Olga Urbanek-Świderska, Ph.D., Eng.

Laboratory of Polymers and Biomaterials (SPPiB)
position: assistant professor
telephone: (+48) 22 826 12 81 ext.: 425
room: 338
e-mail: ourbanek

Doctoral thesis
2019-05-30Wpływ biegunowości napięcia w procesie elektroprzędzenia na wybrane właściwości i modyfikację powierzchni nanowłókien oraz odpowiedź komórkową 
supervisor -- Prof. Paweł Sajkiewicz, Ph.D., Dr. Habil., Eng., IPPT
supervisor -- Dorota Kołbuk-Konieczny, Ph.D., Eng., SPPiB/IPPT
1366
 
Recent publications
1.Pierini F., Guglielmelli A., Urbanek O., Nakielski P., Pezzi L., Buda R., Lanzi M., Kowalewski T.A., De Sio L., Thermoplasmonic‐activated hydrogel based dynamic light attenuator, Advanced Optical Materials, ISSN: 2195-1071, DOI: 10.1002/adom.202000324, pp.2000324-1-7, 2020
Abstract:

This work describes the morphological, optical, and thermo‐optical properties of a temperature‐sensitive hydrogel poly(N‐isopropylacrylamide‐co‐N‐isopropylmethacrylamide) [P(NIPAm‐co‐NIPMAm]) film containing a specific amount of gold nanorods (GNRs). The light‐induced thermoplasmonic heating of GNRs is used to control the optical scattering of an initially transparent hydrogel film. A hydrated P(NIPAm‐co‐NIPMAm) film is optically clear at room temperature. When heated to temperatures over 37 °C via light irradiation with a resonant source (λ = 810 nm) to the GNRs, a reversible phase transition from a swollen hydrated state to a shrunken dehydrated state occurs. This phenomenon causes a drastic and reversible change in the optical transparency from a clear to an opaque state. A significant red shift (≈30 nm) of the longitudinal band can also be seen due to an increased average refractive index surrounding the GNRs. This change is in agreement with an ad hoc theoretical model which uses a modified Gans theory for ellipsoidal nanoparticles. Morphological analysis of the composite film shows the presence of well‐isolated and randomly dispersed GNRs. Thermo‐optical experiments demonstrate an all‐optically controlled light attenuator (65% contrast ratio) which can be easily integrated in several modern optical applications such as smart windows and light‐responsive optical attenuators.

Keywords:

active plasmonics, gold nanorods, hydrogels, optical attenuators, optical transparency, plasmonic nanoparticles, polymers

Affiliations:
Pierini F.-IPPT PAN
Guglielmelli A.-University of Calabria (IT)
Urbanek O.-IPPT PAN
Nakielski P.-IPPT PAN
Pezzi L.-other affiliation
Buda R.-Institute of Physical Chemistry, Polish Academy of Sciences (PL)
Lanzi M.-University of Bologna (IT)
Kowalewski T.A.-IPPT PAN
De Sio L.-Sapienza University of Rome (IT)
2.Kołbuk D., Heljak M., Choińska E., Urbanek O., Novel 3D hybrid nanofiber scaffolds for bone regeneration, Polymers, ISSN: 2073-4360, DOI: 10.3390/polym12030544, Vol.12, No.3, pp.544-1-18, 2020
Abstract:

Development of hybrid scaffolds and their formation methods occupies an important place in tissue engineering. In this paper, a novel method of 3D hybrid scaffold formation is presented as well as an explanation of the differences in scaffold properties, which were a consequence of different crosslinking mechanisms. Scaffolds were formed from 3D freeze-dried gelatin and electrospun poly(lactide-co-glicolide) (PLGA) fibers in a ratio of 1:1 w/w. In order to enhance osteoblast proliferation, the fibers were coated with hydroxyapatite nanoparticles (HAp) using sonochemical processing. All scaffolds were crosslinked using an EDC/NHS solution. The scaffolds' morphology was imaged using scanning electron microscopy (SEM). The chemical composition of the scaffolds was analyzed using several methods. Water absorption and mass loss investigations proved a higher crosslinking degree of the hybrid scaffolds than a pure gelatin scaffold, caused by additional interactions between gelatin, PLGA, and HAp. Additionally, mechanical properties of the 3D hybrid scaffolds were higher than traditional hydrogels. In vitro studies revealed that fibroblasts and osteoblasts proliferated and migrated well on the 3D hybrid scaffolds, and also penetrated their structure during the seven days of the experiment.

Keywords:

hybrid scaffolds, electrospinning, freeze-drying, gelatin, hydroxyapatite, sonochemical covering/grafting

Affiliations:
Kołbuk D.-IPPT PAN
Heljak M.-Warsaw University of Technology (PL)
Choińska E.-Warsaw University of Technology (PL)
Urbanek O.-IPPT PAN
3.Pawłowska S., Rinoldi C., Nakielski P., Ziai Y., Urbanek O., Li X., Kowalewski T.A., Ding B., Pierini F., Ultraviolet light‐assisted electrospinning of core–shell fully cross‐linked P(NIPAAm‐co‐NIPMAAm) hydrogel‐based nanofibers for thermally induced drug delivery self‐regulation, Advanced Materials Interfaces, ISSN: 2196-7350, DOI: 10.1002/admi.202000247, Vol.7, No.12, pp.2000247-1-13, 2020
Abstract:

Body tissues and organs have complex functions which undergo intrinsic changes during medical treatments. For the development of ideal drug delivery systems, understanding the biological tissue activities is necessary to be able to design materials capable of changing their properties over time, on the basis of the patient's tissue needs. In this study, a nanofibrous thermal‐responsive drug delivery system is developed. The thermo‐responsivity of the system makes it possible to self‐regulate the release of bioactive molecules, while reducing the drug delivery at early stages, thus avoiding high concentrations of drugs which may be toxic for healthy cells. A co‐axial electrospinning technique is used to fabricate core–shell cross‐linked copolymer poly(N‐isopropylacrylamide‐co‐N‐isopropylmethacrylamide) (P(NIPAAm‐co‐NIPMAAm)) hydrogel‐based nanofibers. The obtained nanofibers are made of a core of thermo‐responsive hydrogel containing a drug model, while the outer shell is made of poly‐l‐lactide‐co‐caprolactone (PLCL). The custom‐made electrospinning apparatus enables the in situ cross‐linking of P(NIPAAm‐co‐NIPMAAm) hydrogel into a nanoscale confined space, which improves the electrospun nanofiber drug dosing process, by reducing its provision and allowing a self‐regulated release control. The mechanism of the temperature‐induced release control is studied in depth, and it is shown that the system is a promising candidate as a "smart" drug delivery platform.

Keywords:

biomimetic nanomaterials, electrospun core–shell nanofibers, hierarchical nanostructures, smart drug delivery, thermo‐responsive hydrogels

Affiliations:
Pawłowska S.-IPPT PAN
Rinoldi C.-IPPT PAN
Nakielski P.-IPPT PAN
Ziai Y.-IPPT PAN
Urbanek O.-IPPT PAN
Li X.-Donghua University (CN)
Kowalewski T.A.-IPPT PAN
Ding B.-Donghua University (CN)
Pierini F.-IPPT PAN
4.Zaszczyńska A., Sajkiewicz P.Ł., Gradys A., Tymkiewicz R., Urbanek O., Kołbuk D., Influence of process-material conditions on the structure and biological properties of electrospun polyvinylidene fluoride fibers, BULLETIN OF THE POLISH ACADEMY OF SCIENCES: TECHNICAL SCIENCES, ISSN: 0239-7528, DOI: 10.24425/bpasts.2020.133368, Vol.68, No.3, pp.627-633, 2020
Abstract:

Polyvinylidene fluoride (PVDF) is one of the most important piezoelectric polymers. Piezoelectricity in PVDF appears in polar β and ɣ phases. Piezoelectric fibers obtained by means of electrospinning may be used in tissue engineering (TE) as a smart analogue of the natural extracellular matrix (ECM). We present results showing the effect of rotational speed of the collecting drum on morphology, phase content and in vitro biological properties of PVDF nonwovens. Morphology and phase composition were analyzed using scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (FTIR), respectively. It was shown that increasing rotational speed of the collector leads to an increase in fiber orientation, reduction in fiber diameter and considerable increase of polar phase content, both b and g. In vitro cell culture experiments, carried out with the use of ultrasounds in order to generate electrical potential via piezoelectricity, indicate a positive effect of polar phases on fibroblasts. Our preliminary results demonstrate that piezoelectric PVDF scaffolds are promising materials for tissue engineering applications, particularly for neural tissue regeneration, where the electric potential is crucial.

Keywords:

scaffolds, electrospinning, polyvinylidene fluoride, tissue engineering

Affiliations:
Zaszczyńska A.-IPPT PAN
Sajkiewicz P.Ł.-IPPT PAN
Gradys A.-IPPT PAN
Tymkiewicz R.-IPPT PAN
Urbanek O.-IPPT PAN
Kołbuk D.-IPPT PAN
5.Kołbuk D., Urbanek O., Denis P., Choińska E., Sonochemical coating as an effective method of polymeric nonwovens functionalization, Journal of Biomedical Materials Research Part A, ISSN: 1549-3296, DOI: 10.1002/jbm.a.36751, Vol.107, No.11, pp.2447-2457, 2019
Abstract:

A surface of polymeric nonwovens may be coated with various types of nanoparticles for medical applications, filtration, and so forth. However, quite often methods used for surface modification are difficult to scale up or are not applicable for polymers. In this article, we present one-step process enabling nonwovens functionalization. Poly(l-lactide-co-glicolide) (PLGA) nonwovens were prepared by electrospinning process and coated with hydroxyapatite nanoparticles (HAp) using ultrasonic processing. The effect of the process was evaluated with various techniques. HAp layer was successfully attached without loss of structural properties of HAp or PLGA nonwovens. The analysis confirmed the decrease of hydrophobicity of coated nonwoven, as well as its biocompatibility, making this material valuable from the perspective of medical applications. The sonochemical functionalization of polymeric nonwovens may be considered as an effective and economic method, enhancing surface properties of electrospun nonwovens for various applications.

Keywords:

lectrospinning, fibrous composites, nanoparticles, surface modification, ultrasonic treatment

Affiliations:
Kołbuk D.-IPPT PAN
Urbanek O.-IPPT PAN
Denis P.-IPPT PAN
Choińska E.-Warsaw University of Technology (PL)
6.Urbanek O., Kołbuk D., Wróbel M., Articular cartilage: new directions and barriers of scaffolds development – review, International Journal of Polymeric Materials and Polymeric Biomaterials, ISSN: 0091-4037, DOI: 10.1080/00914037.2018.1452224, Vol.68, No.7, pp.396-410, 2019
Abstract:

Despite progress which has been made in recent years in the field of cell-based therapies or cell scaffolds for cartilage regeneration, a lot of work still needs to be done. Scaffolds remain a great base for tissue regeneration. However, proper implantation procedures or post-treatment still await development. In this review we summarize paths of cartilage treatment, especially focusing on cell scaffold design and manufacture. As well as the advantages and disadvantages of available or investigated methods and materials, especially focusing on cartilage scaffold design. We show the most promising directions and barriers in the creation of healthy tissue.

Keywords:

cartilage regeneration, medical devices, scaffold development, tissue engineering

Affiliations:
Urbanek O.-IPPT PAN
Kołbuk D.-IPPT PAN
Wróbel M.-Centre for Specialized Surgery (PL)
7.Zaszczyńska A., Sajkiewicz P., Gradys A., Kołbuk D., Urbanek O., Cellular studies on piezoelectric polyvinylidene fluoride nanofibers subjected to ultrasounds stimulations, ENGINEERING OF BIOMATERIALS / INŻYNIERIA BIOMATERIAŁÓW, ISSN: 1429-7248, Vol.22, No.153, pp.25-25, 2019
8.Pierini F., Nakielski P., Urbanek O., Pawłowska S., Lanzi M., De Sio L., Kowalewski T.A., Polymer-Based Nanomaterials for Photothermal Therapy: From Light-Responsive to Multifunctional Nanoplatforms for Synergistically Combined Technologies, BIOMACROMOLECULES, ISSN: 1525-7797, DOI: 10.1021/acs.biomac.8b01138, Vol.19, No.11, pp.4147-4167, 2018
Abstract:

Materials for the treatment of cancer have been studied comprehensively over the past few decades. Among the various kinds of biomaterials, polymer-based nanomaterials represent one of the most interesting research directions in nanomedicine because their controlled synthesis and tailored designs make it possible to obtain nanostructures with biomimetic features and outstanding biocompatibility. Understanding the chemical and physical mechanisms behind the cascading stimuli-responsiveness of smart polymers is fundamental for the design of multifunctional nanomaterials to be used as photothermal agents for targeted polytherapy. In this review, we offer an in-depth overview of the recent advances in polymer nanomaterials for photothermal therapy, describing the features of three different types of polymer-based nanomaterials. In each case, we systematically show the relevant benefits, highlighting the strategies for developing light-controlled multifunctional nanoplatforms that are responsive in a cascade manner and addressing the open issues by means of an inclusive state-of-the-art review. Moreover, we face further challenges and provide new perspectives for future strategies for developing novel polymeric nanomaterials for photothermally assisted therapies.

Affiliations:
Pierini F.-IPPT PAN
Nakielski P.-IPPT PAN
Urbanek O.-IPPT PAN
Pawłowska S.-IPPT PAN
Lanzi M.-University of Bologna (IT)
De Sio L.-Sapienza University of Rome (IT)
Kowalewski T.A.-IPPT PAN
9.Urbanek O., Pierini F., Choińska E., Sajkiewicz P., Bil M., Święszkowski W., Effect of hydroxyapatite nanoparticles addition on structure properties of poly(L-lactide-co-glycolide) after gamma sterilization, Polymer Composites, ISSN: 0272-8397, DOI: 10.1002/pc.24028, Vol.39, No.4, pp.1023-1031, 2018
Abstract:

Physical and chemical factors resulting from the sterilization methods may affect the structure and properties of the materials which undergo this procedure. Poly(l-lactide-co-glicolide) (PLGA) is commonly used for medical applications, but, due to its inadequate mechanical properties, it is not recommended for load-bearing applications. One of the methods for improving PLGA mechanical properties is addition of hydroxyapatite nanoparticles (nHAp). The aim of this study was to evaluate the effect of nanoparticles addition on PLGA structure and properties after gamma radiation. According to our results, reduction of the molecular mass caused by gamma radiation was lower for PLGA with nHAp addition. Differential scanning calorimetry (DSC) analysis indicates an increase of crystallinity caused both by nHAp and gamma radiation. The first phenomenon can be explained by heteronucleation, while the second one is most probably related to higher molecular mobility of degrading polymer. Moreover, addition of nanoparticles increases thermal stability and affects the Young's modulus changes after gamma radiation.

Affiliations:
Urbanek O.-IPPT PAN
Pierini F.-IPPT PAN
Choińska E.-Warsaw University of Technology (PL)
Sajkiewicz P.-IPPT PAN
Bil M.-Warsaw University of Technology (PL)
Święszkowski W.-other affiliation
10.Pierini F., Lanzi M., Nakielski P., Pawłowska S., Urbanek O., Zembrzycki K., Kowalewski T.A., Single-Material Organic Solar Cells Based on Electrospun Fullerene-Grafted Polythiophene Nanofibers, Macromolecules, ISSN: 0024-9297, DOI: 10.1021/acs.macromol.7b00857, Vol.50, No.13, pp.4972-4981, 2017
Abstract:

Highly efficient single-material organic solar cells (SMOCs) based on fullerene-grafted polythiophenes were fabricated by incorporating electrospun one-dimensional (1D) nanostructures obtained from polymer chain stretching. Poly(3-alkylthiophene) chains were chemically tailored in order to reduce the side effects of charge recombination which severely affected SMOC photovoltaic performance. This enabled us to synthesize a donor–acceptor conjugated copolymer with high solubility, molecular weight, regioregularity, and fullerene content. We investigated the correlations among the active layer hierarchical structure given by the inclusion of electrospun nanofibers and the solar cell photovoltaic properties. The results indicated that SMOC efficiency can be strongly increased by optimizing the supramolecular and nanoscale structure of the active layer, while achieving the highest reported efficiency value (PCE = 5.58%). The enhanced performance may be attributed to well-packed and properly oriented polymer chains. Overall, our work demonstrates that the active material structure optimization obtained by including electrospun nanofibers plays a pivotal role in the development of efficient SMOCs and suggests an interesting perspective for the improvement of copolymer-based photovoltaic device performance using an alternative pathway.

Affiliations:
Pierini F.-IPPT PAN
Lanzi M.-University of Bologna (IT)
Nakielski P.-IPPT PAN
Pawłowska S.-IPPT PAN
Urbanek O.-IPPT PAN
Zembrzycki K.-IPPT PAN
Kowalewski T.A.-IPPT PAN
11.Urbanek O., Sajkiewicz P., Pierini F., The effect of polarity in the electrospinning process on PCL/chitosan nanofibres' structure, properties and efficiency of surface modification, POLYMER, ISSN: 0032-3861, DOI: 10.1016/j.polymer.2017.07.064, Vol.124, pp.168-175, 2017
Abstract:

The aim of this research was to study the effect of charge polarity applied to the spinning nozzle on the structure and properties of polycaprolactone/chitosan (PCL/CHT) blends, in particular the efficiency of further surface modification by chondroitin sulphate (CS). The observed differences in the morphology and properties of fibres formed at different polarities were interpreted in terms of molecular interactions occurring in the system. FTIR results indicate stronger PCL-chitosan interactions at negative polarity, resulting in lower PCL crystallinity and crystal size distribution determined by DSC, as well as lower wettability. The charge polarity influences PCL/CHT fibre morphology and tailors some of their properties, e.g. wettability, mechanical properties and the efficiency of surface modification. Better efficiency of CS attachment was observed at negative polarity using atomic force microscopy (AFM), and X-ray photoelectron spectroscopy (XPS) is most probably related to higher chitosan content at the fibres' surface being attracted by the negative external potential.

Keywords:

Polycaprolactone/chitosan nanofibres, Charge potential effect in electrospinning, Polycaprolactone-chitosan interactions

Affiliations:
Urbanek O.-IPPT PAN
Sajkiewicz P.-IPPT PAN
Pierini F.-IPPT PAN
12.Urbanek O., Sajkiewicz P., Pierini F., Czerkies M., Kołbuk D., Structure and properties of polycaprolactone/chitosan nonwovens tailored by solvent systems, Biomedical Materials, ISSN: 1748-6041, DOI: 10.1088/1748-605X/aa5647, Vol.12, No.1, pp.015020-1-12, 2017
Abstract:

Electrospinning of chitosan blends is a reasonable idea to prepare fibre mats for biomedical applications. Synthetic and natural components provide, for example, appropriate mechanical strength and biocompatibility, respectively. However, solvent characteristics and the polyelectrolyte nature of chitosan influence the spinnability of these blends. In order to compare the effect of solvent on polycaprolactone/chitosan fibres, two types of the most commonly used solvent systems were chosen, namely 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) and acetic acid (AA)/formic acid (FA). Results obtained by various experimental methods clearly indicated the effect of the solvent system on the structure and properties of electrospun polycaprolactone/chitosan fibres. Viscosity measurements confirmed different polymer–solvent interactions. Various molecular interactions resulting in different macromolecular conformations of chitosan influenced its spinnability and properties. HFIP enabled fibres to be obtained whose average diameter was less than 250 nm while maintaining the brittle and hydrophilic character of the nonwoven, typical for the chitosan component. Spectroscopy studies revealed the formation of chitosan salts in the case of the AA/FA solvent system. Chitosan salts visibly influenced the structure and properties of the prepared fibre mats. The use of AA/FA caused a reduction of Young's modulus and wettability of the proposed blends. It was confirmed that wettability, mechanical properties and the antibacterial effect of polycaprolactone/chitosan fibres may be tailored by selecting an appropriate solvent system. The MTT cell proliferation assay revealed an increase of cytotoxicity to mouse fibroblasts in the case of 25% w/w of chitosan in electrospun nonwovens.

Keywords:

chitosan, electrospinning, PCL/chitosan fibres, solvent system, chitosan salts

Affiliations:
Urbanek O.-IPPT PAN
Sajkiewicz P.-IPPT PAN
Pierini F.-IPPT PAN
Czerkies M.-IPPT PAN
Kołbuk D.-IPPT PAN
13.Mayerberger E.A., Urbanek O., McDaniel R.M., Street R.M., Barsoum M.W., Schauer C.L., Preparation and characterization of polymer-Ti3C2Tx(MXene) composite nanofibers produced via electrospinning, JOURNAL OF APPLIED POLYMER SCIENCE, ISSN: 0021-8995, DOI: 10.1002/app.45295, Vol.134, No.37, pp.45295-1-7, 2017
Abstract:

MXene, a recently-discovered family of two-dimensional (2 D) transition metal carbides and/or nitrides, have attracted much interest because of their unique electrical, thermal, and mechanical properties. In this study, poly(acrylic acid) (PAA), polyethylene oxide (PEO), poly(vinyl alcohol) (PVA), and alginate/PEO were electrospun with delaminated Ti3C2 (MXene) flakes. The effect of small additions of delaminated Ti3C2 (1% w/w) on the structure and properties of the nanofibers were investigated and compared with those of the neat polymer nanofibers using scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FT-IR). Ti3C2 had an effect on the solution properties of the polymer and a greater effect on the average fiber diameter. The Ti3C2Tx/PEO solution exhibited the largest change in viscosity and conductivity with an 11% and 73.6% increase over the base polymer, respectively. X-ray diffractograms demonstrated a high degree of crystallization for Ti3C2/PEO and a slight decrease in crystallinity for Ti3C2/PVA.

Keywords:

composite nanofibers, electrospinning, MXene

Affiliations:
Mayerberger E.A.-Drexel University (US)
Urbanek O.-IPPT PAN
McDaniel R.M.-Drexel University (US)
Street R.M.-Drexel University (US)
Barsoum M.W.-Drexel University (US)
Schauer C.L.-Drexel University (US)

Conference abstracts
1.Urbanek-Świderska O., Kołbuk D., Designing of Three-Dimensional Hybrid Scaffolds for Tissue Regeneration, 2019 MRS FALL MEETING & EXHIBIT, 2019-12-01/12-06, Boston (US), pp.1659, 2019
2.Zaszczyńska A., Sajkiewicz P., Gradys A., Kołbuk D., Urbanek O., Cellular studies of piezoelectric nanofibers with ultrasound stimulations, Aerogels Processing, Modelling and Environmental-Driven Applications, 2019-10-21/10-23, Coimbra (PT), No.P04, pp.36, 2019
3.Zaszczyńska A., Sajkiewicz P., Gradys A., Urbanek O., Kołbuk D., Influence of process-material conditions on the phase composition, architecture and biological properties of electrospun polyvinylidene fluoride fibers, CNM 2019, 6th CONFERENCE ON NANO- AND MICROMECHANICS, 2019-07-03/07-05, Rzeszów (PL), pp.145-147, 2019
Keywords:

scaffolds, electrospinning, polyvinylidene fluoride, tissue engineering

Affiliations:
Zaszczyńska A.-IPPT PAN
Sajkiewicz P.-IPPT PAN
Gradys A.-IPPT PAN
Urbanek O.-IPPT PAN
Kołbuk D.-IPPT PAN
4.Nakielski P., Urbanek O., Pawłowska S., Kowalewski T.A., Pierini F., Externally triggered on-demand drug release from stimuli-responsive hydrogel-based electrospun nanofibers and their composites, Electrospin 2019, 6th International Conference on Electrospinning 2019, 2019-06-19/06-21, Shanghai (CN), pp.63-63, 2019
Abstract:

Pulsatile drug delivery systems are gaining a lot of interest because of their numerous advantages, especially when compared to conventional pharmaceutical dosage forms [1]. These materials are time- and site-specific drug delivery systems which can minimize deleterious side effects of conventional drug administration systems. Nevertheless, the delivery systems that are of particular interest are the ones with reversible on-off switching capability, because they allow the delivery of therapeutic agents at the proper time after a predetermined lag time. Among the polymers used for biomedical applications, hydrogels are a class of materials of particular significance, because they can provide spatial and temporal control over the release of various types of drugs. Stimuli-responsive hydrogels can release drugs on-demand with a fast release rate through different mechanisms. The effectiveness of this process can be maximized using nanostructured materials with a large surface-area-to-volume ratio such as electrospun nanofibers. Current challenges in the development of hydrogel electrospun fibrous nanomaterials lie in the lack of spinnability of pure hydrogel precursor solutions. Addressing this issue, we firstly designed a new core-shell nanofibrous material in which the poly(N-isopropylacrylamide)-derivative hydrogel is confined within a shell of a spinnable polymer (Figure 1a). Alternatively, we developed a scaffold material in which electrospun nanofibers loaded with different bioactive molecules where surrounded by a stimuli-responsive hydrogel (Figure 1b). Morphological and chemical characterization as well as drug release studies were carried out to confirm the material’s ability to supply different doses of drugs on demand and to study the release mechanism.

Affiliations:
Nakielski P.-IPPT PAN
Urbanek O.-IPPT PAN
Pawłowska S.-IPPT PAN
Kowalewski T.A.-IPPT PAN
Pierini F.-IPPT PAN
5.Nakielski P., Pawłowska S., Urbanek-Świderska O., Woźniak-Jezierska K., Barczewska M., Maksymowicz W., Injectable scaffolds for tissue engineering, ISSCR 2019, International Society for Stem Cell Research Annual Meeting, 2019-06-26/06-29, Los Angeles (US), pp.277-277, 2019
Abstract:

Intervertebral disc diseases are a significant medical problem affecting many people around the world. In Poland, the statistics of the Social Insurance Institution (Medical Abuse in 2016) indicate that low back pains and other intervertebral disc diseases constitute 17% of the total number of days of sick leave. In connection with the above, current work describes design of a composite scaffold as a carrier in cell therapy, which will contribute to the regeneration of the intervertebral disc, including the increase of its height. Our composite scaffold include nanofibers that were prepared with the use of the electrospinning method. This method is a simple but powerful technique for fabricating desirable nano- and microfibers by using a high potential electric field. Human Mesenchymal stem cells (MSCs) were cultured on the scaffold from poly(L-lactide). Proliferation kits and fluorescence microscopy were used to asses cells’ viability and adherence to the nanofibers’ surface. hMSCs were efficiently cultured on the nanofibrous scaffold. Cells could be readily detected in porous structure of the scaffold after 7 and 14 days of culture. Viability and proliferation kits proved that the material is not toxic. Drug release from nanofibrous material of model growth factor was conducted with pharmacopeia protocols. Drug release of the 14 kDa growth factor was achieved for 14 days without burst release. Nanofibrous biomaterials prove their advances in many tissue engineering applications. Adjustable porosity of the scaffold and the biocompability of biomaterial make it perfect candidate for cells’ scaffold in many medical procedures and also as a drug release carrier. With the use of single nanofibers, such biomaterials can also be readily used in minimally invasive procedures to regenerate IVD.

Keywords:

nanofibers, IVD, MSC

Affiliations:
Nakielski P.-IPPT PAN
Pawłowska S.-IPPT PAN
Urbanek-Świderska O.-IPPT PAN
Woźniak-Jezierska K.-University of Warmia and Mazury in Olsztyn (PL)
Barczewska M.-University of Warmia and Mazury in Olsztyn (PL)
Maksymowicz W.-University of Warmia and Mazury in Olsztyn (PL)
6.Nakielski P., De Sio L., Buda R., Guglielmelli A., Pawłowska S., Urbanek O., Kowalewski T.A., Pierini F., Photo-responsive PNIPAM-Gold Nanorods Hydrogel For Biomedical Applications, NOMA2019, The 14th Mediterranean Workshop and Topical Meeting, 2019-06-02/06-08, Cetraro (IT), pp.80-80, 2019
Abstract:

Stimuli-responsive drug delivery systems are gaining a lot of interest due to their numerous advantages, especially when compared to conventional pharmaceutical dosage forms. One of the examples is photo stimulation that together with nanometer size agents, having high absorption in the near-infrared region, generate heat due to the interaction with light. Stimuli-responsive hydrogels with gold nanorods (AuNRs), that are used as photothermal converters, can aid in releasing drugs on-demand with a fast release rate through different mechanisms. Here we report an easy method for preparing AuNRs encapsulated in a poly(N-isopropylacrylamide) (PNIPAm) hydrogel that release water-soluble drugs due to photo stimulation. PNIPAm-AuNRs demonstrated remote, pulsatile drug release and ex vivo action after irradiation using a NIR laser. Morphological and chemical characterization as well as drug release studies were carried out to confirm the material’s ability to supply different doses of drugs on demand and to study the release mechanism. By combining the photothermal property of AuNRs and thermal-responsive effect of PNIPAm, the hydrogel shows fast thermal/photoresponse, high heating rate, high structural integrity and increased drug release due to phase change mechanism.

Keywords:

drug delivery systems, nanofibers

Affiliations:
Nakielski P.-IPPT PAN
De Sio L.-Sapienza University of Rome (IT)
Buda R.-Institute of Physical Chemistry, Polish Academy of Sciences (PL)
Guglielmelli A.-University of Calabria (IT)
Pawłowska S.-IPPT PAN
Urbanek O.-IPPT PAN
Kowalewski T.A.-IPPT PAN
Pierini F.-IPPT PAN
7.Pierini F., Lanzi M., Nakielski P., Pawłowska S., Urbanek O., Kowalewski T.A., Light-matter interaction in electrospun nanofibers: novel conjugated polymer-based one-dimensional nanostructures for organic solar cell applications, NOMA2019, The 14th Mediterranean Workshop and Topical Meeting, 2019-06-02/06-08, Cetraro (IT), pp.55-55, 2019
Abstract:

Single-material organic solar cells (SMOCs) based on fullerene-grafted polythiophenes are considered promising devices for organic solar cells (OSCs). The main efforts in this field focus on the chemical tailoring of polymer molecules to reduce the side effects of charge recombination. These advances have made it possible to obtain a power conversion efficiency (PCE) close to conventional bulk heterojunction (BHJ) cells. So far, however, SMOCs still show inadequate efficiencies due to ineffective charge transport. Here we show how SMOC efficiency can be strongly increased by optimizing the supramolecular and nanoscale structure of the active layer, while achieving the highest reported efficiency value (PCE = 5.58%) [1]. The enhanced performance may be attributed to well-packed and properly oriented polymer chains. The hierarchical structure is given by the incorporation of electrospun one-dimensional nanostructures obtained from polymer chain stretching. Our results suggest that the active material optimization obtained by the use of electrospun nanofibers plays a key role in the development of efficient SMOCs.

Affiliations:
Pierini F.-IPPT PAN
Lanzi M.-University of Bologna (IT)
Nakielski P.-IPPT PAN
Pawłowska S.-IPPT PAN
Urbanek O.-IPPT PAN
Kowalewski T.A.-IPPT PAN
8.Kołbuk-Konieczny D., Urbanek-Świderska O., Jeznach O., Hybrid scaffold to anterior cruciate ligament regeneration, TERMIS EU 2019, TERMIS European Chapter Meeting 2019, Tissue Engineering Therapies: From Concept to Clinical Translation & Commercialisation, 2019-05-27/05-31, Rodos (GR), pp.1461, 2019
9.Urbanek O., Sajkiewicz P., Pierini F., Cell response on fibres surface properties induced by process parameters and post-treatment of electrospun nonwovens, Electrospin2018 International Conference, 2018-01-16/01-18, Stellenbosch (ZA), pp.62-63, 2018
10.Pierini F., Lanzi M., Nakielski P., Pawłowska S., Urbanek O., Kowalewski T.A., Electrospinning of polythiophene with pendant fullerene nanofibers for single-material organic solar cells, Electrospin2018 International Conference, 2018-01-16/01-18, Stellenbosch (ZA), pp.19-20, 2018
11.Urbanek O., Kołbuk D., Sonochemical modification of electrospun fibres with hydroxyapatite nanoparticles, ESB2018, 29th European Conference on Biomaterials, 2018-09-09/09-13, Maastricht (NL), pp.523-524, 2018
12.Urbanek O., Sajkiewicz P., The effect of electrospinning parameters on selected properties of polyelectrolytes' fibres, PICETE, Polish-Israeli Conference on Electrospinning and Tissue Engineering, 2018-10-04/10-05, Warszawa (PL), pp.21, 2018
13.Urbanek O., Sajkiewicz P., Biomimetically surface modified fibres for cartilage regeneration, TERMIS-EU 2017, European Chapter Meeting of the Tissue Engineering and Regenerative Medicine International Society 2017, 2017-06-26/06-30, Davos (CH), pp.P666-P666, 2017
14.Kołbuk D., Denis P., Urbanek O., Tailoring of mechanical properties by molecular orientation in polymeric scaffolds, BioMaH, BIOMATERIALS FOR HEALTHCARE: Biomaterials for Tissue and Genetic Engineering and the Role of Nanotechnology, 2016-10-17/10-20, Rzym (IT), pp.443-446, 2016
Keywords:

molecular orientation, scaffolds, fibres, tissue engineering

Affiliations:
Kołbuk D.-IPPT PAN
Denis P.-IPPT PAN
Urbanek O.-IPPT PAN
15.Urbanek O., Sajkiewicz P., The effect of polarity on biomimetic surface modification of PCL/chitosan nanofibers formed by electrospinning, ELECTROSPIN 2016, 4th International Conference on Electrospinning, 2016-06-28/07-01, Otranto (IT), pp.1, 2016
16.Urbanek O., Sajkiewicz P., Schauer C., Charge Assisted Tailoring and its Effect on Surface Modification of Chitosan Nanofibers, Fiber Society 2016 Fall Meeting and Technical Conference, 2016-10-10/10-12, Ithaca (US), pp.1, 2016
17.Kowalczyk T., Cwiek K., Urbanek O., Kloskowski T., Pokrywczyńska M., Jundziłł A., Adamowicz J., Zabost E., Noszczyk B., Drewa T., Electrospun micro and nanofibers applied for animal models in urology and wound dressing. Potential applications in cancer treatment, 2nd INTERNATIONAL CONFERENCE ON BIO-BASED POLYMERS AND COMPOSITES, 2014-08-24/08-28, Visegrad (HU), pp.24, 2015
Abstract:

We used the principles of electrospinning to produce materials for applications in regenerative medicine of urinary bladder wall, ureter, wound dressing and potential applications in cancer therapy. Our research is based on biodegradable polymers produced by ring-opening polymerization. Scaffolds of poly(L-lactide-co-caprolactone) (PLCL) gradually degrade leaving no artificial material behind to be replaced by natural extracellular collagen matrix. We formed flat membranes of micro- and nanofibers to carry out regeneration of urinary bladder wall as animal model of cancer treatment. Grafts were tested for biocompatibility and aimed for guided cell growth, yet we were unsuccessful in mechanical compliance of nanomaterial and reconstructed tissue. We tested tubular scaffolds made of nanofibers aimed for ureter tissue engineering. We found stem cells seeding unnecessary. The results of nanomaterial implantation on animal model were better than for collagen matrices. Animal model was also tested for use of nanofibers of human serum albumin as wound dressing. The native structure of the protein was retained to maintain its anti-adhesive properties, despite poor mechanical characteristics. Nanomaterial caused no inflammation and was resorbed during 16 days. Last application of presented materials was targeted drug delivery system made of PLCL nanofibers. Release of anticancer drug complexed with nanoparticles is to be triggered by tumor cells. Such nanomaterial is potential drug delivery system. Acknowledgements: The authors wishes to thank for the cooperation: T. Chmielewski, P. Nakielski, K. Zembrzycki, G. Mikulowski and prof. T. A. Kowalewski from IPPT PAN. The project was partially supported by the National Centre for Research and Development. Grant No. STRATEGMED1/235368/8/NCBR/2014.

Keywords:

electrospinning, nanofibers, regenerative medicine, wound dressing, urology, biodegradable polymers, animal model.

Affiliations:
Kowalczyk T.-IPPT PAN
Cwiek K.-other affiliation
Urbanek O.-IPPT PAN
Kloskowski T.-other affiliation
Pokrywczyńska M.-other affiliation
Jundziłł A.-other affiliation
Adamowicz J.-Nicolaus Copernicus University (PL)
Zabost E.-University of Warsaw (PL)
Noszczyk B.-Medical University of Warsaw (PL)
Drewa T.-Nicolaus Copernicus University (PL)
18.Urbanek O., Pierini F., Kołbuk D., Sajkiewicz P., Effect of positive and negative charge applied during electrospinning on surface chemistry and selected properties of chitosan nanofibers, COST MP1206 Workshop on Electrospinning of Chitosan, 2015-09-02/09-03, Münster (DE), pp.7-8, 2015
Keywords:

electrospinning, fibres, surface, chitosan

Affiliations:
Urbanek O.-IPPT PAN
Pierini F.-IPPT PAN
Kołbuk D.-IPPT PAN
Sajkiewicz P.-IPPT PAN
19.Urbanek o., Kołbuk D., Sajkiewicz P., Cellular Response on Polycaprolactone/Chitosan Crystalinity Tailored by Solvent System in Electrospinning Process, PUZZLE 2015, IV Wrocławska Konferencja Studentów Nauk Technicznych i Ścisłych, 2015-04-18/04-19, Wrocław (PL), pp.22, 2015
Keywords:

chitosan, PCL, cellular responce, electrospinning

Affiliations:
Urbanek o.-IPPT PAN
Kołbuk D.-IPPT PAN
Sajkiewicz P.-IPPT PAN
20.Urbanek O., Kołbuk D., Sajkiewicz P., Odpowiedź komórkowa na krystaliczność włókien polikaprolakton/ chitozan uzyskiwaną z wybranych rozpuszczalników, XIX Krajowa Konferencja Biocybernetyka i Inżynieria Biomedyczna, 2015-10-14/10-16, Warszawa (PL), pp.213, 2015
Keywords:

chitozan, polikaprolakton, electrospinning, L929, cytotoksyczność

Affiliations:
Urbanek O.-IPPT PAN
Kołbuk D.-IPPT PAN
Sajkiewicz P.-IPPT PAN
21.Urbanek O., Bil M., Święszkowski W., The effect of hydroxyapatite nanoparticles addition on crystallinity and surface properties of bioresorbable nanocomposites after gamma sterylization treatment, 4th Summer Symposium on Nanomaterials and Their Application to Biology and Medicine, 2014-06-15/06-18, Poznań (PL), pp.1, 2014
22.Kołbuk D., Denis P., Urbanek O., L929 response on polycaprolactone films with tailored crystallinity, Szkoła Zimowa, 2014-12-15/12-16, Warszawa (PL), pp.29, 2014
Abstract:

Influence of the crystallinity of the substrate on cell proliferation during in-vitro study was highlighted in few articles. Methods of forming 3-D scaffolds usually do not take into account crystallinity optimisation. The aim of proposed presentation is to investigate an influence of polycaprolactone (PCL) crystallinity on cells spreading, their activity and proliferation. PCL Mn 45k and Mn 80k g/mol were used. As a solvents: HFIP (H) and Acetic Acid (AA) were used. Two methods of foil preparation were analysed: -forming from melt (PCL45, PCL80) -forming from 10%wt solution (e.g. PH45, PAA45) Samples were analyzed using interfered-polarization microscopy (MIP) which allows to describe the morphology of spherolites (crystalline and amorphous phase). Degree of crystallinity was analysed by differential scanning calorimetry (DSC) and wide angle X-ray scattering (WAXS). Selected samples surfaces were O2 plasma treated to decrease hydrophobic properties of PCL. L929 cells adhesion and morphology were analyzed by immunohistochemical staining for actin and nuc lei. Cell activity and proliferation were analyzed. Morphology of spherolites was analyses using interfered-polarization microscopy (MIP). Analyses indicate changes in spherolites shape, size and also crystalline/ amorphous phase amount. Differences of crystallinity for PCL using different molecular weight were analysed by DSC and WAXS measurements. Decrease of contact angle was observed for O2 plasma treated samples. All PCL films were found as nontoxic for L929 cells. Differences in cells spreading, activity and proliferation degree were found. Modification of Mn, solvent and concentration of PCL enable film formation in wide range of crystallinity. L929 during in-vitro study interact with the PCL film. Crystallinity as part of the supermolecular structure influence on cells morphology.

Keywords:

crystallinity, PCL, cellular responce, structure

Affiliations:
Kołbuk D.-IPPT PAN
Denis P.-IPPT PAN
Urbanek O.-IPPT PAN
23.Urbanek O., Kucharska M., Dulnik J., Kołbuk D., Bicomponent nanofibers in tissue engineering, Szkoła Zimowa, 2014-12-15/12-16, Warszawa (PL), pp.30, 2014
Abstract:

Bicomponent poly(caprolactone)/ chitosan (PCL/Chit) nanofibers are a promising alternative for cartilage tissue regeneration. Chitosan is characterized by high structural similarity to the glycosaminoglycans (GAG) which naturally occur in the extracellular matrix (ECM). Its hydrophilicity is beneficial for cells adhesion and proliferation [1]. The amino groups in chitosan are responsible for the formation of polycations, which subsequently form compounds with natural and synthetic anions (proteins, lipids, synthetic polymers which are negatively charged) [2, 3]. Electrospinning of polycations with positive charge on the needle, is difficult due to the instability of the stream resulting from large repulsion force in the polymer jet [3]. Introduction of synthetic polymer molecules to the solution decreases interactions between the chains of chitosan and reduces the viscosity of the solution, so they are easier to form by electrospinning, as well as with negative charge on the needle [4 ]. A synthetic polymer, which is poly(caprolactone), improves mechanical properties of the fibers and the time of the hydrolytic degradation of the scaffold [4]. Nanofibers are excellent material for cell scaffolds used in tissue engineering because of high similarity of their morphology to native extracellular matrix (ECM) [1, 2]. From the viewpoint of cartilage tissue regeneration scaffold in the form of nanofibers is particularly justified due to naturally occurring network of polymer fibers (proteins and glycosaminoglycans) called aggrecans, in ECM of cartilage. Chondrocytes are connected with aggrecans [4]. Both, the structure and composition of formed nanofibers may affect the time in which cells will reach their proper morphology and undertake its functions [4]. In order to study cell behavior on electrospun PCL/chitosan nonwoven, fibroblasts L929 were cultured. Actin Green staining was conducted in order to imagine actin cytoskeleton of fibroblasts. To characterize, both fibers structure and cell morphology, SEM imagining was done. AFM imaging was carried to describe fibers topography and phase distribution. Also conductivity and viscosity of the PCL/chitosan solution with various polymer ratio was measured.

Keywords:

electrospinninig, scaffolds, PCL, chitozan, cellular responce

Affiliations:
Urbanek O.-IPPT PAN
Kucharska M.-IPPT PAN
Dulnik J.-IPPT PAN
Kołbuk D.-IPPT PAN

Patents
Filing No./Date
Filing Publication
Autor(s)
Title
Protection Area, Applicant Name
Patent Number
Date of Grant
pdf
425049
2018-03-27
BUP 21/19
2019-10-07
Kołbuk-Konieczny D., Urbanek-Świderska O., Przybysz S., Gloc M.
Sposób obróbki powierzchni oraz implant wytworzony z wykorzystaniem obróbki powierzchni
PL, Instytut Podstawowych Problemów Techniki PAN
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