Instytut Podstawowych Problemów Techniki
Polskiej Akademii Nauk


I-Chun Lin

Ostatnie publikacje
1.  Gregory Grace E., Jones Adam P., Haley Michael J., Hoyle C., Zeef Leo A. H., Lin I., Coope David J., King Andrew T., Evans D. G., Paszek P., Couper Kevin N., Brough D., Pathmanaban Omar N., The comparable tumour microenvironment in sporadic and NF2-related schwannomatosis vestibular schwannoma, Brain Communications, ISSN: 2632-1297, DOI: 10.1093/braincomms/fcad197, Vol.5, No.4, pp.1-15, 2023

Bilateral vestibular schwannoma is the hallmark of NF2-related schwannomatosis, a rare tumour predisposition syndrome associated with a lifetime of surgical interventions, radiotherapy and off-label use of the anti-angiogenic drug bevacizumab. Unilateral vestibular schwannoma develops sporadically in non-NF2-related schwannomatosis patients for which there are no drug treatment options available. Tumour-infiltrating immune cells such as macrophages and T-cells correlate with increased vestibular schwannoma growth, which is suggested to be similar in sporadic and NF2-related schwannomatosis tumours. However, differences between NF2-related schwannomatosis and the more common sporadic disease include NF2-related schwannomatosis patients presenting an increased number of tumours, multiple tumour types and younger age at diagnosis. A comparison of the tumour microenvironment in sporadic and NF2-related schwannomatosis tumours is therefore required to underpin the development of immunotherapeutic targets, identify the possibility of extrapolating ex vivo data from sporadic vestibular schwannoma to NF2-related schwannomatosis and help inform clinical trial design with the feasibility of co-recruiting sporadic and NF2-related schwannomatosis patients. This study drew together bulk transcriptomic data from three published Affymetrix microarray datasets to compare the gene expression profiles of sporadic and NF2-related schwannomatosis vestibular schwannoma and subsequently deconvolved to predict the abundances of distinct tumour immune microenvironment populations. Data were validated using quantitative PCR and Hyperion imaging mass cytometry. Comparative bioinformatic analyses revealed close similarities in NF2-related schwannomatosis and sporadic vestibular schwannoma tumours across the three datasets. Significant inflammatory markers and signalling pathways were closely matched in NF2-related schwannomatosis and sporadic vestibular schwannoma, relating to the proliferation of macrophages, angiogenesis and inflammation. Bulk transcriptomic and imaging mass cytometry data identified macrophages as the most abundant immune population in vestibular schwannoma, comprising one-third of the cell mass in both NF2-related schwannomatosis and sporadic tumours. Importantly, there were no robust significant differences in signalling pathways, gene expression, cell type abundance or imaging mass cytometry staining between NF2-related schwannomatosis and sporadic vestibular schwannoma. These data indicate strong similarities in the tumour immune microenvironment of NF2-related schwannomatosis and sporadic vestibular schwannoma.

Słowa kluczowe:
tumour microenvironment, vestibular schwannoma, tumour-associated macrophages, NF2, NF2-related schwannomatosis

Afiliacje autorów:
Gregory Grace E. - inna afiliacja
Jones Adam P. - inna afiliacja
Haley Michael J. - inna afiliacja
Hoyle C. - inna afiliacja
Zeef Leo A. H. - inna afiliacja
Lin I. - inna afiliacja
Coope David J. - inna afiliacja
King Andrew T. - inna afiliacja
Evans D. G. - inna afiliacja
Paszek P. - IPPT PAN
Couper Kevin N. - inna afiliacja
Brough D. - inna afiliacja
Pathmanaban Omar N. - inna afiliacja
2.  Wang A., Cheng C., Lin I., Lu F., Tsai H., Lin C., Yang C., Pan P., Kuan C., Chen Y., Lin Y., Chang C., Wu Y., Kurniawan T., Lin C., Wo Andrew M., Chen L., A novel DNA selection and direct extraction process and its application in DNA recombination, Electrophoresis, ISSN: 0173-0835, DOI: 10.1002/elps.201000449, Vol.32, pp.423-430, 2011

In the conventional bench-top approach, the DNA recombination process is time- and effort-consuming due to laborious procedures lasting from several hours to a day. A novel DNA selection and direct extraction process has been proposed, integrated and tested on chip. The integrative microfluidic chip can perform the whole procedure of DNA recombination, including DNA digestion, gel electrophoresis, DNA extraction and insert-vector ligation within 1 h. In this high-throughput design, the manual gel cutting was replaced by an automatic processing system that performed high-quality and high-recovery efficiency in DNA extraction process. With no need of gel-dissolving reagents and manipulation, the application of selection and direct extraction process could significantly eliminate the risks from UV and EtBr and also facilitate DNA recombination. Reliable output with high success rate of cloning has been achieved with a significant reduction in operational hazards, required materials, efforts and time.

Słowa kluczowe:
Chip integration, DNA recombination, DNA selection and extraction , Microfluidics

Afiliacje autorów:
Wang A. - National Taiwan University (TW)
Cheng C. - inna afiliacja
Lin I. - inna afiliacja
Lu F. - inna afiliacja
Tsai H. - inna afiliacja
Lin C. - inna afiliacja
Yang C. - inna afiliacja
Pan P. - inna afiliacja
Kuan C. - inna afiliacja
Chen Y. - inna afiliacja
Lin Y. - inna afiliacja
Chang C. - inna afiliacja
Wu Y. - inna afiliacja
Kurniawan T. - inna afiliacja
Lin C. - inna afiliacja
Wo Andrew M. - inna afiliacja
Chen L. - inna afiliacja

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