Beata Niemczyk-Soczyńska, M.Sc., Eng.

Laboratory of Polymers and Biomaterials (SPPiB)
position: doctoral student
telephone: (+48) 22 826 12 81 ext.: 171
room: 334
e-mail: bniem

Recent publications
1.Niemczyk-Soczyńska B., Gradys A., Kołbuk D., Krzton-Maziopa A., Sajkiewicz P., Crosslinking Kinetics of Methylcellulose Aqueous Solution and Its Potential as a Scaffold for Tissue Engineering, Polymers, ISSN: 2073-4360, DOI: 10.3390/polym11111772, Vol.11, No.1772, pp.1-17, 2019
Abstract:

Thermosensitive, physically crosslinked injectable hydrogels are in the area of interests of various scientific fields. One of the representatives of this materials group is an aqueous solution of methylcellulose. At ambient conditions, methylcellulose (MC) is a sol while on heating up to 37 °C, MC undergoes physical crosslinking and transforms into a gel. Injectability at room temperature, and crosslinkability during subsequent heating to physiological temperature raises hopes, especially for tissue engineering applications. This research work aimed at studying crosslinking kinetics, thermal, viscoelastic, and biological properties of MC aqueous solution in a broad range of MC concentrations. It was evidenced by Differential Scanning Calorimetry (DSC) that crosslinking of MC is a reversible two-stage process, manifested by the appearance of two endothermic effects, related to the destruction of water cages around methoxy groups, followed by crosslinking via the formation of hydrophobic interactions between methoxy groups in the polymeric chains. The DSC results also allowed the determination of MC crosslinking kinetics. Complementary measurements of MC crosslinking kinetics performed by dynamic mechanical analysis (DMA) provided information on the final storage modulus, which was important from the perspective of tissue engineering applications. Cytotoxicity tests were performed using mouse fibroblasts and showed that MC at low concentration did not cause cytotoxicity. All these efforts allowed to assess MC hydrogel relevance for tissue engineering applications.

Keywords:

methylcellulose, thermosensitive hydrogel, crosslinking kinetics, DSC, DMA, cellular tests

Affiliations:
Niemczyk-Soczyńska B.-IPPT PAN
Gradys A.-IPPT PAN
Kołbuk D.-IPPT PAN
Krzton-Maziopa A.-Warsaw University of Technology (PL)
Sajkiewicz P.-IPPT PAN
2.Niemczyk B., Sajkiewicz P.Ł., Kołbuk D., Injectable hydrogels as novel materials for central nervous system regeneration, Journal of neural engineering, ISSN: 1741-2560, DOI: 10.1088/1741-2552/aacbab, Vol.15, No.5, pp.051002-1-15, 2018
Abstract:

Approach. Injuries of the central nervous system (CNS) can cause serious and permanent disability due to limited regeneration ability of the CNS. Presently available therapies are focused on lesion spreading inhibition rather than on tissue regeneration. Recent investigations in the field of neural tissue engineering indicate extremely promising properties of novel injectable and non-injectable hydrogels which are tailored to serve as biodegradable scaffolds for CNS regeneration. Objective. This review discusses the state-of-the-art and barriers in application of novel polymer-based hydrogels without and with nanoparticles for CNS regeneration. Main results. Pure hydrogels suffer from lack of similarities to natural neural tissue. Many of the biological studies indicated nano-additives in hydrogels may improve their topography, mechanical properties, electroconductivity and biological functions. The most promising biomaterials which meet the requirements of CNS tissue engineering seem to be injectable thermosensitive hydrogels loaded with specific micro-and nanoparticles. Significance. We highlight injectable hydrogels with various micro-and nanoparticles, because of novelty and attractiveness of this type of materials for CNS regeneration and future development perspectives.

Keywords:

hydrogels, nanoparticles, injectable, microparticles, nanofibers, central nervous system

Affiliations:
Niemczyk B.-IPPT PAN
Sajkiewicz P.Ł.-IPPT PAN
Kołbuk D.-IPPT PAN

Conference abstracts
1.Niemczyk B., Sajkiewicz P., Gradys A., Methylcellulose as a smart thermosensitive scaffold material for tissue engineering, TERMIS EU 2019, TERMIS European Chapter Meeting 2019, Tissue Engineering Therapies: From Concept to Clinical Translation & Commercialisation, 2019-05-27/05-31, Rodos (GR), pp.1435, 2019
2.Niemczyk B., Sajkiewicz P., Gradys A., The Effect of Chemical Composition on Crosslinking Kinetics of Methylcellulose/Agarose Hydrogel, ISBPPB 2018, 4th International Conference on Biomedical Polymers and Polymeric Biomaterials, 2018-07-15/07-18, Kraków (PL), pp.174, 2018
3.Niemczyk B., Gradys A., Sajkiewicz P., The effect of chemical composition on crosslinking kinetics of methylcellulose/agarose hydrogel, PICETE, Polish-Israeli Conference on Electrospinning and Tissue Engineering, 2018-10-04/10-05, Warszawa (PL), pp.25, 2018
4.Niemczyk B., Sajkiewicz P., The effect of chemical composition on viscoelastic properties of methylcellulose/agarose hydrogel, 7th KMM-VIN Industrial Workshop: Biomaterials: Key Technologies for Better Healthcare, 2017-09-27/09-28, Erlangen (DE), pp.44, 2017
Keywords:

methylcellulose, agarose, hydrogel, cross-linking kinetics, DMA, modulus

Affiliations:
Niemczyk B.-IPPT PAN
Sajkiewicz P.-IPPT PAN