Instytut Podstawowych Problemów Techniki
Polskiej Akademii Nauk

Pracownicy

dr hab. inż. Tomasz Kowalczyk

Samodzielna Pracownia Polimerów i Biomateriałów (SPPiB)
stanowisko: adiunkt
telefon: (+48) 22 826 12 81 wewn.: 423
pokój: 328
e-mail:
ORCID: 0000-0003-2381-4122
strona www: http://pmbm.ippt.pan.pl/web/Tomasz_Kowalczyk

Doktorat
2002-07-02 Badania nad syntezą i polimeryzacją alifatycznych węglanów cyklicznych  (PW)
promotor -- Gabriel Andrzej Rokicki, PW
 
Habilitacja
2020-03-03 Badania nad otrzymywaniem mikro- i nanowłókien metodą elektroprzędzenia oraz ich biologicznymi i medycznymi zastosowaniami 
Promotor prac doktorskich
1.  2015-02-03
pomocniczy
Nakielski Paweł  
(IBIB PAN)
Systemy uwalniania leków oparte na nanowłóknach 

Ostatnie publikacje
1.  Kowalczyk T., Functional micro- and nanofibers obtained by nonwoven post-modification, Polymers, ISSN: 2073-4360, DOI: 10.3390/polym12051087, Vol.12, No.5, pp.1087-1-20, 2020

Streszczenie:
Micro- and nanofibers are historically-known materials that are continuously reinvented due to their valuable properties. They display promise for applications in many fields, from tissue engineering to catalysis or sensors. In the first application, micro- and nanofibers are mainly produced from a limited library of biomaterials with properties that need alteration before use. Post-modification is a very effective method for attaining on-demand features and functions of nonwovens. This review summarizes and presents methods of functionalization of nonwovens produced by electrostatic means. The reviewed modifications are grouped into physical methods, chemical modification, and mixed methods.

Słowa kluczowe:
nanofiber post-modification, functional nanofibers, tissue engineering

Afiliacje autorów:
Kowalczyk T. - IPPT PAN
100p.
2.  Rokicki G., Kowalczyk T., Kaczorowski M., Sześcioczłonowe węglany cykliczne jako modyfikatory żywic epoksydowych, POLIMERY, ISSN: 0032-2725, DOI: 10.14314/polimery.2018.2.2, Vol.63, No.2, pp.90-101, 2018

Streszczenie:
Przedstawiono metodę modyfikacji żywic epoksydowych biscyklicznymi węglanami sześcioczłonowymi. Wykazano, że katalizowana kwasami Lewisa reakcja oksiranów z sześcioczłonowymi węglanami cyklicznymi przebiega głównie z wytworzeniem spiroortowęglanu pięcio-sześcioczłonowego. W jej wyniku powstają poli(etero-węglany), polietery i pięcioczłonowe węglany cykliczne. W niskiej temperaturze reakcja może ulec zahamowaniu na etapie spiroortowęglanu. Wykazano, że żywice epoksydowe zmodyfikowane dodatkiem 10 % mas. oligomeru uretanowego, zakończonego sześcioczłonowymi cyklicznymi węglanami lub biscyklicznym węglanem zawierającym resztę glikolu tri(oksyetylenowego), charakteryzują się udarnością o 66–83 % większą niż żywica niemodyfikowana, przy zachowanym poziomie wartości pozostałych cech wytrzymałościowych.

Słowa kluczowe:
biscykliczne węglany sześcioczłonowe, modyfikacja żywicy epoksydowej, cykliczny węglan jako aktywny rozcieńczalnik, kationowa kopolimeryzacja oksiranów z sześcioczłonowymi cyklicznymi węglanami, polimeryzacja z otwarciem pierścienia

Afiliacje autorów:
Rokicki G. - Warsaw University of Technology (PL)
Kowalczyk T. - IPPT PAN
Kaczorowski M. - Warsaw University of Technology (PL)
15p.
3.  Jundziłł A., Pokrywczyńska M., Adamowicz J., Kowalczyk T., Nowacki M., Bodnar M., Marszałek A., Frontczak-Baniewicz M.M., Mikułowski G., Kloskowski T., Gatherwright J., Drewa T., Vascularization Potential of Electrospun Poly(L-Lactide-co-Caprolactone) Scaffold: The Impact for Tissue Engineering, Medical Science Monitor, ISSN: 1643-3750, DOI: 10.12659/MSM.899659, Vol.23, pp.1540-1551, 2017

Streszczenie:
BACKGROUND: Electrospun nanofibers have widespread putative applications in the field of regenerative medicine and tissue engineering. When compared to naturally occurring collagen matrices, electrospun nanofiber scaffolds have two distinct advantages: they do not induce a foreign body reaction and they are not at risk for biological contamination. However, the exact substrate, structure, and production methods have yet to be defined. MATERIAL AND METHODS: In the current study, tubular-shaped poly(L-lactide-co-caprolactone) (PLCL) constructs produced using electrospinning technology were evaluated for their potential application in the field of tissue regeneration in two separate anatomic locations: the skin and the abdomen. The constructs were designed to have an internal diameter of 3 mm and thickness of 200 μm. Using a rodent model, 20 PLCL tubular constructs were surgically implanted in the abdominal cavity and subcutaneously. The constructs were then evaluated histologically using electron microscopy at 6 weeks post-implantation. RESULTS: Histological evaluation and analysis using scanning electron microscopy showed that pure scaffolds by themselves were able to induce angiogenesis after implantation in the rat model. Vascularization was observed in both tested groups; however, better results were obtained after intraperitoneal implantation. Formation of more and larger vessels that migrated inside the scaffold was observed after implantation into the peritoneum. In this group no evidence of inflammation and better integration of scaffold with host tissue were noticed. Subcutaneous implantation resulted in more fibrotic reaction, and differences in cell morphology were also observed between the two tested groups. CONCLUSIONS: This study provides a standardized evaluation of a PLCL conduit structure in two different anatomic locations, demonstrating the excellent ability of the structure to achieve vascularization. Functional, histological, and mechanical data clearly indicate prospective clinical utilization of PLCL in critical size defect regeneration.

Słowa kluczowe:
Polymers, Regenerative medicine, Tissue Engineering, Tissue Scaffolds, Urinary Diversion

Afiliacje autorów:
Jundziłł A. - inna afiliacja
Pokrywczyńska M. - inna afiliacja
Adamowicz J. - Nicolaus Copernicus University (PL)
Kowalczyk T. - IPPT PAN
Nowacki M. - inna afiliacja
Bodnar M. - Nicolaus Copernicus University (PL)
Marszałek A. - Nicolaus Copernicus University (PL)
Frontczak-Baniewicz M.M. - Mossakowski Medical Research Centre, Polish Academy of Sciences (PL)
Mikułowski G. - IPPT PAN
Kloskowski T. - inna afiliacja
Gatherwright J. - University Hospitals – Case Medical Center (US)
Drewa T. - Nicolaus Copernicus University (PL)
20p.
4.  Adamowicz J., Pokrywczyńska M., Tworkiewicz J., Kowalczyk T., van Breda S.V., Tyloch D., Kloskowski T., Bodnar M., Skopińska-Wiśniewska J., Marszałek A., Frontczak-Baniewicz M.M., Kowalewski T.A., Drewa T., New Amniotic Membrane Based Biocomposite for Future Application in Reconstructive Urology, PLOS ONE, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0146012, Vol.11, No.1, pp.e0146012-1-20, 2016

Streszczenie:
Objective
Due to the capacity of the amniotic membrane (Am) to support re-epithelisation and inhibit scar formation, Am has a potential to become a considerable asset for reconstructive urology i.e., reconstruction of ureters and urethrae. The application of Am in reconstructive urology is limited due to a poor mechanical characteristic. Am reinforcement with electrospun nanofibers offers a new strategy to improve Am mechanical resistance, without affecting its unique bioactivity profile. This study evaluated biocomposite material composed of Am and nanofibers as a graft for urinary bladder augmentation in a rat model.

Material and Methods
Sandwich-structured biocomposite material was constructed from frozen Am and covered on both sides with two-layered membranes prepared from electrospun poly-(L-lactide-co-E-caprolactone) (PLCL). Wistar rats underwent hemicystectomy and bladder augmentation with the biocomposite material.

Results
Immunohistohemical analysis (hematoxylin and eosin [H&E], anti-smoothelin and Masson’s trichrome staining [TRI]) revealed effective regeneration of the urothelial and smooth muscle layers. Anti-smoothelin staining confirmed the presence of contractile smooth muscle within a new bladder wall. Sandwich-structured biocomposite graft material was designed to regenerate the urinary bladder wall, fulfilling the requirements for normal bladder tension, contraction, elasticity and compliance. Mechanical evaluation of regenerated bladder wall conducted based on Young’s elastic modulus reflected changes in the histological remodeling of the augmented part of the bladder. The structure of the biocomposite material made it possible to deliver an intact Am to the area for regeneration. An unmodified Am surface supported regeneration of the urinary bladder wall and the PLCL membranes did not disturb the regeneration process.

Conclusions
Am reinforcement with electrospun nanofibers offers a new strategy to improve Am mechanical resistance without affecting its unique bioactivity profile.

Słowa kluczowe:
Bladder, Smooth muscles, Muscle regeneration, Bionanotechnology, Renal system, Urothelium, Urology, Nanomaterials

Afiliacje autorów:
Adamowicz J. - Nicolaus Copernicus University (PL)
Pokrywczyńska M. - inna afiliacja
Tworkiewicz J. - inna afiliacja
Kowalczyk T. - IPPT PAN
van Breda S.V. - University of Pretoria (ZA)
Tyloch D. - inna afiliacja
Kloskowski T. - inna afiliacja
Bodnar M. - Nicolaus Copernicus University (PL)
Skopińska-Wiśniewska J. - inna afiliacja
Marszałek A. - Nicolaus Copernicus University (PL)
Frontczak-Baniewicz M.M. - Mossakowski Medical Research Centre, Polish Academy of Sciences (PL)
Kowalewski T.A. - IPPT PAN
Drewa T. - Nicolaus Copernicus University (PL)
35p.
5.  Noszczyk B.H., Kowalczyk T., Łyżniak M., Zembrzycki K., Mikułowski G., Wysocki J., Kawiak J., Pojda Z., Biocompatibility of electrospun human albumin: a pilot study, Biofabrication, ISSN: 1758-5082, DOI: 10.1088/1758-5090/7/1/015011, Vol.7, pp.015011-1-11, 2015

Streszczenie:
Albumin is rarely used for electrospinning because it does not form fibres in its native globular form. This paper presents a novel method for electrospinning human albumin from a solution containing pharmaceutical grade protein and 25% polyethylene oxide (PEO) used as the fibre-forming agent. After spontaneous cross-linking at body temperature, with no further chemicals added, the fibres become insoluble and the excess PEO can be washed out. Albumin deposited along the fibres retains its native characteristics, such as its non-adhesiveness to cells and its susceptibility for degradation by macrophages. To demonstrate this we evaluated the mechanical properties, biocompatibility and biodegradability of this novel product. After subcutaneous implantation in mice, albumin mats were completely resorbable within six days and elicited only a limited local inflammatory response. In vitro, the mats suppressed cell attachment and migration. As this product is inexpensive, produced from human pharmaceutical grade albumin without chemical modifications, retains its native protein properties and fulfils the specific requirements for anti-adhesive dressings, its clinical use can be expedited. We believe that it could specifically be used when treating paediatric patients with epidermolysis bullosa, in whom non-healing wounds occur after minor hand injuries which lead to rapid adhesions and devastating contractures.

Słowa kluczowe:
albumin, nanofibers, wound dressing, biocompatibility, bioresorption

Afiliacje autorów:
Noszczyk B.H. - Medical University of Warsaw (PL)
Kowalczyk T. - IPPT PAN
Łyżniak M. - Central Clinical Hospital of the MSWiA (PL)
Zembrzycki K. - IPPT PAN
Mikułowski G. - IPPT PAN
Wysocki J. - Institute of Oncology (PL)
Kawiak J. - Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)
Pojda Z. - Institute of Oncology (PL)
45p.
6.  Nakielski P., Kowalczyk T., Zembrzycki K., Kowalewski T.A., Experimental and numerical evaluation of drug release from nanofiber mats to brain tissue, Journal of Biomedical Materials Research Part B: Applied Biomaterials, ISSN: 1552-4973, DOI: 10.1002/jbm.b.33197, Vol.103B, No.2, pp.282-291, 2015

Streszczenie:
Drug delivery systems based on nanofibrous mats appear to be a promising healing practice for preventing brain neurodegeneration after surgery. One of the problems encountered during planning and constructing optimal delivery system based on nanofibrous mats is the estimation of parameters crucial for predicting drug release dynamics. This study describes our experimental setup allowing for spatial and temporary evaluation of drug release from nanofibrous polymers to obtain data necessary to validate appropriate numerical models. We applied laser light sheet method to illuminate released fluorescent drug analog and CCD camera for imaging selected cross-section of the investigated volume. Transparent hydrogel was used as a brain tissue phantom. The proposed setup allows for continuous observation of drug analog (fluorescent dye) diffusion for time span of several weeks. Images captured at selected time intervals were processed to determine concentration profiles and drug release kinetics. We used presented method to evaluate drug release from several polymers to validate numerical model used for optimizing nanofiber system for neuroprotective dressing.

Słowa kluczowe:
neural therapy, brain phantom, drug delivery, laser light sheet, computational modeling, nanofibers

Afiliacje autorów:
Nakielski P. - IPPT PAN
Kowalczyk T. - IPPT PAN
Zembrzycki K. - IPPT PAN
Kowalewski T.A. - IPPT PAN
30p.
7.  Kloskowski T., Jundziłł A., Kowalczyk T., Nowacki M., Bodnar M., Marszałek A., Pokrywczyńska M., Frontczak-Baniewicz M.M., Kowalewski T.A., Chłosta P., Drewa T., Ureter Regeneration–The Proper Scaffold Has to Be Defined, PLOS ONE, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0106023, Vol.9, No.8, pp.106023-1-13, 2014

Streszczenie:
The aim of this study was to compare two different acellular scaffolds: natural and synthetic, for urinary conduit construction and ureter segment reconstruction. Acellular aortic arch (AAM) and poly(L-lactide-co-caprolactone) (PLCL) were used in 24 rats for ureter reconstruction in both tested groups. Follow-up period was 4 weeks. Intravenous pyelography, histological and immunohistochemical analysis were performed. All animals survived surgical procedures. Patent uretero-conduit junction was observed only in one case using PLCL. In case of ureter segment reconstruction ureters were patent in one case using AAM and in four cases using PLCL scaffolds. Regeneration of urothelium layer and focal regeneration of smooth muscle layer was observed on both tested scaffolds. Obtained results indicates that synthetic acellular PLCL scaffolds showed better properties for ureter reconstruction than naturally derived acellular aortic arch.

Słowa kluczowe:
Ureter, Muscle regeneration, Kidneys, Collagens, Urine, Surgical and invasive medical procedures, Smooth muscles, Inflammation

Afiliacje autorów:
Kloskowski T. - inna afiliacja
Jundziłł A. - inna afiliacja
Kowalczyk T. - IPPT PAN
Nowacki M. - inna afiliacja
Bodnar M. - Nicolaus Copernicus University (PL)
Marszałek A. - Nicolaus Copernicus University (PL)
Pokrywczyńska M. - inna afiliacja
Frontczak-Baniewicz M.M. - Mossakowski Medical Research Centre, Polish Academy of Sciences (PL)
Kowalewski T.A. - IPPT PAN
Chłosta P. - Jagiellonian University (PL)
Drewa T. - Nicolaus Copernicus University (PL)
40p.
8.  Pokrywczyńska M., Jundziłł A., Adamowicz J., Kowalczyk T., Warda K., Rasmus M., Buchholz Ł., Krzyżanowska S., Nakielski P., Chmielewski T., Bodnar M., Marszałek A., Dębski R., Frontczak-Baniewicz M.M., Mikułowski G., Nowacki M., Kowalewski T.A., Drewa T., Is the Poly (L- Lactide- Co– Caprolactone) Nanofibrous Membrane Suitable for Urinary Bladder Regeneration?, PLOS ONE, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0105295, Vol.9, No.8, pp.105295-1-12, 2014

Streszczenie:
The purpose of this study was to compare: a new five-layered poly (L–lactide–co–caprolactone) (PLC) membrane and small intestinal submucosa (SIS) as a control in rat urinary bladder wall regeneration. The five-layered poly (L–lactide–co–caprolactone) membrane was prepared by an electrospinning process. Adipose tissue was harvested from five 8-week old male Wistar rats. Adipose derived stem cells (ADSCs) were seeded in a density of 3×106 cells/cm2 onto PLC membrane and SIS scaffolds, and cultured for 5-7 days in the stem cell culture medium. Twenty male Wistar rats were randomly divided into five equal groups. Augmentation cystoplasty was performed in a previously created dome defect. Groups: (I) PLC+ 3×106ADSCs; (II) SIS+ 3×106ADSCs; (III) PLC; (IV) SIS; (V) control. Cystography was performed after three months. The reconstructed urinary bladders were evaluated in H&E and Masson's trichrome staining. Regeneration of all components of the normal urinary bladder wall was observed in bladders augmented with cell-seeded SIS matrices. The urinary bladders augmented with SIS matrices without cells showed fibrosis and graft contraction. Bladder augmentation with the PLC membrane led to numerous undesirable events including: bladder wall perforation, fistula or diverticula formation, and incorporation of the reconstructed wall into the bladder lumen. The new five-layered poly (L–lactide–co–caprolactone) membrane possesses poorer potential for regenerating the urinary bladder wall compared with SIS scaffold.

Słowa kluczowe:
urinary bladder regeneration, electrospinning

Afiliacje autorów:
Pokrywczyńska M. - inna afiliacja
Jundziłł A. - inna afiliacja
Adamowicz J. - Nicolaus Copernicus University (PL)
Kowalczyk T. - IPPT PAN
Warda K. - inna afiliacja
Rasmus M. - Nicolaus Copernicus University (PL)
Buchholz Ł. - Nicolaus Copernicus University (PL)
Krzyżanowska S. - inna afiliacja
Nakielski P. - IPPT PAN
Chmielewski T. - IPPT PAN
Bodnar M. - Nicolaus Copernicus University (PL)
Marszałek A. - Nicolaus Copernicus University (PL)
Dębski R. - Nicolaus Copernicus University (PL)
Frontczak-Baniewicz M.M. - Mossakowski Medical Research Centre, Polish Academy of Sciences (PL)
Mikułowski G. - IPPT PAN
Nowacki M. - inna afiliacja
Kowalewski T.A. - IPPT PAN
Drewa T. - Nicolaus Copernicus University (PL)
40p.
9.  Gomez-Sanchez C., Kowalczyk T., Ruiz De Eguino G., Lopez-Arraiza A., Infante A., Rodriguez C.I., Kowalewski T.A., Sarrionandia M., Aurrekoetxea J., Electrospinning of poly(lactic acid)/polyhedral oligomeric silsesquioxane nanocomposites and their potential in chondrogenic tissue regeneration, JOURNAL OF BIOMATERIALS SCIENCE, POLYMER EDITION, ISSN: 0920-5063, DOI: 10.1080/09205063.2014.910151, Vol.25, No.8, pp.802-825, 2014

Streszczenie:
The study was conducted to evaluate the cytocompatibility and hydrolytic degradability of the new poly(lactic acid)/polyethylene glycol-polyhedral oligomeric silsesquioxane (peg-POSS/PLLA) nanocomposite as potential material for cartilage regeneration. PLLA scaffolds containing 0 to 5% of peg-POSS were fabricated by electrospinning. Human mesenchymal stem cells (hMSC’s) were cultured in vitro to evaluate the cytocompatibility of the new nanocomposite material. Hydrolytic degradation studies were also carried out to analyze the mass loss rate of the nanocomposites through time. The addition of the peg-POSS to the PLLA did not affect the processability of the nanocomposite by electrospinning. It was also observed that peg-POSS did not show any relevant change in fibers morphology, concluding that it was well dispersed. However, addition of peg-POSS caused noticeable decrease in mean fiber diameter, which made the specific surface area of the scaffold to rise. hMSC’s were able to attach, to proliferate, and to differentiate into chondrocytes in a similar way onto the different types of electrospun peg-POSS/PLLA and pure PLLA scaffolds, showing that the peg-POSS as nano-additive does not exhibit any cytotoxicity. The hydrolytic degradation rate of the material was lower when peg-POSS was added, showing a higher durability of the nanocomposites through time. Results demonstrate that the addition of peg-POSS to the PLLA scaffolds does not affect its cytocompatibility to obtain hyaline cartilage from hMSC’s.

Słowa kluczowe:
PLLA, peg-POSS, nanocomposite, electrospinning, chondrocyte, stem cells, scaffold, cartilage repair, hydrolytic degradation

Afiliacje autorów:
Gomez-Sanchez C. - Mondragon Unibertsitatea (ES)
Kowalczyk T. - IPPT PAN
Ruiz De Eguino G. - Hospital Universitario Cruces (ES)
Lopez-Arraiza A. - University of the Basque Country (ES)
Infante A. - Hospital Universitario Cruces (ES)
Rodriguez C.I. - Hospital Universitario Cruces (ES)
Kowalewski T.A. - IPPT PAN
Sarrionandia M. - Mondragon Unibertsitatea (ES)
Aurrekoetxea J. - Mondragon Unibertsitatea (ES)
25p.
10.  Sulejczak D., Andrychowski J., Kowalczyk T., Nakielski P., Frontczak-Baniewicz M.M., Kowalewski T.A., Electrospun nanofiber mat as a protector against the consequences of brain injury, FOLIA NEUROPATHOLOGICA, ISSN: 1641-4640, DOI: 10.5114/fn.2014.41744, Vol.52, No.1, pp.56-69, 2014

Streszczenie:
Traumatic/surgical brain injury can initiate a cascade of pathological changes that result, in the long run, in severe damage of brain parenchyma and encephalopathy. Excessive scarring can also interfere with brain function and the glial scar formed may hamper the restoration of damaged brain neural pathways. In this preliminary study we aimed to investigate the effect of dressing with an L-lactide-caprolactone copolymer nanofiber net on brain wound healing and the fate of the formed glial scar. Our rat model of surgical brain injury (SBI) of the fronto-temporal region of the sensorimotor cortex imitates well the respective human neurosurgery situation. Brains derived from SBI rats with net-undressed wound showed massive neurodegeneration, entry of systemic inflammatory cells into the brain parenchyma and the astrogliosis due to massive glial scar formation. Dressing of the wound with the nanofiber net delayed and reduced the destructive phenomena. We observed also a reduction in the scar thickness. The observed modification of local inflammation and cicatrization suggest that nanofiber nets could be useful in human neurosurgery.

Słowa kluczowe:
brain injury, L-lactide-caprolactone copolymer nanofiber net, glial scar, neurodegeneration

Afiliacje autorów:
Sulejczak D. - inna afiliacja
Andrychowski J. - Medical University of Warsaw (PL)
Kowalczyk T. - IPPT PAN
Nakielski P. - IPPT PAN
Frontczak-Baniewicz M.M. - Mossakowski Medical Research Centre, Polish Academy of Sciences (PL)
Kowalewski T.A. - IPPT PAN
20p.
11.  Kloskowski T., Kowalczyk T., Nowacki M., Drewa T., Tissue engineering and ureter regeneration: Is it possible?, INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS, ISSN: 0391-3988, DOI: 10.5301/ijao.5000130, Vol.36, No.6, pp.392-405, 2013

Streszczenie:
Large ureter damages are difficult to reconstruct. Current techniques are complicated, difficult to perform, and often associated with failures. The ureter has never been regenerated thus far. Therefore the use of tissue engineering techniques for ureter reconstruction and regeneration seems to be a promising way to resolve these problems. For proper ureter regeneration the following problems must be considered: the physiological aspects of the tissue, the type and shape of the scaffold, the type of cells, and the specific environment (urine). 
This review presents tissue engineering achievements in the field of ureter regeneration focusing on the scaffold, the cells, and ureter healing.

Afiliacje autorów:
Kloskowski T. - inna afiliacja
Kowalczyk T. - IPPT PAN
Nowacki M. - inna afiliacja
Drewa T. - Nicolaus Copernicus University (PL)
20p.
12.  Andrychowski J., Frontczak-Baniewicz M.M., Sulejczak D., Kowalczyk T., Chmielewski T., Czernicki Z., Kowalewski T.A., Nanofiber nets in prevention of cicatrisation in spinal procedures. Experimental study, FOLIA NEUROPATHOLOGICA, ISSN: 1641-4640, DOI: 10.5114/fn.2013.35958, Vol.51, No.2, pp.147-157, 2013

Streszczenie:
Excessive cicatrisation or epidural fibrosis in the operative field is an inappropriate event occasionally occurring after neurosurgical procedures (i.e., spine procedures and craniotomies). This excessive process may disturb the postopera­tive course and render reoperations more difficult and risky. The literature describes this phenomenon as accompanying up to 20% of neurosurgical procedures. The scar tissue that forms postoperatively adheres to the dura mater, penetrates into the spinal canal and can cause narrowing symptoms, neurological deficits and pain. The incidence and spread of this excessive scar or epidural fibrosis can be prevented through the modification of the surgical technique by incorporating endoscopic or microscopic access to minimize the operative field and the use of isolating substances (autogenous or heterogeneous) administered intraoperatively.

The aim of this experimental study was to morphologically assess the cicatrisation process, adhesion and to prevent excessive scar formation with the local use of membranes manufactured by an electrospinning process (nanotechnology). We also investigated whether the biodegradable nanofibrous net triggers or modifies the immunological response or the local inflammatory process.

Micro-nanofibrous membranes were produced by the electrospinning process. A biodegradable, medically certified copolymer poly(L-lactide-co-caprolactone) (PLCL) was used as the electrospun material.

An experimental rat model was used in this study. Experimental and control groups were formed with specified follow-up times of 4, 14 and 30 days. During the operation, a two-level laminectomy in the thoracic segment was performed. The operative field was divided into two regions. Isolating material was used on the dura mater and surface of the spinal cord in the area where the laminectomy was performed. The material was analysed with the use of light and electron microscopy.

Local cicatrisation can be modified using nanomaterials. Scar formation and epidural fibrosis can be limited and modi­fied locally. No local inflammation process was observed.

Initial observations indicate the potential for the effective use of materials obtained in the electrospinning process to prevent cicatrisation.

Słowa kluczowe:
neurosurgery, cicatrisation, epidural fibrosis, copolymer, poly(L-lactide-co-caprolactone), nanofibrous net, rat model

Afiliacje autorów:
Andrychowski J. - Medical University of Warsaw (PL)
Frontczak-Baniewicz M.M. - Mossakowski Medical Research Centre, Polish Academy of Sciences (PL)
Sulejczak D. - inna afiliacja
Kowalczyk T. - IPPT PAN
Chmielewski T. - IPPT PAN
Czernicki Z. - Mossakowski Medical Research Centre, Polish Academy of Sciences (PL)
Kowalewski T.A. - IPPT PAN
15p.
13.  Nakielski P., Kowalczyk T., Kowalewski T.A., Drug delivery system based on polymer nano-fibers, Prace IPPT - IFTR Reports, ISSN: 2299-3657, Vol.4b, pp.1-6, 2013

Streszczenie:
This work presents our attempts to characterize release of two model drugs from electrospun polymer nanofibers. Such drug delivery system offers great potential for applications in medicine especially as neurosurgery protective membranes. Proper delivery of drugs requires precise control of the drug diffusion process during the release for days or even weeks. Lipophilic model drug Rhodamine B and hydrophilic Bovine Serum Albumin conjugated with Fluoresceine (BSA - FITC) were embedded in electrospun poly(L -lactide-co -ε-caprolactone) (PLC) nanofibers. Release of Rhodamine B showed saturation in cumulative release profile at 60% and 86% for 1.5% and 3% wt. initial drug content, respectively. Nanofibers electrospun from emulsion released almost entire drug encapsulated in water vesicles inside the nanofibers. Possible location of vesicles close to the surface of the nanofibers exposed them for surrounding fluid and caused leaching of the drug. In this case encapsulation of drug in emulsion prevented the initial burst release.Dependence of a drug release and composition of nanofiber is essential for production of drug delivery systems. Mathematical model constructed with this data allows to avoid tedious experimental work. This research was supported by Ministry of Science and Higher Education, National Centre for Research and Development Project grant no. R13008110. The first author has been supported with a scholarship from the European Social Fund, Human Capital Operational Programme.

Słowa kluczowe:
nanofibres, drug delivery, vesicles

Afiliacje autorów:
Nakielski P. - IPPT PAN
Kowalczyk T. - IPPT PAN
Kowalewski T.A. - IPPT PAN
14.  Adamowicz J., Kowalczyk T., Drewa T., Tissue engineering of urinary bladder – current state of art and future perspectives, Central European Journal of Urology, ISSN: 2080-4806, DOI: 10.5173/ceju.2013.02.art23, Vol.66, pp.202-206, 2013

Streszczenie:
Introduction.
Tissue engineering and biomaterials science currently offer the technology needed to replace the urinary tract wall. This review addresses current achievements and barriers for the regeneration of the urinary bladder based on tissue engineering methods. Materials and methods. Medline was search for urinary bladder tissue engineering regenerative medicine and stem cells.

Results.
Numerous studies to develop a substitute for the native urinary bladder wall using the tissue engineering approach are ongoing. Stem cells combined with biomaterials open new treatment methods, including even de novo urinary bladder construction. However, there are still many issues before advances in tissue engineering can be introduced for clinical application.

Conclusions.
Before tissue engineering techniques could be recognize as effective and safe for patients, more research studies performed on large animal models and with long follow–up are needed to carry on in the future.

Słowa kluczowe:
stem cells, bladder regeneration, tissue engineering

Afiliacje autorów:
Adamowicz J. - Nicolaus Copernicus University (PL)
Kowalczyk T. - IPPT PAN
Drewa T. - Nicolaus Copernicus University (PL)
15.  Blim A., Kowalczyk T., Dynamic lattice liquid (DLL) model in computer simulation of the structure and dynamics of polymer condensed systems, E-POLYMERS, ISSN: 1618-7229, Vol.2012, No.079, pp.1-11, 201220p.
16.  Nowacki M., Jundziłł A., Bieniek M., Kowalczyk T., Kloskowski T., Drewa T., Nowoczesne biomateriały jako opatrunki hemostatyczne w chirurgii oszczędzającej miąższ nerki-model zwierzęcy. Doniesienie wstępne., POLIMERY W MEDYCYNIE, ISSN: 0370-0747, Vol.42, No.1, pp.35-43, 20125p.
17.  Kowalewski T.A., Kowalczyk T., Frontczak-Baniewicz M.M., Gołąbek-Sulejczak D.A., Andrychowski J., Nanofibres for medical applications at Biocentrum Ochota, Annual Report - Polish Academy of Sciences, ISSN: 1640-3754, pp.62-65, 2011

Streszczenie:
Electrospun nonwovens have recently been successfully applied as a dressing material in spinal neurosurgery. Scarring is known to be one of the major post-operative complications for neurosurgery. If it occurs, it may trap a nerve, so that when a patient moves the nerve becomes stretched, causing nerve damage, pain, and internal scarring of the nerve. This causes subsequent complications related to ingrowths of connective tissue onto the spinal canal. The formation of an astroglial scar is another serious postoperative complication of brain neuro-surgery. The use of bio-absorbable isolative materials as anti-liaison protection and as possible carriers for neuroprotective drug delivery is expected to help in solving such problems. The nanostructured material acts as an anti-bacterial and anti-liaison barrier while enabling transport of oxygen, nutrients, and metabolites, facilitating the healing process of the surgical wound.

Słowa kluczowe:
Nanofibres for neurosurgery, protection of scar formation

Afiliacje autorów:
Kowalewski T.A. - IPPT PAN
Kowalczyk T. - IPPT PAN
Frontczak-Baniewicz M.M. - Mossakowski Medical Research Centre, Polish Academy of Sciences (PL)
Gołąbek-Sulejczak D.A. - inna afiliacja
Andrychowski J. - Medical University of Warsaw (PL)
18.  Fryczkowski R., Kowalczyk T., Nanofibres from polyaniline/polyhydroxybutyrate blends, SYNTHETIC METALS, ISSN: 0379-6779, Vol.159, No.21-22, pp.2266-2268, 200932p.
19.  Bretcanu O., Misra S.K., Yunos D.M., Boccaccini A.R., Roy I., Kowalczyk T., Błoński S., Kowalewski T.A., Electrospun nanofibrous biodegradable polyester coatings on Bioglass®-based glass-ceramics for tissue engineering, MATERIALS CHEMISTRY AND PHYSICS, ISSN: 0254-0584, DOI: 10.1016/j.matchemphys.2009.08.011, Vol.118, pp.420-426, 2009

Streszczenie:
Biodegradable polymeric nanofibrous coatings were obtained by electrospinning different polymers onto sintered 45S5 Bioglass®-based glass-ceramic pellets. The investigated polymers were poly(3-hydroxybutyrate) (P3HB), poly(3-hydroxybutyrate-co-hydroxyvalerate) (PHBV) and a composite of poly(caprolactone) (PCL) and poly(ethylene oxide) (PEO) (PCL–PEO). The fibrous coatings morphology was evaluated by optical microscopy and scanning electron microscopy. The electrospinning process parameters were optimised to obtain reproducible coatings formed by a thin web of polymer nanofibres. In-vitro studies in simulated body fluid (SBF) were performed to investigate the bioactivity and mineralisation of the substrates by inducing the formation of hydroxyapatite (HA) on the nanofiber-coated pellets. HA crystals were detected on all samples after 7 days of immersion in SBF, however the morphology of the HA layer depended on the characteristic fibre diameter, which in turn was a function of the specific polymer-solvent system used. The bioactive and resorbable nanofibrous coatings can be used to tailor the surface topography of bioactive glass-ceramics for applications in tissue engineering scaffolds.

Słowa kluczowe:
Electrospinning, Nanofibers, Bioglass®, Polyhydroxyalkanoates, Tissue engineering

Afiliacje autorów:
Bretcanu O. - inna afiliacja
Misra S.K. - inna afiliacja
Yunos D.M. - inna afiliacja
Boccaccini A.R. - Friedrich-Alexander University of Erlangen-Nürnberg (DE)
Roy I. - inna afiliacja
Kowalczyk T. - IPPT PAN
Błoński S. - IPPT PAN
Kowalewski T.A. - IPPT PAN
32p.
20.  Kowalczyk T., Nowicka A., Elbaum D., Kowalewski T.A., Electrospinning of bovine serum albumin. Optimization and the use for production of biosensors, BIOMACROMOLECULES, ISSN: 1525-7797, DOI: 10.1021/bm800421s, Vol.9, No.7, pp.2087-2090, 2008

Streszczenie:
Electrospinning of the globular protein, bovine serum albumin (BSA), was optimized to obtain proteinous fibers suitable asbiosensors. It was shown that the as-spun protein preserves its native form, whereas solubility of the cross-linked in the ambient conditions BSA nanofibers evidently decreases. Insoluble BSA fibers can be easily modified to be used as two-dimensional biosensors. Here, we show the micro pH sensor obtained from the BSA fiber stained with a fluorescein derivative (FITC).

Słowa kluczowe:
Electrospinning BSA, pH sensor

Afiliacje autorów:
Kowalczyk T. - IPPT PAN
Nowicka A. - IPPT PAN
Elbaum D. - inna afiliacja
Kowalewski T.A. - IPPT PAN

Lista rozdziałów w ostatnich monografiach
1. 
Gambin B., Lekszycki T., Kowalczyk T., Kowalewski T.A., Ziółkowski A., Pieczyska E.A., Stupkiewicz S., Scenariusze rozwoju technologii nowoczesnych materiałów metalicznych, ceramicznych i kompozytowych, rozdział: Biomateriały. Rola i strategia badawcza IPPT PAN w powstaniu nowoczesnych technologii materiałów współpracujących z ludzkimi tkankami, Wydawnictwo Naukowe Instytutu Technologii Eksploatacji PIB, 1, pp.181-222, 2010
2. 
Kowalewski T.A., Barral S., Kowalczyk T., IUTAM Symposium on modelling nanomaterials and nanosystems, IUTAM Bookseries, rozdział: Modeling electrospinning of nanofibers, Springer, Pyrz R., Rauhe J.C. (Eds.), 13, pp.279-292, 2009

Prace konferencyjne
1.  Nakielski P., Kowalczyk T., Kowalewski T.A., Modeling Drug Release from Materials Based on Electrospun Nanofibers, COMSOL 2013, COMSOL Conference, 2013-10-23/10-25, Rotterdam (NL), pp.1-6, 2013

Streszczenie:
Comprehensive studies of drug transport in nanofibres based mats have been performed to predict drug release kinetics. The paper presents our approach to analyze the impact of fibers arrangement, one of the parameters varied in our parallel experimental studies. Drug encapsulation in submicron fibers and subsequent controlled release of drugs is a tedious task due to the large number of process and material parameters involved. In the numerical study we constructed a 3D finite element geometry representing nanofibrous cubic element. COMSOL Multiphysics has been used to assess the impact of the various purposed arrangements of fibers within the mat. Drug release from nanofibers was modeled by adsorption -desorption and diffusion equation, where drug diffusion coefficient in the fluid between the fibers was altered depending on porosity of the material. Our study shows that for the same material porosity drug release from the matrix of regularly oriented fibers is slower than from randomly oriented, isotropic nanofibrous material. Also by decreasing distance between the fibers drug transport rate is reduced.

Słowa kluczowe:
Nanofibres, finite elements, drug release modelling

Afiliacje autorów:
Nakielski P. - IPPT PAN
Kowalczyk T. - IPPT PAN
Kowalewski T.A. - IPPT PAN
2.  Kowalewski T.A., Barral S., Kowalczyk T., Modeling Electrospinning of Nanofibers, in Modelling Nanomaterials and Nanosystems, IUTAM Symposium, 2009-05-19/05-22, Aalborg (DK), Vol.13, pp.279-292, 2009

Streszczenie:
A fast discrete model for the simulations of thin charged jets produced during the electrospinning process is derived, based on an efficient implementation of the boundary element method for the computation of electrostatic interactions of the jet with itself and with the electrodes. Short-range electrostatic forces are evaluated with slender-body analytical approximations, whereas a hierarchical force evaluation algorithm is used for long-range interactions. Qualitative comparisons with experiments is discussed.

Słowa kluczowe:
modelling electrospinning, electrostatic interactions, BEM

Afiliacje autorów:
Kowalewski T.A. - IPPT PAN
Barral S. - IPPT PAN
Kowalczyk T. - IPPT PAN

Abstrakty konferencyjne
1.  Kowalczyk T., Niemczyk B., Kloskowski T., Jundziłł A., Adamowicz J., Nowacki M., Pokrywczyńska M., Noszczyk B., Drewa T., Investigation of the in vivo behavior of membranes made of electrospun micro and nanofibers implanted on an animal model, CNM 2019, 6th CONFERENCE ON NANO- AND MICROMECHANICS, 2019-07-03/07-05, Rzeszów (PL), pp.137-139, 2019

Słowa kluczowe:
electrospinning, microfibers, nanofibers, in vivo, animal model

Afiliacje autorów:
Kowalczyk T. - IPPT PAN
Niemczyk B. - IPPT PAN
Kloskowski T. - inna afiliacja
Jundziłł A. - inna afiliacja
Adamowicz J. - Nicolaus Copernicus University (PL)
Nowacki M. - inna afiliacja
Pokrywczyńska M. - inna afiliacja
Noszczyk B. - Medical University of Warsaw (PL)
Drewa T. - Nicolaus Copernicus University (PL)
2.  Nakielski P., Kowalczyk T., Kowalewski T.A., Zastosowanie materiałów z nanowłókien w inżynierii regeneracyjnej, I Konferencja Młodych Naukowców „Biotechnologia w produkcji zwierzęcej” SGGW, 2014-04-24/04-25, Warszawa (PL), pp.25, 2014

Słowa kluczowe:
elektroprzędzenie, nanowłókna, uwalnianie leków, TBI

Afiliacje autorów:
Nakielski P. - IPPT PAN
Kowalczyk T. - IPPT PAN
Kowalewski T.A. - IPPT PAN
3.  Nakielski P., Kowalczyk T., Kowalewski T.A., Modeling drug delivery from nanofibers to brain tissue, XXI FMC, XXI Fluid Mechanics Conference, 2014-06-15/06-18, Kraków (PL), pp.24, 2014

Słowa kluczowe:
brain, drug delivery, nanofibres, mathematical modelling

Afiliacje autorów:
Nakielski P. - IPPT PAN
Kowalczyk T. - IPPT PAN
Kowalewski T.A. - IPPT PAN

Patenty
Numer/data zgłoszenia patentowego
Ogłoszenie o zgłoszeniu patentowym
Twórcy
Tytuł
Kraj i Nazwa uprawnionego z patentu
Numer patentu
Ogłoszenie o udzieleniu patentu
pdf
411421
2015-02-27
BUP 18/2016
2016-08-29
Kowalczyk T., Noszczyk B.H.
Sposób wytwarzania elektroprzędzonych materiałów zawierających natywne białko albuminy, mata otrzymana tym sposobem oraz jej zastosowanie, zwłaszcza jako materiałów opatrunkowych
PL, Instytut Podstawowych Problemów Techniki PAN, Centrum Medyczne Kształcenia Podyplomowego
238231
WUP 17/2021
2021-07-26



404667
2013-07-12
BUP 02/2015
2015-01-19
Andrychowski J., Frontczak-Baniewicz M.M., Czernicki Z.M., Gołąbek-Sulejczak D.A., Kowalczyk T., Kowalewski T.A., Nakielski P.
Zastosowanie siatki izolacyjnej z nanowłókien polimerowych wykonanej w technologii elektroprzędzenia do wytwarzania opatrunków neuroprotekcyjnych do stosowania w zapobieganiu pourazowym zmianom w mózgu
PL, Warszawski Uniwersytet Medyczny, Instytut Medycyny Doświadczalnej i Klinicznej PAN, Instytut Podstawowych Problemów Techniki PAN
225858
WUP 05/2017
2017-05-31



395894
2011-08-08
BUP 04/2013
2013-02-18
Andrychowski J., Frontczak-Baniewicz M.M., Czernicki Z.M., Gołąbek-Sulejczak D.A., Kowalczyk T., Kowalewski T.A.
Zastosowanie polimerowej siatki izolacyjnej z nanowłókien polimerowych do zapobiegania bliznowaceniu po zabiegu neurochirurgicznym
PL, Warszawski Uniwersytet Medyczny, Instytut Medycyny Doświadczalnej i Klinicznej PAN, Instytut Podstawowych Problemów Techniki PAN
226479
WUP 07/2017
2017-07-31



390140
2010-01-07
BUP 15/2011
2011-07-18
Kowalewski T.A., Lamparska D., Zembrzycki K., Kowalczyk T.,
Sposób wytwarzania mat z nanowłókien
PL, Instytut Podstawowych Problemów Techniki PAN
222733
WUP 08/2016
2016-08-31



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