Instytut Podstawowych Problemów Techniki
Polskiej Akademii Nauk


M. Pyrski

Ostatnie publikacje
1.  Rybka J.D., Mieloch A.A., Plis A., Pyrski M., Pniewski T., Giersig M., Assembly and characterization of HBc derived virus-like particles with magnetic core, Nanomaterials, ISSN: 2079-4991, DOI: 10.3390/nano9020155, Vol.9, No.2, pp.155-1-11, 2019

Core-virus like particles (VLPs) assembly is a kinetically complex cascade of interactions between viral proteins, nanoparticle's surface and an ionic environment. Despite many in silico simulations regarding this process, there is still a lack of experimental data. The main goal of this study was to investigate the capsid protein of hepatitis B virus (HBc) assembly into virus-like particles with superparamagnetic iron oxide nanoparticles (SPIONs) as a magnetic core in relation to their characteristics. The native form of HBc was obtained via agroinfection of Nicotiana benthamiana with pEAQ-HBc plasmid. SPIONs of diameter of 15 nm were synthesized and functionalized with two ligands, providing variety in ζ-potential and hydrodynamic diameter. The antigenic potential of the assembled core-VLPs was assessed with enzyme-linked immunosorbent assay (ELISA). Morphology of SPIONs and core-VLPs was evaluated via transmission electron microscopy (TEM). The most successful core-VLPs assembly was obtained for SPIONs functionalized with dihexadecyl phosphate (DHP) at SPIONs/HBc ratio of 0.2/0.05 mg/mL. ELISA results indicate significant decrease of antigenicity concomitant with core-VLPs assembly. In summary, this study provides an experimental assessment of the crucial parameters guiding SPION-HBc VLPs assembly and evaluates the antigenicity of the obtained structures.

Słowa kluczowe:
virus-like particles, VLPs, hepatitis B virus capsid protein, HBc, viral self-assembly, magnetic core, HBcAg

Afiliacje autorów:
Rybka J.D. - inna afiliacja
Mieloch A.A. - inna afiliacja
Plis A. - inna afiliacja
Pyrski M. - inna afiliacja
Pniewski T. - inna afiliacja
Giersig M. - inna afiliacja

Kategoria A Plus


logo ippt            ul. Pawińskiego 5B, 02-106 Warszawa
  +48 22 826 12 81 (centrala)
  +48 22 826 98 15

Znajdź nas

© Instytut Podstawowych Problemów Techniki Polskiej Akademii Nauk 2024