Instytut Podstawowych Problemów Techniki
Polskiej Akademii Nauk


N. Frerker

Ostatnie publikacje
1.  Kosik-Kozioł A., Costantini M., Mróz A., Idaszek J., Heljak M., Jaroszewicz J., Kijeńska E., Szöke K., Frerker N., Barbetta A., Brinchmann J.E., Święszkowski W., 3D bioprinted hydrogel model incorporating β-tricalcium phosphate for calcified cartilage tissue engineering, Biofabrication, ISSN: 1758-5082, DOI: 10.1088/1758-5090/ab15cb, Vol.11, No.3, pp.035016-1-29, 2019

One promising strategy to reconstruct osteochondral defects relies on 3D bioprinted three-zonal structures comprised of hyaline cartilage, calcified cartilage, and subchondral bone. So far, several studies have pursued the regeneration of either hyaline cartilage or bone in vitro while—despite its key role in the osteochondral region—only few of them have targeted the calcified layer. In this work, we present a 3D biomimetic hydrogel scaffold containing β-tricalcium phosphate (TCP) for engineering calcified cartilage through a co-axial needle system implemented in extrusion-based bioprinting process. After a thorough bioink optimization, we showed that 0.5% w/v TCP is the optimal concentration forming stable scaffolds with high shape fidelity and endowed with biological properties relevant for the development of calcified cartilage. In particular, we investigate the effect induced by ceramic nano-particles over the differentiation capacity of bioprinted bone marrow-derived human mesenchymal stem cells in hydrogel scaffolds cultured up to 21 d in chondrogenic media. To confirm the potential of the presented approach to generate a functional in vitro model of calcified cartilage tissue, we evaluated quantitatively gene expression of relevant chondrogenic (COL1, COL2, COL10A1, ACAN) and osteogenic (ALPL, BGLAP) gene markers by means of RT-qPCR and qualitatively by means of fluorescence immunocytochemistry.

Słowa kluczowe:
alginate, gelatin methacrylate, ß-tricalcium phosphate TCP, bioprinting, coaxial needle, calcified cartilage

Afiliacje autorów:
Kosik-Kozioł A. - inna afiliacja
Costantini M. - Sapienza University of Rome (IT)
Mróz A. - inna afiliacja
Idaszek J. - inna afiliacja
Heljak M. - Politechnika Warszawska (PL)
Jaroszewicz J. - inna afiliacja
Kijeńska E. - inna afiliacja
Szöke K. - inna afiliacja
Frerker N. - inna afiliacja
Barbetta A. - Sapienza University of Rome (IT)
Brinchmann J.E. - inna afiliacja
Święszkowski W. - inna afiliacja

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