Instytut Podstawowych Problemów Techniki
Polskiej Akademii Nauk

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Michał Nowacki


Ostatnie publikacje
1.  Jundziłł A., Pokrywczyńska M., Adamowicz J., Kowalczyk T., Nowacki M., Bodnar M., Marszałek A., Frontczak-Baniewicz M.M., Mikułowski G., Kloskowski T., Gatherwright J., Drewa T., Vascularization Potential of Electrospun Poly(L-Lactide-co-Caprolactone) Scaffold: The Impact for Tissue Engineering, Medical Science Monitor, ISSN: 1643-3750, DOI: 10.12659/MSM.899659, Vol.23, pp.1540-1551, 2017

Streszczenie:
BACKGROUND: Electrospun nanofibers have widespread putative applications in the field of regenerative medicine and tissue engineering. When compared to naturally occurring collagen matrices, electrospun nanofiber scaffolds have two distinct advantages: they do not induce a foreign body reaction and they are not at risk for biological contamination. However, the exact substrate, structure, and production methods have yet to be defined. MATERIAL AND METHODS: In the current study, tubular-shaped poly(L-lactide-co-caprolactone) (PLCL) constructs produced using electrospinning technology were evaluated for their potential application in the field of tissue regeneration in two separate anatomic locations: the skin and the abdomen. The constructs were designed to have an internal diameter of 3 mm and thickness of 200 μm. Using a rodent model, 20 PLCL tubular constructs were surgically implanted in the abdominal cavity and subcutaneously. The constructs were then evaluated histologically using electron microscopy at 6 weeks post-implantation. RESULTS: Histological evaluation and analysis using scanning electron microscopy showed that pure scaffolds by themselves were able to induce angiogenesis after implantation in the rat model. Vascularization was observed in both tested groups; however, better results were obtained after intraperitoneal implantation. Formation of more and larger vessels that migrated inside the scaffold was observed after implantation into the peritoneum. In this group no evidence of inflammation and better integration of scaffold with host tissue were noticed. Subcutaneous implantation resulted in more fibrotic reaction, and differences in cell morphology were also observed between the two tested groups. CONCLUSIONS: This study provides a standardized evaluation of a PLCL conduit structure in two different anatomic locations, demonstrating the excellent ability of the structure to achieve vascularization. Functional, histological, and mechanical data clearly indicate prospective clinical utilization of PLCL in critical size defect regeneration.

Słowa kluczowe:
Polymers, Regenerative medicine, Tissue Engineering, Tissue Scaffolds, Urinary Diversion

Afiliacje autorów:
Jundziłł A. - inna afiliacja
Pokrywczyńska M. - inna afiliacja
Adamowicz J. - Nicolaus Copernicus University (PL)
Kowalczyk T. - IPPT PAN
Nowacki M. - inna afiliacja
Bodnar M. - Nicolaus Copernicus University (PL)
Marszałek A. - Nicolaus Copernicus University (PL)
Frontczak-Baniewicz M.M. - Mossakowski Medical Research Centre, Polish Academy of Sciences (PL)
Mikułowski G. - IPPT PAN
Kloskowski T. - inna afiliacja
Gatherwright J. - University Hospitals – Case Medical Center (US)
Drewa T. - Nicolaus Copernicus University (PL)
20p.
2.  Kloskowski T., Jundziłł A., Kowalczyk T., Nowacki M., Bodnar M., Marszałek A., Pokrywczyńska M., Frontczak-Baniewicz M.M., Kowalewski T.A., Chłosta P., Drewa T., Ureter Regeneration–The Proper Scaffold Has to Be Defined, PLOS ONE, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0106023, Vol.9, No.8, pp.106023-1-13, 2014

Streszczenie:
The aim of this study was to compare two different acellular scaffolds: natural and synthetic, for urinary conduit construction and ureter segment reconstruction. Acellular aortic arch (AAM) and poly(L-lactide-co-caprolactone) (PLCL) were used in 24 rats for ureter reconstruction in both tested groups. Follow-up period was 4 weeks. Intravenous pyelography, histological and immunohistochemical analysis were performed. All animals survived surgical procedures. Patent uretero-conduit junction was observed only in one case using PLCL. In case of ureter segment reconstruction ureters were patent in one case using AAM and in four cases using PLCL scaffolds. Regeneration of urothelium layer and focal regeneration of smooth muscle layer was observed on both tested scaffolds. Obtained results indicates that synthetic acellular PLCL scaffolds showed better properties for ureter reconstruction than naturally derived acellular aortic arch.

Słowa kluczowe:
Ureter, Muscle regeneration, Kidneys, Collagens, Urine, Surgical and invasive medical procedures, Smooth muscles, Inflammation

Afiliacje autorów:
Kloskowski T. - inna afiliacja
Jundziłł A. - inna afiliacja
Kowalczyk T. - IPPT PAN
Nowacki M. - inna afiliacja
Bodnar M. - Nicolaus Copernicus University (PL)
Marszałek A. - Nicolaus Copernicus University (PL)
Pokrywczyńska M. - inna afiliacja
Frontczak-Baniewicz M.M. - Mossakowski Medical Research Centre, Polish Academy of Sciences (PL)
Kowalewski T.A. - IPPT PAN
Chłosta P. - Jagiellonian University (PL)
Drewa T. - Nicolaus Copernicus University (PL)
40p.
3.  Pokrywczyńska M., Jundziłł A., Adamowicz J., Kowalczyk T., Warda K., Rasmus M., Buchholz Ł., Krzyżanowska S., Nakielski P., Chmielewski T., Bodnar M., Marszałek A., Dębski R., Frontczak-Baniewicz M.M., Mikułowski G., Nowacki M., Kowalewski T.A., Drewa T., Is the Poly (L- Lactide- Co– Caprolactone) Nanofibrous Membrane Suitable for Urinary Bladder Regeneration?, PLOS ONE, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0105295, Vol.9, No.8, pp.105295-1-12, 2014

Streszczenie:
The purpose of this study was to compare: a new five-layered poly (L–lactide–co–caprolactone) (PLC) membrane and small intestinal submucosa (SIS) as a control in rat urinary bladder wall regeneration. The five-layered poly (L–lactide–co–caprolactone) membrane was prepared by an electrospinning process. Adipose tissue was harvested from five 8-week old male Wistar rats. Adipose derived stem cells (ADSCs) were seeded in a density of 3×106 cells/cm2 onto PLC membrane and SIS scaffolds, and cultured for 5-7 days in the stem cell culture medium. Twenty male Wistar rats were randomly divided into five equal groups. Augmentation cystoplasty was performed in a previously created dome defect. Groups: (I) PLC+ 3×106ADSCs; (II) SIS+ 3×106ADSCs; (III) PLC; (IV) SIS; (V) control. Cystography was performed after three months. The reconstructed urinary bladders were evaluated in H&E and Masson's trichrome staining. Regeneration of all components of the normal urinary bladder wall was observed in bladders augmented with cell-seeded SIS matrices. The urinary bladders augmented with SIS matrices without cells showed fibrosis and graft contraction. Bladder augmentation with the PLC membrane led to numerous undesirable events including: bladder wall perforation, fistula or diverticula formation, and incorporation of the reconstructed wall into the bladder lumen. The new five-layered poly (L–lactide–co–caprolactone) membrane possesses poorer potential for regenerating the urinary bladder wall compared with SIS scaffold.

Słowa kluczowe:
urinary bladder regeneration, electrospinning

Afiliacje autorów:
Pokrywczyńska M. - inna afiliacja
Jundziłł A. - inna afiliacja
Adamowicz J. - Nicolaus Copernicus University (PL)
Kowalczyk T. - IPPT PAN
Warda K. - inna afiliacja
Rasmus M. - Nicolaus Copernicus University (PL)
Buchholz Ł. - Nicolaus Copernicus University (PL)
Krzyżanowska S. - inna afiliacja
Nakielski P. - IPPT PAN
Chmielewski T. - IPPT PAN
Bodnar M. - Nicolaus Copernicus University (PL)
Marszałek A. - Nicolaus Copernicus University (PL)
Dębski R. - Nicolaus Copernicus University (PL)
Frontczak-Baniewicz M.M. - Mossakowski Medical Research Centre, Polish Academy of Sciences (PL)
Mikułowski G. - IPPT PAN
Nowacki M. - inna afiliacja
Kowalewski T.A. - IPPT PAN
Drewa T. - Nicolaus Copernicus University (PL)
40p.
4.  Churski K., Nowacki M., Korczyk P.M., Garstecki P., Simple modular systems for generation of droplets on demand, LAB ON A CHIP, ISSN: 1473-0197, DOI: 10.1039/c3lc50340b, Vol.13, pp.3689-3697, 2013

Streszczenie:
This report provides practical guidelines for the use of inexpensive electromagnetic valves characterized by large dead volumes (tens to hundreds of μL) for the generation of small (nL) droplets on demand in microfluidic chips. We analyze the role of the ratio of resistances and of the elastic capacitance of the fluidic connectors between the reservoir of the liquid, the valve and the microfluidic chip in the reliable and precise formation of micro droplets on demand. We also demonstrate and examine the use of conventional electromagnetic squeeze valves in the generation of small droplets on demand with a similar set of design rules.

Słowa kluczowe:
microfluidics, droplets

Afiliacje autorów:
Churski K. - Institute of Physical Chemistry, Polish Academy of Sciences (PL)
Nowacki M. - inna afiliacja
Korczyk P.M. - IPPT PAN
Garstecki P. - Institute of Physical Chemistry, Polish Academy of Sciences (PL)
40p.
5.  Kloskowski T., Kowalczyk T., Nowacki M., Drewa T., Tissue engineering and ureter regeneration: Is it possible?, INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS, ISSN: 0391-3988, DOI: 10.5301/ijao.5000130, Vol.36, No.6, pp.392-405, 2013

Streszczenie:
Large ureter damages are difficult to reconstruct. Current techniques are complicated, difficult to perform, and often associated with failures. The ureter has never been regenerated thus far. Therefore the use of tissue engineering techniques for ureter reconstruction and regeneration seems to be a promising way to resolve these problems. For proper ureter regeneration the following problems must be considered: the physiological aspects of the tissue, the type and shape of the scaffold, the type of cells, and the specific environment (urine). 
This review presents tissue engineering achievements in the field of ureter regeneration focusing on the scaffold, the cells, and ureter healing.

Afiliacje autorów:
Kloskowski T. - inna afiliacja
Kowalczyk T. - IPPT PAN
Nowacki M. - inna afiliacja
Drewa T. - Nicolaus Copernicus University (PL)
20p.
6.  Nowacki M., Jundziłł A., Bieniek M., Kowalczyk T., Kloskowski T., Drewa T., Nowoczesne biomateriały jako opatrunki hemostatyczne w chirurgii oszczędzającej miąższ nerki-model zwierzęcy. Doniesienie wstępne., POLIMERY W MEDYCYNIE, ISSN: 0370-0747, Vol.42, No.1, pp.35-43, 20125p.

Abstrakty konferencyjne
1.  Kowalczyk T., Niemczyk B., Kloskowski T., Jundziłł A., Adamowicz J., Nowacki M., Pokrywczyńska M., Noszczyk B., Drewa T., Investigation of the in vivo behavior of membranes made of electrospun micro and nanofibers implanted on an animal model, CNM 2019, 6th CONFERENCE ON NANO- AND MICROMECHANICS, 2019-07-03/07-05, Rzeszów (PL), pp.137-139, 2019

Słowa kluczowe:
electrospinning, microfibers, nanofibers, in vivo, animal model

Afiliacje autorów:
Kowalczyk T. - IPPT PAN
Niemczyk B. - IPPT PAN
Kloskowski T. - inna afiliacja
Jundziłł A. - inna afiliacja
Adamowicz J. - Nicolaus Copernicus University (PL)
Nowacki M. - inna afiliacja
Pokrywczyńska M. - inna afiliacja
Noszczyk B. - Medical University of Warsaw (PL)
Drewa T. - Nicolaus Copernicus University (PL)

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