Instytut Podstawowych Problemów Techniki
Polskiej Akademii Nauk

Partnerzy

Paweł Ruśkowski

Warsaw University of Technology (PL)


Ostatnie publikacje
1.  Budnicka M., Szymaniak M., Kołbuk D., Ruśkowski P., Gadomska-Gajadhur A., Biomineralization of poly-l-lactide spongy bone scaffolds obtained by freeze-extraction method, Journal of Biomedical Materials Research Part B: Applied Biomaterials, ISSN: 1552-4973, DOI: 10.1002/jbm.b.34441, Vol.108, No.3, pp.868-879, 2020

Streszczenie:
Implants in the form of polymer scaffolds are commonly used to regenerate bone tissue after traumas or tooth extractions. However, few implant formation methods enable building polymer scaffolds allowing to reconstruct larger bone losses without immune response. Spacious, porous poly-l-lactide implants with considerable volume were obtained using the phase inversion method with the freeze-extraction variant. The calcium phosphate (CaP) coating was deposited on implant surfaces with the biomimetic method to improve the implant's osteoconductivity. The substitues morphology was characterized-porosity, size and shape of pores; mechanical properties, mass absorbability of implants before and after mineralization. The characteristics were provided with scanning electron microscopy (SEM), static compression test and hydrostatic weighing, respectively. The presence of CaPs in the entire volume of the implant was confirmed with SEM and infrared spectroscopy with Fourier transform (FTIR). The biocompatibility of scaffolds was confirmed with in vitro quantitative test and microscopic observations. The obtained results show that the implants can be used in tissue engineering as a vehicle of platelet-rich plasma to regenerate critical spongy bone losses.

Słowa kluczowe:
bone graft, calcium phosphate(s), cell culture

Afiliacje autorów:
Budnicka M. - inna afiliacja
Szymaniak M. - inna afiliacja
Kołbuk D. - IPPT PAN
Ruśkowski P. - Warsaw University of Technology (PL)
Gadomska-Gajadhur A. - Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)
140p.
2.  Budnicka M., Kołbuk D., Ruśkowski P., Gadomska‐Gajadhur A., Poly‐L ‐lactide scaffolds with super pores obtained by freeze‐extraction method, Journal of Biomedical Materials Research Part B: Applied Biomaterials, ISSN: 1552-4973, DOI: 10.1002/jbm.b.34642, Vol.108, No.8, pp.3162-3173, 2020

Streszczenie:
A nonplanar polylactide scaffold to be used in tissue engineering was obtained by freeze‐extraction method. Properties of the scaffold were modified by adding Eudragit® E100. The impact of the modification on morphology, porosity and pore size, mass absorbability, mechanical properties was determined. Scanning electron microscopy (SEM), hydrostatic weighing test, static compression test was used to this end. The chemical composition of the scaffold was defined based on infrared spectroscopy (FTIR) and energy‐dispersive X‐ray spectroscopy (EDX). Biocompatibility was confirmed by quantitative tests and microscopic observation. The obtained results show that the obtained scaffolds may be applied as a carrier of hydrophilic cellular growth factors for more efficient tissue regeneration.

Słowa kluczowe:
cellular studies, Eudragit® E100, freeze-extraction, poly-L-lactide

Afiliacje autorów:
Budnicka M. - inna afiliacja
Kołbuk D. - IPPT PAN
Ruśkowski P. - Warsaw University of Technology (PL)
Gadomska‐Gajadhur A. - Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)
140p.
3.  Gadomska‐Gajadhur A., Kruk A., Ruśkowski P., Sajkiewicz P., Dulnik J., Chwojnowski A., Original method of imprinting pores in scaffolds for tissue engineering, Polymers for Advanced Technologies, ISSN: 1042-7147, DOI: 10.1002/pat.5091, pp.1-13, 2020

Streszczenie:
Results of the preparation of biodegradable porous scaffolds using an original modification of a wet phase inversion method were presented. Influence of gelatin non‐woven as a non‐classic pore precursor and polyvinylpyrrolidone, Pluronic as classic pore precursors on the structure of obtained scaffolds was analyzed. It was shown that the addition of gelatin non‐wovens enables the preparation of scaffolds, which allow for the growth of cells (size, distribution, and shape of pores). Mechanical properties of the obtained cell scaffolds were determined. The influence of pore precursors on mass absorption of scaffolds against isopropanol and plasma was investigated. Interaction of scaffolds with a T‐lymphocyte line (Jurkat) and with fibroblasts (L929) was investigated. Obtained scaffolds are not cytotoxic and can be used as implants, for example, the regeneration of cartilage tissue.

Słowa kluczowe:
cell cultures, cytotoxic, fibroblasts, imprinted scaffolds

Afiliacje autorów:
Gadomska‐Gajadhur A. - inna afiliacja
Kruk A. - Warsaw University of Technology (PL)
Ruśkowski P. - Warsaw University of Technology (PL)
Sajkiewicz P. - IPPT PAN
Dulnik J. - IPPT PAN
Chwojnowski A. - Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)
70p.
4.  Kruk A., Gadomska-Gajadhur A., Dulnik J., Ruśkowski P., The influence of the molecular weight of polymer on the morphology, functional properties and L929 fibroblasts growth on polylactide membranes for tissue engineering, International Journal of Polymeric Materials and Polymeric Biomaterials, ISSN: 0091-4037, DOI: 10.1080/00914037.2020.1798440, pp.1-13, 2020

Streszczenie:
The main goal of tissue engineering (TE) is supporting the regeneration of damaged tissues that are difficult to regenerate. The experimental results of the preparation of semi-permeable membranes for cell cultures are presented. The effect of the PLA molecular weight and addition of pore precursors on the morphology of the membranes was studied. The pore precursor of choice was polyvinylpyrrolidone (PVP). It was found that semi-permeable membranes for application in tissue engineering can be prepared with polylactides of molecular weight more significant than 37,000 g/mol. Moreover, it was observed that the growth of the molecular weight of the polymer, the porosity, the size of the pores, the Young modulus and maximum tensile increased. Additionally, to obtain a better morphology of the membranes, PVP should be added to the polymeric solution. Positive growth of L929 fibroblast cells on the obtained scaffolds was shown.

Słowa kluczowe:
biodegradable polymers, cell cultures, L929 fibroblasts, polylactide, scaffolds, tissue engineering

Afiliacje autorów:
Kruk A. - Warsaw University of Technology (PL)
Gadomska-Gajadhur A. - Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)
Dulnik J. - IPPT PAN
Ruśkowski P. - Warsaw University of Technology (PL)
70p.
5.  Kruk A., Gadomska-Gajadhur A., Rykaczewska I., Dulnik J., Ruśkowski P., Synoradzki L., Influence of liquid pore precursors on morphology and mechanical properties of 3D scaffolds obtained by dry inversion phase method, Journal of Biomedical Materials Research Part B: Applied Biomaterials, ISSN: 1552-4973, DOI: 10.1002/jbm.b.34200, Vol.107, No.4, pp.1079-1087, 2019

Streszczenie:
Polyester 3D scaffolds were obtained by dry inversion phase method. The influence of a polymer and liquid pore precursor type on the 3D scaffolds morphology, porosity and mechanical properties was tested. Polymers and precursors forming a porous structure were identified. It was found that 3D scaffolds having the most preferable structure for cell cultures were obtained from polylactide with the addition of n‐butanol as the liquid pore precursor.

Słowa kluczowe:
liquid pore precursors, mechanical properties, dry inversion phase method, 3D scaffolds

Afiliacje autorów:
Kruk A. - Warsaw University of Technology (PL)
Gadomska-Gajadhur A. - Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)
Rykaczewska I. - Warsaw University of Technology (PL)
Dulnik J. - IPPT PAN
Ruśkowski P. - Warsaw University of Technology (PL)
Synoradzki L. - Warsaw University of Technology (PL)
140p.
6.  Kruk A., Gadomska-Gajadhur A., Dulnik J., Rykaczewska I., Ruśkowski P., Sebai A., Synoradzki L., Ocena właściwości użytkowych rusztowań komórkowych o strukturze gąbczastej oraz wzrostu na nich fibroblastów, POLIMERY, ISSN: 0032-2725, DOI: 10.14314/polimery.2018.4.3, Vol.63, No.4, pp.18-22, 2018

Streszczenie:
Zbadano wpływ dodatku ciekłych prekursorów porów na morfologię, porowatość i właściwości mechaniczne polilaktydowych rusztowań komórkowych. Rusztowania otrzymano metodą mokrej inwersji faz w wariancie freeze extraction. Oceniono cytotoksyczność wybranych rusztowań w stosunku do fibroblastów mysich oraz ich przydatność do hodowli komórkowych. Wykazano, że dodatek prekursora porów dopolilaktydu korzystnie zmienia morfologię wytworzonych rusztowań, jednocześnie pogarszając ich wytrzymałość mechaniczną. Stwierdzono, że polilaktydowe rusztowania komórkowe z powodzeniem mogą być wykorzystywane do hodowli komórkowych.

Słowa kluczowe:
usztowania komórkowe, polilaktyd, hodowle komórkowe, fibroblasty

Afiliacje autorów:
Kruk A. - Warsaw University of Technology (PL)
Gadomska-Gajadhur A. - Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)
Dulnik J. - IPPT PAN
Rykaczewska I. - Warsaw University of Technology (PL)
Ruśkowski P. - Warsaw University of Technology (PL)
Sebai A. - Wroclaw University of Science and Technology (PL)
Synoradzki L. - Warsaw University of Technology (PL)
15p.

Prace konferencyjne
1.  Kruk A., Gadomska-Gajadhur A., Ruśkowski P., Chwojnowski A., Dulnik J., Synoradzki L., Preparation of biodegradable semi-permeable membranes as 3D scaffolds for cell cultures, Desalination and Water Treatment, ISSN: 1944-3994, DOI: 10.5004/dwt.2017.11415, Vol.64, pp.317-323, 2017

Streszczenie:
Results of the preparation of semi-permeable membranes made of biodegradable polymers membranes were presented. Among known polyesters, polylactide was selected for research. The membranes were obtained using wet phase inversion method. The influence of polyvinylpyrrolidone and polymeric nano-non-wovens as pores precursors on the structure of obtained membranes was analysed. It was shown, that utilisation of polymeric nano-non-wovens enabled preparation of semi-permeable membranes, which could be used as wide-pore 3D-type cellular scaffolds.

Słowa kluczowe:
Biodegradable polymers membranes, Biodegradable polyesters, Porous three-dimensional scaffolds, Inversion phase method

Afiliacje autorów:
Kruk A. - Warsaw University of Technology (PL)
Gadomska-Gajadhur A. - Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)
Ruśkowski P. - Warsaw University of Technology (PL)
Chwojnowski A. - Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)
Dulnik J. - IPPT PAN
Synoradzki L. - Warsaw University of Technology (PL)
20p.

Abstrakty konferencyjne
1.  Kruk A., Gadomska-Gajadhur A., Ruśkowski P., Chwojnowski A., Dulnik J., Synoradzki L., Preparation of biodegradable semi-permeable membranes as 3D scaffolds for cell cultures, MEMPEP 2016, XI-th Conference on Membranes and Membrane Processes in Environmental Protection, 2016-06-15/06-18, Zakopane (PL), pp.24, 2016

Patenty
Numer/data zgłoszenia patentowego
Ogłoszenie o zgłoszeniu patentowym
Twórcy
Tytuł
Kraj i Nazwa uprawnionego z patentu
Numer patentu
Ogłoszenie o udzieleniu patentu
pdf
430802
2019-08-02
BUP 03/2021
2021-02-08
Gadomska-Gajadhur A., Budnicka M., Ruśkowski P., Kołbuk-Konieczny D.
Sposób otrzymywania polilaktydowego substytutu kości gąbczastej
PL, Politechnika Warszawska
-
-
-
426830
2018-08-28
BUP 06/2020
2020-03-09
Gadomska-Gajadhur A., Ruśkowski P., Synoradzki L., Wrzecionek M., Kołbuk-Konieczny D., Jeznach O., Budnicka M., Szymaniak M.
Sposób otrzymywania poli(bursztynianiu glicerolu)
PL, Politechnika Warszawska
-
-
-
414353
2015-10-13
BUP 09/2017
2017-04-24
Chwojnowski A., Łukowska E., Wojciechowski C., Gadomska-Gajadhur A., Kruk A., Ruśkowski P., Synoradzki L., Denis P., Dulnik J., Sajkiewicz P.
Sposób otrzymywania szerokoporowatego, poliestrowego rusztowania komórkowego
PL, Instytut Biocybernetyki i Inżynierii Biomedycznej im. Macieja Nałęcza PAN, Politechnika Warszawska, Instytut Podstawowych Problemów Techniki PAN
228884
WUP 05/2018
2018-05-30



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