Piotr Denis, M.Sc., Eng.

Laboratory of Polymers and Biomaterials (SPPiB)
position: specialist
telephone: (+48) 22 826 12 81 ext.: 211
room: 343
e-mail: pdenis

Recent publications
1.Dulnik J., Denis P., Sajkiewicz P., Kołbuk D., Choińska E., Biodegradation of bicomponent PCL/gelatin and PCL/collagen nanofibers electrospun from alternative solvent system, Polymer Degradation and Stability, ISSN: 0141-3910, DOI: 10.1016/j.polymdegradstab.2016.05.022, Vol.130, pp.10-21, 2016
Abstract:

Bicomponent polycaprolactone/gelatin and polycaprolactone/collagen nanofibers formed by electrospinning using various solvents were subjected to biodegradation and compared. Hexafluoroisopropanol (HFIP) was used as a reference solvent, while the second, alternative solvent system was the mixture of acetic acid (AA) with formic acid (FA). Biodegradation of investigated materials was manifested mainly by the gelatin leaching, including collagen which is indeed denaturated to gelatin during electrospinning, leading to nanofibers erosion. There was no molecular degradation of PCL during 90 days of biodegradation procedure as deduced from no change in the elongation stress at break. The rate of biopolymer leaching was very fast from all materials during the first 24 h of biodegradation, being related to surface leaching, followed by a slower rate leaching from deeper material layers. Mass measurements showed much faster biopolymer leaching from nanofibers electrospun from AA/FA than from HFIP because of strongly emulsive nature of the solution in the former case. Irrespective of the solvent used, the leaching rate increased with initial content of gelatin. The analysis of Young modulus during biodegradation indicated complex mechanism of changes, including biopolymer mass loss, increase of PCL crystallinity and partial gelatin renaturation.

Keywords:

Bicomponent nanofibers, Biodegradation, Biopolymer

Affiliations:
Dulnik J.-IPPT PAN
Denis P.-IPPT PAN
Sajkiewicz P.-IPPT PAN
Kołbuk D.-IPPT PAN
Choińska E.-Warsaw University of Technology (PL)
2.Chrzanowska J., Hoffman J., Denis P., Giżyński M., Mościcki T., The effect of process parameters on rhenium diboride films deposited by PLD, SURFACE AND COATINGS TECHNOLOGY, ISSN: 0257-8972, DOI: 10.1016/j.surfcoat.2015.07.024, Vol.277, pp.15-22, 2015
Abstract:

Rhenium diboride (ReB2) thin films have been deposited by a nanosecond pulsed laser deposition method on Si (100) substrate heated to 570°C. The coatings were formed in the ablation process of SPS sintered ReB2 target. The effect of laser wavelength, energy density and postannealing on the films' properties was studied. Investigated wavelengths were 355 and 1064 nm of Nd:YAG nanosecond laser. Laser beam energy density varied from 2.1 to 6.1 J cm− 2 and from 4.1 to 9.4 J cm− 2 at 355 and 1064 nm, respectively. Layer thickness was of the order of several hundred nanometres. Deposition efficiency increases with an energy density in quasi linear way and strongly grows for shorter wavelength. The layers consist of two characteristic microstructures: a smooth basis and stick debris (typical diameters of several hundred nanometres). Lower energy density of laser beam and longer wavelength favour dominance of smooth basis and minimization of debris. The XRD analysis of all samples indicate the crystalline ReB2 with preferred (002) orientation and fine grain size of about 20 nm. Shorter wavelength and higher energy density foster stronger (002) orientation. Moreover, an annealing right after the deposition (25 min in 350°C) causes minimization of degree of orientation and decrease of hardness. The Vickers hardness of ReB2 films is at about 60 GPa and is reduced to about 40 GPa after the annealing process. Deposition efficiency and physical and chemical structures of layers produced under variety of conditions were studied and compared.

Keywords:

Ceramics ablation, Pulsed laser deposition, Rhenium diboride, ReB2 optical properties, Super-hard films, Wavelength influence

Affiliations:
Chrzanowska J.-IPPT PAN
Hoffman J.-IPPT PAN
Denis P.-IPPT PAN
Giżyński M.-other affiliation
Mościcki T.-IPPT PAN
3.Denis P., Dulnik J., Sajkiewicz P., Electrospinning and Structure of Bicomponent Polycaprolactone/Gelatin Nanofibers Obtained Using Alternative Solvent System, International Journal of Polymeric Materials and Polymeric Biomaterials, ISSN: 0091-4037, DOI: 10.1080/00914037.2014.945208, Vol.64, No.7, pp.354-364, 2015
Abstract:

Bicomponent polycaprolactone/gelatin (PCL/Gt) nanofibers were successfully formed for the first time by electrospinning using a novel polymer–solvent system with solvents being alternative to the commonly used toxic solvents like fluorinated alcohols. The mixture of acetic acid (AA) with formic acid (FA; 90:10) was applied. Stable electrospinning was possible despite the fact the mixture of PCL and gelatin in AA/FA solvent showed emulsive structure. From the practical perspective, there is no doubt that it is possible to obtain PCL/Gt fibers using AA/FA mixture with morphology similar to that for fibers spun from hexafluoroisopropanol (HFIP) solutions.

Keywords:

Alternative solvents, electrospinning, gelatin, nanofibers, polycaprolactone, structure

Affiliations:
Denis P.-IPPT PAN
Dulnik J.-IPPT PAN
Sajkiewicz P.-IPPT PAN
4.Sajkiewicz P., Brzeska J., Denis P., Sikorska W., Kowalczuk M., Rutkowska M., The preliminary studies of a structure and electrospinning of new polyurethanes based on synthetic atactic poly[(R, S)-3-hydroxybutyrate], BULLETIN OF THE POLISH ACADEMY OF SCIENCES: TECHNICAL SCIENCES, ISSN: 0239-7528, DOI: 10.2478/bpasts-2014-0006, Vol.62, No.1, pp.55-60, 2014
Abstract:

Novel polyurethanes based on synthetic, atactic poly[(R, S)-3-hydroxybutyrate] (a-PHB) and polycaprolactone (PCL) or polyoxytetramethylene (PTMG) diols were synthesized. It was shown that the presence of a-PHB within soft segments reduces crystallinity of PUR. Because of the low melting temperature for polyurethanes with PCL in soft segments, at this stage of work, electrospinning was limited to polyurethanes containing PTMG and a-PHB. Polyurethane containing 80% of PTMG and 20% of a-PHB was electrospun at various parameters from hexafluoro-2-propanole solution, resulting in formation of fibers with the average diameter ca. 2 μm. The fiber diameter decreased with decreasing polymer concentration in a solution and was practically insensitive to the needle-collector distance in the applied range of distances.

Keywords:

polyurethane, polyhydroxybutyrate, electrospinning, scaffolds

Affiliations:
Sajkiewicz P.-IPPT PAN
Brzeska J.-other affiliation
Denis P.-IPPT PAN
Sikorska W.-other affiliation
Kowalczuk M.-other affiliation
Rutkowska M.-other affiliation
5.Jóźwiak-Niedźwiedzka D., Brandt A.M., Gibas K., Denis P., The alkali - aggregate reaction hazard in the case of barite concretes, CEMENT, WAPNO, BETON, ISSN: 1425-8129, Vol.19, No.4, pp.234-242, 2014
Abstract:

Concretes for the shields against ionizing radiation should be executed with particular care to satisfy all requirements, not only effective attenuation of different radiation but also sufficient durability. In the paper fundamental problems are presented that are related with designing of such protective concretes in order to avoid alkali-aggregate reaction. The results of preliminary investigations are presented that indicate how to select barite aggregate and to define composition of concrete mix. Present knowledge in that area of materials engineering should be completed before initiation of construction of nuclear power stations at industrial scale in Poland.

Keywords:

Alkali Aggregate Reaction (AAR), heavy aggregate, ionizing radiation

Affiliations:
Jóźwiak-Niedźwiedzka D.-IPPT PAN
Brandt A.M.-IPPT PAN
Gibas K.-IPPT PAN
Denis P.-IPPT PAN
6.Kołbuk D., Sajkiewicz P., Denis P., Choińska E., Investigations of polycaprolactone/gelatin blends in terms of their miscibility, BULLETIN OF THE POLISH ACADEMY OF SCIENCES: TECHNICAL SCIENCES, ISSN: 0239-7528, DOI: 10.2478/bpasts-2013-0066, Vol.61, No.3, pp.629-632, 2013
Abstract:

Synthetic and natural polymers blends represent a new brand of materials with application in wound healing, scaffolds or drug delivery systems. Polycaprolactone/gelatin (PCL/Gt) blends were analyzed in terms of their miscibility. The PCL structure was investigated as a function of Gt content. Changes in the PCL spherulitic structure with Gt content were investigated by a polarizing-interference microscope. The analysis of the glass transition temperature (Tg) of both components as a function of PCL/Gt ratio by differential scanning calorimetry indicates that the system of polycaprolactone/gelatin belongs to a type of s.c. compatible system, being intermediate between miscible and immiscible systems. There is possibility of very limited miscibility of both components. Supplementary wide angle X-ray scattering results are presented.

Keywords:

lends, compatibility, miscibility, polycaprolactone, gelatin

Affiliations:
Kołbuk D.-IPPT PAN
Sajkiewicz P.-IPPT PAN
Denis P.-IPPT PAN
Choińska E.-Warsaw University of Technology (PL)

List of chapters in recent monographs
1.
351
Brandt A.M., Jóźwiak-Niedźwiedzka D., Nowowiejski G., Denis P., Dni betonu, Tradycja i Nowoczesność, rozdział: Wyniki badania betonu osłonowego z kruszywem magnetytowym, SPC Stowarzyszenie Producentów Cementu, pp.839-850, 2014

Conference papers
1.Jóźwiak-Niedźwiedzka D., Glinicki M.A., Gibas K., Jaskulski R., Denis P., Garbacik A., Alkali-silica expansion of heavy aggregates used for nuclear shielding concrete, BMC-11, 11th International Symposium on Brittle Matrix Composites, 2015-09-28/09-30, Warsaw (PL), pp.353-360, 2015
Abstract:

In the present study the potential appearance of the alkali-silica reaction (ASR) in heavy aggregates was studied. ASTM C1260 Standard Test Method for Potential Alkali Reactivity of Aggregates (Mortar-Bar Method) was applied. In order to investigate the effect of the content of alkalies in cement on the expansions due to ASR, three levels of total and soluble alkali content of cement were studied. Three portland cements Type I with different alkali content were selected. Two ordinary portland cements, which are commonly available in the market and one special cement were tested. That cement was specially made for the purpose of the nuclear shielding concrete CEM I NA-SR-LH of low-alkali, increased sulphate resistance and low heat of hydration. For the tests according to ASTM C 1260 the high-density aggregates, known as absorbing gamma radiation were selected: barite, magnetite and hematite. The expansion test revealed that hematite was highly reactive, regardless of the type of cement. Already after four days of storage in 1 N NaOH and 80°C the mortar bar expansion exceeded the limit of 0.1%, and after next four days was more than 0.2%., which qualifies it extremely reactive aggregate. Other aggregates after 14 days of testing did not exceed 0.1% elongation limit, but the influence of the type of cement was noticed. There was a noticeable tendency for increasing the total expansion with increasing the alkali content of cement.

Keywords:

Alkali-Silica Reaction (ASR), high density aggregate, cement composition

Affiliations:
Jóźwiak-Niedźwiedzka D.-IPPT PAN
Glinicki M.A.-IPPT PAN
Gibas K.-IPPT PAN
Jaskulski R.-IPPT PAN
Denis P.-IPPT PAN
Garbacik A.-Institute of Ceramics and Building Materials (PL)
2.Jóźwiak-Niedźwiedzka D., Gibas K., Brandt A.M., Glinicki M.A., Dąbrowski M., Denis P., Mineral composition of heavy aggregates for nuclear shielding concrete in relation to alkali-silica reaction, Procedia Engineering, ISSN: 1877-7058, DOI: 10.1016/j.proeng.2015.06.132, Vol.108, pp.162-169, 2015
Abstract:

The results of microscopic analysis on thin sections of aggregates intended for nuclear shielding concrete are presented. The petrographic analysis was performed on different barite, magnetite and hematite aggregates used as the high-density aggregate to absorb gamma radiation. Both optical microscopy and accelerated expansion tests were used to recognise the potential for alkali-reactivity of the aggregates. The mineral composition of the tested aggregates was analysed on thin sections in transmitted cross-polarized light and XRD tests were additionally performed. Expansion tests of mortar bars exposed to 1 N NaOH solution at the temperature of 80°C were also performed following ASTM C1260. The high-density aggregates contained ore deposits - metallic opaque phases providing the required shielding properties. These aggregates also contained various amounts of non-metallic phases, with both innocuous non-ore minerals and deleterious, potentially reactive materials. Barite aggregates contained siderite, fluorite and hawleyite in varying amounts, apart from the barium sulphate being the principal component. Moreover, particularly reactive cristobalite and muscovite were identified in barite aggregates from certain quarries. Tests according to ASTM C1260 performed on mortars showed the expansion above the accepted criterion for non-reactive aggregate and confirmed the threat due to the presence of cristobalite in barite aggregates.

Keywords:

high-density aggregate, heavy concrete, alkali-silica reaction, thin section, XRD

Affiliations:
Jóźwiak-Niedźwiedzka D.-IPPT PAN
Gibas K.-IPPT PAN
Brandt A.M.-IPPT PAN
Glinicki M.A.-IPPT PAN
Dąbrowski M.-IPPT PAN
Denis P.-IPPT PAN
3.Jóźwiak-Niedźwiedzka D., Gibas K., Brandt A.M., Denis P., Influence of barite composition on potential alkali aggregate reaction in radiation-shielding concrete, TINCE 2014, 2nd International Conference on Technological Innovations in Nuclear Civil Engineering, 2014-09-01/09-04, Paryż (FR), pp.1-12, 2014
Abstract:

In the paper the barite aggregate has been analyzed as a potential source of ASR in heavy concrete. Special attention was paid to BaSO4 and minor mineral components in aggregates and their influence on ASR development in tested mortars

Keywords:

heavy aggregate, Alkali Silica Reaction (ASR), ionizing radiation

Affiliations:
Jóźwiak-Niedźwiedzka D.-IPPT PAN
Gibas K.-IPPT PAN
Brandt A.M.-IPPT PAN
Denis P.-IPPT PAN
4.Kołbuk D., Denis P., Choińska E., Sajkiewicz P., Investigations of polycaprolactone/gelatine blends in terms of their miscibility, 3rd Polish Conference on Nano- and Micromechanics, 2012-07-04/07-07, Warszawa (PL), Vol.1, pp.135-137, 2012

Conference abstracts
1.Chrzanowska J., Hoffman J., Mościcki T., Denis P., Szymański Z., Comparison of tungsten boride layers deposite by laser pulse, magnetron sputtering and combined magnetron sputtering-pulsed laser deposition, COLA 2017, International Conference on Laser Ablation, 2017-09-03/09-08, Marseille (FR), pp.202-202, 2017
2.Chrzanowska J., Garbiec D., Kurpaska Ł., Denis P., Hoffman J., Mościcki T., Szymański Z., The effect of substrate temperature on the properties of tungsten boride layers deposited by radio frequency magnetron sputtering and pulsed laser deposition, EYEC, 6th European Young Engineers Conference, 2017-04-24/04-26, Warszawa (PL), pp.240-240, 2017
Keywords:

RF magnetron sputtering, hard materials, PLD, tungsten boride

Affiliations:
Chrzanowska J.-IPPT PAN
Garbiec D.-other affiliation
Kurpaska Ł.-National Centre for Nuclear Research (PL)
Denis P.-IPPT PAN
Hoffman J.-IPPT PAN
Mościcki T.-IPPT PAN
Szymański Z.-IPPT PAN
3.Dulnik J., Kołbuk D., Denis P., Sajkiewicz P., Cellular studies of electrospun PCL/biocomponent nanofibers from alternative and traditional solvents, TERMIS-EU 2017, European Chapter Meeting of the Tissue Engineering and Regenerative Medicine International Society 2017, 2017-06-26/06-30, Davos (CH), pp.P715, 2017
4.Kołbuk D., Denis P., Urbanek O., Tailoring of mechanical properties by molecular orientation in polymeric scaffolds, BioMaH, BIOMATERIALS FOR HEALTHCARE: Biomaterials for Tissue and Genetic Engineering and the Role of Nanotechnology, 2016-10-17/10-20, Rzym (IT), pp.443-446, 2016
Keywords:

molecular orientation, scaffolds, fibres, tissue engineering

Affiliations:
Kołbuk D.-IPPT PAN
Denis P.-IPPT PAN
Urbanek O.-IPPT PAN
5.Dulnik J., Denis P., Sajkiewicz P., Kołbuk D., Bicomponent PCL/Biopolymer nanofibers electrospun from various solvents - cellular and biodegradation studies, ELECTROSPIN 2016, 4th International Conference on Electrospinning, 2016-06-28/07-01, Otranto (IT), pp.1, 2016
Abstract:

Electrospun nonwovens from PCL/gelatin and PCL/collagen structurally mimic native extracellular matrix and provide cells with chemical cues affecting them. Electrospinning of bicomponent nanofibres requires the use of a solvent which dissolves both of the polymers.
We have optimized the process of electrospinning of PCL/gelatin and PCL/collagen nanofibers based on the use of non-toxic, alternative solvents: acetic acid and formic acid (AA/FA) as previously described [1].
Bicomponent PCL/gelatin and PCL/collagen nanofibers were formed by electrospinning using the mixture of acetic acid and formic acid (9:1 w/w ratio), while hexafluoroisopropanol (HFIP) was used as a reference solvent. Nonwoven materials were subjected to cellular in vitro and biodegradation tests and compared.
All in vitro tests were performed using L929 mouse fibroblast cells. Cytotoxicity test was carried out on extracts and showed that all type of materials are not cytotoxic. Materials with 10% biopolymer content as well as made from PCL only underwent experiment in direct contact. Cells were cultured on materials for 3, 5 and 7 days and afterwards taken for SEM as well as fluorescent dying of nuclei and cytoskeleton. Obtained results proved that the addition of Arg-Gly-Asp (RGD) amino acid sequences from biopolymer, in comparison to pure PCL materials, facilitates cell adhesion and spreading on the surface of nonwovens regardless of solvent used in electrospinning.
PCL/gelatin and PCL/collagen nonwovens underwent biodegradation in PBS solution at 37°C. After different times, ranging from 1 to 90 days samples were subjected for comparative analysis via various methods.
Despite the fact that bicomponent nanofibers electrospun from alternative solvents have similar morphology to those electrospun from perfluorinated alcohols, they differ in the internal structure which seriously affects biodegradation process. Biodegradation of investigated materials is manifested mainly by the gelatin leaching, which leads to nanofibers erosion, particularly large for nanofibers spun from AA/FA.

Keywords:

electrospinning, bicomponent nanofibers, biodegradation, cellular studies

Affiliations:
Dulnik J.-IPPT PAN
Denis P.-IPPT PAN
Sajkiewicz P.-IPPT PAN
Kołbuk D.-IPPT PAN
6.Chrzanowska J., Denis P., Mościcki T., Hoffman J., Garbiec D., Frąś L.J. , Szymański Z., Characterization of tungsten boride layers deposited in pulsed laser ablation process, SolMech 2016, 40th Solid Mechanics Conference, 2016-08-29/09-02, Warszawa (PL), No.P257, pp.1-2, 2016
Abstract:

Tungsten boride compounds are very promising new hard and super - hard materials. This kind of materials could be used in production of high-speed tools, durable bearings and nonabrasive surfaces. The hardness of tungsten borides strongly depends on boron content as well as material microstructure. For example hardness of W2B is about 12.4 GPa, WB hardness is 18 – 36 GPa, WB2 hardness is 28.5–39.7 GPa and WB3 hardness is 28.6 – 36.9 GPa. Moreover production of tungsten borides in the form of layers or nano -grained structured increase its hardness even above 40 GPa. Although the properties of tungsten boride structures are known from theoretical calculations, the data concerning structures produced experimentally are scare. Therefore, the properties of tungsten boride layers deposited by pulsed laser deposition (PLD) process are investigated in this paper. Layers were deposited using two types of targets: with boron to tungsten ratio of 2.5:1 and 4.5:1 and with the use of two laser wavelengths: 355 and 1064 nm.

Keywords:

pulsed laser deposition, tungsten borides

Affiliations:
Chrzanowska J.-IPPT PAN
Denis P.-IPPT PAN
Mościcki T.-IPPT PAN
Hoffman J.-IPPT PAN
Garbiec D.-other affiliation
Frąś L.J. -IPPT PAN
Szymański Z.-IPPT PAN
7.Dulnik J., Denis P., Sajkiewicz P., Kołbuk D., Biodegradation of bicomponent PCL/Gelatin nanofibres electrospun from alternative solvent system. Structure and properties analysis, Electrospun Nano- and Microfibres for Biomedical Applications Conference, 2015-08-31/09-03, Eger (HU), pp.1, 2015
Abstract:

Bicomponent polycaprolactone/gelatin nanofibers were formed by electrospinning as previously described [1] using a novel polymer – solvent system with solvents being alternative to the commonly used toxic solvents like fluorinated alcohols. PCL/Gelatin nanofibres were electrospun from the mixture of acetic acid (AA) with formic acid (FA) (90:10) and from hexafluoroisopropanol (HFIP), that was used as reference solvent. PCL/Gelatin nanofibres with polymers w/w ratios 9:1, 8:2 and 7:3, underwent biodegradation in PBS solution at 37°C. After different times, ranging from 1 to 90 days, they were rinsed in demineralized water and dried. Weight loss and FTIR tests were performed to assess the kinetics of gelatin leaching, while SEM imaging and hydrophobicity tests to show its depletion from the surface. DSC measurements were carried out to examine changes in fibres’ internal structure and uniaxial tensile testing to compare their mechanical properties. Morphology of PCL/Gt fibers obtained from AA/FA is similar to that obtained from HFIP. Despite similar morphology, the internal structure of nanofibers formed from alternative solvents is different, reflecting the emulsive nature of PCL/gelati n mixture in AA/FA solvents contrary to clear, transparent solutions in HFIP. This apparent difference affects strongly the kinetics of leaching of gelatin from bicomponent fibres and thus how their mechanical and bioactive properties are changing in time after placing in living organism. There is substantial difference in kinetics of gelatin leaching depending on solvent used. Mass measurements show much faster gelatin degradation in nanofibres electrospun from AA/FA than from HFIP . For instance, for PCL/Gt 7:3 samples, gelatin content loss is 85% for AA/FA and 68% for HFIP after 90 days. Moreover, irrespective of the solvent used, the degradation rate increases with initial content of gelatin and is the highest in the first 24 hours: 27% for AA/FA 9:1 and 67% for 7:3 and 13% and 32% for HFIP respectively. The observed changes can be explained by nonuniform distribution of gelatin within fibres spun form AA/FA due to emulsive character of solution. Comparison of SEM images reveals linear groove-like sites remaining after gelatin leaching on a surface of fibres spun from AA/FA solvent. Contrary to this, fibres spun from HFIP remain smooth which can be attributed to molecular dispersion of both components.

Keywords:

nanofibers, biodegradation, polycaprolactone, gelatin

Affiliations:
Dulnik J.-IPPT PAN
Denis P.-IPPT PAN
Sajkiewicz P.-IPPT PAN
Kołbuk D.-IPPT PAN
8.Kołbuk D., Denis P., Bil M., Sajkiewicz P., Influence of Crystallinity and Selected Mechanical Properties on Cellular Response, ICMAT2015, 8th International Conference on Materials for Advanced Technologies and IUMRS – International Conference in Asia, 2015-06-28/07-03, Suntec (SG), No.M-PO2, 2015
Abstract:

Cells sense subtract stiffness, elasticity and transduce that information into morphological changes and lineage specification. Polymer molecular order and mechanical properties, specially stiffness and elasticity indicate influence on cellular response during in- vitro study [e.g. Bershadskye et al 2013]. The aim of proposed presentation is to evaluate the effect of tailored crystallinity and mechanical properties of one- and bicomponent polymer films in terms of cells morphology and proliferation without changing other parameters. Polycaprolactone (PCL) and Gelatin (Ge) were used. As a solvents: Hexafluoroisopropanole (H), Acetic Acid (AA) were chosen. Two methods of foil preparation were analysed: forming from melt (onecomponent), forming from solution (one- and bicomponent).In both methods, the degree of crystallinity was modified mainly by the different type of PCL molecular weight, solvent type and/or annealing. Films were analysed using polarizinginterference microscopy allowing characterization of spherulities morphology. Degree of crystallinity was analysed by differential scanning calorimetry. Foils topography was analysed by atomic force microscopy, selected mechanical properties and hydrophilicity (contact angle) as the significant from the viewpoint of cellular activity were determined as well. L929 cells adhesion and morphology ware analysed by immunohistochemical staining for actin and nuclei. Cell activity and proliferation were analysed also. It is evident that conditions of PCL films preparation affect the morphology of spherulites. All samples were birefringent, indicating in general crystallinity, being different for particular samples. Maltese cross was observed in few samples. Crystallinity of PCL films determined from DSC measurements was in range 0,45-0,70 depending on solvent and polymer molecular weight used. Young Modulus strongly depends on Mw of PCL and Ge additive. L929 cells interact with subtract; morphology and proliferation degree change with crystallinity and elasticity of one- and bicomponet films.

Keywords:

crystallinity, PCL, mechanical properties, casted films

Affiliations:
Kołbuk D.-IPPT PAN
Denis P.-IPPT PAN
Bil M.-Warsaw University of Technology (PL)
Sajkiewicz P.-IPPT PAN
9.Chrzanowska J., Hoffman J., Denis P., Mościcki T., The effect of substrate heating on the ReB2 layers deposited by PLD method, ITFPC'2015, 7th International Conference on Innovations in Thin Film Processing and Characterization, 2015-11-16/11-20, Nancy (FR), pp.93, 2015
Keywords:

pulsed laser deposition, rhenium borides

Affiliations:
Chrzanowska J.-IPPT PAN
Hoffman J.-IPPT PAN
Denis P.-IPPT PAN
Mościcki T.-IPPT PAN
10.Kołbuk D., Denis P., Dulnik J., Sajkiewicz P., Modifications of polycaprolactone films crystallinity in terms of tissue engineering applications, 20th Swiss Conference on Biomaterials and Regerative Medicine, 2014-05-07/05-08, Basel (CH), Vol.28, No.6, pp.30, 2014
Abstract:

Few research groups have highlighted the unexpected degree of cell proliferation depending on the degree of crystallinity of the substrate. Commonly used methods of forming three-dimensional scaffolds do not take into account crystallinity optimisation.
The aim of proposed presentation is to investigate polycaprolactone (PCL) substrate supermolecular structure effect, mainly crystallinity, on cells spreading, activity and proliferation.

Keywords:

crystalllinity, PCL, solvent, molecular structure, L929

Affiliations:
Kołbuk D.-IPPT PAN
Denis P.-IPPT PAN
Dulnik J.-IPPT PAN
Sajkiewicz P.-IPPT PAN
11.Denis P., Dulnik J., Sajkiewicz P., Electrospinning and structure of bicomponent polycaprolactone/gelatin nanofibers obtained using alternative solvent system, 2nd INTERNATIONAL CONFERENCE ON BIO-BASED POLYMERS AND COMPOSITES, 2014-08-24/08-28, Visegrad (HU), pp.1-2, 2014
Abstract:

In this study bicomponent polycaprolactone/gelatin nanofibers were successfully formed by electrospinning using for the first time a novel polymer – solvent system consisting of acetic acid and formic acid. Such solvent system is alternative to the commonly used toxic solvents like fluorinated alcohols, mainly hexafluoroisopropanol. The effect of electrospinning conditions on morphology and structure of nanofibers were investigated.

Keywords:

nanofibers, electrospinning, polycaprolactone, gelatin, alternative solvents, structure

Affiliations:
Denis P.-IPPT PAN
Dulnik J.-IPPT PAN
Sajkiewicz P.-IPPT PAN
12.Kołbuk D., Denis P., Choińska E., Sajkiewicz P., Tailoring of polycaprolactone crystallinity, E-MRS FALL MEETING 2014, 2014-09-15/09-18, Warszawa (PL), pp.91, 2014
Abstract:

Introduction In the case of semicrystalline polymers, crystallinity is the parameter determining their physical properties. Some research groups indicate influence of crystallinity on cells response during in- vitro study. Commonly used methods of three-dimensional scaffolds formation do not take into account crystallinity optimisation. The aim of proposed presentation is to evaluate the effect of molecular weight and solvent on crystallinity and crystal size in case of polycaprolactone (PCL) films. Methodology Material: PCL with Mn:10, 45 and 80k g/mol (Sigma Aldrich) was used. As a solvents: Hexafluoroisopropanole, HFIP (Iris Biotech GmbH.), Acetic Acid, AA and Dichloromethane, DCM (Avantor and Chempol respectively) were used. Methods: Films were prepared from the PCL with different molecular weight using various solvents differing in evaporation rate. Characterization: Films were analysed using polarizing-interference microscopy (MPI) allowing characterization ofspherulities morphology. Degree of crystallinity was analysed by differential scanning calorimetry (DSC) and comparatively bywide angle X-ray scattering (WAXS). Results and Discussion It is evident from MPI observations that conditions of PCL films preparation affect the morphology of spherulites. All samples were birefringent, indicating in general crystallinity, being different for particular samples. Sphorulities size depends on Mw and solvent type; sharp Maltese cross was observed on few samples. Crystallinity of PCL films determined from DSC measurements was in the range 0,45-0,68 depending on solvent and polymer Mn used. Generally crystallinity of films formed from DCM is lower than from AA as a result of lower boiling point of DCM. Additional annealing enables increase in crystallinity to 0,8. WAXS crystallinity correlates with values determined by DSC. Changes of full width of half maximum(FWHM) of crystal peaks indicate variations of crystal size and/or defects depending on molecular weight and solvent what correlates with MPI observations also. Conclusions spherulites shape and crystallinity are strongly dependent on Mn and solvent type. Structural parameters of films decide on Young modulus and elasticity in terms of applications

Keywords:

crystallinity, PCL, solvents WAXS, molecular structure

Affiliations:
Kołbuk D.-IPPT PAN
Denis P.-IPPT PAN
Choińska E.-Warsaw University of Technology (PL)
Sajkiewicz P.-IPPT PAN
13.Kołbuk D., Denis P., Urbanek O., L929 response on polycaprolactone films with tailored crystallinity, Szkoła Zimowa, 2014-12-15/12-16, Warszawa (PL), pp.29, 2014
Abstract:

Influence of the crystallinity of the substrate on cell proliferation during in-vitro study was highlighted in few articles. Methods of forming 3-D scaffolds usually do not take into account crystallinity optimisation. The aim of proposed presentation is to investigate an influence of polycaprolactone (PCL) crystallinity on cells spreading, their activity and proliferation. PCL Mn 45k and Mn 80k g/mol were used. As a solvents: HFIP (H) and Acetic Acid (AA) were used. Two methods of foil preparation were analysed: -forming from melt (PCL45, PCL80) -forming from 10%wt solution (e.g. PH45, PAA45) Samples were analyzed using interfered-polarization microscopy (MIP) which allows to describe the morphology of spherolites (crystalline and amorphous phase). Degree of crystallinity was analysed by differential scanning calorimetry (DSC) and wide angle X-ray scattering (WAXS). Selected samples surfaces were O2 plasma treated to decrease hydrophobic properties of PCL. L929 cells adhesion and morphology were analyzed by immunohistochemical staining for actin and nuc lei. Cell activity and proliferation were analyzed. Morphology of spherolites was analyses using interfered-polarization microscopy (MIP). Analyses indicate changes in spherolites shape, size and also crystalline/ amorphous phase amount. Differences of crystallinity for PCL using different molecular weight were analysed by DSC and WAXS measurements. Decrease of contact angle was observed for O2 plasma treated samples. All PCL films were found as nontoxic for L929 cells. Differences in cells spreading, activity and proliferation degree were found. Modification of Mn, solvent and concentration of PCL enable film formation in wide range of crystallinity. L929 during in-vitro study interact with the PCL film. Crystallinity as part of the supermolecular structure influence on cells morphology.

Keywords:

crystallinity, PCL, cellular responce, structure

Affiliations:
Kołbuk D.-IPPT PAN
Denis P.-IPPT PAN
Urbanek O.-IPPT PAN
14.Alhazov D., Gradys A., Denis P., Sajkiewicz P., Arinstein A., Zussman E., Thermo-mechanical behavior of electrospun thermoplastic polyurethane nanofibers, EPF2013, European Polymer Congress, 2013-06-16/06-21, Pisa (IT), pp.O2-23, 2013

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414353
2015-10-13
BUP 9/2017
2017-04-24
Chwojnowski A., Łukowska E., Wojciechowski C., Gadomska-Gajadhur A., Kruk A., Ruśkowski P., Synoradzki L., Denis P., Dulnik J., Sajkiewicz P.
Sposób otrzymywania szerokoporowatego, poliestrowego rusztowania komórkowego
PL, Instytut Biocybernetyki i Inżynierii Biomedycznej im. Macieja Nałęcza PAN, Politechnika Warszawska, Instytut Podstawowych Problemów Techniki PAN
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