Instytut Podstawowych Problemów Techniki
Polskiej Akademii Nauk


Zuzanna Wyrwa

Ostatnie publikacje
1.  Florek E., Witkowska M., Szukalska M., Richter M., Trzeciak T., Miechowicz I., Marszałek A., Piekoszewski W., Wyrwa Z., Giersig M., Oxidative Stress in Long-Term Exposure to Multi-Walled Carbon Nanotubes in Male Rats, Antioxidants, ISSN: 2076-3921, DOI: 10.3390/antiox12020464, Vol.12, No.464, pp.1-25, 2023


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Open AccessArticle
Oxidative Stress in Long-Term Exposure to Multi-Walled Carbon Nanotubes in Male Rats
by Ewa Florek
1,* [ORCID] , Marta Witkowska
2,3, Marta Szukalska
1 [ORCID] , Magdalena Richter
4 [ORCID] , Tomasz Trzeciak
4, Izabela Miechowicz
5 [ORCID] , Andrzej Marszałek
6 [ORCID] , Wojciech Piekoszewski
7 [ORCID] , Zuzanna Wyrwa
1 and Michael Giersig
Laboratory of Environmental Research, Department of Toxicology, Poznan University of Medical Sciences, 60-631 Poznan, Poland
Faculty of Chemistry, Adam Mickiewicz University, 61-614 Poznan, Poland
Centre for Advanced Technologies, Adam Mickiewicz University, 61-614 Poznan, Poland
Department of Orthopedics and Traumatology, Poznan University of Medical Sciences, 61-545 Poznan, Poland
Department of Computer Science and Statistics, Poznan University of Medical Sciences, 60-806 Poznan, Poland
Oncologic Pathology and Prophylaxis, Greater Poland Cancer Centre, Poznan University of Medical Sciences, 61-866 Poznan, Poland
Department of Analytical Chemistry, Faculty of Chemistry, Jagiellonian University, 30-387 Krakow, Poland
Department of Theory of Continuous Media and Nanostructures, Institute of Fundamental Technological Research, Polish Academy of Sciences, 02-106 Warsaw, Poland
Author to whom correspondence should be addressed.
Antioxidants 2023, 12(2), 464;
Received: 13 December 2022 / Revised: 7 February 2023 / Accepted: 10 February 2023 / Published: 12 February 2023
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Multi-walled carbon nanotubes (MWCNTs) serve as nanoparticles due to their size, and for that reason, when in contact with the biological system, they can have toxic effects. One of the main mechanisms responsible for nanotoxicity is oxidative stress resulting from the production of intracellular reactive oxygen species (ROS). Therefore, oxidative stress biomarkers are important tools for assessing MWCNTs toxicity. The aim of this study was to evaluate the oxidative stress of multi-walled carbon nanotubes in male rats. Our animal model studies of MWCNTs (diameter ~15–30 nm, length ~15–20 μm) include measurement of oxidative stress parameters in the body fluid and tissues of animals after long-term exposure. Rattus Norvegicus/Wistar male rats were administrated a single injection to the knee joint at three concentrations: 0.03 mg/mL, 0.25 mg/mL, and 0.5 mg/mL. The rats were euthanized 12 and 18 months post-exposure by drawing blood from the heart, and their liver and kidney tissues were removed. To evaluate toxicity, the enzymatic activity of total protein (TP), reduced glutathione (GSH), glutathione S–transferase (GST), thiobarbituric acid reactive substances (TBARS), Trolox equivalent antioxidant capacity (TEAC), nitric oxide (NO), and catalase (CAT) was measured and histopathological examination was conducted. Results in rat livers showed that TEAC level was decreased in rats receiving nanotubes at higher concentrations. Results in kidneys report that the level of NO showed higher concentration after long exposure, and results in animal serums showed lower levels of GSH in rats exposed to nanotubes at higher concentrations. The 18-month exposure also resulted in a statistically significant increase in GST activity in the group of rats exposed to nanotubes at higher concentrations compared to animals receiving MWCNTs at lower concentrations and compared to the control group. Therefore, an analysis of oxidative stress parameters can be a key indicator of the toxic potential of multi-walled carbon nanotubes.

Słowa kluczowe:
multi-walled carbon nanotubes,oxidative stress parameters,rats,long-term toxicity

Afiliacje autorów:
Florek E. - Poznan University of Medical Sciences (PL)
Witkowska M. - inna afiliacja
Szukalska M. - inna afiliacja
Richter M. - inna afiliacja
Trzeciak T. - Poznan University of Medical Sciences (PL)
Miechowicz I. - inna afiliacja
Marszałek A. - Nicolaus Copernicus University (PL)
Piekoszewski W. - inna afiliacja
Wyrwa Z. - inna afiliacja
Giersig M. - IPPT PAN

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