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A. Chwojnowski

Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)

Recent publications
1.  Wasyleczko M., Sikorska W., Przytulska M., Dulnik J., Chwojnowski A., Polyester membranes as 3D scaffolds for cell culture, Desalination and Water Treatment, ISSN: 1944-3994, DOI: 10.5004/dwt.2021.26658, Vol.214, pp.181-193, 2021

Abstract:
The study presents two types of three-dimensional membranes made of the biodegradable copolymer. They were obtained by the wet-phase inversion method using different solvent and pore precursors. In one case, a nonwoven made of gelatin and polyvinylpyrrolidone (PVP) as precursors of macropores and small pores, respectively, were used. In the second case, PVP nonwovens and Pluronic were used properly for macro- and micro-pores. As the material, a biodegradable poly(L-lactide-co-ε-caprolactone) is composed of 30% ε-caprolactone and 70% poly(L-lactic acid) was used. Depending on the pore precursors, different membrane structures were obtained. The morphology of pores was studied using the MeMoExplorer™, an advanced software designed for computer analysis of the scanning electron microscopy images. The scaffolds were degraded in phosphate-buffered saline and Hank’s balanced salt solutions at 37°C. Moreover, the porosity of the membranes before and after hydrolysis was calculated.

Keywords:
3D scaffolds, poly(L-lactide-co-ε-caprolactone), porosity of membrane, phase inversion method, degradation of scaffolds

Affiliations:
Wasyleczko M. - other affiliation
Sikorska W. - other affiliation
Przytulska M. - other affiliation
Dulnik J. - IPPT PAN
Chwojnowski A. - Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)
2.  Gadomska‐Gajadhur A., Kruk A., Dulnik J., Chwojnowski A., New polyester biodegradable scaffolds for chondrocyte culturing: preparation, properties, and biological activity, JOURNAL OF APPLIED POLYMER SCIENCE, ISSN: 0021-8995, DOI: 10.1002/app.50089, pp.e50089-1-14, 2020

Abstract:
An innovative modification of the wet inversion phase method, consisting in the use of a polymer nano‐nonwoven as a nonclassic pore precursor. Mechanical properties of the obtained scaffolds were determined, their hydrophilic properties (serum absorbability) were tested, and the content of residues of materials used in the scaffold preparation was determined. Nontoxicity of the developed scaffolds toward T lymphocyte cells was proved. Cultures of primary chondrocytes were obtained successfully. It was proved that an addition of a polymer nano‐nonwoven changes the properties of the scaffolds favorably in respect of their subsequent application in tissue engineering.

Keywords:
cartilage regeneration, chondrocytes, nano-nonwoven, polyvinylpyrrolidone, T lymphocytes

Affiliations:
Gadomska‐Gajadhur A. - other affiliation
Kruk A. - Warsaw University of Technology (PL)
Dulnik J. - IPPT PAN
Chwojnowski A. - Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)
3.  Gadomska‐Gajadhur A., Kruk A., Ruśkowski P., Sajkiewicz P., Dulnik J., Chwojnowski A., Original method of imprinting pores in scaffolds for tissue engineering, Polymers for Advanced Technologies, ISSN: 1042-7147, DOI: 10.1002/pat.5091, pp.1-13, 2020

Abstract:
Results of the preparation of biodegradable porous scaffolds using an original modification of a wet phase inversion method were presented. Influence of gelatin non‐woven as a non‐classic pore precursor and polyvinylpyrrolidone, Pluronic as classic pore precursors on the structure of obtained scaffolds was analyzed. It was shown that the addition of gelatin non‐wovens enables the preparation of scaffolds, which allow for the growth of cells (size, distribution, and shape of pores). Mechanical properties of the obtained cell scaffolds were determined. The influence of pore precursors on mass absorption of scaffolds against isopropanol and plasma was investigated. Interaction of scaffolds with a T‐lymphocyte line (Jurkat) and with fibroblasts (L929) was investigated. Obtained scaffolds are not cytotoxic and can be used as implants, for example, the regeneration of cartilage tissue.

Keywords:
cell cultures, cytotoxic, fibroblasts, imprinted scaffolds

Affiliations:
Gadomska‐Gajadhur A. - other affiliation
Kruk A. - Warsaw University of Technology (PL)
Ruśkowski P. - Warsaw University of Technology (PL)
Sajkiewicz P. - IPPT PAN
Dulnik J. - IPPT PAN
Chwojnowski A. - Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)

Conference papers
1.  Kruk A., Gadomska-Gajadhur A., Ruśkowski P., Chwojnowski A., Dulnik J., Synoradzki L., Preparation of biodegradable semi-permeable membranes as 3D scaffolds for cell cultures, Desalination and Water Treatment, ISSN: 1944-3994, DOI: 10.5004/dwt.2017.11415, Vol.64, pp.317-323, 2017

Abstract:
Results of the preparation of semi-permeable membranes made of biodegradable polymers membranes were presented. Among known polyesters, polylactide was selected for research. The membranes were obtained using wet phase inversion method. The influence of polyvinylpyrrolidone and polymeric nano-non-wovens as pores precursors on the structure of obtained membranes was analysed. It was shown, that utilisation of polymeric nano-non-wovens enabled preparation of semi-permeable membranes, which could be used as wide-pore 3D-type cellular scaffolds.

Keywords:
Biodegradable polymers membranes, Biodegradable polyesters, Porous three-dimensional scaffolds, Inversion phase method

Affiliations:
Kruk A. - Warsaw University of Technology (PL)
Gadomska-Gajadhur A. - Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)
Ruśkowski P. - Warsaw University of Technology (PL)
Chwojnowski A. - Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)
Dulnik J. - IPPT PAN
Synoradzki L. - Warsaw University of Technology (PL)
2.  Chwojnowski A., Kruk A., Wojciechowski C., Łukowska E., Dulnik J., Sajkiewicz P., The dependence of the membrane structure on the non-woven forming the macropores in the 3D scaffolds preparation, Desalination and Water Treatment, ISSN: 1944-3994, DOI: 10.5004/dwt.2017.11394, Vol.64, pp.324-331, 2017

Abstract:
Three types of membrane structures with wide pores were compared in this study. One of the membranes was obtained from polyethersulfone using cellulose fibers as the macropore precursors. Two of the fibers were obtained from poly(L-lactide). As the macropore precursors olyvinylpyrrolidone (1.2 MDa) and pork gelatin non-woven were used, the influence of non-woven fibers on the structure of membranes was shown. Necessity of specific membrane structure application was explained. The hoice of polymers and co-polymers with a range of biodegradation times can determine the scaffold type suitable for the age of a patient.

Keywords:
Polysulfone membrane, Polyester membranes, Membrane structures, Biodegradable membranes, 3D scaffold

Affiliations:
Chwojnowski A. - Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)
Kruk A. - Warsaw University of Technology (PL)
Wojciechowski C. - other affiliation
Łukowska E. - other affiliation
Dulnik J. - IPPT PAN
Sajkiewicz P. - IPPT PAN

Conference abstracts
1.  Kruk A., Gadomska-Gajadhur A., Ruśkowski P., Chwojnowski A., Dulnik J., Synoradzki L., Preparation of biodegradable semi-permeable membranes as 3D scaffolds for cell cultures, MEMPEP 2016, XI-th Conference on Membranes and Membrane Processes in Environmental Protection, 2016-06-15/06-18, Zakopane (PL), pp.24, 2016
2.  Chwojnowski A., Kruk A., Wojciechowski C., Łukowska E., Dulnik J., Sajkiewicz P., The dependence of the membrane structure on the non-woven forming the macropores in the 3D scaffolds preparation, MEMPEP 2016, XI-th Conference on Membranes and Membrane Processes in Environmental Protection, 2016-06-15/06-18, Zakopane (PL), pp.23, 2016

Patents
Filing No./Date
Filing Publication
Autors
Title
Protection Area, Applicant Name
Patent Number
Date of Grant
pdf
414353
2015-10-13
BUP 09/2017
2017-04-24
Chwojnowski A., Łukowska E., Wojciechowski C., Gadomska-Gajadhur A., Kruk A., Ruśkowski P., Synoradzki L., Denis P., Dulnik J., Sajkiewicz P.
Sposób otrzymywania szerokoporowatego, poliestrowego rusztowania komórkowego
PL, Instytut Biocybernetyki i Inżynierii Biomedycznej im. Macieja Nałęcza PAN, Politechnika Warszawska, Instytut Podstawowych Problemów Techniki PAN
228884
WUP 05/2018
2018-05-30



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