Olga Urbanek-Świderska, M.Sc., Eng.

Laboratory of Polymers and Biomaterials (SPPiB)
position: specialist
telephone: (+48) 22 826 12 81 ext.: 425
room: 338
e-mail: ourbanek

Recent publications
1.Pierini F., Lanzi M., Nakielski P., Pawłowska S., Urbanek O., Zembrzycki K., Kowalewski T.A., Single-Material Organic Solar Cells Based on Electrospun Fullerene-Grafted Polythiophene Nanofibers, Macromolecules, ISSN: 0024-9297, DOI: 10.1021/acs.macromol.7b00857, Vol.50, No.13, pp.4972-4981, 2017
Abstract:

Highly efficient single-material organic solar cells (SMOCs) based on fullerene-grafted polythiophenes were fabricated by incorporating electrospun one-dimensional (1D) nanostructures obtained from polymer chain stretching. Poly(3-alkylthiophene) chains were chemically tailored in order to reduce the side effects of charge recombination which severely affected SMOC photovoltaic performance. This enabled us to synthesize a donor–acceptor conjugated copolymer with high solubility, molecular weight, regioregularity, and fullerene content. We investigated the correlations among the active layer hierarchical structure given by the inclusion of electrospun nanofibers and the solar cell photovoltaic properties. The results indicated that SMOC efficiency can be strongly increased by optimizing the supramolecular and nanoscale structure of the active layer, while achieving the highest reported efficiency value (PCE = 5.58%). The enhanced performance may be attributed to well-packed and properly oriented polymer chains. Overall, our work demonstrates that the active material structure optimization obtained by including electrospun nanofibers plays a pivotal role in the development of efficient SMOCs and suggests an interesting perspective for the improvement of copolymer-based photovoltaic device performance using an alternative pathway.

Affiliations:
Pierini F.-IPPT PAN
Lanzi M.-University of Bologna (IT)
Nakielski P.-IPPT PAN
Pawłowska S.-IPPT PAN
Urbanek O.-IPPT PAN
Zembrzycki K.-IPPT PAN
Kowalewski T.A.-IPPT PAN
2.Urbanek O., Sajkiewicz P., Pierini F., The effect of polarity in the electrospinning process on PCL/chitosan nanofibres' structure, properties and efficiency of surface modification, POLYMER, ISSN: 0032-3861, DOI: 10.1016/j.polymer.2017.07.064, Vol.124, pp.168-175, 2017
Abstract:

The aim of this research was to study the effect of charge polarity applied to the spinning nozzle on the structure and properties of polycaprolactone/chitosan (PCL/CHT) blends, in particular the efficiency of further surface modification by chondroitin sulphate (CS). The observed differences in the morphology and properties of fibres formed at different polarities were interpreted in terms of molecular interactions occurring in the system. FTIR results indicate stronger PCL-chitosan interactions at negative polarity, resulting in lower PCL crystallinity and crystal size distribution determined by DSC, as well as lower wettability. The charge polarity influences PCL/CHT fibre morphology and tailors some of their properties, e.g. wettability, mechanical properties and the efficiency of surface modification. Better efficiency of CS attachment was observed at negative polarity using atomic force microscopy (AFM), and X-ray photoelectron spectroscopy (XPS) is most probably related to higher chitosan content at the fibres' surface being attracted by the negative external potential.

Keywords:

Polycaprolactone/chitosan nanofibres, Charge potential effect in electrospinning, Polycaprolactone-chitosan interactions

Affiliations:
Urbanek O.-IPPT PAN
Sajkiewicz P.-IPPT PAN
Pierini F.-IPPT PAN
3.Urbanek O., Sajkiewicz P., Pierini F., Czerkies M., Kołbuk D., Structure and properties of polycaprolactone/chitosan nonwovens tailored by solvent systems, Biomedical Materials, ISSN: 1748-6041, DOI: 10.1088/1748-605X/aa5647, Vol.12, No.1, pp.015020-1-12, 2017
Abstract:

Electrospinning of chitosan blends is a reasonable idea to prepare fibre mats for biomedical applications. Synthetic and natural components provide, for example, appropriate mechanical strength and biocompatibility, respectively. However, solvent characteristics and the polyelectrolyte nature of chitosan influence the spinnability of these blends. In order to compare the effect of solvent on polycaprolactone/chitosan fibres, two types of the most commonly used solvent systems were chosen, namely 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) and acetic acid (AA)/formic acid (FA). Results obtained by various experimental methods clearly indicated the effect of the solvent system on the structure and properties of electrospun polycaprolactone/chitosan fibres. Viscosity measurements confirmed different polymer–solvent interactions. Various molecular interactions resulting in different macromolecular conformations of chitosan influenced its spinnability and properties. HFIP enabled fibres to be obtained whose average diameter was less than 250 nm while maintaining the brittle and hydrophilic character of the nonwoven, typical for the chitosan component. Spectroscopy studies revealed the formation of chitosan salts in the case of the AA/FA solvent system. Chitosan salts visibly influenced the structure and properties of the prepared fibre mats. The use of AA/FA caused a reduction of Young's modulus and wettability of the proposed blends. It was confirmed that wettability, mechanical properties and the antibacterial effect of polycaprolactone/chitosan fibres may be tailored by selecting an appropriate solvent system. The MTT cell proliferation assay revealed an increase of cytotoxicity to mouse fibroblasts in the case of 25% w/w of chitosan in electrospun nonwovens.

Keywords:

chitosan, electrospinning, PCL/chitosan fibres, solvent system, chitosan salts

Affiliations:
Urbanek O.-IPPT PAN
Sajkiewicz P.-IPPT PAN
Pierini F.-IPPT PAN
Czerkies M.-IPPT PAN
Kołbuk D.-IPPT PAN
4.Mayerberger E.A., Urbanek O., McDaniel R.M., Street R.M., Barsoum M.W., Schauer C.L., Preparation and characterization of polymer-Ti3C2Tx(MXene) composite nanofibers produced via electrospinning, JOURNAL OF APPLIED POLYMER SCIENCE, ISSN: 0021-8995, DOI: 10.1002/app.45295, pp.1-7, 2017
Abstract:

MXene, a recently-discovered family of two-dimensional (2 D) transition metal carbides and/or nitrides, have attracted much interest because of their unique electrical, thermal, and mechanical properties. In this study, poly(acrylic acid) (PAA), polyethylene oxide (PEO), poly(vinyl alcohol) (PVA), and alginate/PEO were electrospun with delaminated Ti3C2 (MXene) flakes. The effect of small additions of delaminated Ti3C2 (1% w/w) on the structure and properties of the nanofibers were investigated and compared with those of the neat polymer nanofibers using scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FT-IR). Ti3C2 had an effect on the solution properties of the polymer and a greater effect on the average fiber diameter. The Ti3C2Tx/PEO solution exhibited the largest change in viscosity and conductivity with an 11% and 73.6% increase over the base polymer, respectively. X-ray diffractograms demonstrated a high degree of crystallization for Ti3C2/PEO and a slight decrease in crystallinity for Ti3C2/PVA.

Keywords:

composite nanofibers, electrospinning, MXene

Affiliations:
Mayerberger E.A.-Drexel University (US)
Urbanek O.-IPPT PAN
McDaniel R.M.-Drexel University (US)
Street R.M.-Drexel University (US)
Barsoum M.W.-Drexel University (US)
Schauer C.L.-Drexel University (US)
5.Urbanek O., Pierini F., Choińska E., Sajkiewicz P., Bil M., Święszkowski W., Effect of hydroxyapatite nanoparticles addition on structure properties of poly(L-lactide-co-glycolide) after gamma sterilization, Polymer Composites, ISSN: 0272-8397, DOI: 10.1002/pc.24028, Vol.1, pp.1-9, 2016
Abstract:

Physical and chemical factors resulting from the sterilization methods may affect the structure and properties of the materials which undergo this procedure. Poly(l-lactide-co-glicolide) (PLGA) is commonly used for medical applications, but, due to its inadequate mechanical properties, it is not recommended for load-bearing applications. One of the methods for improving PLGA mechanical properties is addition of hydroxyapatite nanoparticles (nHAp). The aim of this study was to evaluate the effect of nanoparticles addition on PLGA structure and properties after gamma radiation. According to our results, reduction of the molecular mass caused by gamma radiation was lower for PLGA with nHAp addition. Differential scanning calorimetry (DSC) analysis indicates an increase of crystallinity caused both by nHAp and gamma radiation. The first phenomenon can be explained by heteronucleation, while the second one is most probably related to higher molecular mobility of degrading polymer. Moreover, addition of nanoparticles increases thermal stability and affects the Young's modulus changes after gamma radiation.

Affiliations:
Urbanek O.-IPPT PAN
Pierini F.-IPPT PAN
Choińska E.-Warsaw University of Technology (PL)
Sajkiewicz P.-IPPT PAN
Bil M.-Warsaw University of Technology (PL)
Święszkowski W.-other affiliation

Conference abstracts
1.Urbanek O., Sajkiewicz P., Biomimetically surface modified fibres for cartilage regeneration, TERMIS-EU 2017, European Chapter Meeting of the Tissue Engineering and Regenerative Medicine International Society 2017, 2017-06-26/06-30, Davos (CH), pp.P666, 2017
2.Kołbuk D., Denis P., Urbanek O., Tailoring of mechanical properties by molecular orientation in polymeric scaffolds, BioMaH, BIOMATERIALS FOR HEALTHCARE: Biomaterials for Tissue and Genetic Engineering and the Role of Nanotechnology, 2016-10-17/10-20, Rzym (IT), pp.443-446, 2016
Keywords:

molecular orientation, scaffolds, fibres, tissue engineering

Affiliations:
Kołbuk D.-IPPT PAN
Denis P.-IPPT PAN
Urbanek O.-IPPT PAN
3.Urbanek O., Sajkiewicz P., The effect of polarity on biomimetic surface modification of PCL/chitosan nanofibers formed by electrospinning, ELECTROSPIN 2016, 4th International Conference on Electrospinning, 2016-06-28/07-01, Otranto (IT), pp.1, 2016
4.Urbanek O., Sajkiewicz P., Schauer C., Charge Assisted Tailoring and its Effect on Surface Modification of Chitosan Nanofibers, Fiber Society 2016 Fall Meeting and Technical Conference, 2016-10-10/10-12, Ithaca (US), pp.1, 2016
5.Kowalczyk T., Cwiek K., Urbanek O., Kloskowski T., Pokrywczyńska M., Jundziłł A., Adamowicz J., Zabost E., Noszczyk B., Drewa T., Electrospun micro and nanofibers applied for animal models in urology and wound dressing. Potential applications in cancer treatment, 2nd INTERNATIONAL CONFERENCE ON BIO-BASED POLYMERS AND COMPOSITES, 2014-08-24/08-28, Visegrad (HU), pp.24, 2015
Abstract:

We used the principles of electrospinning to produce materials for applications in regenerative medicine of urinary bladder wall, ureter, wound dressing and potential applications in cancer therapy. Our research is based on biodegradable polymers produced by ring-opening polymerization. Scaffolds of poly(L-lactide-co-caprolactone) (PLCL) gradually degrade leaving no artificial material behind to be replaced by natural extracellular collagen matrix. We formed flat membranes of micro- and nanofibers to carry out regeneration of urinary bladder wall as animal model of cancer treatment. Grafts were tested for biocompatibility and aimed for guided cell growth, yet we were unsuccessful in mechanical compliance of nanomaterial and reconstructed tissue. We tested tubular scaffolds made of nanofibers aimed for ureter tissue engineering. We found stem cells seeding unnecessary. The results of nanomaterial implantation on animal model were better than for collagen matrices. Animal model was also tested for use of nanofibers of human serum albumin as wound dressing. The native structure of the protein was retained to maintain its anti-adhesive properties, despite poor mechanical characteristics. Nanomaterial caused no inflammation and was resorbed during 16 days. Last application of presented materials was targeted drug delivery system made of PLCL nanofibers. Release of anticancer drug complexed with nanoparticles is to be triggered by tumor cells. Such nanomaterial is potential drug delivery system. Acknowledgements: The authors wishes to thank for the cooperation: T. Chmielewski, P. Nakielski, K. Zembrzycki, G. Mikulowski and prof. T. A. Kowalewski from IPPT PAN. The project was partially supported by the National Centre for Research and Development. Grant No. STRATEGMED1/235368/8/NCBR/2014.

Keywords:

electrospinning, nanofibers, regenerative medicine, wound dressing, urology, biodegradable polymers, animal model.

Affiliations:
Kowalczyk T.-IPPT PAN
Cwiek K.-other affiliation
Urbanek O.-IPPT PAN
Kloskowski T.-other affiliation
Pokrywczyńska M.-other affiliation
Jundziłł A.-other affiliation
Adamowicz J.-Nicolaus Copernicus University (PL)
Zabost E.-University of Warsaw (PL)
Noszczyk B.-Medical University of Warsaw (PL)
Drewa T.-Nicolaus Copernicus University (PL)
6.Urbanek O., Pierini F., Kołbuk D., Sajkiewicz P., Effect of positive and negative charge applied during electrospinning on surface chemistry and selected properties of chitosan nanofibers, COST MP1206 Workshop on Electrospinning of Chitosan, 2015-09-02/09-03, Münster (DE), pp.7-8, 2015
Keywords:

electrospinning, fibres, surface, chitosan

Affiliations:
Urbanek O.-IPPT PAN
Pierini F.-IPPT PAN
Kołbuk D.-IPPT PAN
Sajkiewicz P.-IPPT PAN
7.Urbanek o., Kołbuk D., Sajkiewicz P., Cellular Response on Polycaprolactone/Chitosan Crystalinity Tailored by Solvent System in Electrospinning Process, PUZZLE 2015, IV Wrocławska Konferencja Studentów Nauk Technicznych i Ścisłych, 2015-04-18/04-19, Wrocław (PL), pp.22, 2015
Keywords:

chitosan, PCL, cellular responce, electrospinning

Affiliations:
Urbanek o.-IPPT PAN
Kołbuk D.-IPPT PAN
Sajkiewicz P.-IPPT PAN
8.Urbanek O., Kołbuk D., Sajkiewicz P., Odpowiedź komórkowa na krystaliczność włókien polikaprolakton/ chitozan uzyskiwaną z wybranych rozpuszczalników, XIX Krajowa Konferencja Biocybernetyka i Inżynieria Biomedyczna, 2015-10-14/10-16, Warszawa (PL), pp.213, 2015
Keywords:

chitozan, polikaprolakton, electrospinning, L929, cytotoksyczność

Affiliations:
Urbanek O.-IPPT PAN
Kołbuk D.-IPPT PAN
Sajkiewicz P.-IPPT PAN
9.Urbanek O., Bil M., Święszkowski W., The effect of hydroxyapatite nanoparticles addition on crystallinity and surface properties of bioresorbable nanocomposites after gamma sterylization treatment, 4th Summer Symposium on Nanomaterials and Their Application to Biology and Medicine, 2014-06-15/06-18, Poznań (PL), pp.1, 2014
10.Kołbuk D., Denis P., Urbanek O., L929 response on polycaprolactone films with tailored crystallinity, Szkoła Zimowa, 2014-12-15/12-16, Warszawa (PL), pp.29, 2014
Abstract:

Influence of the crystallinity of the substrate on cell proliferation during in-vitro study was highlighted in few articles. Methods of forming 3-D scaffolds usually do not take into account crystallinity optimisation. The aim of proposed presentation is to investigate an influence of polycaprolactone (PCL) crystallinity on cells spreading, their activity and proliferation. PCL Mn 45k and Mn 80k g/mol were used. As a solvents: HFIP (H) and Acetic Acid (AA) were used. Two methods of foil preparation were analysed: -forming from melt (PCL45, PCL80) -forming from 10%wt solution (e.g. PH45, PAA45) Samples were analyzed using interfered-polarization microscopy (MIP) which allows to describe the morphology of spherolites (crystalline and amorphous phase). Degree of crystallinity was analysed by differential scanning calorimetry (DSC) and wide angle X-ray scattering (WAXS). Selected samples surfaces were O2 plasma treated to decrease hydrophobic properties of PCL. L929 cells adhesion and morphology were analyzed by immunohistochemical staining for actin and nuc lei. Cell activity and proliferation were analyzed. Morphology of spherolites was analyses using interfered-polarization microscopy (MIP). Analyses indicate changes in spherolites shape, size and also crystalline/ amorphous phase amount. Differences of crystallinity for PCL using different molecular weight were analysed by DSC and WAXS measurements. Decrease of contact angle was observed for O2 plasma treated samples. All PCL films were found as nontoxic for L929 cells. Differences in cells spreading, activity and proliferation degree were found. Modification of Mn, solvent and concentration of PCL enable film formation in wide range of crystallinity. L929 during in-vitro study interact with the PCL film. Crystallinity as part of the supermolecular structure influence on cells morphology.

Keywords:

crystallinity, PCL, cellular responce, structure

Affiliations:
Kołbuk D.-IPPT PAN
Denis P.-IPPT PAN
Urbanek O.-IPPT PAN
11.Urbanek O., Kucharska M., Dulnik J., Kołbuk D., Bicomponent nanofibers in tissue engineering, Szkoła Zimowa, 2014-12-15/12-16, Warszawa (PL), pp.30, 2014
Abstract:

Bicomponent poly(caprolactone)/ chitosan (PCL/Chit) nanofibers are a promising alternative for cartilage tissue regeneration. Chitosan is characterized by high structural similarity to the glycosaminoglycans (GAG) which naturally occur in the extracellular matrix (ECM). Its hydrophilicity is beneficial for cells adhesion and proliferation [1]. The amino groups in chitosan are responsible for the formation of polycations, which subsequently form compounds with natural and synthetic anions (proteins, lipids, synthetic polymers which are negatively charged) [2, 3]. Electrospinning of polycations with positive charge on the needle, is difficult due to the instability of the stream resulting from large repulsion force in the polymer jet [3]. Introduction of synthetic polymer molecules to the solution decreases interactions between the chains of chitosan and reduces the viscosity of the solution, so they are easier to form by electrospinning, as well as with negative charge on the needle [4 ]. A synthetic polymer, which is poly(caprolactone), improves mechanical properties of the fibers and the time of the hydrolytic degradation of the scaffold [4]. Nanofibers are excellent material for cell scaffolds used in tissue engineering because of high similarity of their morphology to native extracellular matrix (ECM) [1, 2]. From the viewpoint of cartilage tissue regeneration scaffold in the form of nanofibers is particularly justified due to naturally occurring network of polymer fibers (proteins and glycosaminoglycans) called aggrecans, in ECM of cartilage. Chondrocytes are connected with aggrecans [4]. Both, the structure and composition of formed nanofibers may affect the time in which cells will reach their proper morphology and undertake its functions [4]. In order to study cell behavior on electrospun PCL/chitosan nonwoven, fibroblasts L929 were cultured. Actin Green staining was conducted in order to imagine actin cytoskeleton of fibroblasts. To characterize, both fibers structure and cell morphology, SEM imagining was done. AFM imaging was carried to describe fibers topography and phase distribution. Also conductivity and viscosity of the PCL/chitosan solution with various polymer ratio was measured.

Keywords:

electrospinninig, scaffolds, PCL, chitozan, cellular responce

Affiliations:
Urbanek O.-IPPT PAN
Kucharska M.-IPPT PAN
Dulnik J.-IPPT PAN
Kołbuk D.-IPPT PAN