Instytut Podstawowych Problemów Techniki
Polskiej Akademii Nauk

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Ewa Łukowska

Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)

Ostatnie publikacje
1.  Wasyłeczko M., Krysiak Z.J., Łukowska E., Gruba M., Sikorska W., Kruk A., Dulnik J., Czubak J., Chwojnowski A., Three-dimensional scaffolds for bioengineering of cartilage tissue, Biocybernetics and Biomedical Engineering, ISSN: 0208-5216, DOI: 10.1016/j.bbe.2022.03.004, Vol.42, No.2, pp.494-511, 2022

Streszczenie:
The cartilage tissue is neither supplied with blood nor innervated, so it cannot heal by itself. Thus, its reconstruction is highly challenging and requires external support. Cartilage diseases are becoming more common due to the aging population and obesity. Among young people, it is usually a post-traumatic complication. Slight cartilage damage leads to the spontaneous formation of fibrous tissue, not resistant to abrasion and stress, resulting in cartilage degradation and the progression of the disease. For these reasons, cartilage regeneration requires further research, including use of new type of biomaterials for scaffolds. This paper shows cartilage characteristics within its most frequent problems and treatment strategies, including a promising method that combines scaffolds and human cells. Structure and material requirements, manufacturing methods, and commercially available scaffolds were described. Also, the comparison of poly(L-lactide) (PLLA) and polyethersulfone (PES) 3D membranes obtained by a phase inversion method using nonwovens as a pore-forming additives were reported. The scaffolds' structure and the growth ability of human chondrocytes were compared. Scaffolds' structure, cells morphology, and protein presence in the membranes were examined with a scanning electron microscope. The metabolic activity of cells was tested with the MTT assay. The structure of the scaffolds and the growth capacity of human chondrocytes were compared. Obtained results showed higher cell activity and protein content for PES scaffolds than for PLLA. The PES membrane had better mechanical properties (e.g. ripping), greater chondrocytes proliferation, and thus a better secretion of proteins which build up the cartilage structure.

Słowa kluczowe:
3D-scaffolds, membrane structure, polyethersulfone, poly(L-lactide), chondrocyte culture, cartilage regeneration

Afiliacje autorów:
Wasyłeczko M. - Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)
Krysiak Z.J. - inna afiliacja
Łukowska E. - Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)
Gruba M. - Gruca Orthopedic and Trauma Teaching Hospital, Centre of Postgraduate Medical Education (PL)
Sikorska W. - Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)
Kruk A. - Politechnika Warszawska (PL)
Dulnik J. - IPPT PAN
Czubak J. - Gruca Orthopedic and Trauma Teaching Hospital, Centre of Postgraduate Medical Education (PL)
Chwojnowski A. - Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)
140p.
2.  Chwojnowski A., Wojciechowski C., Lewińska D., Łukowska E., Nowak J., Kupikowska-Stobba B., Grzeczkowicz M., Studies on the structure of semipermeable membranes by means of SEM. Problems and potential sources of errors, Biocybernetics and Biomedical Engineering, ISSN: 0208-5216, DOI: 10.1016/s0208-5216(12)70032-3, Vol.32, No.1, pp.51-64, 2012

Prace konferencyjne
1.  Chwojnowski A., Kruk A., Wojciechowski C., Łukowska E., Dulnik J., Sajkiewicz P., The dependence of the membrane structure on the non-woven forming the macropores in the 3D scaffolds preparation, Desalination and Water Treatment, ISSN: 1944-3994, DOI: 10.5004/dwt.2017.11394, Vol.64, pp.324-331, 2017

Streszczenie:
Three types of membrane structures with wide pores were compared in this study. One of the membranes was obtained from polyethersulfone using cellulose fibers as the macropore precursors. Two of the fibers were obtained from poly(L-lactide). As the macropore precursors olyvinylpyrrolidone (1.2 MDa) and pork gelatin non-woven were used, the influence of non-woven fibers on the structure of membranes was shown. Necessity of specific membrane structure application was explained. The hoice of polymers and co-polymers with a range of biodegradation times can determine the scaffold type suitable for the age of a patient.

Słowa kluczowe:
Polysulfone membrane, Polyester membranes, Membrane structures, Biodegradable membranes, 3D scaffold

Afiliacje autorów:
Chwojnowski A. - Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)
Kruk A. - Politechnika Warszawska (PL)
Wojciechowski C. - inna afiliacja
Łukowska E. - Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)
Dulnik J. - IPPT PAN
Sajkiewicz P. - IPPT PAN
20p.

Abstrakty konferencyjne
1.  Chwojnowski A., Kruk A., Wojciechowski C., Łukowska E., Dulnik J., Sajkiewicz P., The dependence of the membrane structure on the non-woven forming the macropores in the 3D scaffolds preparation, MEMPEP 2016, XI-th Conference on Membranes and Membrane Processes in Environmental Protection, 2016-06-15/06-18, Zakopane (PL), pp.23, 2016

Patenty
Numer/data zgłoszenia patentowego
Ogłoszenie o zgłoszeniu patentowym
Twórcy
Tytuł
Kraj i Nazwa uprawnionego z patentu
Numer patentu
Ogłoszenie o udzieleniu patentu
pdf
414353
2015-10-13
BUP 09/2017
2017-04-24
Chwojnowski A., Łukowska E., Wojciechowski C., Gadomska-Gajadhur A., Kruk A., Ruśkowski P., Synoradzki L., Denis P., Dulnik J., Sajkiewicz P.
Sposób otrzymywania szerokoporowatego, poliestrowego rusztowania komórkowego
PL, Instytut Biocybernetyki i Inżynierii Biomedycznej im. Macieja Nałęcza PAN, Politechnika Warszawska, Instytut Podstawowych Problemów Techniki PAN
228884
WUP 05/2018
2018-05-30



402004
2012-12-11
BUP 13/2014
2014-06-23
Lewińska D., Kupikowska-Stobba B., Chwojnowski A., Grzeczkowicz M., Łukowska E.
Sposób oznaczania stężenia komórek
PL, Instytut Biocybernetyki i Inżynierii Biomedycznej PAN
223717
WUP 10/2016
2016-10-31



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