Instytut Podstawowych Problemów Techniki
Polskiej Akademii Nauk


Julien Aureille

Ostatnie publikacje
1.  Błoński S., Aureille J., Badawi S., Zaremba D., Pernet L., Grichine A., Fraboulet S., Korczyk P.M., Recho P., Guilluy Ch., Dolega M.E., Direction of epithelial folding defines impact of mechanical forces on epithelial state, Developmental Cell, ISSN: 1534-5807, DOI: 10.1016/j.devcel.2021.11.008, Vol.56, pp.3222-3234, 2021

Cell shape dynamics during development is tightly regulated and coordinated with cell fate determination. Triggered by an interplay between biochemical and mechanical signals, epithelia form complex tissues by undergoing coordinated cell shape changes, but how such spatiotemporal coordination is controlled remains an open question. To dissect biochemical signaling from purely mechanical cues, we developed a microfluidic system that experimentally triggers epithelial folding to recapitulate stereotypic deformations observed in vivo. Using this system, we observe that the apical or basal direction of folding results in strikingly different mechanical states at the fold boundary, where the balance between tissue tension and torque (arising from the imposed curvature) controls the spread of folding-induced calcium waves at a short timescale and induces spatial patterns of gene expression at longer timescales. Our work uncovers that folding-associated gradients of cell shape and their resulting mechanical stresses direct spatially distinct biochemical responses within the monolayer.

Słowa kluczowe:
epithelial morphogenesis, epithelial folding, tension, calcium waves, microfluidics, RNAseq

Afiliacje autorów:
Błoński S. - IPPT PAN
Aureille J. - inna afiliacja
Badawi S. - inna afiliacja
Zaremba D. - IPPT PAN
Pernet L. - inna afiliacja
Grichine A. - inna afiliacja
Fraboulet S. - inna afiliacja
Korczyk P.M. - IPPT PAN
Recho P. - inna afiliacja
Guilluy Ch. - inna afiliacja
Dolega M.E. - Université Grenoble Alpes (FR)

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