Institute of Fundamental Technological Research
Polish Academy of Sciences

Staff

Angelika Zaszczyńska, MSc

Laboratory of Polymers and Biomaterials (SPPiB)
position: assistant
telephone: (+48) 22 826 12 81 ext.: 171
room: 334
e-mail:

Recent publications
1.  Zaszczyńska A., Moczulska-Heljak M., Gradys A., Sajkiewicz P., Advances in 3D printing for tissue engineering, Materials, ISSN: 1996-1944, DOI: 10.3390/ma14123149, Vol.14, No.12, pp.3149-1-28, 2021

Abstract:
Tissue engineering (TE) scaffolds have enormous significance for the possibility of regeneration of complex tissue structures or even whole organs. Three-dimensional (3D) printing techniques allow fabricating TE scaffolds, having an extremely complex structure, in a repeatable and precise manner. Moreover, they enable the easy application of computer-assisted methods to TE scaffold design. The latest additive manufacturing techniques open up opportunities not otherwise available. This study aimed to summarize the state-of-art field of 3D printing techniques in applications for tissue engineering with a focus on the latest advancements. The following topics are discussed: systematics of the available 3D printing techniques applied for TE scaffold fabrication; overview of 3D printable biomaterials and advancements in 3D-printing-assisted tissue engineering.

Keywords:
tissue engineering, 3D printing, biomaterials

Affiliations:
Zaszczyńska A. - IPPT PAN
Moczulska-Heljak M. - IPPT PAN
Gradys A. - IPPT PAN
Sajkiewicz P. - IPPT PAN
2.  Kaniuk Ł., Ferraris S., Spriano S., Luxbacher T., Krysiak Z., Berniak K., Zaszczyńska A., Marzec M.M., Bernasik A., Sajkiewicz P., Stachewicz U., Time-dependent effects on physicochemical and surface properties of PHBV fibers and films in relation to their interactions with fibroblasts, APPLIED SURFACE SCIENCE, ISSN: 0169-4332, DOI: 10.1016/j.apsusc.2021.148983, Vol.545, pp.148983-1-13, 2021

Abstract:
Biodegradability or materials physicochemical stability are the key biomaterials selection parameters for various medical and tissue engineering applications. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) is a natural copolymer known from its biocompatibility with great support for cells growth and attachment on films and fibers. In our studies, the physicochemical properties of electrospun PHBV fibers and spin-coated films aged for 1, 4 and 8 weeks were analyzed using bulk (FTIR) and surface chemistry (XPS) methods and water contact angle. Further, we characterized the zeta potential changes after aging, by means of electrokinetic measurements, and cell responses to it, using NIH 3T3 murine fibroblasts. Colorimetric MTS cell viability test allowed the assessment of cell proliferation. Additionally, the morphology of fibroblasts and biointerfaces were studied by confocal laser and electron scanning microscopy (CLSM and SEM). These studies indicated that the activity, attachment and proliferation of fibroblasts is independent of aging of PHBV fibers and films. PHBV films show very stable zeta potential over 8 weeks of aging, opposite to PHBV fibers. Importantly, the flat film of PHBV increases cell proliferation, while the fibrous meshes are an excellent support for their stretching. The results of the study revealed clear advantages of PHBV films and fibrous meshes in cell-material interaction.

Keywords:
cell morphology, fibroblast, electrospun fibers, PHBV, Zeta potential

Affiliations:
Kaniuk Ł. - other affiliation
Ferraris S. - other affiliation
Spriano S. - other affiliation
Luxbacher T. - other affiliation
Krysiak Z. - other affiliation
Berniak K. - other affiliation
Zaszczyńska A. - IPPT PAN
Marzec M.M. - other affiliation
Bernasik A. - other affiliation
Sajkiewicz P. - IPPT PAN
Stachewicz U. - AGH University of Science and Technology (PL)
3.  Ghosal K., Augustine R., Zaszczyńska A., Barman M., Jain A., Hasan A., Kalarikkal N., Sajkiewicz P., Thomas S., Novel drug delivery systems based on triaxial electrospinning based nanofibers, REACTIVE AND FUNCTIONAL POLYMERS, ISSN: 1381-5148, DOI: 10.1016/j.reactfunctpolym.2021.104895, Vol.163, pp.104895-1-9, 2021

Abstract:
Electrospinning is a widely investigated process for forming nanofibers. Nanofibers in drug delivery systems are extensively tested due to its remarkable properties e.g. small pore size or large surface area. Recent articles have informed about formation of fibers using triaxial electrospinning in drug delivery systems. This paper summarizes the process of triaxial electrospinning and its application in drug delivery. Triaxial electrospinning has advantages in forming complex nanostructures for specific drug delivery applications. This paper summarizes the possibility to use triaxial electrospinning to resolve the problem of limited drug solubility, to protect biomolecules from hostile environment, and to control drug release kinetics, with the possibility of loading of various drugs. There are literature data evidencing the possibility to achieve sustained release with a border case of zero rate order kinetics. There is no doubt that triaxial electrospinning opens a new way to develop sophisticated nanomaterials for achieving the desired functional performances and to expand the applications in the drug delivery area. Triaxial electrospinning method is interdisciplinary area with great potential in nanotechnology.

Keywords:
triaxial electrospinning, complex nanostructures, drug delivery, desired functional performance, sustained/controlled release

Affiliations:
Ghosal K. - Jadavpur University (IN)
Augustine R. - Qatar University (QA)
Zaszczyńska A. - IPPT PAN
Barman M. - Dr. B. C. Roy College of Pharmacy and Allied Health Sciences (IN)
Jain A. - IPPT PAN
Hasan A. - Qatar University (QA)
Kalarikkal N. - Mahatma Gandhi Central University (IN)
Sajkiewicz P. - IPPT PAN
Thomas S. - Mahatma Gandhi Central University (IN)
4.  Ura D.P., Rosell-Llompart J., Zaszczyńska A., Vasilyev G., Gradys A., Szewczyk P.K., Knapczyk-Korczak J., Avrahami R., Šišková A.O., Arinstein A., Sajkiewicz P., Zussman E., Stachewicz U., The role of electrical polarity in electrospinning and on the mechanical and structural properties of as-spun fibers, Materials, ISSN: 1996-1944, DOI: 10.3390/ma13184169, Vol.13, No.18, pp.4169-1-18, 2020

Abstract:
Electric field strength and polarity in electrospinning processes and their effect on process dynamics and the physical properties of as-spun fibers is studied. Using a solution of the neutral polymer such as poly(methyl methacrylate) (PMMA) we explored the electrospun jet motion issued from a Taylor cone. We focused on the straight jet section up to the incipient stage of the bending instability and on the radius of the disk of the fibers deposited on the collecting electrode. A new correlation formula using dimensionless parameters was found, characterizing the effect of the electric field on the length of the straight jet, L˜E~E˜0.55. This correlation was found to be valid when the spinneret was either negatively or positively charged and the electrode grounded. The fiber deposition radius was found to be independent of the electric field strength and polarity. When the spinneret was negatively charged, L˜E was longer, the as-spun fibers were wider. The positively charged setup resulted in fibers with enhanced mechanical properties and higher crystallinity. This work demonstrates that often-overlooked electrical polarity and field strength parameters influence the dynamics of fiber electrospinning, which is crucial for designing polymer fiber properties and optimizing their collection.

Keywords:
fibers, electrical polarity, charges, electrospinning, PMMA, mechanical properties

Affiliations:
Ura D.P. - AGH University of Science and Technology (PL)
Rosell-Llompart J. - other affiliation
Zaszczyńska A. - IPPT PAN
Vasilyev G. - Technion-Israel Institute of Technology (IL)
Gradys A. - IPPT PAN
Szewczyk P.K. - other affiliation
Knapczyk-Korczak J. - other affiliation
Avrahami R. - other affiliation
Šišková A.O. - other affiliation
Arinstein A. - Technion-Israel Institute of Technology (IL)
Sajkiewicz P. - IPPT PAN
Zussman E. - Technion-Israel Institute of Technology (IL)
Stachewicz U. - AGH University of Science and Technology (PL)
5.  Zaszczyńska A., Gradys A., Sajkiewicz P., Progress in the applications of smart piezoelectric materials for medical devices, Polymers, ISSN: 2073-4360, DOI: 10.3390/polym12112754, Vol.12, No.11, pp.2754-1-19, 2020

Abstract:
Smart piezoelectric materials are of great interest due to their unique properties. Piezoelectric materials can transform mechanical energy into electricity and vice versa. There are mono and polycrystals (piezoceramics), polymers, and composites in the group of piezoelectric materials. Recent years show progress in the applications of piezoelectric materials in biomedical devices due to their biocompatibility and biodegradability. Medical devices such as actuators and sensors, energy harvesting devices, and active scaffolds for neural tissue engineering are continually explored. Sensors and actuators from piezoelectric materials can convert flow rate, pressure, etc., to generate energy or consume it. This paper consists of using smart materials to design medical devices and provide a greater understanding of the piezoelectric effect in the medical industry presently. A greater understanding of piezoelectricity is necessary regarding the future development and industry challenges.

Keywords:
polymers, smart materials, piezoelectric materials, inorganic materials, organic materials, biomedical devices

Affiliations:
Zaszczyńska A. - IPPT PAN
Gradys A. - IPPT PAN
Sajkiewicz P. - IPPT PAN
6.  Zaszczyńska A., Sajkiewicz P.Ł., Gradys A., Tymkiewicz R., Urbanek O., Kołbuk D., Influence of process-material conditions on the structure and biological properties of electrospun polyvinylidene fluoride fibers, BULLETIN OF THE POLISH ACADEMY OF SCIENCES: TECHNICAL SCIENCES, ISSN: 0239-7528, DOI: 10.24425/bpasts.2020.133368, Vol.68, No.3, pp.627-633, 2020

Abstract:
Polyvinylidene fluoride (PVDF) is one of the most important piezoelectric polymers. Piezoelectricity in PVDF appears in polar β and ɣ phases. Piezoelectric fibers obtained by means of electrospinning may be used in tissue engineering (TE) as a smart analogue of the natural extracellular matrix (ECM). We present results showing the effect of rotational speed of the collecting drum on morphology, phase content and in vitro biological properties of PVDF nonwovens. Morphology and phase composition were analyzed using scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (FTIR), respectively. It was shown that increasing rotational speed of the collector leads to an increase in fiber orientation, reduction in fiber diameter and considerable increase of polar phase content, both b and g. In vitro cell culture experiments, carried out with the use of ultrasounds in order to generate electrical potential via piezoelectricity, indicate a positive effect of polar phases on fibroblasts. Our preliminary results demonstrate that piezoelectric PVDF scaffolds are promising materials for tissue engineering applications, particularly for neural tissue regeneration, where the electric potential is crucial.

Keywords:
scaffolds, electrospinning, polyvinylidene fluoride, tissue engineering

Affiliations:
Zaszczyńska A. - IPPT PAN
Sajkiewicz P.Ł. - IPPT PAN
Gradys A. - IPPT PAN
Tymkiewicz R. - IPPT PAN
Urbanek O. - IPPT PAN
Kołbuk D. - IPPT PAN
7.  Zaszczyńska A., Sajkiewicz P., Gradys A., Piezoelectric scaffolds as smart materials for neural tissue engineering, Polymers, ISSN: 2073-4360, DOI: 10.3390/polym12010161, Vol.12, No.1, pp.161-1-25, 2020

Abstract:
Injury to the central or peripheral nervous systems leads to the loss of cognitive and/or sensorimotor capabilities, which still lacks an effective treatment. Tissue engineering in the post-injury brain represents a promising option for cellular replacement and rescue, providing a cell scaffold for either transplanted or resident cells. Tissue engineering relies on scaffolds for supporting cell differentiation and growth with recent emphasis on stimuli responsive scaffolds, sometimes called smart scaffolds. One of the representatives of this material group is piezoelectric scaffolds, being able to generate electrical charges under mechanical stimulation, which creates a real prospect for using such scaffolds in non-invasive therapy of neural tissue. This paper summarizes the recent knowledge on piezoelectric materials used for tissue engineering, especially neural tissue engineering. The most used materials for tissue engineering strategies are reported together with the main achievements, challenges, and future needs for research and actual therapies. This review provides thus a compilation of the most relevant results and strategies and serves as a starting point for novel research pathways in the most relevant and challenging open questions.

Keywords:
neural tissue engineering, piezoelectric scaffolds, smart materials, polymers

Affiliations:
Zaszczyńska A. - IPPT PAN
Sajkiewicz P. - IPPT PAN
Gradys A. - IPPT PAN
8.  Zaszczyńska A., Sajkiewicz P., Gradys A., Kołbuk D., Urbanek O., Cellular studies on piezoelectric polyvinylidene fluoride nanofibers subjected to ultrasounds stimulations, ENGINEERING OF BIOMATERIALS / INŻYNIERIA BIOMATERIAŁÓW, ISSN: 1429-7248, Vol.22, No.153, pp.25-25, 2019
9.  Kecik K., Zaszczyńska A., Mitura A., Experimental Investigations of Energy Recovery from an Electromagnetic Pendulum Vibration Absorber, Journal of Vibration Testing and System Dynamics, ISSN: 2475-4811, DOI: 10.5890/JVTSD.2018.09.002, Vol.2, No.3, pp.209-219, 2018

Abstract:
The paper presents an experimental study of a special non−linear low frequency system dedicated to vibration mitigation and energy recovery. The dual−function design was based on an autoparametric vibration system, which consists of an oscillator with an added pendulum vibration absorber. Its structure includes an energy harvesting device: a levitating magnet in a coil. The pendulum motion shows simultaneously the effects of vibration reduction and energy recovery. The influences of the magnet−coil configurations, and load resistances on vibration reduction and energy harvesting were studied in detail.

Keywords:
Experiment, Energy recovery, Pendulum, Vibration mitigation

Affiliations:
Kecik K. - Lublin University of Technology (PL)
Zaszczyńska A. - other affiliation
Mitura A. - Lublin University of Technology (PL)
10.  Pałka K., Zaszczyńska A., Kleczewska J., Polymerization shrinkage of dental composites, ENGINEERING OF BIOMATERIALS / INŻYNIERIA BIOMATERIAŁÓW, ISSN: 1429-7248, Vol.20, No.143, pp.62, 2017

Abstract:
Dental composites are based on polymer resin matrix which diminishes its volume during polymerization process due to joining of monomer chains [1]. It is the reason of polymerization shrinkage of each polymer material. Serious consequence of the shrinkage in dentistry is marginal leakage and secondary caries resulting from this [2]. Therefore, the develop a low shrinkage material is a big challenge in the manufacturing of dental composites. There are many methods of diminishing polymerization shrinkage. One group is focused on resin matrix composition, the second on filler selection [3] and the others on applying technique [4]. Literature presents a lot of methods of shrinkage measurements [1]. In previous study the Authors used the method based on microCT measurements [5]. In this paper a new approach has been presented. In this study, the new method of polymerization shrinkage was applied to evaluate the polymerization shrinkage of selected dental composites showing differences in composition.

Affiliations:
Pałka K. - Lublin University of Technology (PL)
Zaszczyńska A. - other affiliation
Kleczewska J. - ARKONA laboratory of dental pharmacology (PL)
11.  Pałka K., Zaszczyńska A., Kleczewska J., Polymerization shrinkage of new flow-type dental composite using micro-ct, ENGINEERING OF BIOMATERIALS / INŻYNIERIA BIOMATERIAŁÓW, ISSN: 1429-7248, Vol.19, No.138, pp.75, 2016

Abstract:
Polymerization shrinkage of the resin-based dental composites constitutes a risk of the failure of the interfacial bonds as a result of shrinkage stresses. It may result in marginal leakage, premature failure of the restoration, and even micro-cracking of the tooth [1,2]. Therefore, the research for develop a low shrinkage material has been a goal in the manufacture of dental composites. The color restorative materials are very interesting and market demand for these products was increased recently. They are used especially in milk tooth as fissure sealing, for marking root canal openings or as decoration (tooth tattoo) [3]. In this study, the research of polymerization shrinkage of flow-type dental composites was conducted.

Affiliations:
Pałka K. - Lublin University of Technology (PL)
Zaszczyńska A. - other affiliation
Kleczewska J. - ARKONA laboratory of dental pharmacology (PL)

Conference abstracts
1.  Zaszczyńska A., Sajkiewicz P.Ł., Designing three-dimensional piezoelectric scaffolds for neural tissue engineering, XXII Polish Conference on Biocybernetics and Biomedical Engineering, 2021-05-19/05-21, Warszawa (PL), pp.152, 2021
2.  Zaszczyńska A., Cieciuch A., Gradys A., Lewandowska-Szumieł M., Sajkiewicz P., Cellular studies on stromal cells and piezoelectric nanofibers subjected to ultrasounds stimulations for medical devices, UK-Poland Bioinspired Materials Conference, 2020-11-23/11-24, Lancaster (GB), pp.127-127, 2020
3.  Zaszczyńska A., Sajkiewicz P., Gradys A., Kołbuk D., Urbanek O., Cellular studies of piezoelectric nanofibers with ultrasound stimulations, Aerogels Processing, Modelling and Environmental-Driven Applications, 2019-10-21/10-23, Coimbra (PT), No.P04, pp.36, 2019
4.  Ura D.P., Gradys A., Zaszczyńska A., Sajkiewicz P., Stachewicz U., Controlling of mechanical properties of electrospun PMMA fibers via voltage polarity, 7th Dresden Nanoanalysis Symposium: Nano-scale characterization for cutting-edge materials research and sustainable materials development, 2019-08-30/08-30, Dresden (DE), pp.1-2, 2019
5.  Ura D.P., Gradys A., Zaszczyńska A., Sajkiewicz P., Stachewicz U., Controlling of mechanical properties of electrospun PMMA fibers via voltage polarity, 8th International PhD Meeting, 2019-08-28/08-29, Dresden (DE), pp.1, 2019
6.  Sajkiewicz P., Zaszczyńska A., Piezoelectric scaffolds - on the way to effective cellular mechanotransduction, CNM 2019, 6th CONFERENCE ON NANO- AND MICROMECHANICS, 2019-07-03/07-05, Rzeszów (PL), pp.1, 2019

Keywords:
scaffolds, electro spinning, tissue engineering

Affiliations:
Sajkiewicz P. - IPPT PAN
Zaszczyńska A. - IPPT PAN
7.  Zaszczyńska A., Sajkiewicz P., Gradys A., Urbanek O., Kołbuk D., Influence of process-material conditions on the phase composition, architecture and biological properties of electrospun polyvinylidene fluoride fibers, CNM 2019, 6th CONFERENCE ON NANO- AND MICROMECHANICS, 2019-07-03/07-05, Rzeszów (PL), pp.145-147, 2019

Keywords:
scaffolds, electrospinning, polyvinylidene fluoride, tissue engineering

Affiliations:
Zaszczyńska A. - IPPT PAN
Sajkiewicz P. - IPPT PAN
Gradys A. - IPPT PAN
Urbanek O. - IPPT PAN
Kołbuk D. - IPPT PAN
8.  Ura P.D., Zaszczyńska A., Gradys A., Sajkiewicz P., Stachewicz U., Mechanical properties of electrospun non-woven PMMA mats produced with positive and negative voltage polarities, AMT 2019, XXII Physical Metallurgy and Materials Science Conference: Advanced Materials and Technologies, 2019-06-09/06-12, Bukowina Tatrzańska (PL), pp.1, 2019
9.  Ura D.P., Gradys A., Zaszczyńska A., Sajkiewicz P., Stachewicz U., Effect of voltage polarity on mechanical properties of electrospun PMMA fibers, Frontiers in Polymer Science, 2019-05-05/05-08, Budapest (HU), pp.1-2, 2019
10.  Zaszczyńska A., Sajkiewicz P., Gradys A., Piezoelectric polymeric nanofibers as smart scaffolds for tissue engineering, TERMIS EU 2019, TERMIS European Chapter Meeting 2019, Tissue Engineering Therapies: From Concept to Clinical Translation & Commercialisation, 2019-05-27/05-31, Rodos (GR), pp.1421, 2019
11.  Zaszczyńska A., Pałka K., Polymerization shrinkage of biomaterials, PICETE, Polish-Israeli Conference on Electrospinning and Tissue Engineering, 2018-10-04/10-05, Warszawa (PL), pp.26, 2018
12.  Pałka K., Zaszczyńska A., Kleczewska J., Polymerization shrinkage of dental composites, 26th Annual Conference Biomaterials in Medicine and Veterinary Medicine, 2017-10-12/10-15, Rytro (PL), pp.1, 2017
13.  Pałka K., Zaszczyńska A., Kleczewska J., Polymerization shrinkage of new flow-type dental composite using micro-CT, 25th Annual Conference Biomaterials in Medicine and Veterinary Medicine, 2016-10-13/10-16, Rytro (PL), pp.1, 2016

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