Oliwia Jeznach, M.Sc., Eng.

Laboratory of Polymers and Biomaterials (SPPiB)
position: doctoral student
telephone: (+48) 22 826 12 81 ext.: 425
room: 338
e-mail: ojeznach

Recent publications
1.Kołbuk D., Jeznach O., Wrzecionek M., Gadomska-Gajadhur A., Poly(glycerol succinate) as an eco-friendly component of PLLA and PLCL fibres towards medical applications, Polymers, ISSN: 2073-4360, DOI: 10.3390/polym12081731, Vol.12, No.8, pp.1731-1-17, 2020
Abstract:

This study was conducted as a first step in obtaining eco-friendly fibres for medical applications using a synthesised oligomer poly(glycerol succinate) (PGSu) as an additive for synthetic poly(L-lactic acid) (PLLA) and poly (L-lactide-co-caprolactone) (PLCL). The effects of the oligomer on the structure formation, morphology, crystallisation behaviour, and mechanical properties of electrospun bicomponent fibres were investigated. Nonwovens were investigated by means of scanning electron microscopy (SEM), wide angle X-ray scattering (WAXS), differential scanning calorimetry (DSC), and mechanical testing. The molecular structure of PLLA fibres is influenced by the presence of PGSu mainly acting as an enhancer of molecular orientation. In the case of semicrystalline PLCL, chain mobility was enhanced by the presence of PGSu molecules, and the crystallinity of bicomponent fibres increased in relation to that of pure PLCL. The mechanical properties of bicomponent fibres were influenced by the level of PGSu present and the extent of crystal formation of the main component. An in vitro study conducted using L929 cells confirmed the biocompatible character of all bicomponent fibres.

Keywords:

poly(glycerol succinate), plasticiser, eco-friendly polymer, electrospinning, hyperbranched polyester

Affiliations:
Kołbuk D.-IPPT PAN
Jeznach O.-IPPT PAN
Wrzecionek M.-other affiliation
Gadomska-Gajadhur A.-Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)
2.Jeznach O., Kołbuk D., Sajkiewicz P., Aminolysis of various aliphatic polyesters in a form of nanofibers and films, Polymers, ISSN: 2073-4360, DOI: 10.3390/polym11101669, Vol.11, No.10, pp.1669-1-16, 2019
Abstract:

Surface functionalization of polymer scaffolds is a method used to improve interactions of materials with cells. A frequently used method for polyesters is aminolysis reaction, which introduces free amine groups on the surface. In this study, nanofibrous scaffolds and films of three different polyesters–polycaprolactone (PCL), poly(lactide-co-caprolactone) (PLCL), and poly(l-lactide) (PLLA) were subjected to this type of surface modification under the same conditions. Efficiency of aminolysis was evaluated on the basis of ninhydrin tests and ATR–FTIR spectroscopy. Also, impact of this treatment on the mechanical properties, crystallinity, and wettability of polyesters was compared and discussed from the perspective of aminolysis efficiency. It was shown that aminolysis is less efficient in the case of nanofibers, particularly for PCL nanofibers. Our hypothesis based on the fundamentals of classical high speed spinning process is that the lower efficiency of aminolysis in the case of nanofibers is associated with the radial distribution of crystallinity of electrospun fiber with more crystalline skin, strongly inhibiting the reaction. Moreover, the water contact angle results demonstrate that the effect of free amino groups on wettability is very different depending on the type and the form of polymer. The results of this study can help to understand fundamentals of aminolysis-based surface modification.

Keywords:

aminolysis, polyester, electrospinning, nanofibers, film, surface chemical modification

Affiliations:
Jeznach O.-IPPT PAN
Kołbuk D.-IPPT PAN
Sajkiewicz P.-IPPT PAN
3.Jeznach O., Gajc M., Korzeb K., Kłos A., Orliński K., Stępień R., Krok-Borkowicz M., Rumian Ł., Pietryga K., Reczyńska K., Pamuła E., Pawlak D.A., New calcium-free Na2O-Al2O3-P2O5 bioactive glasses with potential applications in bone tissue engineering, Journal of the American Ceramic Society, ISSN: 0002-7820, DOI: 10.1111/jace.15216, Vol.101, No.2, pp.602-611, 2018
Abstract:

Sodium aluminophosphate glasses were evaluated for their bone repair ability. The glasses belonging to the system 45Na2O–xAl2O3-(55-x)P2O5, with x = (3, 5, 7, 10 mol%) were prepared by a melt-quenching method. We assessed the effect of Al2O3 content on the properties of Na2O–Al2O3–P2O5 (NAP) glasses, which were characterized by density measurements, DSC analyses, solubility, bioactivity in simulated body fluid and cytocompatibility with MG-63 cells. To the best of our knowledge, this is the first investigation of calcium-free Na2O–Al2O3–P2O5 system glasses as bioactive materials for bone tissue engineering.

Keywords:

alumina, bioactive glass, bone tissue engineering, calcium-free bioactive glass, phosphate glass, sodium aluminophosphate glass

Affiliations:
Jeznach O.-other affiliation
Gajc M.-Institute of Electronic Materials Technology (PL)
Korzeb K.-Institute of Electronic Materials Technology (PL)
Kłos A.-Institute of Electronic Materials Technology (PL)
Orliński K.-Institute of Electronic Materials Technology (PL)
Stępień R.-Institute of Electronic Materials Technology (PL)
Krok-Borkowicz M.-AGH University of Science and Technology (PL)
Rumian Ł.-AGH University of Science and Technology (PL)
Pietryga K.-AGH University of Science and Technology (PL)
Reczyńska K.-AGH University of Science and Technology (PL)
Pamuła E.-AGH University of Science and Technology (PL)
Pawlak D.A.-Institute of Electronic Materials Technology (PL)
4.Jeznach O., Kołbuk D., Sajkiewicz P., Injectable hydrogels and nanocomposite hydrogels for cartilage regeneration, Journal of Biomedical Materials Research Part A, ISSN: 1549-3296, DOI: 10.1002/jbm.a.36449, Vol.106, No.10, pp.2762-2776, 2018
Abstract:

Cartilage loss due to age‐related degeneration and mechanical trauma is a significant and challenging problem in the field of surgical medicine. Unfortunately, cartilage tissue can be characterized by the lack of regenerative ability. Limitations of conventional treatment strategies, such as auto‐, allo‐ and xenografts or implants stimulate an increasing interest in the tissue engineering approach to cartilage repair. This review discusses the application of polymer‐based scaffolds, with an emphasis on hydrogels in cartilage tissue engineering. We highlight injectable hydrogels with various micro‐ and nanoparticles, as they constitute a novel and attractive type of scaffolds. We discuss advantages, limitations and future perspectives of injectable nanocomposite hydrogels for cartilage tissue regeneration.

Keywords:

polymers, hydrogels, injectable hydrogels, injectable nanocomposite hydrogels, cartilage repair, cartilage tissue engineering

Affiliations:
Jeznach O.-IPPT PAN
Kołbuk D.-IPPT PAN
Sajkiewicz P.-IPPT PAN
5.Jeznach O., Gajc M., Kłos A., Orliński K., Pawlak D.A., Krok-Borkowicz M., Rumian Ł., Pietryga K., Reczyńska K., Pamuła E., The effect of titanium dioxide addition on physical and biological properties of Na2O-B2O3-P2O5 and CaO-Na2O-P2O5 glasses, ENGINEERING OF BIOMATERIALS / INŻYNIERIA BIOMATERIAŁÓW, ISSN: 1429-7248, Vol.19, No.134, pp.2-7, 2016
Abstract:

Two types of phosphate glasses 50Na2O-20B2O3-30P2O5 (NBP) and 30CaO-20Na2O-50P2O5 (CNP) with different content of TiO2 (0, 3 and 5 mol%) have been prepared by melt-quenching process. TiO2 was added to increase glass network stability. Physical properties of glasses were investigated by density measurements, differential scanning calorimetry and degradation in phosphate buffered saline (PBS). Biological performance of glasses in a direct contact with osteoblast-like MG-63 cells was analysed with the use of resazurin test and live-dead staining. The results show that TiO2 addition increased density, glass transition temperature (Tg) and melting temperature (Tm) of both types of glasses. In the case of NBP glasses presence of TiO2 resulted in their fast degradation in PBS and acidification of cell culture medium. As a consequence such glasses did not support cell adhesion and growth, but they can be considered for e.g. drug delivery systems. On the other hand addition of TiO2 to CNP glasses resulted in enhanced cell adhesion and viability. Particularly positive results were found for CNP glass containing 5% TiO2, so it can be a good candidate as a scaffold material for bone tissue engineering.

Keywords:

phosphate glasses, bioactive glasses, titanium dioxide, tissue engineering

Affiliations:
Jeznach O.-other affiliation
Gajc M.-Institute of Electronic Materials Technology (PL)
Kłos A.-Institute of Electronic Materials Technology (PL)
Orliński K.-Institute of Electronic Materials Technology (PL)
Pawlak D.A.-Institute of Electronic Materials Technology (PL)
Krok-Borkowicz M.-AGH University of Science and Technology (PL)
Rumian Ł.-AGH University of Science and Technology (PL)
Pietryga K.-AGH University of Science and Technology (PL)
Reczyńska K.-AGH University of Science and Technology (PL)
Pamuła E.-AGH University of Science and Technology (PL)

Conference abstracts
1.Sajkiewicz P., Jeznach O., Kołbuk-Konieczny D., Functionalization of Aliphatic Polyester Fibers Formed by Electrospinning - The Effect of Crystallinity on Aminolysis, BIOMATSEN, 4th International Congress on Biomaterials and Biosensors, 2019-05-12/05-18, Fethiye (TR), No.Id-289, pp.96-97, 2019
Keywords:

Nanofibers, polyesters, surface functionalization, aminolysis, structure

Affiliations:
Sajkiewicz P.-IPPT PAN
Jeznach O.-IPPT PAN
Kołbuk-Konieczny D.-IPPT PAN
2.Jeznach O., Kołbuk D., Sajkiewicz P., Immobilization of gelatin on electrospun polyesters nanofibers to enhance biological response, EYEC2019, 8th European Young Engineers Conference, 2019-04-08/04-10, Warszawa (PL), pp.266, 2019
Keywords:

polymers, aminolysis, surface modification, tissue engineering

Affiliations:
Jeznach O.-IPPT PAN
Kołbuk D.-IPPT PAN
Sajkiewicz P.-IPPT PAN
3.Jeznach O., Kołbuk D., Sajkiewicz P., Surface functionalization of polyesters nanofibers via aminolysis and gelatin immobilization, TERMIS EU 2019, TERMIS European Chapter Meeting 2019, Tissue Engineering Therapies: From Concept to Clinical Translation & Commercialisation, 2019-05-27/05-31, Rodos (GR), pp.1606, 2019
4.Kołbuk-Konieczny D., Urbanek-Świderska O., Jeznach O., Hybrid scaffold to anterior cruciate ligament regeneration, TERMIS EU 2019, TERMIS European Chapter Meeting 2019, Tissue Engineering Therapies: From Concept to Clinical Translation & Commercialisation, 2019-05-27/05-31, Rodos (GR), pp.1461, 2019
5.Jeznach O., Wrzecionek M., Ruskowski P., Gadomska-Gajadhur A., Kołbuk D., Effect of poly(glycerol succinate) addition on properties of PLA electrospun fibres, ESB2018, 29th European Conference on Biomaterials, 2018-09-09/09-13, Maastricht (NL), pp.1084-1085, 2018
6.Jeznach O., Surface functionalization of polymer nanofibers for tissue engineering applications, PICETE, Polish-Israeli Conference on Electrospinning and Tissue Engineering, 2018-10-04/10-05, Warszawa (PL), pp.23, 2018

Patents
Filing No./Date
Filing Publication
Autor(s)
Title
Protection Area, Applicant Name
Patent Number
Date of Grant
pdf
426830
2018-08-28
BUP 06/20
2020-03-09
Gadomska-Gajadhur A., Ruśkowski P., Synoradzki L., Wrzecionek M., Kołbuk-Konieczny D., Jeznach O., Budnicka M., Szymaniak M.
Sposób otrzymywania poli(bursztynianiu glicerolu)
PL, Politechnika Warszawska
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