Institute of Fundamental Technological Research
Polish Academy of Sciences


Fei-Yau Lu

Recent publications
1.  Wang A., Cheng C., Lin I., Lu F., Tsai H., Lin C., Yang C., Pan P., Kuan C., Chen Y., Lin Y., Chang C., Wu Y., Kurniawan T., Lin C., Wo Andrew M., Chen L., A novel DNA selection and direct extraction process and its application in DNA recombination, Electrophoresis, ISSN: 0173-0835, DOI: 10.1002/elps.201000449, Vol.32, pp.423-430, 2011

In the conventional bench-top approach, the DNA recombination process is time- and effort-consuming due to laborious procedures lasting from several hours to a day. A novel DNA selection and direct extraction process has been proposed, integrated and tested on chip. The integrative microfluidic chip can perform the whole procedure of DNA recombination, including DNA digestion, gel electrophoresis, DNA extraction and insert-vector ligation within 1 h. In this high-throughput design, the manual gel cutting was replaced by an automatic processing system that performed high-quality and high-recovery efficiency in DNA extraction process. With no need of gel-dissolving reagents and manipulation, the application of selection and direct extraction process could significantly eliminate the risks from UV and EtBr and also facilitate DNA recombination. Reliable output with high success rate of cloning has been achieved with a significant reduction in operational hazards, required materials, efforts and time.

Chip integration, DNA recombination, DNA selection and extraction , Microfluidics

Wang A. - National Taiwan University (TW)
Cheng C. - other affiliation
Lin I. - other affiliation
Lu F. - other affiliation
Tsai H. - other affiliation
Lin C. - other affiliation
Yang C. - other affiliation
Pan P. - other affiliation
Kuan C. - other affiliation
Chen Y. - other affiliation
Lin Y. - other affiliation
Chang C. - other affiliation
Wu Y. - other affiliation
Kurniawan T. - other affiliation
Lin C. - other affiliation
Wo Andrew M. - other affiliation
Chen L. - other affiliation

Category A Plus


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