding bioactive macromolecules identified as pivotal actors in regulating a wide range of biochemical pathways. The ability to intimately control the cell fate and tissue activities makes RNA-based drugs the most fascinating family of bioactive agents. However, achieving a widespread application of RNA therapeutics in humans is still a challenging feat, due to both the instability of naked RNA and the presence of biological barriers aimed at hindering the entrance of RNA into cells. Recently, material scientists’ enormous efforts have led to the development of various classes of nanostructured carriers customized to overcome these limitations. This work systematically reviews the current advances in developing the next generation of drugs based on nanotechnology-assisted RNA delivery. The features of the most used RNA molecules are presented, together with the development strategies and properties of nanostructured vehicles. Also provided is an in-depth overview of various therapeutic applications of the presented systems, including coronavirus disease vaccines and the newest trends in the field. Lastly, emerging challenges and future perspectives for nanotechnology-mediated RNA therapies are discussed.

Affiliations:
Rinoldi C. - IPPT PAN
Zargarian S.S. - IPPT PAN
Nakielski P. - IPPT PAN
Li X. - Donghua University (CN)
Liguori A. - University of Bologna (IT)
Petronella F. - other affiliation
Presutti D. - Institute of Physical Chemistry, Polish Academy of Sciences (PL)
Wang Q. - Donghua University (CN)
Costantini M. - Sapienza University of Rome (IT)
De Sio L. - Sapienza University of Rome (IT)
Gualandi C. - University of Bologna (IT)
Ding B. - Donghua University (CN)
Pierini F. - IPPT PAN
14.  Nakielski P., Pawłowska S., Rinoldi C., Ziai Y., De Sio L., Urbanek O., Zembrzycki K., Pruchniewski M., Lanzi M., Salatelli E., Calogero A., Kowalewski T.A., Yarin A.L., Pierini F., Multifunctional platform based on electrospun nanofibers and plasmonic hydrogel: a smart nanostructured pillow for near-Infrared light-driven biomedical applications, ACS Applied Materials and Interfaces, ISSN: 1944-8244, DOI: 10.1021/acsami.0c13266, Vol.12, No.49, pp.54328-54342, 2020

Abstract:
Multifunctional nanomaterials with the ability torespond to near-infrared (NIR) light stimulation are vital for thedevelopment of highly efficient biomedical nanoplatforms with apolytherapeutic approach. Inspired by the mesoglea structure ofjellyfish bells, a biomimetic multifunctional nanostructured pillowwith fast photothermal responsiveness for NIR light-controlled on-demand drug delivery is developed. We fabricate a nanoplatformwith several hierarchical levels designed to generate a series ofcontrolled, rapid, and reversible cascade-like structural changesupon NIR light irradiation. The mechanical contraction of thenanostructured platform, resulting from the increase of temper-ature to 42°C due to plasmonic hydrogel−light interaction, causesa rapid expulsion of water from the inner structure, passing through an electrospun membrane anchored onto the hydrogel core. Themutual effects of the rise in temperature and waterflow stimulate the release of molecules from the nanofibers. To expand thepotential applications of the biomimetic platform, the photothermal responsiveness to reach the typical temperature level forperforming photothermal therapy (PTT) is designed. The on-demand drug model penetration into pig tissue demonstrates theefficiency of the nanostructured platform in the rapid and controlled release of molecules, while the high biocompatibility confirmsthe pillow potential for biomedical applications based on the NIR light-driven multitherapy strategy.

Keywords:
bioinspired materials, NIR-light responsive nanomaterials, multifunctional platforms, electrospun nanofibers, plasmonic hydrogel, photothermal-based polytherapy, on-demand drug delivery

Affiliations:
Nakielski P. - IPPT PAN
Pawłowska S. - IPPT PAN
Rinoldi C. - IPPT PAN
Ziai Y. - IPPT PAN
De Sio L. - Sapienza University of Rome (IT)
Urbanek O. - IPPT PAN
Zembrzycki K. - IPPT PAN
Pruchniewski M. - other affiliation
Lanzi M. - University of Bologna (IT)
Salatelli E. - University of Bologna (IT)
Calogero A. - Sapienza University of Rome (IT)
Kowalewski T.A. - IPPT PAN
Yarin A.L. - Technion-Israel Institute of Technology (IL)
Pierini F. - IPPT PAN
15.  Fallahi A., Yazdi I., Serex L., Lasha E., Faramarzi N., Tarlan F., Avci H., Almeida R., Sharifi F., Rinoldi C., Gomes M.E., Shin S.R., Khademhosseini A., Akbari M., Tamayol A., Customizable composite fibers for engineering skeletal muscle models, ACS BIOMATERIALS SCIENCE & ENGINEERING, ISSN: 2373-9878, DOI: 10.1021/acsbiomaterials.9b00992, Vol.6, No.2, pp.1112-1123, 2020

Abstract:
Engineering tissue-like scaffolds that can mimic the microstructure, architecture, topology, and mechanical properties of native tissues while offering an excellent environment for cellular growth has remained an unmet need. To address these challenges, multi-compartment composite fibers are fabricated. These fibers can be assembled through textile processes to tailor tissue-level mechanical and electrical properties independent of cellular level components. Textile technologies also allow controlling the distribution of different cell types and microstructure of fabricated constructs and directing cellular growth within 3D microenvironment. Here, we engineered composite fibers from biocompatible cores and biologically relevant hydrogel sheaths. The fibers are mechanically robust to be assembled using textile processes and could support adhesion, proliferation and maturation of cell populations important for engineering of skeletal muscles. We also demonstrated that the changes in the electrical conductivity of the multi-compartment fibers could significantly enhance myogenesis in vitro.

Keywords:
reinforced fibers, biotextiles, tissue engineering, organ weaving, interpenetrating network hydrogels, skeletal muscles

Affiliations:
Fallahi A. - Paul Scherrer Institut (CH)
Yazdi I. - Massachusetts Institute of Technology (US)
Serex L. - Brigham and Women's Hospital (US)
Lasha E. - Brigham and Women's Hospital (US)
Faramarzi N. - Brigham and Women's Hospital (US)
Tarlan F. - Brigham and Women's Hospital (US)
Avci H. - Eskisehir Osmangazi University (TR)
Almeida R. - Brigham and Women's Hospital (US)
Sharifi F. - Massachusetts Institute of Technology (US)
Rinoldi C. - other affiliation
Gomes M.E. - University of Minho (PT)
Shin S.R. - Massachusetts Institute of Technology (US)
Khademhosseini A. - Massachusetts Institute of Technology (US)
Akbari M. - Brigham and Women's Hospital (US)
Tamayol A. - Massachusetts Institute of Technology (US)
16.  Pawłowska S., Rinoldi C., Nakielski P., Ziai Y., Urbanek O., Li X., Kowalewski T.A., Ding B., Pierini F., Ultraviolet light‐assisted electrospinning of core–shell fully cross‐linked P(NIPAAm‐co‐NIPMAAm) hydrogel‐based nanofibers for thermally induced drug delivery self‐regulation, Advanced Materials Interfaces, ISSN: 2196-7350, DOI: 10.1002/admi.202000247, Vol.7, No.12, pp.2000247-1-13, 2020

Abstract:
Body tissues and organs have complex functions which undergo intrinsic changes during medical treatments. For the development of ideal drug delivery systems, understanding the biological tissue activities is necessary to be able to design materials capable of changing their properties over time, on the basis of the patient's tissue needs. In this study, a nanofibrous thermal‐responsive drug delivery system is developed. The thermo‐responsivity of the system makes it possible to self‐regulate the release of bioactive molecules, while reducing the drug delivery at early stages, thus avoiding high concentrations of drugs which may be toxic for healthy cells. A co‐axial electrospinning technique is used to fabricate core–shell cross‐linked copolymer poly(N‐isopropylacrylamide‐co‐N‐isopropylmethacrylamide) (P(NIPAAm‐co‐NIPMAAm)) hydrogel‐based nanofibers. The obtained nanofibers are made of a core of thermo‐responsive hydrogel containing a drug model, while the outer shell is made of poly‐l‐lactide‐co‐caprolactone (PLCL). The custom‐made electrospinning apparatus enables the in situ cross‐linking of P(NIPAAm‐co‐NIPMAAm) hydrogel into a nanoscale confined space, which improves the electrospun nanofiber drug dosing process, by reducing its provision and allowing a self‐regulated release control. The mechanism of the temperature‐induced release control is studied in depth, and it is shown that the system is a promising candidate as a "smart" drug delivery platform.

Keywords:
biomimetic nanomaterials, electrospun core–shell nanofibers, hierarchical nanostructures, smart drug delivery, thermo‐responsive hydrogels

Affiliations:
Pawłowska S. - IPPT PAN
Rinoldi C. - IPPT PAN
Nakielski P. - IPPT PAN
Ziai Y. - IPPT PAN
Urbanek O. - IPPT PAN
Li X. - Donghua University (CN)
Kowalewski T.A. - IPPT PAN
Ding B. - Donghua University (CN)
Pierini F. - IPPT PAN
17.  Nasajpour A., Mostafavi A., Chlanda A., Rinoldi C., Sharifi S., Ji M.S., Ye M., Jonas S.J., Święszkowski W., Weiss P.S., Khademhosseini A., Tamayol A., Cholesteryl ester liquid crystal nanofibers for tissue engineering applications, ACS Materials Letters, ISSN: 2639-4979, DOI: 10.1021/acsmaterialslett.0c00224, Vol.2, No.9, pp.1067-1073, 2020

Abstract:
Liquid-crystal-based biomaterials provide promising platforms for the development of dynamic and responsive interfaces for tissue engineering. Cholesteryl ester liquid crystals (CLCs) are particularly well suited for these applications, due to their roles in cellular homeostasis and their intrinsic ability to organize into supramolecular helicoidal structures on the mesoscale. Here, we developed a nonwoven CLC electrospun scaffold by dispersing three cholesteryl ester-based mesogens within polycaprolactone (PCL). We tuned the ratio of our mesogens so that the CLC would be in the mesophase at the cell culture incubator temperature of 37°C. In these scaffolds, the PCL polymer provided an elastic bulk matrix while the homogeneously dispersed CLC established a viscoelastic fluidlike interface. Atomic force microscopy revealed that the 50% (w/v) cholesteryl ester liquid crystal scaffold (CLC-S) exhibited a mesophase with topographic striations typical of liquid crystals. Additionally, the CLC-S favorable wettability and ultrasoft fiber mechanics enhanced cellular attachment and proliferation. Increasing the CLC concentration within the composites enhanced myoblast adhesion strength promoted myofibril formationin vitrowith mouse myoblast cell lines.

Affiliations:
Nasajpour A. - Massachusetts Institute of Technology (US)
Mostafavi A. - other affiliation
Chlanda A. - Warsaw University of Technology (PL)
Rinoldi C. - other affiliation
Sharifi S. - other affiliation
Ji M.S. - other affiliation
Ye M. - other affiliation
Jonas S.J. - other affiliation
Święszkowski W. - other affiliation
Weiss P.S. - other affiliation
Khademhosseini A. - Massachusetts Institute of Technology (US)
Tamayol A. - Massachusetts Institute of Technology (US)
18.  Rinoldi C., Fallahi A., Yazdi I.K., Paras J.C., Kijeńska-Gawrońska E., Trujillo-de Santiago G., Tuoheti A., Demarchi D., Annabi N., Khademhosseini A., Święszkowski W., Tamayol A., Mechanical and biochemical stimulation of 3D multilayered scaffolds for tendon tissue engineering, ACS BIOMATERIALS SCIENCE & ENGINEERING, ISSN: 2373-9878, DOI: 10.1021/acsbiomaterials.8b01647, Vol.5, No.6, pp.2953-2964, 2019

Abstract:
Tendon injuries are frequent and occur in the elderly, young, and athletic populations. The inadequate number of donors combined with many challenges associated with autografts, allografts, xenografts, and prosthetic devices have added to the value of engineering biological substitutes, which can be implanted to repair the damaged tendons. Electrospun scaffolds have the potential to mimic the native tissue structure along with desired mechanical properties and, thus, have attracted noticeable attention. In order to improve the biological responses of these fibrous structures, we designed and fabricated 3D multilayered composite scaffolds, where an electrospun nanofibrous substrate was coated with a thin layer of cell-laden hydrogel. The whole construct composition was optimized to achieve adequate mechanical and physical properties as well as cell viability and proliferation. Mesenchymal stem cells (MSCs) were differentiated by the addition of bone morphogenetic protein 12 (BMP-12). To mimic the natural function of tendons, the cell-laden scaffolds were mechanically stimulated using a custom-built bioreactor. The synergistic effect of mechanical and biochemical stimulation was observed in terms of enhanced cell viability, proliferation, alignment, and tenogenic differentiation. The results suggested that the proposed constructs can be used for engineering functional tendons.

Keywords:
tendon tissue engineering, composite scaffolds, nanofibrous materials, mechanical stimulation, stem cell differentiation

Affiliations:
Rinoldi C. - other affiliation
Fallahi A. - Paul Scherrer Institut (CH)
Yazdi I.K. - Massachusetts Institute of Technology (US)
Paras J.C. - Massachusetts Institute of Technology (US)
Kijeńska-Gawrońska E. - Warsaw University of Technology (PL)
Trujillo-de Santiago G. - Massachusetts Institute of Technology (US)
Tuoheti A. - Politecnico di Torino (IT)
Demarchi D. - Politecnico di Torino (IT)
Annabi N. - Massachusetts Institute of Technology (US)
Khademhosseini A. - Massachusetts Institute of Technology (US)
Święszkowski W. - other affiliation
Tamayol A. - Massachusetts Institute of Technology (US)
19.  Rinoldi C., Costantini M., Kijeńska-Gawrońska E., Testa S., Fornetti E., Heljak M., Ćwiklińska M., Buda R., Baldi J., Cannata S., Guzowski J., Gargioli C., Khademhosseini A., Święszkowski W., Tendon tissue engineering: effects of mechanical and biochemical stimulation on stem cell alignment on cell‐laden hydrogel yarns, ADVANCED HEALTHCARE MATERIALS, ISSN: 2192-2659, DOI: 10.1002/adhm.201801218, Vol.8, No.7, pp.1801218-1-10, 2019

Abstract:
Fiber-based approaches hold great promise for tendon tissue engineering enabling the possibility of manufacturing aligned hydrogel filaments that can guide collagen fiber orientation, thereby providing a biomimetic micro-environment for cell attachment, orientation, migration, and proliferation. In this study, a 3D system composed of cell-laden, highly aligned hydrogel yarns is designed and obtained via wet spinning in order to reproduce the morphology and structure of tendon fascicles. A bioink composed of alginate and gelatin methacryloyl (GelMA) is optimized for spinning and loaded with human bone morrow mesenchymal stem cells (hBM-MSCs). The produced scaffolds are subjected to mechanical stretching to recapitulate the strains occurring in native tendon tissue. Stem cell differentiation is promoted by addition of bone morphogenetic protein 12 (BMP-12) in the culture medium. The aligned orientation of the fibers combined with mechanical stimulation results in highly preferential longitudinal cell orientation and demonstrates enhanced collagen type I and III expression. Additionally, the combination of biochemical and mechanical stimulations promotes the expression of specific tenogenic markers, signatures of efficient cell differentiation towards tendon. The obtained results suggest that the proposed 3D cell-laden aligned system can be used for engineering of scaffolds for tendon regeneration.

Keywords:
hydrogel fibers, static mechanical stretching, stem cell alignment, tenogenic differentiation, wet spinning

Affiliations:
Rinoldi C. - other affiliation
Costantini M. - Sapienza University of Rome (IT)
Kijeńska-Gawrońska E. - Warsaw University of Technology (PL)
Testa S. - Tor Vergata Rome University (IT)
Fornetti E. - Tor Vergata Rome University (IT)
Heljak M. - Warsaw University of Technology (PL)
Ćwiklińska M. - Institute of Physical Chemistry, Polish Academy of Sciences (PL)
Buda R. - Institute of Physical Chemistry, Polish Academy of Sciences (PL)
Baldi J. - Tor Vergata Rome University (IT)
Cannata S. - Tor Vergata Rome University (IT)
Guzowski J. - Institute of Physical Chemistry, Polish Academy of Sciences (PL)
Gargioli C. - Tor Vergata Rome University (IT)
Khademhosseini A. - Massachusetts Institute of Technology (US)
Święszkowski W. - other affiliation
20.  Sardelli L., Pacheco D.P., Zorzetto L., Rinoldi C., Święszkowski W., Petrini P., Engineering biological gradients, Journal of Applied Biomaterials & Functional Materials, ISSN: 2280-8000, DOI: 10.1177/2280800019829023, Vol.17, No.1, pp.2280800019829023-1-15, 2019

Abstract:
Biological gradients profoundly influence many cellular activities, such as adhesion, migration, and differentiation, which are the key to biological processes, such as inflammation, remodeling, and tissue regeneration. Thus, engineered structures containing bioinspired gradients can not only support a better understanding of these phenomena, but also guide and improve the current limits of regenerative medicine. In this review, we outline the challenges behind the engineering of devices containing chemical-physical and biomolecular gradients, classifying them according to gradient-making methods and the finalities of the systems. Different manufacturing processes can generate gradients in either in-vitro systems or scaffolds, which are suitable tools for the study of cellular behavior and for regenerative medicine; within these, rapid prototyping techniques may have a huge impact on the controlled production of gradients. The parallel need to develop characterization techniques is addressed, underlining advantages and weaknesses in the analysis of both chemical and physical gradients.

Keywords:
graded scaffolds, rapid prototyping, bioinspired, microfluidic, gradient characterization, cartilage, bone

Affiliations:
Sardelli L. - Politecnico di Milano (IT)
Pacheco D.P. - Politecnico di Milano (IT)
Zorzetto L. - University of Liège (BE)
Rinoldi C. - other affiliation
Święszkowski W. - other affiliation
Petrini P. - Politecnico di Milano (IT)
21.  Saghazadeh S., Rinoldi C., Schot M., Kashaf S.S., Sharifi F., Jalilian E., Nuutila K., Giatsidis G., Mostafalu P., Derakhshandeh H., Yue K., Święszkowski W., Memic A., Tamayol A., Khademhosseini A., Drug delivery systems and materials for wound healing applications, Advanced Drug Delivery Reviews, ISSN: 0169-409X, DOI: 10.1016/j.addr.2018.04.008, Vol.127, pp.138-166, 2018

Abstract:
Chronic, non-healing wounds place a significant burden on patients and healthcare systems, resulting in impaired mobility, limb amputation, or even death. Chronic wounds result from a disruption in the highly orchestrated cascade of events involved in wound closure. Significant advances in our understanding of the pathophysiology of chronic wounds have resulted in the development of drugs designed to target different aspects of the impaired processes. However, the hostility of the wound environment rich in degradative enzymes and its elevated pH, combined with differences in the time scales of different physiological processes involved in tissue regeneration require the use of effective drug delivery systems. In this review, we will first discuss the pathophysiology of chronic wounds and then the materials used for engineering drug delivery systems. Different passive and active drug delivery systems used in wound care will be reviewed. In addition, the architecture of the delivery platform and its ability to modulate drug delivery are discussed. Emerging technologies and the opportunities for engineering more effective wound care devices are also highlighted.

Keywords:
Wound healing, Drug delivery, Transdermal delivery, Microtechnologies, Nanotechnologies

Affiliations:
Saghazadeh S. - Massachusetts Institute of Technology (US)
Rinoldi C. - other affiliation
Schot M. - Massachusetts Institute of Technology (US)
Kashaf S.S. - Massachusetts Institute of Technology (US)
Sharifi F. - Massachusetts Institute of Technology (US)
Jalilian E. - Massachusetts Institute of Technology (US)
Nuutila K. - Brigham and Women's Hospital (US)
Giatsidis G. - Brigham and Women's Hospital (US)
Mostafalu P. - Massachusetts Institute of Technology (US)
Derakhshandeh H. - University of Nebraska (US)
Yue K. - Massachusetts Institute of Technology (US)
Święszkowski W. - other affiliation
Memic A. - King Abdulaziz University (SA)
Tamayol A. - Massachusetts Institute of Technology (US)
Khademhosseini A. - Massachusetts Institute of Technology (US)
22.  Chlanda A., Kijeńska E., Rinoldi C., Tarnowski M., Wierzchoń T., Święszkowski W., Structure and physico-mechanical properties of low temperature plasma treated electrospun nanofibrous scaffolds examined with atomic force microscopy, Micron, ISSN: 0968-4328, DOI: 10.1016/j.micron.2018.01.012, Vol.107, pp.79-84, 2018

Abstract:
Electrospun nanofibrous scaffolds are willingly used in tissue engineering applications due to their tunable mechanical, chemical and physical properties. Additionally, their complex openworked architecture is similar to the native extracellular matrix of living tissue. After implantation such scaffolds should provide sufficient mechanical support for cells. Moreover, it is of crucial importance to ensure sterility and hydrophilicity of the scaffold. For this purpose, a low temperature surface plasma treatment can be applied. In this paper, we report physico-mechanical evaluation of stiffness and adhesive properties of electrospun mats after their exposition to low temperature plasma. Complex morphological and mechanical studies performed with an atomic force microscope were followed by scanning electron microscope imaging and a wettability assessment. The results suggest that plasma treatment can be a useful method for the modification of the surface of polymeric scaffolds in a desirable manner. Plasma treatment improves wettability of the polymeric mats without changing their morphology.

Keywords:
Atomic force microscopy, Surface modification, Electrospun fibers, RF plasma treatment, Tissue engineering, Nanomaterial

Affiliations:
Chlanda A. - Warsaw University of Technology (PL)
Kijeńska E. - Warsaw University of Technology (PL)
Rinoldi C. - other affiliation
Tarnowski M. - Warsaw University of Technology (PL)
Wierzchoń T. - Warsaw University of Technology (PL)
Święszkowski W. - other affiliation
23.  Rinoldi C., Kijeńska E., Chlanda A., Choińska E., Khenoussi N., Tamayol A., Khademhosseini A., Święszkowski W., Nanobead-on-string composites for tendon tissue engineering, JOURNAL OF MATERIALS CHEMISTRY B , ISSN: 2050-7518, DOI: 10.1039/c8tb00246k, Vol.6, No.19, pp.3116-3127, 2018

Abstract:
Tissue engineering holds great potential in the production of functional substitutes to restore, maintain or improve the functionality in defective or lost tissues. So far, a great variety of techniques and approaches for fabrication of scaffolds have been developed and evaluated, allowing researchers to tailor precisely the morphological, chemical and mechanical features of the final constructs. Electrospinning of biocompatible and biodegradable polymers is a popular method for producing homogeneous nanofibrous structures, which might reproduce the nanosized organization of the tendons. Moreover, composite scaffolds obtained by incorporating nanoparticles within electrospun fibers have been lately explored in order to enhance the properties and the functionalities of the pristine polymeric constructs. The present study is focused on the design and fabrication of biocompatible electrospun nanocomposite fibrous scaffolds for tendon regeneration. A mixture of poly(amide 6) and poly(caprolactone) is electrospun to generate constructs with mechanical properties comparable to that of native tendons. To improve the biological activity of the constructs and modify their topography, wettability, stiffness and degradation rate, we incorporated silica particles into the electrospun substrates. The use of nanosize silica particles enables us to form bead-on-fiber topography, allowing the better exposure of ceramic particles to better profit their beneficial characteristics. In vitro biocompatibility studies using L929 fibroblasts demonstrated that the presence of 20 wt% of silica nanoparticles in the engineered scaffolds enhanced cell spreading and proliferation as well as extracellular matrix deposition. The results reveal that the electrospun nanocomposite scaffold represents an interesting candidate for tendon tissue engineering.

Affiliations:
Rinoldi C. - other affiliation
Kijeńska E. - Warsaw University of Technology (PL)
Chlanda A. - Warsaw University of Technology (PL)
Choińska E. - Warsaw University of Technology (PL)
Khenoussi N. - Université de Haute Alsace (FR)
Tamayol A. - Massachusetts Institute of Technology (US)
Khademhosseini A. - Massachusetts Institute of Technology (US)
Święszkowski W. - other affiliation
24.  Nasajpour A., Ansari S., Rinoldi C., Rad A.S., Aghaloo T., Shin S.R., Mishra Y.K., Adelung R., Święszkowski W., Annabi N., Khademhosseini A., Moshaverinia A., Tamayol A., A Multifunctional Polymeric Periodontal Membrane with Osteogenic and Antibacterial Characteristics, Advanced Functional Materials, ISSN: 1616-301X, DOI: 10.1002/adfm.201703437, Vol.28, No.3, pp.1703437-1-8, 2017

Abstract:
Periodontitis is a prevalent chronic, destructive inflammatory disease affecting tooth‐supporting tissues in humans. Guided tissue regeneration strategies are widely utilized for periodontal tissue regeneration generally by using a periodontal membrane. The main role of these membranes is to establish a mechanical barrier that prevents the apical migration of the gingival epithelium and hence allowing the growth of periodontal ligament and bone tissue to selectively repopulate the root surface. Currently available membranes have limited bioactivity and regeneration potential. To address such challenges, an osteoconductive, antibacterial, and flexible poly(caprolactone) (PCL) composite membrane containing zinc oxide (ZnO) nanoparticles is developed. The membranes are fabricated through electrospinning of PCL and ZnO particles. The physical properties, mechanical characteristics, and in vitro degradation of the engineered membrane are studied in detail. Also, the osteoconductivity and antibacterial properties of the developed membrane are analyzed in vitro. Moreover, the functionality of the membrane is evaluated with a rat periodontal defect model. The results confirmed that the engineered membrane exerts both osteoconductive and antibacterial properties, demonstrating its great potential for periodontal tissue engineering.

Keywords:
electrospinning, guided tissue regeneration, osteoconductive, periodontal regeneration, zinc oxide

Affiliations:
Nasajpour A. - Massachusetts Institute of Technology (US)
Ansari S. - University of California (US)
Rinoldi C. - other affiliation
Rad A.S. - Massachusetts Institute of Technology (US)
Aghaloo T. - University of California (US)
Shin S.R. - Massachusetts Institute of Technology (US)
Mishra Y.K. - Kiel University (DE)
Adelung R. - Kiel University (DE)
Święszkowski W. - other affiliation
Annabi N. - Massachusetts Institute of Technology (US)
Khademhosseini A. - Massachusetts Institute of Technology (US)
Moshaverinia A. - University of California (US)
Tamayol A. - Massachusetts Institute of Technology (US)
25.  Celikkin N., Rinoldi C., Costantini M., Trombetta M., Rainer A., Święszkowski W., Naturally derived proteins and glycosaminoglycan scaffolds for tissue engineering applications, Materials Science and Engineering C, ISSN: 0928-4931, DOI: 10.1016/j.msec.2017.04.016, Vol.78, pp.1277-1299, 2017

Abstract:
Tissue engineering (TE) aims to mimic the complex environment where organogenesis takes place using advanced materials to recapitulate the tissue niche. Cells, three-dimensional scaffolds and signaling factors are the three main and essential components of TE. Over the years, materials and processes have become more and more sophisticated, allowing researchers to precisely tailor the final chemical, mechanical, structural and biological features of the designed scaffolds. In this review, we will pose the attention on two specific classes of naturally derived polymers: fibrous proteins and glycosaminoglycans (GAGs). These materials hold great promise for advances in the field of regenerative medicine as i) they generally undergo a fast remodeling in vivo favoring neovascularization and functional cells organization and ii) they elicit a negligible immune reaction preventing severe inflammatory response, both representing critical requirements for a successful integration of engineered scaffolds with the host tissue. We will discuss the recent achievements attained in the field of regenerative medicine by using proteins and GAGs, their merits and disadvantages and the ongoing challenges to move the current concepts to practical clinical application.

Keywords:
Natural polymers, Hydrogel scaffolds, Glycosaminoglycans (GAGs), Fibrous proteins, Regenerative medicine

Affiliations:
Celikkin N. - Warsaw University of Technology (PL)
Rinoldi C. - other affiliation
Costantini M. - Sapienza University of Rome (IT)
Trombetta M. - Università Campus Bio-Medico di Roma (IT)
Rainer A. - Università Campus Bio-Medico di Roma (IT)
Święszkowski W. - other affiliation

List of chapters in recent monographs
1. 
Święszkowski W., Paradiso A., Volpi M., Rinoldi C., Idaszek J., Costantini M., Biofabrication: an integrated bioengineering approach for the automated fabrication of biological structures for clinical and research applications, rozdział: Mimicking nature with biofabrication, Pàtron, pp.31-50, 2021
2. 
Costantini M., Testa S., Rinoldi C., Celikkin N., Idaszek J., Colosi C., Gargioli C., Święszkowski W., Barbetta A., Biomaterials Science Series, Biofabrication and 3D Tissue Modeling, rozdział: 3D Tissue Modelling of Skeletal Muscle Tissue, Royal Society of Chemistry, Edited by Dong-Woo Cho, 3, pp.184-215, 2019

Conference papers
1.  Zargarian S.S., Rinoldi C., Ziai Y., Nakielski P., and Pierini F., DEVELOPMENT OF CONDUCTIVE STIMULI-RESPONSIVE FIBROUS HYDROGELS FOR NEURAL INTERFACES, TERMIS-EU 2022, Tissue Engineering and Regenerative Medicine International Society European Chapter Conference 2022, 2022-06-28/07-01, Kraków (PL), No.2022, pp.2, 2022

Conference abstracts
1.  Zargarian S.S., Rinoldi C., Ziai Y., Nakielski P., Pierini F., Synthesis and Fabrication of Thermoresponsive Cross-linkable Poly(N-Isopropylacrylamide-Co-Glycidyl Methacrylate), Chemeet, International Chemistry Conference, 2022-06-27/06-29, Madrid, Spain. Hybrid Conference (ES), No.2022, pp.3-4, 2022

Abstract:
Due to their importance in various fields of bio-nanotechnology, the synthesis of thermoresponsive smart polymers has been the focus of recent research. Poly(N-isopropylacrylamide) (PNIPAAm) is a well-known thermal-stimulus responsive polymer that has attracted much attention. For PNIPAAm hydrogels to acquire fast thermo-responsive properties, water molecules must have quick access to the entire material. However, isotropic PNIPAAm-based hydrogels have a slow stimulus-responsivity. Hydrophilic cross-linkable nanostructures are gaining interest as a viable alternative to traditional hydrogels to address this issue. System miniaturization via electrospinning exhibits nanostructures with significantly larger porosity and specific surface area. If the constituting hydrophilic polymer of the electrospun fibrous material were cross-linkable, the resulting would display a rapid hydration/dehydration response. As a result, developing a new class of cross-linkable PNIPAAm copolymers is highly desired.

Affiliations:
Zargarian S.S. - IPPT PAN
Rinoldi C. - IPPT PAN
Ziai Y. - IPPT PAN
Nakielski P. - IPPT PAN
Pierini F. - IPPT PAN
2.  Ziai Y., Rinoldi C., Nakielski P., Kowalewski T.A., Pierini F., Chameleon-inspired multifunctional plasmonic nanoplatforms for biosensing applications, TERMIS EU 2022, 2022-06-28/07-01, Krakow (PL), No.PS16.11, pp.1, 2022
3.  Zargarian S.S., Rinoldi C., Ziai Y., Nakielski P., and Pierini F., Development of Conductive Fibrous Hydrogels for Neural Interfaces, 4th INTERDISCIPLINARY FNP CONFERENCE, 2022-10-06/10-07, Warsaw (PL), No.2022, pp.8, 2022
4.  Rinoldi C., Ziai Y., Zembrzycki K., Pierini F., CONDUCTIVE HYDROGEL NANOCOMPOSITE-BASED NEURAL INTERFACE FOR IN VIVO RECORDING OF BRAIN CORTEX SIGNALS, TERMIS-EU 2022, Tissue Engineering and Regenerative Medicine International Society European Chapter Conference 2022, 2022-06-28/07-01, Kraków (PL), No.262, pp.1, 2022
5.  Nakielski P., Rinoldi C., Pruchniewski M., Rybak D., Jezierska-Woźniak K., Gazińska M., Strojny B., Grodzik M., Maksymowicz W., Pierini F., Injectable nanofibrous microscaffolds, EHDAES, European Symposium on Electrohydrodynamic Atomization and Electrospinning, 2022-04-27/04-29, Napoli (IT), pp.1, 2022
6.  Nakielski P., Rinoldi C., Pruchniewski M., Rybak D., Jezierska-Woźniak K., Gazińska M., Strojny B., Grodzik M., Maksymowicz W., Pierini F., Injectable nanofibrous microscaffolds for cell and drug delivery, TERMIS-EU 2022, Tissue Engineering and Regenerative Medicine International Society European Chapter Conference 2022, 2022-06-28/07-01, Kraków (PL), pp.1, 2022
7.  Nakielski P., Rinoldi C., Pruchniewski M., Rybak D., Urbanek O., Jezierska- Woźniak K., Grodzik M., Maksymowicz W., Pierini F., Injectable microscaffolds for IVD regeneration, 2022 eCM20: Cartilage and Disc Repair and Regeneration, 2022-06-15/06-18, Davos (CH), pp.33-33, 2022
8.  Haghighat Bayan M., Rinoldi C., Nakielski P., Pierini F., Stimuli-responsive face mask-based on electrospun nanofibers, ESB 2022, 32nd Annual Conference of the European Society for Biomaterials, 2022-09-04/09-08, Bordeaux (FR), pp.195, 2022
9.  Ziai Y., Rinoldi C., Pawłowska S., Nakielski P., Kowalewski T.A., Pierini F., DESIGN AND CHARACTERIZATION OF PHOTORESPONSIVE MULTIFUNCTIONAL HYDROGEL-BASED COMPOSITE PLATFORM, TERMIS 6th World Congress, Tissue Engineering and Regenerative Medicine International Society 6th World Congress 2021, 2021-11-15/11-19, Maastricht (NL), No.506, pp.430, 2021
10.  Rinoldi C., Pawłowska S., Nakielski P., Ziai Y., Urbanek O., Kowalewski T.A., Pierini F., LIGHT-ASSISTED ELECTROSPINNING OF CORE-SHELL P(NIPAAM-CO-NIPMAAM) HYDROGEL-BASED NANOFIBERS FOR THERMALLY SELF-REGULATED DRUG DELIVERY, TERMIS 6th World Congress, Tissue Engineering and Regenerative Medicine International Society 6th World Congress 2021, 2021-11-15/11-19, Maastricht (NL), No.286, pp.246, 2021
11.  Rinoldi C., Lanzi M., Fiorelli R., Nakielski P., Zembrzycki K., Kowalewski T.A., Urbanek O., Grippo V., Jezierska-Woźniak K., Maksymowicz W., Camposeo A., Bilewicz R., Pisignano D., Sanai N., Pierini F., Conductive interpenetrating polymer network hydrogel for neural tissue engineering and 3D printing applications, ESB 2021, 31st Annual Conference of the European Society for Biomaterials, 2021-09-05/09-09, Porto (PT), No.PS02-07-224, pp.1691-1692, 2021
12.  Zargarian S., Rinoldi C., Ziai Y., Nakielski P., Pierini F., Fabrication of poly (N-isopropylacrylamide-co-glycidyl methacrylate) electrospun hydrogel fibers, NanoInnovation 2021 Conference, 2021-09-21/09-24, Rome (IT), pp.89, 2021
13.  Rinoldi C., Pawłowska S., Nakielski P., Ziai Y., Urbanek O., Kowalewski T.A., Pierini F., Electrospinning of core-shell cross-linked P(NIPAAm-co-NIPMAAm) for tissue engineering, WBC2020, 11th World Biomaterials Congress, 2020-12-11/12-15, online (GB), No.4190, pp.1, 2020
14.  Pierini F., Nakielski P., Pawłowska S., Rinoldi C., Ziai Y., Urbanek-Świderska O., De Sio L., Calogero A., Lanzi M., Zembrzycki K., Pruchniewski M., Salatelli E., Kowalewski T.A., Yarin A., Nature-inspired smart drug delivery platforms based on electrospun nanofibers and plasmonic hydrogels for near-infrared light-controlled polytherapy, Polymer Connect, Polymer Science and Composite Materials Conference, 2020-02-26/02-28, LISBON (PT), pp.7, 2020

Patents
Filing No./Date
Filing Publication
Autors
Title
Protection Area, Applicant Name
Patent Number
Date of Grant
pdf
435749
2020-10-21
BUP 17/2022
2022-04-25
Pierini F., Nakielski P., Rinoldi C., Pawłowska S., Ding B., Li X., Si Y.
On-demand drug delivery nanoplatform, method of its production and application
PL, Instytut Podstawowych Problemów Techniki PAN
242911
WUP 20/2023
2023-05-15



Category A Plus

IPPT PAN

logo ippt            Pawińskiego 5B, 02-106 Warsaw
  +48 22 826 12 81 (central)
  +48 22 826 98 15
 

Find Us

mapka
© Institute of Fundamental Technological Research Polish Academy of Sciences 2023