Institute of Fundamental Technological Research
Polish Academy of Sciences

Staff

Katarzyna Czarnecka, MSc

Laboratory of Polymers and Biomaterials (SPPiB)
position: doctoral student
telephone: (+48) 22 826 12 81 ext.: 337
room: 335
e-mail:


Recent publications
1.  Czarnecka K., Wojasiński M., Ciach T., Sajkiewicz P., Solution blow spinning of polycaprolactone-rheological determination of spinnability and the effect of processing conditions on fiber diameter and alignment, Materials, ISSN: 1996-1944, DOI: 10.3390/ma14061463, Vol.14, No.6, pp.1463-1-18, 2021

Abstract:
The growing popularity of solution blow spinning as a method for the production of fibrous tissue engineering scaffolds and the vast range of polymer-solvent systems available for the method raises the need to study the effect of processing conditions on fiber morphology and develop a method for its qualitative assessment. Rheological approaches to determine polymer solution spinnability and image analysis approaches to describe fiber diameter and alignment have been previously proposed, although in a separate manner and mostly for the widely known, well-researched electrospinning method. In this study, a series of methods is presented to determine the processing conditions for the development of submicron fibrous scaffolds. Rheological methods are completed with extensive image analysis to determine the spinnability window for a polymer–solvent system and qualitatively establish the influence of polymer solution concentration and collector rotational speed on fiber morphology, diameter, and alignment. Process parameter selection for a tissue engineering scaffold target application is discussed, considering the varying structural properties of the native extracellular matrix of the tissue of interest.

Keywords:
solution blow spinning, rheology, image analysis, nanofibers, fiber alignment, biodegradable nanofibers

Affiliations:
Czarnecka K. - IPPT PAN
Wojasiński M. - Warsaw University of Technology (PL)
Ciach T. - Warsaw University of Technology (PL)
Sajkiewicz P. - IPPT PAN
2.  Woźniak M., Chlanda A., Oberbek P., Heljak M., Czarnecka K., Janeta M., John Ł., Binary bioactive glass composite scaffolds for bone tissue engineering — structure and mechanical properties in micro and nano scale. A preliminary study, Micron, ISSN: 0968-4328, DOI: 10.1016/j.micron.2018.12.006, Vol.119, pp.64-71, 2019

Abstract:
Composite scaffolds of bioactive glass (SiO2-CaO) and bioresorbable polyesters: poly-L-lactic acid (PLLA) and polycaprolactone (PCL) were produced by polymer coating of porous foams. Their structure and mechanical properties were investigated in micro and nanoscale, by the means of scanning electron microscopy, PeakForce Quantitative Nanomechanical Property Mapping (PF-QNM) atomic force microscopy, micro-computed tomography and contact angle measurements. This is one of the first studies in which the nanomechanical properties (elastic modulus, adhesion) were measured and mapped simultaneously with topography imaging (PF-QNM AFM) for bioactive glass and bioactive glass – polymer coated scaffolds. Our findings show that polymer coated scaffolds had higher average roughness and lower stiffness in comparison to pure bioactive glass scaffolds. Such coating-dependent scaffold properties may promote different cells-scaffold interaction.

Keywords:
bone tissue engineering, composite scaffold, bioactive glass, mmechanical properties

Affiliations:
Woźniak M. - Warsaw University of Technology (PL)
Chlanda A. - Warsaw University of Technology (PL)
Oberbek P. - Warsaw University of Technology (PL)
Heljak M. - Warsaw University of Technology (PL)
Czarnecka K. - IPPT PAN
Janeta M. - University of Wrocław (PL)
John Ł. - University of Wrocław (PL)
3.  Chlanda A., Oberbek P., Heljak M., Górecka Ż., Czarnecka K., Chen K.-S., Woźniak M.J., Nanohydroxyapatite adhesion to low temperature plasma modified surface of 3D-printed bone tissue engineering scaffolds - qualitative and quantitative study, SURFACE AND COATINGS TECHNOLOGY, ISSN: 0257-8972, DOI: 10.1016/j.surfcoat.2019.07.070, Vol.375, pp.637-644, 2019

Abstract:
Biodegradable 3D-printed polycaprolactone scaffolds for bone tissue engineering applications have been extensively studied as they can provide an attractive porous architecture mimicking natural bone, with tunable physical and mechanical properties enhancing positive cellular response. The main drawbacks of polycaprolactone-based scaffolds, limiting their applications in tissue engineering are: their hydrophobic nature, low bioactivity and poor mechanical properties compared to native bone tissue. To overcome these issues, the surface of scaffolds is usually modified and covered with a ceramic layer. However, a detailed description of the adhesion forces of ceramic particles to the polymer surface of the scaffolds is still lacking. Our present work is focused on obtaining PCL-based composite scaffolds to strengthen the architecture of the final product. In this manuscript, we report qualitative and quantitative evaluation of low temperature plasma modification followed by detailed studies of the adhesion forces between chemically attached ceramic layer and the surface of polycaprolactone-nanohydroxyapatite composite 3D-printed scaffolds. The results suggest modification-dependent alteration of the internal structure and morphology, as well as mechanical and physical scaffold properties recorded with atomic force microscopy. Moreover, changes in the material surface were followed by enhanced adhesion forces binding the ceramic layer to polymer-based scaffolds.

Keywords:
surface modification, low temperature plasma, atomic force microscopy, bone tissue engineering

Affiliations:
Chlanda A. - Warsaw University of Technology (PL)
Oberbek P. - Warsaw University of Technology (PL)
Heljak M. - Warsaw University of Technology (PL)
Górecka Ż. - Warsaw University of Technology (PL)
Czarnecka K. - IPPT PAN
Chen K.-S. - Tatung University (TW)
Woźniak M.J. - Warsaw University of Technology (PL)
4.  Oberbek P., Kozikowski P., Czarnecka K., Sobiech P., Jakubiak S., Jankowski T., Inhalation exposure to various nanoparticles in work environment — contextual information and results of measurements, Journal of Nanoparticle Research, ISSN: 1388-0764, DOI: 10.1007/s11051-019-4651-x, Vol.21, No.11, pp.222-1-24, 2019

Abstract:
Outside the wide range of potential benefits, the use of nanomaterials can endanger human health, mostly through skin contact and the risk of inhalation. This article presents the results of harmonized measurements with contextual information on the emission of nanoparticles during the manufacturing and application of nanotechnology products. The purpose of the research was to investigate the actual levels of exposure to nano-objects in real working conditions in chosen Polish companies. Measurements were carried out in various workplaces: during silver nanoparticle synthesis, production of thin nanocarbon layers, 3D-printing with the use of a nanohydroxyapatite-polymer composite and the production of special seals from thin glass foils. Research was conducted on the basis of task-based measurements and offline microscopic analysis. Real-time particle DiSCmini counters were used to determine the nano-object concentration during different processes and events: samplers for collecting air dust, and a scanning electron microscope to confirm the presence of nanoparticles emitted from selected sources. Average particle sizes obtained with analysis of microscopic images were as follows: 46.7 ± 13.4 nm, 19.8 ± 4.8 nm, 22.4 ± 7.6 nm, 49.2 ± 26.3 nm respectively for workplaces. The concentration during significant events was referred to the background particle level. During one of the repeated processes, an unexpected and extremely high nanoparticle emission was recorded, which, in the long run, could cause a health hazard to workers. The studies have shown the importance of collective protective measures, revealed unexpected sources of accidentally generated nanoparticles and allowed to obtain knowledge about levels of exposure to nanoparticles during the various processes.

Keywords:
nanoparticles, ultrafine particles, occupational exposure, field monitoring, occupational health, real-time measurement

Affiliations:
Oberbek P. - Warsaw University of Technology (PL)
Kozikowski P. - Central Institute for Labour Protection - National Research Institute, Warsaw (PL)
Czarnecka K. - IPPT PAN
Sobiech P. - Central Institute for Labour Protection - National Research Institute, Warsaw (PL)
Jakubiak S. - Central Institute for Labour Protection - National Research Institute, Warsaw (PL)
Jankowski T. - Central Institute for Labour Protection - National Research Institute, Warsaw (PL)

Patents
Filing No./Date
Filing Publication
Autors
Title
Protection Area, Applicant Name
Patent Number
Date of Grant
pdf
PCT/IB2018/058809
2018-11-09
WO/2019/092641
2019-05-16
Zrodowski L., Ralowicz R. Rozpendowski J., Czarnecka K., Lacisz W., Ostrysz M., Kaczynski K., Tyszko B., Stroz A., Zebrowska B.
Device for manufacturing of spherical metal powders by an ultrasonic atomization method
WO, 3D LAB sp. z o.o.
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424869
2018-03-13
BUP 20/2019
2019-09-23
Łacisz W., Ostrysz M., Czarnecka K., Kaczyński K., Rałowicz R., Żrodowski Ł.
Urządzenie do atomizacji ultradźwiękowej materiałów metalicznych i sposób jego czyszczenia
PL, 3D LAB sp. z o.o.
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424870
2018-03-13
BUP 20/2019
2019-09-23
Żrodowski Ł., Żrodowski C., Wróblewski R., Czarnecka K., Wysocki B., Rałowicz R.
Sonotroda z wewnętrznym systemem chłodzenia
PL, 3D LAB sp. z o.o.
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423442
2017-11-13
BUP 11/2019
2019-05-20
Wysocki B., Żrodowski Ł., Chmielewska A., Święszkowski W., Supeł A., Kurzydłowski K., Czarnecka K.
Sposób wytwarzania addytywnego trójwymiarowych obiektów
PL, Politechnika Warszawska
233190
WUP 09/2019
2019-09-30
423410
2017-11-09
BUP 11/2019
2019-05-20
Żrodowski Ł., Rałowicz R., Rozpendowski J., Czarnecka K.
Urządzenie do wytwarzania sferycznych proszków metali metodą atomizacji ultradźwiękowej
PL, 3D LAB sp. z o.o.
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