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Imke Greving


Ostatnie publikacje
1.  Espiritu J., Sefa S., Ćwieka H., Greving I., Flenner S., Willumeit-Römer R., Seitz J.-M., Zeller-Plumhoff B., Detailing the influence of surface-treated biodegradable magnesium-based implants on the lacuno-canalicular network in sheep bone: A pilot study, Bioactive Materials, ISSN: 2452-199X, DOI: 10.2139/ssrn.4279434, pp.1-26, 2022

Streszczenie:
An increasing prevalence of bone-related injuries and aging geriatric populations continue to drive the orthopaedic implant market. A hierarchical analysis of bone remodelling after material implantation is necessary to better understand the relationship between implant and bone. Osteocytes, which are housed and communicate through the lacuno-canalicular network (LCN), are integral to bone health and remodelling processes. Therefore, it is essential to examine the framework of the LCN in response to implant materials or surface treatments.Biodegradable materials offer an alternative solution to permanent implants, which may require revision or removal surgeries. Magnesium alloys have resurfaced as promising materials due to their bone-like properties and safe degradation in vivo. To further tailor their degradation capabilities, surface treatments such as plasma electrolytic oxidation (PEO) have demonstrated to slow degradation.For the first time, the influence of a biodegradable material on the LCN is investigated by means of non-destructive 3D imaging. In this pilot study, we hypothesise noticeable variations in the LCN caused by altered chemical stimuli introduced by the PEO-coating.Utilising synchrotron-based transmission X-ray microscopy, we have characterised morphological LCN differences around uncoated and PEO-coated WE43 screws implanted into sheep bone. Bone specimens were explanted after 4, 8, and 12 weeks and regions near the implant surface were prepared for imaging. Findings from this investigation indicate that the slower degradation of PEO-coated WE43 induces healthier lacunar shapes within the LCN. However, the stimuli perceived by the uncoated material with higher degradation rates induces a greater connected LCN better prepared for bone disturbance.

Słowa kluczowe:
nanotomography, lacuno-canalicular network, Bone, magnesium, biodegradable implants

Afiliacje autorów:
Espiritu J. - inna afiliacja
Sefa S. - inna afiliacja
Ćwieka H. - IPPT PAN
Greving I. - inna afiliacja
Flenner S. - inna afiliacja
Willumeit-Römer R. - inna afiliacja
Seitz J.-M. - inna afiliacja
Zeller-Plumhoff B. - inna afiliacja
200p.
2.  Zeller-Plumhoff B., Laipple D., Słomińska H., Iskhakova K., Longo E., Hermann A., Flenner S., Greving I., Storm M., Willumeit-Romer R., Evaluating the morphology of the degradation layer of pure magnesium via 3D imaging at resolutions below 40 nm, Bioactive Materials, ISSN: 2452-199X, DOI: 10.1016/j.bioactmat.2021.04.009, Vol.6, No.12, pp.4368-4376, 2021

Streszczenie:
Magnesium is attractive for the application as a temporary bone implant due to its inherent biodegradability, non-toxicity and suitable mechanical properties. The degradation process of magnesium in physiological environments is complex and is thought to be a diffusion-limited transport problem. We use a multi-scale imaging approach using micro computed tomography and transmission X-ray microscopy (TXM) at resolutions below 40 nm. Thus, we are able to evaluate the nanoporosity of the degradation layer and infer its impact on the degradation process of pure magnesium in two physiological solutions. Magnesium samples were degraded in simulated body fluid (SBF) or Dulbecco's modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS) for one to four weeks. TXM reveals the three-dimensional interconnected pore network within the degradation layer for both solutions. The pore network morphology and degradation layer composition are similar for all samples. By contrast, the degradation layer thickness in samples degraded in SBF was significantly higher and more inhomogeneous than in DMEM+10%FBS. Distinct features could be observed within the degradation layer of samples degraded in SBF, suggesting the formation of microgalvanic cells, which are not present in samples degraded in DMEM+10%FBS. The results suggest that the nanoporosity of the degradation layer and the resulting ion diffusion processes therein have a limited influence on the overall degradation process. This indicates that the influence of organic components on the dampening of the degradation rate by the suppression of microgalvanic degradation is much greater in the present study.

Słowa kluczowe:
magnesium degradation, porosity, transmission X-ray microscopy, 3D imaging

Afiliacje autorów:
Zeller-Plumhoff B. - inna afiliacja
Laipple D. - inna afiliacja
Słomińska H. - IPPT PAN
Iskhakova K. - inna afiliacja
Longo E. - inna afiliacja
Hermann A. - inna afiliacja
Flenner S. - inna afiliacja
Greving I. - inna afiliacja
Storm M. - inna afiliacja
Willumeit-Romer R. - inna afiliacja
200p.
3.  Meyer S., Wolf A., Sanders D., Iskhakova K., Ćwieka H., Bruns S., Flenner S., Greving I., Hagemann J., Willumeit-Römer R., Wiese B., Zeller-Plumhoff B., Degradation analysis of thin Mg-xAg wires using X-ray near-field holotomography, Metals, ISSN: 2075-4701, DOI: 10.3390/met11091422, Vol.11, No.9, pp.1422-1-12, 2021

Streszczenie:
Magnesium–silver alloys are of high interest for the use as temporary bone implants due to their antibacterial properties in addition to biocompatibility and biodegradability. Thin wires in particular can be used for scaffolding, but the determination of their degradation rate and homogeneity using traditional methods is difficult. Therefore, we have employed 3D imaging using X-ray near-field holotomography with sub-micrometer resolution to study the degradation of thin (250 μm diameter) Mg-2Ag and Mg-6Ag wires. The wires were studied in two states, recrystallized and solution annealed to assess the influence of Ag content and precipitates on the degradation. Imaging was employed after degradation in Dulbecco’s modified Eagle’s medium and 10% fetal bovine serum after 1 to 7 days. At 3 days of immersion the degradation rates of both alloys in both states were similar, but at 7 days higher silver content and solution annealing lead to decreased degradation rates. The opposite was observed for the pitting factor. Overall, the standard deviation of the determined parameters was high, owing to the relatively small field of view during imaging and high degradation inhomogeneity of the samples. Nevertheless, Mg-6Ag in the solution annealed state emerges as a potential material for thin wire manufacturing for implants.

Słowa kluczowe:
X-ray computed tomography, magnesium-silver alloy, wire, degradation, near-field holotomography

Afiliacje autorów:
Meyer S. - inna afiliacja
Wolf A. - inna afiliacja
Sanders D. - inna afiliacja
Iskhakova K. - inna afiliacja
Ćwieka H. - IPPT PAN
Bruns S. - inna afiliacja
Flenner S. - inna afiliacja
Greving I. - inna afiliacja
Hagemann J. - inna afiliacja
Willumeit-Römer R. - inna afiliacja
Wiese B. - inna afiliacja
Zeller-Plumhoff B. - inna afiliacja
70p.

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