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Rybak D., Rinoldi C., Nakielski P., Du J.♦, Haghighat Bayan Mohammad A., Zargarian Seyed S., Pruchniewski M.♦, Li X.♦, Strojny-Cieślak B.♦, Ding B.♦, Pierini F., Injectable and self-healable nano-architectured hydrogel for NIR-light responsive chemo- and photothermal bacterial eradication,
JOURNAL OF MATERIALS CHEMISTRY B , ISSN: 2050-7518, DOI: 10.1039/D3TB02693K, pp.1-21, 2024Abstract: Hydrogels with multifunctional properties activated at specific times have gained significant attention in the biomedical field. As bacterial infections can cause severe complications that negatively impact wound repair, herein, we present the development of a stimuli-responsive, injectable, and in situ-forming hydrogel with antibacterial, self-healing, and drug-delivery properties. In this study, we prepared a Pluronic F-127 (PF127) and sodium alginate (SA)-based hydrogel that can be targeted to a specific tissue via injection. The PF127/SA hydrogel was incorporated with polymeric short-filaments (SFs) containing an anti-inflammatory drug – ketoprofen, and stimuli-responsive polydopamine (PDA) particles. The hydrogel, after injection, could be in situ gelated at the body temperature, showing great in vitro stability and self-healing ability after 4 h of incubation. The SFs and PDA improved the hydrogel injectability and compressive strength. The introduction of PDA significantly accelerated the KET release under near-infrared light exposure and extended its release validity period. The excellent composites’ photo-thermal performance led to antibacterial activity against representative Gram-positive and Gram-negative bacteria, resulting in 99.9% E. coli and S. aureus eradication after 10 min of NIR light irradiation. In vitro, fibroblast L929 cell studies confirmed the materials’ biocompatibility and paved the way toward further in vivo and clinical application of the system for chronic wound treatments. Affiliations:
Rybak D. | - | IPPT PAN | Rinoldi C. | - | IPPT PAN | Nakielski P. | - | IPPT PAN | Du J. | - | University of California (US) | Haghighat Bayan Mohammad A. | - | IPPT PAN | Zargarian Seyed S. | - | IPPT PAN | Pruchniewski M. | - | other affiliation | Li X. | - | Donghua University (CN) | Strojny-Cieślak B. | - | other affiliation | Ding B. | - | Donghua University (CN) | Pierini F. | - | IPPT PAN |
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Haghighat Bayan Mohammad A., Rinoldi C., Rybak D., Zargarian Seyed S., Zakrzewska A., Cegielska O., Põhako-Palu K.♦, Zhang S.♦, Stobnicka-Kupiec A.♦, Górny Rafał L.♦, Nakielski P., Kogermann K.♦, De Sio L.♦, Ding B.♦, Pierini F., Engineering surgical face masks with photothermal and photodynamic plasmonic nanostructures for enhancing filtration and on-demand pathogen eradication,
Biomaterials Science, ISSN: 2047-4849, DOI: 10.1039/d3bm01125a, pp.1-15, 2024Abstract: The shortage of face masks and the lack of antipathogenic functions has been significant since the recent pandemic's inception. Moreover, the disposal of an enormous number of contaminated face masks not only carries a significant environmental impact but also escalates the risk of cross-contamination. This study proposes a strategy to upgrade available surgical masks into antibacterial masks with enhanced particle and bacterial filtration. Plasmonic nanoparticles can provide photodynamic and photothermal functionalities for surgical masks. For this purpose, gold nanorods act as on-demand agents to eliminate pathogens on the surface of the masks upon near-infrared light irradiation. Additionally, the modified masks are furnished with polymer electrospun nanofibrous layers. These electrospun layers can enhance the particle and bacterial filtration efficiency, not at the cost of the pressure drop of the mask. Consequently, fabricating these prototype masks could be a practical approach to upgrading the available masks to alleviate the environmental toll of disposable face masks. Affiliations:
Haghighat Bayan Mohammad A. | - | IPPT PAN | Rinoldi C. | - | IPPT PAN | Rybak D. | - | IPPT PAN | Zargarian Seyed S. | - | IPPT PAN | Zakrzewska A. | - | IPPT PAN | Cegielska O. | - | IPPT PAN | Põhako-Palu K. | - | other affiliation | Zhang S. | - | other affiliation | Stobnicka-Kupiec A. | - | other affiliation | Górny Rafał L. | - | other affiliation | Nakielski P. | - | IPPT PAN | Kogermann K. | - | other affiliation | De Sio L. | - | Sapienza University of Rome (IT) | Ding B. | - | Donghua University (CN) | Pierini F. | - | IPPT PAN |
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Nakielski P., Rybak D., Jezierska-Woźniak K.♦, Rinoldi C., Sinderewicz E.♦, Staszkiewicz-Chodor J.♦, Haghighat Bayan Mohammad A., Czelejewska W.♦, Urbanek-Świderska O., Kosik-Kozioł A., Barczewska M.♦, Skomorowski M.♦, Holak P.♦, Lipiński S.♦, Maksymowicz W.♦, Pierini F., Minimally invasive intradiscal delivery of BM-MSCs via fibrous microscaffold carriers,
ACS Applied Materials and Interfaces, ISSN: 1944-8244, DOI: 10.1021/acsami.3c11710, pp.1-16, 2023Abstract: Current treatments of degenerated intervertebral discs often provide only temporary relief or address specific causes, necessitating the exploration of alternative therapies. Cell-based regenerative approaches showed promise in many clinical trials, but
limitations such as cell death during injection and a harsh disk environment hinder their effectiveness. Injectable microscaffolds offer a solution by providing a supportive microenvironment for cell delivery and enhancing bioactivity. This study evaluated the
safety and feasibility of electrospun nanofibrous microscaffolds modified with chitosan (CH) and chondroitin sulfate (CS) for treating degenerated NP tissue in a large animal model. The microscaffolds facilitated cell attachment and acted as an effective delivery system, preventing cell leakage under a high disc pressure. Combining microscaffolds with bone marrow-derived mesenchymal stromal cells demonstrated no cytotoxic effects and proliferation over the entire microscaffolds. The administration of cells attached to microscaffolds into the NP positively influenced the regeneration process of the intervertebral disc. Injectable poly(L-lactide-co-glycolide) and poly(L-lactide) microscaffolds enriched with CH or CS, having a fibrous structure, showed the potential to promote intervertebral disc regeneration. These features collectively address critical challenges in the fields of tissue engineering and regenerative medicine, particularly in the context of intervertebral disc degeneration. Keywords: microscaffolds,cell carriers,injectable biomaterials,intervertebral disc,laser micromachining,electrospinning Affiliations:
Nakielski P. | - | IPPT PAN | Rybak D. | - | IPPT PAN | Jezierska-Woźniak K. | - | other affiliation | Rinoldi C. | - | IPPT PAN | Sinderewicz E. | - | other affiliation | Staszkiewicz-Chodor J. | - | other affiliation | Haghighat Bayan Mohammad A. | - | IPPT PAN | Czelejewska W. | - | other affiliation | Urbanek-Świderska O. | - | IPPT PAN | Kosik-Kozioł A. | - | IPPT PAN | Barczewska M. | - | University of Warmia and Mazury in Olsztyn (PL) | Skomorowski M. | - | other affiliation | Holak P. | - | other affiliation | Lipiński S. | - | other affiliation | Maksymowicz W. | - | University of Warmia and Mazury in Olsztyn (PL) | Pierini F. | - | IPPT PAN |
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Rybak D., Su Y.♦, Li Y.♦, Ding B.♦, Lv X.♦, Li Z.♦, Yeh Y.♦, Nakielski P., Rinoldi C., Pierini F., Dodda Jagan M.♦, Evolution of nanostructured skin patches towards multifunctional wearable platforms for biomedical applications,
NANOSCALE, ISSN: 2040-3364, DOI: 10.1039/D3NR00807J, Vol.15, No.18, pp.8044-8083, 2023Abstract: Recent advances in the field of skin patches have promoted the development of wearable and implantable bioelectronics for long-term, continuous healthcare management and targeted therapy. However, the design of electronic skin (e-skin) patches with stretchable components is still challenging and requires an in-depth understanding of the skin-attachable substrate layer, functional biomaterials and advanced self-powered electronics. In this comprehensive review, we present the evolution of skin patches from functional nanostructured materials to multi-functional and stimuli-responsive patches towards flexible substrates and emerging biomaterials for e-skin patches, including the material selection, structure design and promising applications. Stretchable sensors and self-powered e-skin patches are also discussed, ranging from electrical stimulation for clinical procedures to continuous health monitoring and integrated systems for comprehensive healthcare management. Moreover, an integrated energy harvester with bioelectronics enables the fabrication of self-powered electronic skin patches, which can effectively solve the energy supply and overcome the drawbacks induced by bulky battery-driven devices. However, to realize the full potential offered by these advancements, several challenges must be addressed for next-generation e-skin patches. Finally, future opportunities and positive outlooks are presented on the future directions of bioelectronics. It is believed that innovative material design, structure engineering, and in-depth study of fundamental principles can foster the rapid evolution of electronic skin patches, and eventually enable self-powered close-looped bioelectronic systems to benefit mankind. Affiliations:
Rybak D. | - | IPPT PAN | Su Y. | - | other affiliation | Li Y. | - | other affiliation | Ding B. | - | Donghua University (CN) | Lv X. | - | other affiliation | Li Z. | - | other affiliation | Yeh Y. | - | other affiliation | Nakielski P. | - | IPPT PAN | Rinoldi C. | - | IPPT PAN | Pierini F. | - | IPPT PAN | Dodda Jagan M. | - | other affiliation |
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Haghighat Bayan Mohammad A., Dias Yasmin J.♦, Rinoldi C., Nakielski P., Rybak D., Truong Yen B.♦, Yarin A.♦, Pierini F., Near-infrared light activated core-shell electrospun nanofibers decorated with photoactive plasmonic nanoparticles for on-demand smart drug delivery applications,
Journal of Polymer Science, ISSN: 2642-4169, DOI: 10.1002/pol.20220747, Vol.61, No.7, pp.521-533, 2023Abstract: Over the last few years, traditional drug delivery systems (DDSs) have been transformed into smart DDSs. Recent advancements in biomedical nanotech-nology resulted in introducing stimuli-responsiveness to drug vehicles. Nano-
platforms can enhance drug release efficacy while reducing the side effects of drugs by taking advantage of the responses to specific internal or external stim-uli. In this study, we developed an electrospun nanofibrous photo-responsive DDSs. The photo-responsivity of the platform enables on-demand elevated drug release. Furthermore, it can provide a sustained release profile and pre-vent burst release and high concentrations of drugs. A coaxial electrospinning setup paired with an electrospraying technique is used to fabricate core-shell PVA-PLGA nanofibers decorated with plasmonic nanoparticles. The fabricated
nanofibers have a hydrophilic PVA and Rhodamine-B (RhB) core, while the shell is hydrophobic PLGA decorated with gold nanorods (Au NRs). The presence of plasmonic nanoparticles enables the platform to twice the amount of drug release besides exhibiting a long-term release. Investigations into the photo-responsive release mechanism demonstrate the system's potential as a “smart” drug delivery platform. Keywords: electrospun core-shell nanofibers,NIR-light activation,on-demand drug release,plasmonic nanoparticles,stimuli-responsive nanomaterials Affiliations:
Haghighat Bayan Mohammad A. | - | IPPT PAN | Dias Yasmin J. | - | other affiliation | Rinoldi C. | - | IPPT PAN | Nakielski P. | - | IPPT PAN | Rybak D. | - | IPPT PAN | Truong Yen B. | - | other affiliation | Yarin A. | - | Technion-Israel Institute of Technology (IL) | Pierini F. | - | IPPT PAN |
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Nakielski P., Rinoldi C., Pruchniewski M.♦, Pawłowska S., Gazińska M.♦, Strojny B.♦, Rybak D., Jezierska-Woźniak K.♦, Urbanek O., Denis P., Sinderewicz E.♦, Czelejewska W.♦, Staszkiewicz-Chodor J.♦, Grodzik M.♦, Ziai Y., Barczewska M.♦, Maksymowicz W.♦, Pierini F., Laser-assisted fabrication of injectable nanofibrous cell carriers,
Small, ISSN: 1613-6810, DOI: 10.1002/smll.202104971, Vol.18, No.2, pp.2104971-1-18, 2022Abstract: The use of injectable biomaterials for cell delivery is a rapidly expanding field which may revolutionize the medical treatments by making them less invasive. However, creating desirable cell carriers poses significant challenges to the clinical implementation of cell-based therapeutics. At the same time, no method has been developed to produce injectable microscaffolds (MSs) from electrospun materials. Here the fabrication of injectable electrospun nanofibers is reported on, which retain their fibrous structure to mimic the extracellular matrix. The laser-assisted micro-scaffold fabrication has produced tens of thousands of MSs in a short time. An efficient attachment of cells to the surface and their proliferation is observed, creating cell-populated MSs. The cytocompatibility assays proved their biocompatibility, safety, and potential as cell carriers. Ex vivo results with the use of bone and cartilage tissues proved that NaOH hydrolyzed and chitosan functionalized MSs are compatible with living tissues and readily populated with cells. Injectability studies of MSs showed a high injectability rate, while at the same time, the force needed to eject the load is no higher than 25 N. In the future, the produced MSs may be studied more in-depth as cell carriers in minimally invasive cell therapies and 3D bioprinting applications. Affiliations:
Nakielski P. | - | IPPT PAN | Rinoldi C. | - | IPPT PAN | Pruchniewski M. | - | other affiliation | Pawłowska S. | - | IPPT PAN | Gazińska M. | - | other affiliation | Strojny B. | - | other affiliation | Rybak D. | - | IPPT PAN | Jezierska-Woźniak K. | - | other affiliation | Urbanek O. | - | IPPT PAN | Denis P. | - | IPPT PAN | Sinderewicz E. | - | other affiliation | Czelejewska W. | - | other affiliation | Staszkiewicz-Chodor J. | - | other affiliation | Grodzik M. | - | other affiliation | Ziai Y. | - | IPPT PAN | Barczewska M. | - | University of Warmia and Mazury in Olsztyn (PL) | Maksymowicz W. | - | University of Warmia and Mazury in Olsztyn (PL) | Pierini F. | - | IPPT PAN |
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