Instytut Podstawowych Problemów Techniki
Polskiej Akademii Nauk

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M. Bryliński


Ostatnie publikacje
1.  Bryliński M., Prymula K., Jurkowski W., Kochańczyk M., Stawowczyk E., Konieczny L., Roterman I., Prediction of Functional Sites Based on the Fuzzy Oil Drop Model, PLOS COMPUTATIONAL BIOLOGY, ISSN: 1553-7358, DOI: 10.1371/journal.pcbi.0030094, Vol.3, No.5, pp.e94-0909-0923, 2007

Streszczenie:
A description of many biological processes requires knowledge of the 3-D structure of proteins and, in particular, the defined active site responsible for biological function. Many proteins, the genes of which have been identified as the result of human genome sequencing, and which were synthesized experimentally, await identification of their biological activity. Currently used methods do not always yield satisfactory results, and new algorithms need to be developed to recognize the localization of active sites in proteins. This paper describes a computational model that can be used to identify potential areas that are able to interact with other molecules (ligands, substrates, inhibitors, etc.). The model for active site recognition is based on the analysis of hydrophobicity distribution in protein molecules. It is shown, based on the analyses of proteins with known biological activity and of proteins of unknown function, that the region of significantly irregular hydrophobicity distribution in proteins appears to be function related.

Słowa kluczowe:
Protein structure, Amino acid analysis, Isomerases, Protein structure prediction, Genomic databases, Structural genomics, Protein structure comparison, Oils

Afiliacje autorów:
Bryliński M. - inna afiliacja
Prymula K. - inna afiliacja
Jurkowski W. - inna afiliacja
Kochańczyk M. - IPPT PAN
Stawowczyk E. - inna afiliacja
Konieczny L. - inna afiliacja
Roterman I. - Jagiellonian University (PL)
2.  Bryliński M., Kochańczyk M., Broniatowska E., Roterman I., Localization of ligand binding site in proteins identified in silico, Journal of Molecular Modeling, ISSN: 1610-2940, DOI: 10.1007/s00894-007-0191-x, Vol.13, No.6, pp.665-675, 2007

Streszczenie:
Knowledge-based models for protein folding assume that the early-stage structural form of a polypeptide is determined by the backbone conformation, followed by hydrophobic collapse. Side chain–side chain interactions, mostly of hydrophobic character, lead to the formation of the hydrophobic core, which seems to stabilize the structure of the protein in its natural environment. The fuzzy-oil-drop model is employed to represent the idealized hydrophobicity distribution in the protein molecule. Comparing it with the one empirically observed in the protein molecule reveals that they are not in agreement. It is shown in this study that the irregularity of hydrophobic distributions is aim-oriented. The character and strength of these irregularities in the organization of the hydrophobic core point to the specificity of a particular protein’s structure/function. When the location of these irregularities is determined versus the idealized fuzzy-oil-drop, function-related areas in the protein molecule can be identified. The presented model can also be used to identify ways in which protein–protein complexes can possibly be created. Active sites can be predicted for any protein structure according to the presented model with the free prediction server at http://www.bioinformatics.cm-uj.krakow.pl/activesite. The implication based on the model presented in this work suggests the necessity of active presence of ligand during the protein folding process simulation.

Słowa kluczowe:
Hydrophobic collapse, Protein folding, Active site, Ligand binding

Afiliacje autorów:
Bryliński M. - inna afiliacja
Kochańczyk M. - IPPT PAN
Broniatowska E. - inna afiliacja
Roterman I. - Jagiellonian University (PL)
3.  Bryliński M., Kochańczyk M., Konieczny L., Roterman I., Sequence-structure-function relation characterized in silico, In Silico Biology, ISSN: 1386-6338, Vol.6, No.6, pp.589-600, 2006

Słowa kluczowe:
Hydrophobicity, biological function, active site, proteins of unknown biological function

Afiliacje autorów:
Bryliński M. - inna afiliacja
Kochańczyk M. - IPPT PAN
Konieczny L. - inna afiliacja
Roterman I. - Jagiellonian University (PL)

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