Institute of Fundamental Technological Research
Polish Academy of Sciences


Olga Cegielska, MSc

Laboratory of Polymers and Biomaterials (SPPiB)
position: Specialist
PhD student
telephone: (+48) 22 826 12 81 ext.: 211
room: 343
ORCID: 0000-0002-3074-6474

Recent publications
1.  Altangerel A., Moazzami Goudarzi Z., Cegielska O., Gradys A.D., Kołbuk-Konieczny D., Kalaska B., Ruszczyńska A., Sajkiewicz P.Ł., A facile one-stone-two-birds strategy for fabricating multifunctional 3D nanofibrous scaffolds, Biomaterials Science, ISSN: 2047-4849, DOI: 10.1039/D3BM00837A, Vol.11, No.16, pp.5502-5516, 2023

Local bacterial infections lead to delayed wound healing and in extreme cases, such as diabetic foot ulcers, to non-healing due to the impaired cellular function in such wounds. Thus, many scientists have focused on developing advanced therapeutic platforms to treat infections and promote cellular proliferation and angiogenesis. This study presents a facile approach for designing nanofibrous scaffolds in three dimensions (3D) with enhanced antibacterial activity to meet the need of treating chronic diabetic wounds. Being a cationic surfactant as well as an antimicrobial agent, octenidine (OCT) makes a 2D membrane hydrophilic, enabling it to be modified into a 3D scaffold in a “one stone, two birds” manner. Aqueous sodium borohydride (NaBH4) solution plays a dual role in the fabrication process, functioning as both a reducing agent for the in situ synthesis of silver nanoparticles (Ag NPs) anchored on the nanofiber surface and a hydrogen gas producer for expanding the 2D membranes into fully formed 3D nanofiber scaffolds, as demonstrated by morphological analyses. Various techniques were used to characterize the developed scaffold (e.g., SEM, XRD, DSC, FTIR, and surface wettability), demonstrating a multilayered porous structure and superhydrophilic properties besides showing sustained and prolonged release of OCT (61% ± 1.97 in 144 h). Thanks to the synergistic effect of OCT and Ag NPs, the antibacterial performance of the 3D scaffold was significantly higher than that of the 2D membrane. Moreover, cell viability was studied in vitro on mouse fibroblasts L929, and the noncytotoxic character of the 3D scaffold was confirmed. Overall, it is shown that the obtained multifunctional 3D scaffold is an excellent candidate for diabetic wound healing and skin repair.

Altangerel A. - IPPT PAN
Moazzami Goudarzi Z. - IPPT PAN
Cegielska O. - IPPT PAN
Gradys A.D. - IPPT PAN
Kołbuk-Konieczny D. - IPPT PAN
Kalaska B. - other affiliation
Ruszczyńska A. - other affiliation
Sajkiewicz P.Ł. - IPPT PAN
2.  Cegielska O., Sierakowski M., Sajkiewicz P., Lorenz K., Kogermann K., Mucoadhesive brinzolamide-loaded nanofibers for alternative glaucoma treatment, European Journal of Pharmaceutics and Biopharmaceutics, ISSN: 0939-6411, DOI: 10.1016/j.ejpb.2022.09.008, Vol.180, pp.48-62, 2022

Despite the advances in the field of pharmaceutical materials and technology, topical administration remains a method of choice for the treatment of eye diseases such as glaucoma, with eye drops being a leading dosage form. Their main disadvantage is a very short drug residence time and thus poor drug bioavailability, leading to the necessity of continuous repeated dosing. Mucoadhesive electrospun nanofibers are promising candidates for overcoming these challenges, while still benefiting from topical ocular administration. As an alternative for eye drops, a nanofibrous drug delivery system (DDS) for the delivery of brinzolamide (BRZ), based on β-cyclodextrin (β-CD), hydroxypropyl cellulose (HPC) and polycaprolactone (PCL), was designed. The results showed β-CD/BRZ guest–host interactions, successful drug incorporation into the nanofibers, and the possibility of more accurate dosing in comparison with the control eye drops. Drug permeation through sheep corneas was almost linear in time, achieving therapeutic concentrations in the receptor medium, and mucoadhesion to sheep eye mucosa was relatively high in case of formulations with high HPC content. All formulations were biocompatible, their mechanical properties were sufficient to handle them without caution and UV irradiation was suitable to reduce bioburden of the fibers matrix, yet no antibacterial properties of BRZ were observed.

Drug delivery system, Nanofibers, Electrospinning, Cyclodextrins, Glaucoma, Mucoadhesion, Topical administration

Cegielska O. - IPPT PAN
Sierakowski M. - other affiliation
Sajkiewicz P. - IPPT PAN
Lorenz K. - other affiliation
Kogermann K. - other affiliation
3.  Cegielska O., Sajkiewicz P., Targeted drug delivery systems for the treatment of glaucoma: most advanced systems review, Polymers, ISSN: 2073-4360, DOI: 10.3390/polym11111742, Vol.11, No.11, pp.1742-1-18, 2019

Each year, new glaucoma drug delivery systems are developed. Due to the chronic nature of the disease, it requires the inconvenient daily administration of medications. As a result of their elution from the eye surface and penetration to the bloodstream through undesired permeation routes, the bioavailability of active compounds is low, and systemic side effects occur. Despite numerous publications on glaucoma drug carriers of controlled drug release kinetics, only part of them consider drug permeation routes and, thus, carriers' location, as an important factor affecting drug delivery. In this paper, we try to demonstrate the importance of the delivery proximal to glaucoma drug targets. The targeted delivery can significantly improve drug bioavailability, reduce side effects, and increase patients' compliance compared to both commercial and scientifically developed formulations that can spread over the eye surface or stay in contact with conjunctival sac. We present a selection of glaucoma drug carriers intended to be placed on cornea or injected into the aqueous humor and that have been made by advanced materials using hi-tech forming methods, allowing for effective and convenient sustained antiglaucoma drug delivery.

hydrogels, nanofibers, electrospinning, glaucoma, ophthalmology

Cegielska O. - IPPT PAN
Sajkiewicz P. - IPPT PAN

Conference abstracts
1.  Cegielska O., Sajkiewicz P., Sierakowski M., Electrospun brinzolamide carrier – potential antiglaucoma drug delivery platform, ESB 2021, 31st Annual Conference of the European Society for Biomaterials, 2021-09-05/09-09, Porto (PT), pp.1338-1339, 2021
2.  Cegielska O., Sajkiewicz P., Mucoadhesive nanofibers for brinzolamide delivery, TERMIS EU 2019, TERMIS European Chapter Meeting 2019, Tissue Engineering Therapies: From Concept to Clinical Translation & Commercialisation, 2019-05-27/05-31, Rodos (GR), pp.1064, 2019

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