Instytut Podstawowych Problemów Techniki
Polskiej Akademii Nauk

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Z. Krysiak


Ostatnie publikacje
1.  Kaniuk Ł., Ferraris S., Spriano S., Luxbacher T., Krysiak Z., Berniak K., Zaszczyńska A., Marzec M.M., Bernasik A., Sajkiewicz P., Stachewicz U., Time-dependent effects on physicochemical and surface properties of PHBV fibers and films in relation to their interactions with fibroblasts, APPLIED SURFACE SCIENCE, ISSN: 0169-4332, DOI: 10.1016/j.apsusc.2021.148983, Vol.545, pp.148983-1-13, 2021

Streszczenie:
Biodegradability or materials physicochemical stability are the key biomaterials selection parameters for various medical and tissue engineering applications. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) is a natural copolymer known from its biocompatibility with great support for cells growth and attachment on films and fibers. In our studies, the physicochemical properties of electrospun PHBV fibers and spin-coated films aged for 1, 4 and 8 weeks were analyzed using bulk (FTIR) and surface chemistry (XPS) methods and water contact angle. Further, we characterized the zeta potential changes after aging, by means of electrokinetic measurements, and cell responses to it, using NIH 3T3 murine fibroblasts. Colorimetric MTS cell viability test allowed the assessment of cell proliferation. Additionally, the morphology of fibroblasts and biointerfaces were studied by confocal laser and electron scanning microscopy (CLSM and SEM). These studies indicated that the activity, attachment and proliferation of fibroblasts is independent of aging of PHBV fibers and films. PHBV films show very stable zeta potential over 8 weeks of aging, opposite to PHBV fibers. Importantly, the flat film of PHBV increases cell proliferation, while the fibrous meshes are an excellent support for their stretching. The results of the study revealed clear advantages of PHBV films and fibrous meshes in cell-material interaction.

Słowa kluczowe:
cell morphology, fibroblast, electrospun fibers, PHBV, Zeta potential

Afiliacje autorów:
Kaniuk Ł. - inna afiliacja
Ferraris S. - inna afiliacja
Spriano S. - inna afiliacja
Luxbacher T. - inna afiliacja
Krysiak Z. - inna afiliacja
Berniak K. - inna afiliacja
Zaszczyńska A. - IPPT PAN
Marzec M.M. - inna afiliacja
Bernasik A. - inna afiliacja
Sajkiewicz P. - IPPT PAN
Stachewicz U. - AGH University of Science and Technology (PL)
140p.

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